Emergent spotted fever group Rickettsiae infections among hard ticks in Islamic Republic of Iran
CONCLUSION: Considering the discovery of infected ticks in the Islamic Republic of Iran, there is a need to establish a tick control programme in the country, paying attention to populations at high-risk.PMID:38491900 | DOI:10.26719/emhj.24.030 (Source: Eastern Mediterranean Health Journal)
Source: Eastern Mediterranean Health Journal - March 16, 2024 Category: Middle East Health Authors: Mohammad Bagher Ghavami Zohreh Alibabaei Mohammad Reza Jamavar Behrooz Taghiloo Source Type: research

Viruses, Vol. 16, Pages 456: Direct Evidence of Powassan Virus Vertical Transmission in Ixodes scapularis in Nature
. Ciota Powassan virus (POWV) is a tick-borne flavivirus endemic in North America and Russia. Experimental infections with POWV have confirmed horizontal, transstadial, vertical, and cofeeding transmission routes for potential virus maintenance. In the field, vertical transmission has never been observed. During New York State tick-borne pathogen surveillance, POWV RNA and/or infectious POWV was detected in five pools of questing Ixodes scapularis larvae. Additionally, engorged female I. scapularis adults were collected from hunter-harvested white-tailed deer (Odocoileus virginianus) in a region with relatively high ti...
Source: Viruses - March 16, 2024 Category: Virology Authors: Rachel E. Lange Melissa A. Prusinski Alan P. Dupuis Alexander T. Ciota Tags: Brief Report Source Type: research

VitroJet: new features and case studies
Single-particle cryo-electron microscopy has become a widely adopted method in structural biology due to many recent technological advances in microscopes, detectors and image processing. Before being able to inspect a biological sample in an electron microscope, it needs to be deposited in a thin layer on a grid and rapidly frozen. The VitroJet was designed with this aim, as well as avoiding the delicate manual handling and transfer steps that occur during the conventional grid-preparation process. Since its creation, numerous technical developments have resulted in a device that is now widely utilized in multiple laborat...
Source: Acta Crystallographica Section D - March 15, 2024 Category: Biochemistry Authors: Henderikx, R.J.M. Mann, D. Domanska, A. Dong, J. Shahzad, S. Lak, B. Filopoulou, A. Ludig, D. Grininger, M. Momoh, J. Laanto, E. Oksanen, H.M. Bisikalo, K. Williams, P.A. Butcher, S.J. Peters, P.J. Beulen, B.W.A.M.M. Tags: cryo-EM VitroJet ice thickness pin printing jet vitrification research papers Source Type: research

Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: a study in swine
J Virol Methods. 2024 Mar 11:114915. doi: 10.1016/j.jviromet.2024.114915. Online ahead of print.ABSTRACTCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species reported to be exposed to CCHFV in the field, including wild boar (Sus scrofa). However, development and validation of...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Caroline Bost Sabrina Castro-Scholten Balal Sadeghi David Cano-Terriza Mario Fr ías Sa úl Jiménez-Ruiz Martin H Groschup Ignacio Garc ía-Bocanegra Kerstin Fischer Source Type: research

Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: a study in swine
J Virol Methods. 2024 Mar 11:114915. doi: 10.1016/j.jviromet.2024.114915. Online ahead of print.ABSTRACTCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species reported to be exposed to CCHFV in the field, including wild boar (Sus scrofa). However, development and validation of...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Caroline Bost Sabrina Castro-Scholten Balal Sadeghi David Cano-Terriza Mario Fr ías Sa úl Jiménez-Ruiz Martin H Groschup Ignacio Garc ía-Bocanegra Kerstin Fischer Source Type: research