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Long non-coding RNA Gm11974 aggravates oxygen –glucose deprivation-induced injury via miR-122-5p/SEMA3A axis in ischaemic stroke
This study aimed to investigate the role and potential mechanism of lncRNA Gm11974 in ischaemic stroke. Mouse  neuroblastoma N2a cells were treated with oxygen–glucose deprivation (OGD). The levels of Gm11974, microRNA-122-5p (miR-122-5p) and semaphorin 3A (SEMA3A) were detected by quantitative real-time PCR (qRT-PCR) or western blot. Cell viability and apoptosis were determined by 3-(4,5-dimethyl-2-thia zolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, Caspase-3 Assay Kit and flow Cytometry. The levels of oxidative stress indicators were measured by using commercial kits. The relationship between miR-122-5p and ...
Source: Metabolic Brain Disease - August 2, 2021 Category: Neurology Source Type: research

SIRT1/PGC-1 α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells
This study is designed to investigate the protective effect of MGF treatment in the setting of cerebral IRI and to elucidate the potential mechanisms. We first evaluated the toxicity of MGF and chose the safe concentrations for the following experiments. MGF exerted obvious neuroprotection against hypoxia/reoxygenation (HR)-induced injury, indicated by restored cell viability and cell morphology, decreased lactate dehydrogenase (LDH) release and reactive oxygen species generation. MGF also restored the protein expressions of SIRT1, PGC-1α, Nrf2, NQO1, HO-1, NRF1, UCP2, and Bcl2 down-regulated by HR treatment. However, SIR...
Source: European Journal of Pharmacology - June 11, 2021 Category: Drugs & Pharmacology Authors: Mengfan Chen Zheng Wang Wenying Zhou Chenxi Lu Ting Ji Wenwen Yang Zhenxiao Jin Ye Tian Wangrui Lei Songdi Wu Qi Fu Zhen Wu Xue Wu Mengzhen Han Minfeng Fang Yang Yang Source Type: research

Neuroprotective effects of Senkyunolide I against glutamate-induced cells death by attenuating JNK/caspase-3 activation and apoptosis
Biomed Pharmacother. 2021 May 24;140:111696. doi: 10.1016/j.biopha.2021.111696. Online ahead of print.ABSTRACTGlutamate-induced neurotoxicity is one of the most important pathogenic mechanisms in neurological diseases and is widely used as an in vitro model for ischemic stroke. Senkyunolide I (SEI), an active constituent derived from traditional Chinese medicine Ligusticum chuanxiong Hort. and Angelica sinensis (Oliv.) Diels, has been shown to have beneficial effects against focal cerebral ischemia-reperfusion in rats. However, the mechanisms underlying SEI-mediated neuroprotection remain not well understood. Thus, we expl...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - May 27, 2021 Category: Drugs & Pharmacology Authors: Min Wang Hideki Hayashi Ichiro Horinokita Mayumi Asada Yui Iwatani Jian-Xun Liu Norio Takagi Source Type: research

Increase of Cry 1 expression is a common phenomenon of the disturbed circadian clock in ischemic stroke and opioid addiction
Biochem Biophys Res Commun. 2021 Apr 21;558:8-13. doi: 10.1016/j.bbrc.2021.04.053. Online ahead of print.ABSTRACTIncreasing evidences suggest the involvement of disrupted circadian clock in various pathologies including stroke and substance abuse. Here we took an attempt to do a comparative study on the regulation of circadian clock gene expression under two pathological circumstances - Opioid addiction and Ischemic stroke in the same cell line model (human neuroblastoma SH-SY5Y cells). To mimic in vivo ischemic stroke condition cells were placed in a hypoxia chamber and incubated for 10 h in balanced salt solution lacking...
Source: Biochemical and Biophysical Research communications - April 24, 2021 Category: Biochemistry Authors: Kaninika Roy Daytee Maji Ishani Deb Source Type: research

Discovery of neuroprotective agents that inhibit human prolyl hydroxylase PHD2
Bioorg Med Chem. 2021 Mar 24;38:116115. doi: 10.1016/j.bmc.2021.116115. Online ahead of print.ABSTRACTProlyl hydroxylase (PHD) enzymes play a critical role in the cellular responses to hypoxia through their regulation of the hypoxia inducible factor α (HIF-α) transcription factors. PHD inhibitors show promise for the treatment of diseases including anaemia, cardiovascular disease and stroke. In this work, a pharmacophore-based virtual high throughput screen was used to identify novel potential inhibitors of human PHD2. Two moderately potent new inhibitors were discovered, with IC50 values of 4 μM and 23 μM respectively...
Source: Bioorganic and Medicinal Chemistry - April 16, 2021 Category: Chemistry Authors: Nicole L Richardson Laura J O'Malley Daniel Weissberger Anthony Tumber Christopher J Schofield Renate Griffith Nicole M Jones Luke Hunter Source Type: research

Effects of uric acid on oxidative and nitrosative stress and other related parameters in SH-SY5Y human neuroblastoma cells.
Uric acid (UA) is the end -product of purine catabolism in humans and higher primates and comprises about 65% of the total antioxidant capacity of plasma [1]. Urate circulates in humans at concentrations that are near its limit of solubility and many times higher than those in most other mammals. In patients with ischemic stroke, the most common type of stroke which is a leading cause of disability and the second most common cause of dementia, UA reduces the incidence of early clinical deterioration and improves outcomes compared to placebo [2].
Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - December 30, 2020 Category: Biomedical Science Authors: Frederic M ármol, Juan Sanchez, Albert Martínez-Pinteño Source Type: research

LL-00066471, a novel positive allosteric modulator of α7 nicotinic acetylcholine receptor ameliorates cognitive and sensorimotor gating deficits in animal models: Discovery and preclinical characterization.
Abstract α7 nicotinic acetylcholine receptor (α7 nAChR) is an extensively validated target for several neurological and psychiatric conditions namely, dementia and schizophrenia, owing to its vital roles in cognition and sensorimotor gating. Positive allosteric modulation (PAM) of α7 nAChR represents an innovative approach to amplify endogenous cholinergic signaling in a temporally restricted manner in learning and memory centers of brain. α7 nAChR PAMs are anticipated to side-step burgeoning issues observed with several clinical-stage orthosteric α7 nAChR agonists, related to selectivity, tolerance/tachyphyl...
Source: European Journal of Pharmacology - October 27, 2020 Category: Drugs & Pharmacology Authors: Verma MK, Goel RN, Bokare AM, Dandekar MP, Koul S, Desai S, Tota S, Singh N, Nigade PB, Patil VB, Modi D, Mehta M, Gundu J, Walunj SS, Karche NP, Sinha N, Kamboj RK, Palle VP Tags: Eur J Pharmacol Source Type: research

LncRNA FOXD3-AS1 knockdown protects against cerebral ischemia/reperfusion injury via miR-765/BCL2L13 axis.
CONCLUSIONS: We verified a critical signaling pathway of FOXD3-AS1/miR-765/BCL2L13 in regulating cerebral ischemia/reperfusion injury. PMID: 33068927 [PubMed - as supplied by publisher]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - October 14, 2020 Category: Drugs & Pharmacology Authors: Lu Y, Han Y, He J, Zhou B, Fang P, Li X Tags: Biomed Pharmacother Source Type: research

miR ‑183‑5p attenuates cerebral ischemia injury by negatively regulating PTEN.
miR‑183‑5p attenuates cerebral ischemia injury by negatively regulating PTEN. Mol Med Rep. 2020 Sep 07;: Authors: Zhu L, Zhou X, Li S, Liu J, Yang J, Fan X, Zhou S Abstract Cerebral ischemia is a common cerebrovascular disease caused by the occlusion of a cerebral blood vessel. MicroRNAs (miRNAs/miRs) are emerging regulators of various human diseases, including cerebral ischemia. Upregulation of miR‑183‑5p has been reported to alleviate liver injury induced by ischemia‑reperfusion (I/R). However, the effect of miR‑183‑5p on cerebral ischemia injury remains unknown. The present study evalu...
Source: Molecular Medicine Reports - September 10, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

N-hydroxy-N'-(4-butyl-2-methylphenyl)-formamidine attenuates oxygen-glucose deprivation and reoxygenation-induced cerebral ischemia-reperfusion injury via regulation of microRNAs.
Abstract Cerebral ischemia-reperfusion injury is a common complication that occurs during stroke treatment. Increasingly, microRNAs have been found to participate in the modulation of neuron function; however, the role of microRNAs in cerebral ischemia-reperfusion injury remains unclear. We developed a mechanism of cerebral ischemia-reperfusion injury using a cellular model of oxygen-glucose deprivation and reoxygenation-induced injury in human neuroblastoma SH-SY5Y cells. We found that treatment of oxygen-glucose deprivation and reoxygenation promoted the apoptosis of SH-SY5Y cells. Analysis of microRNAs sequenci...
Source: Journal of Integrative Neuroscience - June 29, 2020 Category: Neuroscience Authors: Yang T, Wang D, Qu Y, Wang Y, Feng Y, Yang Y, Luo Q, Sun X, Yu G, He J, Sun Z, Zhu Y Tags: J Integr Neurosci Source Type: research

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