Filtered By:
Therapy: Dialysis

This page shows you your search results in order of relevance.

Order by Relevance | Date

Total 46 results found since Jan 2013.

Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs
In this study, As1411 aptamer-conjugated liposomes were used for targeted siRNA delivery against the COL1A1 gene in colorectal cancer (CRC) cells. Cationic liposomes were synthesized and siRNA loading and conjugation of aptamer were confirmed by gel shift assay and spectrophotometry method. Release of siRNA from liposomes was assessed using dialysis method. Binding and uptake of aptamer-conjugated liposomes to and into cancer cells was assessed by fluorescence microscopy and flowcytometry. Gene expression was evaluated using qRT-PCR. Cell viability, chemosensitivity and apoptosis were determined by MTT assay and Annexin/PI...
Source: Colloids and Surfaces - July 29, 2022 Category: Biotechnology Authors: Hamed Manoochehri Akram Jalali Hamid Tanzadehpanah Amir Taherkhani Rezvan Najafi Source Type: research

Evaluation of liver specific ionizable lipid nanocarrierin the delivery of siRNA
Chem Phys Lipids. 2022 May 24:105207. doi: 10.1016/j.chemphyslip.2022.105207. Online ahead of print.ABSTRACTHepcidin, a key regulator of iron homeostasis, has been implicated in the pathogenesis of various iron-related diseases. Although small interfering RNA (siRNA) are potent to modulate the expression of hepcidin, their bioavailability remains a major issue. The β-galactopyranoside-conjugated liposomes (GAL-liposome) targeting liver synthesized hepcidin were prepared by thin lipid film hydration method to encapsulate siRNA and the conjugation of β-galactopyranoside to the lipid nanocarrier was achieved by covalent che...
Source: Chemistry and Physics of Lipids - May 27, 2022 Category: Lipidology Authors: Shilpa Rana Archana Bhatnagar Suman Singh Nirmal Prabhakar Source Type: research

Role of Small Interfering RNA Silencing Protein Kinase C ‐α Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats
This study aims to explore the effect of PKC‐α gene silencing on the occurrence of ultrafiltration failure (UFF) in peritoneal dialysis (PD) rats. Forty‐eight male SD rats were collected to establish 5/6 renal resection uremic and uremic PD rats models. Rats were assigned into control, sham operation, uremia, PD‐2 W (peritoneal dialysis for 2 weeks), PD‐4 W (peritoneal dialysis for 4 weeks), negative control (NC) (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg blank plasmid into abdominal cavity) and PKC‐α siRNA (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg PKC‐α siRNA into abdomi...
Source: Journal of Cellular Biochemistry - May 9, 2017 Category: Biochemistry Authors: Zhi ‐Wei Sun, Jian Wang, Min Weng, Jian‐Zhong Tang, Jun‐Feng Wang, Jian Xu, Ling Lin, Hong‐Ling Yuan Tags: Article Source Type: research

Lipopolysaccharide (LPS)-induced autophagy is involved in the restriction of Escherichia coli in peritoneal mesothelial cells
Conclusions: Our findings demonstrated for the first time that LPS-induced autophagy in peritoneal mesothelial cells could enhance the intracellular bactericidal activity and the co-localization of E.coli with autophagosomes. The activation of TLR4 signaling was involved in this process. These results indicate that LPS-induced autophagy may be a cell-autonomous defense mechanism triggered in peritoneal mesothelial cells in response to E.coli infection.
Source: BMC Microbiology - Latest articles - November 13, 2013 Category: Microbiology Authors: Juan WangXiaoran FengYoujia ZengJinjin FanJuan WuZhijian LiXinhui LiuRong HuangFengxian HuangXueqing YuXiao Yang Source Type: research

The effect of FoxO1 on the proliferation of rat mesangial cells under high glucose conditions
Conclusions Overexpression of FoxO1 caused upregulation of p27, which promoted cell cycle arrest and inhibited hyperproliferation of MCs induced by HG. Degradation of FoxO1 caused an increase in p27 and stimulated MC proliferation. These findings unveil part of the molecular mechanism of FoxO1 regulation of MC hyperproliferation induced by HG.
Source: Nephrology Dialysis Transplantation - September 24, 2014 Category: Urology & Nephrology Authors: Liu, F., Ma, X.-J., Wang, Q.-Z., Zhao, Y.-Y., Wu, L.-N., Qin, G.-J. Tags: BASIC SCIENCE Source Type: research

Autophagy promotes fibrosis and apoptosis in the peritoneum during long ‐term peritoneal dialysis
In this study, we found that the coincidence of elevated TGF‐β1 expression, autophagy, apoptosis and fibrosis in peritoneal membrane from patients with peritoneal dialysis. The peritoneal membranes from patients were performed with immunocytochemistry and transmission electron microscopy. Human peritoneal mesothelial cells were treated with 1.5%, 2.5% and 4.25% HGPDS for 24 hrs; Human peritoneal mesothelial cells pre‐treated with TGF‐β1 (10 ng/ml) or transfected with siRNA Beclin1 were treated with 4.25% HGPDS or vehicle for 24 hrs. We further detected the production of TGF‐β1, activation of TGF‐β1/Smad2/3 si...
Source: Journal of Cellular and Molecular Medicine - October 27, 2017 Category: Molecular Biology Authors: Jingjing Wu, Changying Xing, Li Zhang, Huijuan Mao, Xuguan Chen, Mingxing Liang, Fang Wang, Haibin Ren, Hongqing Cui, Aiqin Jiang, Zibin Wang, Meijuan Zou, Yong Ji Tags: Original Article Source Type: research

Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis.
In this study, we found that the coincidence of elevated TGF-β1 expression, autophagy, apoptosis and fibrosis in peritoneal membrane from patients with peritoneal dialysis. The peritoneal membranes from patients were performed with immunocytochemistry and transmission electron microscopy. Human peritoneal mesothelial cells were treated with 1.5%, 2.5% and 4.25% HGPDS for 24 hrs; Human peritoneal mesothelial cells pre-treated with TGF-β1 (10 ng/ml) or transfected with siRNA Beclin1 were treated with 4.25% HGPDS or vehicle for 24 hrs. We further detected the production of TGF-β1, activation of TGF-β1/Smad2/3 signalling, ...
Source: J Cell Mol Med - October 27, 2017 Category: Molecular Biology Authors: Wu J, Xing C, Zhang L, Mao H, Chen X, Liang M, Wang F, Ren H, Cui H, Jiang A, Wang Z, Zou M, Ji Y Tags: J Cell Mol Med Source Type: research

Modulation of A-type K(+) channels by the short-chain cobrotoxin through the protein kinase C-delta isoform decreases membrane excitability in dorsal root ganglion neurons.
In this study, we identified the functional role of cobrotoxin, the short-chain α-neurotoxin isolated from Naja atra venom, which acts in the regulation of the transient A-type K(+) currents (I(A)) and membrane excitability in dorsal root ganglion (DRG) neurons via the activation of the muscarinic M3 receptor (M3R). Our results showed that cobrotoxin increased I(A) in a concentration-dependent manner, whereas the sustained delayed rectifier K(+) currents (I(DR)) were not affected. Cobrotoxin did not affect the activation of I(A) markedly, however, it shifted the inactivation curve significantly in the depolarizing directi...
Source: Biochemical Pharmacology - February 19, 2013 Category: Drugs & Pharmacology Authors: Guo Q, Jiang YJ, Jin H, Jiang XH, Gu B, Zhang YM, Wang JG, Qin ZH, Tao J Tags: Biochem Pharmacol Source Type: research

Cell signalling
Conclusions: Taken together, it is likely that klotho protects against renal fibrotic process accompanied by down-regulation of fibrotic markers, counteracting to TGF-β, and one of possible mechanism mediating translocation of Na/K ATPase, resulting in Ca+ channel stabilization/alternation of Ca+ ion concentration. Klotho should be involved in the accentuation of the progression of renal fibrosis in CKD.
Source: Nephrology Dialysis Transplantation - May 10, 2013 Category: Urology & Nephrology Authors: Tsuchiya, K., Shiohira, S., Sugiura, H., Suzuki, M., Okano, K., Nitta, K., Kaesler, N., Immendorf, S., Ouyang, C., Carmeliet, P., Floege, J., Kruger, T., Schlieper, G., Georgescu, A., Kalucka, J., Olbrich, S., Baumgartl, J., Hackenbeck, T., Eckardt, K.-U. Tags: Abstracts Source Type: research

Cell signalling and apoptosis
Conclusions: These results suggest that activation of S1P signaling mediated by S1PR-3 results in chronic pathological fibrosis, such as in chronic kidney disease (CKD).
Source: Nephrology Dialysis Transplantation - May 21, 2014 Category: Urology & Nephrology Authors: Shiohira, S., Yoshida, T., Sugiura, H., Nishida, M., Nitta, K., Tsuchiya, K., Grampp, S., Goppelt-Strube, M., Eckardt, K. U., Schodel, J., Kang, S. W., Kim, Y., Seo, S.-K., Kim, T., Ong, S., Yang, W. S., Han, N. J., Lee, J. M., Baek, C. H., Park, S.-K., K Tags: Monday, June 2nd, 2014: Posters Source Type: research

BMP‐9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway
Abstract The process of vascular calcification shares many similarities with that of physiological skeletal mineralization, and involves the deposition of hydroxyapatite crystals in arteries. However, the cellular mechanisms responsible have yet to be fully explained. Bone morphogenetic protein (BMP‐9) has been shown to exert direct effects on both bone development and vascular function. In the present study, we have investigated the role of BMP‐9 in vascular smooth muscle cell (VSMC) calcification. Vessel calcification in chronic kidney disease (CKD) begins pre‐dialysis, with factors specific to the dialysis milieu ...
Source: Journal of Cellular and Molecular Medicine - October 9, 2014 Category: Molecular Biology Authors: Dongxing Zhu, Neil Charles Wallace Mackenzie, Catherine M. Shanahan, Rukshana C. Shroff, Colin Farquharson, Vicky Elisabeth MacRae Tags: Original Article Source Type: research

BMP-9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway.
Abstract The process of vascular calcification shares many similarities with that of physiological skeletal mineralization, and involves the deposition of hydroxyapatite crystals in arteries. However, the cellular mechanisms responsible have yet to be fully explained. Bone morphogenetic protein (BMP-9) has been shown to exert direct effects on both bone development and vascular function. In the present study, we have investigated the role of BMP-9 in vascular smooth muscle cell (VSMC) calcification. Vessel calcification in chronic kidney disease (CKD) begins pre-dialysis, with factors specific to the dialysis mili...
Source: J Cell Mol Med - October 9, 2014 Category: Molecular Biology Authors: Zhu D, Mackenzie NC, Shanahan CM, Shroff RC, Farquharson C, MacRae VE Tags: J Cell Mol Med Source Type: research