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Acetyl-11-keto-β-boswellic acid regulates the repair of rat sciatic nerve injury by promoting the proliferation of Schwann cells
This study aimed to study the effects of acetyl-11-keto-β-boswellic acid (AKBA) on the regeneration of injured peripheral nerves and the ability of the extracellular signal-regulated kinase（ERK） signaling pathway to regulate the proliferation of Schwann cells and the formation of myelin.Main methodsA sciatic nerve crush injury model rats were randomly divided into the model control, low-, medium-, and high-dose AKBA groups. The repair of myelin damage was observed through Luxol Fast Blue staining and the expression of neurofilament-200 (NF200) protein was detected through immunohistochemical tests. The relative expres...
Source: Life Sciences - October 11, 2019 Category: Biology Source Type: research

Oxidized base 8-oxoguanine, a product of DNA repair processes, contributes to dendritic cell activation.
Abstract A growing body of evidence suggests that elevated levels of reactive oxygen species (ROS) in the airways caused by exposure to gas phase pollutants or particulate matter are able to activate dendritic cells (DCs); however, the exact mechanisms are still unclear. When present in excess, ROS can modify macromolecules including DNA. One of the most abundant DNA base lesions is 7,8-dihydro-8-oxoguanine (8-oxoG), which is repaired by the 8-oxoguanine DNA glycosylase 1 (OGG1)-initiated base excision repair (BER) pathway. Studies have also demonstrated that in addition to its role in repairing oxidized purines, ...
Source: Free Radical Biology and Medicine - August 9, 2019 Category: Biology Authors: Pázmándi K, Sütő M, Fekete T, Varga A, Boldizsár E, Boldogh I, Bácsi A Tags: Free Radic Biol Med Source Type: research

CHK1-CENP B/MAD2 is associated with mild oxidative damage-induced sex chromosome aneuploidy of male mouse embryos during in vitro fertilization.
Abstract A high incidence of aneuploidy is observed in in vitro fertilization (IVF)-derived embryos, but the formation and repair mechanisms are unknown. Here, we investigated the effects of slightly increased reactive oxygen species (ROS) produced by in vitro culture conditions on embryo aneuploidy and the roles of the spindle assembly checkpoint (SAC) protein, mitotic arrest-deficient 2 (MAD2), and the DNA damage response (DDR) protein, checkpoint kinase 1 (CHK1), in aneuploidy repair. By assessing chromosome abnormalities via DAPI staining, karyotype analysis and next-generation sequencing technology, we demons...
Source: Free Radical Biology and Medicine - April 27, 2019 Category: Biology Authors: Huang Y, Ha S, Li Z, Li J, Xiao W Tags: Free Radic Biol Med Source Type: research

Growth arrest and DNA-damage-inducible 45 beta (GADD45β) deletion suppresses testosterone-induced prostate hyperplasia in mice
Publication date: Available online 6 September 2018Source: Life SciencesAuthor(s): Se-Ra Park, Da-Young Jung, Tae-Won Kim, Chul-Ho Lee, Ju-Young JungAbstractAimGrowth arrest and DNA-damage-inducible 45 beta (GADD45β) is a member of the gene family associated with cell growth control, apoptosis, and DNA damage repair. The aim of present study was to determine the potential effects of GADD45β deletion on prostate hyperplasia progression.Main methodsLNCaP cells were incubated with testosterone propionate (1 μM) for 48 h and specific siRNA used to suppress GADD45β expression in vitro. For in vivo experiments, testoster...
Source: Life Sciences - September 6, 2018 Category: Biology Source Type: research

Axis of serotonin -pERK-YAP in liver regeneration
ConclusionA role of the 5-HT-pERK-YAP axis in liver regeneration emerged from our study and might be a potential target to promote regeneration and injury repair.
Source: Life Sciences - August 21, 2018 Category: Biology Source Type: research

Effects of the stimuli-dependent enrichment of 8-oxoguanine DNA glycosylase1 on chromatinized DNA
Publication date: September 2018Source: Redox Biology, Volume 18Author(s): Wenjing Hao, Tianyang Qi, Lang Pan, Ruoxi Wang, Bing Zhu, Leopoldo Aguilera-Aguirre, Zsolt Radak, Tapas K. Hazra, Spiros A. Vlahopoulos, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan BoldoghAbstract8-Oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an epigenetic mark and that consequently OGG1 acquire distinct roles in modulation o...
Source: Redox Biology - July 11, 2018 Category: Biology Source Type: research

PP2A regulates SCF-induced cardiac stem cell migration through interaction with p38 MAPK
Publication date: 15 December 2017 Source:Life Sciences, Volume 191 Author(s): Ying Wang, Yanli Xia, Dong Kuang, Yaqi Duan, Guoping Wang Aims Previous studies have shown that stem cell factor (SCF) induces the migration of cardiac stem cells (CSCs) and helps to repair myocardial infarctions. Earlier studies on the migration mechanism only focused on the activation of kinases; here, we aimed to explore the functional role of protein phosphatase 2A (PP2A) in SCF-induced CSC migration. Main methods CSCs were treated with SCF, PP2A enzymatic activity was measured, the phosphorylation levels of PP2A, p38 MAPK and cofilin were ...
Source: Life Sciences - October 14, 2017 Category: Biology Source Type: research

PARP-1 inhibition influences the oxidative stress response of the human lens
Publication date: Available online 7 March 2016 Source:Redox Biology Author(s): Andrew J.O. Smith, Simon S.R. Ball, Richard P. Bowater, I. Michael Wormstone Poly(ADP-ribose) polymerase-1 (PARP-1) is best characterised for its involvement in DNA repair. PARP-1 activity is also linked to cell fate, confounding its roles in maintaining genome integrity. The current study assessed the functional roles of PARP-1 within human lens cells in response to oxidative stress. The human lens epithelial cell line FHL124 and whole human lens cultures were used as experimental systems. Hydrogen peroxide (H2O2) was employed to induce ...
Source: Redox Biology - March 8, 2016 Category: Biology Source Type: research

Regulation of homologous recombinational repair by lamin B1 in radiation-induced DNA damage Research Communication
DNA double-strand breaks (DSBs) are the major lethal lesion induced by ionizing radiation (IR). RAD51-dependent homologous recombination (HR) is one of the most important pathways in DSB repair and genome integrity maintenance. However, the mechanism of HR regulation by RAD51 remains unclear. To understand the mechanism of RAD51-dependent HR, we searched for interacting partners of RAD51 by a proteomics analysis and identified lamin B1 in human cells. Lamins are nuclear lamina proteins that play important roles in the structural organization of the nucleus and the regulation of chromosome functions. Immunoblotting analyses...
Source: FASEB Journal - June 1, 2015 Category: Biology Authors: Liu, N.-A., Sun, J., Kono, K., Horikoshi, Y., Ikura, T., Tong, X., Haraguchi, T., Tashiro, S. Tags: Research Communication Source Type: research

Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia Research Communication
We studied the role of matrix metalloproteinase-10 (MMP-10) during skeletal muscle repair after ischemia using a model of femoral artery excision in wild-type (WT) and MMP-10 deficient (Mmp10–/–) mice. Functional changes were analyzed by small animal positron emission tomography and tissue morphology by immunohistochemistry. Gene expression and protein analysis were used to study the molecular mechanisms governed by MMP-10 in hypoxia. Early after ischemia, MMP-10 deficiency resulted in delayed tissue reperfusion (10%, P < 0.01) and in increased necrosis (2-fold, P < 0.01), neutrophil (4-fold, P < 0.01)...
Source: FASEB Journal - March 2, 2015 Category: Biology Authors: Gomez-Rodriguez, V., Orbe, J., Martinez-Aguilar, E., Rodriguez, J. A., Fernandez-Alonso, L., Serneels, J., Bobadilla, M., Perez-Ruiz, A., Collantes, M., Mazzone, M., Paramo, J. A., Roncal, C. Tags: Research Communication Source Type: research

8-Oxoguanine DNA glycosylase-1-mediated DNA repair is associated with Rho-GTPase activation and smooth muscle α-actin polymerization.
Abstract Reactive oxygen species (ROS) are activators of cell signaling and modify cellular molecules, including DNA. 8-oxo-7,8-dihydro-guanine (8-oxoG) is one of the prominent lesions in oxidatively damaged DNA, whose accumulation is causally linked to various diseases and aging processes, while its etiological relevance is unclear. 8-oxoG is repaired by the 8-oxoguanine DNA glycosylase-1 (OGG1)- initiated DNA base excision repair pathway (BER). OGG1 binds free 8-oxoG and this complex functions as an activator of Ras family GTPases. Here we examined whether OGG1-initiated BER is associated with the activation of ...
Source: Free Radical Biology and Medicine - March 26, 2014 Category: Biology Authors: Luo J, Hosoki K, Bacsi A, Radak Z, Hegde ML, Sur S, Hazra TK, Brasier AR, Ba X, Boldogh I Tags: Free Radic Biol Med Source Type: research

ErbB3 binding protein-1 (Ebp1) controls proliferation and myogenic differentiation of muscle stem cells.
Abstract Satellite cells are resident stem cells of skeletal muscle, supplying myoblasts for postnatal muscle growth, hypertrophy and repair. Of the complex regulatory networks that control satellite cells, the EGF-family of ligands contributes. Here we investigated the role of ErbB3 binding protein-1 (Ebp1) in regulation of myogenic stem cell proliferation and differentiation. Ebp1 is a well-conserved DNA/RNA binding protein that is implicated in cell growth, apoptosis and differentiation in many cell types. Of the two main Ebp1 isoforms, only p48 was expressed in satellite cells and C2C12 myoblasts. While not pr...
Source: Developmental Biology - November 22, 2013 Category: Biology Authors: Figeac N, Serralbo O, Marcelle C, Zammit PS Tags: Dev Biol Source Type: research

Pitx genes are redeployed in adult myogenesis where they can act to promote myogenic differentiation in muscle satellite cells.
Abstract Skeletal muscle retains a resident stem cell population called satellite cells. Although mitotically quiescent in mature muscle, satellite cells can be activated to produce myoblast progeny to generate myonuclei for skeletal muscle homoeostasis, hypertrophy and repair. Regulation of satellite cell function in adult requires redeployment of many of the regulatory networks fundamental to developmental myogenesis. Involved in such control of muscle stem cell fate in embryos are members of the Pitx gene family of bicoid-class homeodomain proteins. Here, we investigated the expression and function of all three...
Source: Developmental Biology - May 1, 2013 Category: Biology Authors: Knopp P, Figeac N, Fortier M, Moyle L, Zammit PS Tags: Dev Biol Source Type: research