Effects of the stimuli-dependent enrichment of 8-oxoguanine DNA glycosylase1 on chromatinized DNA

Publication date: September 2018Source: Redox Biology, Volume 18Author(s): Wenjing Hao, Tianyang Qi, Lang Pan, Ruoxi Wang, Bing Zhu, Leopoldo Aguilera-Aguirre, Zsolt Radak, Tapas K. Hazra, Spiros A. Vlahopoulos, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan BoldoghAbstract8-Oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an epigenetic mark and that consequently OGG1 acquire distinct roles in modulation of gene expression. In support, lack of functional OGG1 in Ogg1-/- mice led to an altered expression of genes including those responsible for the aberrant innate and adaptive immune responses and susceptibility to metabolic disorders. Therefore, the present study examined stimulus-driven OGG1-DNA interactions at whole genome level using chromatin immunoprecipitation (ChIP)-coupled sequencing, and the roles of OGG1 enriched on the genome were validated by molecular and system-level approaches. Results showed that signaling levels of cellular ROS generated by TNFα, induced enrichment of OGG1 at specific sites of chromatinized DNA, primarily in the regulatory regions of genes. OGG1-ChIP-ed genes are associated with important cellular and biological processes and OGG1 enrichment was limited to a time scale required for immediate cellular responses. Preventi...
Source: Redox Biology - Category: Biology Source Type: research

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Publication date: 15 April 2019Source: Chemical Engineering Journal, Volume 362Author(s): Jin Qu, Xin Zhao, Yongping Liang, Yameng Xu, Peter X. Ma, Baolin GuoAbstractBesides preventing wound infection, novel wound dressing materials are highly expected to exhibit the extraordinary wound repair and skin regeneration advantage. Herein, we designed a kind of multi-functional injectable hydrogel dressing, integrating the conductivity, desirable antioxidant ability and antibacterial property to meet increasing requirements of skin damage. By mixing the biocompatible polymer N-carboxyethyl chitosan (CEC) and oxidized hyaluronic ...
Source: Chemical Engineering Journal - Category: Chemistry Source Type: research
CONCLUSIONS: Zyxin is a crucial ingredient of the cellular mechanotransducing system and can modulate the gene expression. Given its clinical relevance, zyxin is also a promising target for the diagnosis and treatment of certain diseases. Understanding the role of zyxin in force sensing and gene expression regulating provides a compelling challenge for future biomechanics studies, and offers attractive evidence for zyxin as a potential diagnostic marker and therapeutic target for clinical diseases. PMID: 30657586 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
ConclusionFor patients who had undergone EVAR, type III endoleaks can present only decades later and pose a significant risk of aneurysmal rupture.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Publication date: March 2019Source: Molecular Immunology, Volume 107Author(s): Mónica Daniela Mora-Ruíz, Francisco Blanco-Favela, Adriana Karina Chávez Rueda, María Victoria Legorreta-Haquet, Luis Chávez-SánchezAbstractAcute myocardial infarction (AMI) is a leading cause of death worldwide. Myocardial necrosis generates damage signals and triggers an intense inflammatory response. Many cytokines that contribute to repair tissue can also cause adverse left ventricular remodeling and heart failure. Several studies have revealed that interleukin-17 (IL-17) is a cytokine with a potenti...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 18 January 2019Source: Seminars in Cell &Developmental BiologyAuthor(s): Bruno L. Marques, Gustavo A. Carvalho, Elis M.M. Freitas, Raphaela A. Chiareli, Thiago G. Barbosa, Armani G.P. Di Araújo, Yanley L. Nogueira, Raul I. Ribeiro, Ricardo C. Parreira, Mariana S. Vieira, Rodrigo R. Resende, Renato S. Gomez, Onésia C. Oliveira-Lima, Mauro C.X. PintoAbstractStroke consists of an abrupt reduction of cerebral blood flow resulting in hypoxia that triggers an excitotoxicity, oxidative stress, and neuroinflammation. After the ischemic process, neural precursor cells present in ...
Source: Seminars in Cell and Developmental Biology - Category: Cytology Source Type: research
In this study, scientists screened cells from old animals to identify any RBPs that change upon aging. The screening showed that one particular protein, Pumilio2 (PUM2), was highly induced in old animals. PUM2 binds mRNA molecules containing specific recognition sites. Upon its binding, PUM2 represses the translation of the target mRNAs into proteins. Using a systems genetics approach, the researchers then identified a new mRNA target that PUM2 binds. The mRNA encodes for a protein called Mitochondrial Fission Factor (MFF), and is a pivotal regulator of mitochondrial fission - a process by which mitochondria break u...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Authors: Ercin BS, Bicer A, Yigitturk G, Cinar C, Cavusoglu T, Uyanikgil Y, Gurler T Abstract An ideal anastomosis method will obtain the highest post-anastomotic vessel patency and will repair the vessel anatomically with minimal thrombosis in an easier, faster and cheaper fashion. To achieve these goals an anastomosis model using an amniotic membrane is introduced. The study was performed on the femoral arteries of 22 Wistar Albino rats (11 control group, 11 experimental group). In the experiment group, the microvascular anastomosis was completed with three sutures and a patch of amniotic membrane which was wrapp...
Source: Journal of Plastic Surgery and Hand Surgery - Category: Surgery Tags: J Plast Surg Hand Surg Source Type: research
Authors: Hurst Z, Liyanarachchi S, He H, Brock P, Sipos JA, Nabhan F, Kebebew E, Green P, Cote GJ, Sherman SI, Walker CJ, Chang YS, Xue S, Hollingsworth B, Li W, Genutis L, Menq E, de la Chapelle A, Jhiang SM Abstract BACKGROUND: Thyroid cancer patients with radioiodine refractory (RAI-R) disease, resulting from insufficient RAI delivery and/or RAI resistance, have increased mortality and limited treatment options. To date, studies have largely focused on tumor mutations associated with different stages of disease, which could provide prognostic value for RAI-R disease. We hypothesize that germline variants contrib...
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Authors: Mafficini A, Scarpa A Abstract Gastroenteropancreatic Neuroendocrine Neoplasms (GEP-NENs) are heterogeneous regarding site of origin, biological behavior and malignant potential. There has been a rapid increase in data publication over the last 10 years, mainly driven by high-throughput studies on pancreatic and small intestinal NETs. This review summarizes the present knowledge on genetic and epigenetic alterations. We integrated the available information from each compartment to give a pathway-based overview. This provided a summary of the critical alterations sustaining neoplastic cells. It also highlig...
Source: Endocrine Reviews - Category: Endocrinology Tags: Endocr Rev Source Type: research
ini Epithelial ovarian cancer (EOC) is the most lethal gynecological cancer. It is initially responsive to cisplatin and carboplatin, two DNA damaging agents used in first line therapy. However, almost invariably, patients relapse with a tumor resistant to subsequent treatment with platinum containing drugs. Several mechanisms associated with the development of acquired drug resistance have been reported. Here we focused our attention on DNA repair mechanisms, which are fundamental for recognition and removal of platinum adducts and hence for the ability of these drugs to exert their activity. We analyzed the major DNA...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
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