Effects of the stimuli-dependent enrichment of 8-oxoguanine DNA glycosylase1 on chromatinized DNA

Publication date: September 2018Source: Redox Biology, Volume 18Author(s): Wenjing Hao, Tianyang Qi, Lang Pan, Ruoxi Wang, Bing Zhu, Leopoldo Aguilera-Aguirre, Zsolt Radak, Tapas K. Hazra, Spiros A. Vlahopoulos, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan BoldoghAbstract8-Oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an epigenetic mark and that consequently OGG1 acquire distinct roles in modulation of gene expression. In support, lack of functional OGG1 in Ogg1-/- mice led to an altered expression of genes including those responsible for the aberrant innate and adaptive immune responses and susceptibility to metabolic disorders. Therefore, the present study examined stimulus-driven OGG1-DNA interactions at whole genome level using chromatin immunoprecipitation (ChIP)-coupled sequencing, and the roles of OGG1 enriched on the genome were validated by molecular and system-level approaches. Results showed that signaling levels of cellular ROS generated by TNFα, induced enrichment of OGG1 at specific sites of chromatinized DNA, primarily in the regulatory regions of genes. OGG1-ChIP-ed genes are associated with important cellular and biological processes and OGG1 enrichment was limited to a time scale required for immediate cellular responses. Preventi...
Source: Redox Biology - Category: Biology Source Type: research

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  It takes a strong leader to admit when his time is up. Endologix CEO John McDermott is that type of leader, which he proved this week with the announcement that he will be stepping down as CEO of the struggling endovascular aneurysm repair (EVAR) company.     Endologix has had its fair share of hurdles over the past couple of years, but it seems like things might finally be looking up for the Irvine, CA-based company. In May, the company promoted John Onopchenko to the role of CEO. Prior to the promotion, Onopchenko was the company's chief operating officer, a p...
Source: MDDI - Category: Medical Devices Authors: Tags: Business Source Type: news
CONCLUSIONS: The result of this retrospective study allows us to say that the choice of the technique, in the presence of multiple risk factors, should be carefully assessed and discussed with the patient. A prospective study with a larger number of patients would allow a better definition of the risk determined by individual factors and the development of a score that could be used in practice to simplify the risk assessment KEY WORDS: Conversion to open surgery, Incisional hernia, Laparoscopy, Risk factors. PMID: 30007062 [PubMed - as supplied by publisher]
Source: Annali Italiani di Chirurgia - Category: Surgery Tags: Ann Ital Chir Source Type: research
The AHR represses nucleotide excision repair and apoptosis and contributes to UV-induced skin carcinogenesis, Published online: 16 July 2018; doi:10.1038/s41418-018-0160-1The AHR represses nucleotide excision repair and apoptosis and contributes to UV-induced skin carcinogenesis
Source: Cell Death and Differentiation - Category: Cytology Authors: Source Type: research
Publication date: Available online 20 March 2018Source: Materials TodayAuthor(s): Sean M. Bittner, Jason L. Guo, Anthony Melchiorri, Antonios G. MikosAbstractThe field of tissue engineering has produced new therapies for the repair of damaged tissues and organs, utilizing biomimetic scaffolds that mirror the mechanical and biological properties of host tissue. The emergence of three-dimensional printing (3DP) technologies has enabled the fabrication of highly complex scaffolds that offer a more accurate replication of native tissue properties and architecture than previously possible. Of strong interest to tissue engineers...
Source: Materials Today - Category: Materials Science Source Type: research
Conclusions: The AAA patients undergoing endovascular aneurysm repair showed relatively low short-term mortality. However, larger groups of patients with ruptured AAA are required in order to assess the outcomes in this sub-population. PMID: 30002758 [PubMed]
Source: Videosurgery and Other Miniinvasive Techniques - Category: Surgery Tags: Wideochir Inne Tech Maloinwazyjne Source Type: research
Authors: Wang J Abstract Introduction: Minimally invasive surgery is a widely used method for bone repair. Nano-hydroxyapatite (n-HA) is a non-toxic and harmless bone repair material. Aim: To study the repair effect of n-HA/PA66 on the lumbar vertebrae injury of basketball players. Material and methods: Firstly, the n-HA/PA66 material was prepared and its properties were studied. Then, the n-HA/PA66 material was used in a rabbit vertebral replacement experiment to study its bone coverage, fiber coverage and bone density. Afterwards, the material was used in vertebral injury treatment of basketball players t...
Source: Videosurgery and Other Miniinvasive Techniques - Category: Surgery Tags: Wideochir Inne Tech Maloinwazyjne Source Type: research
CONCLUSION: Women with a previous bladder injury undergoing repeat cesarean delivery should have their bladder carefully inspected for any further urologic complications. PMID: 29999299 [PubMed - in process]
Source: Journal of Reproductive Medicine - Category: Reproduction Medicine Tags: J Reprod Med Source Type: research
CONCLUSION: Simple and effective cystotomy repair is possible even under extremely complex conditions. PMID: 29999297 [PubMed - in process]
Source: Journal of Reproductive Medicine - Category: Reproduction Medicine Tags: J Reprod Med Source Type: research
We presently forget 98% of everything we experience. That will go away in favor of perfect, controllable, configurable memory. Skills and knowledge will become commodities that can be purchased and installed. We will be able to feel exactly as we wish to feel at any given time. How we perceive the world will be mutable and subject to choice. How we think, the very fundamental basis of the mind, will also be mutable and subject to choice. We will merge with our machines, as Kurzweil puts it. The boundary between mind and computing device, between the individual and his or her tools, will blur. Over the course of the ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In this study, the model strain C. difficile 630 Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells for the duration tested. However, an extensive change in gene expression was determined by RNA-Seq with 49 effected genes after 15 min and more than 400 after one hour. Effected gene s exhibit a wide range of functionality, including the metabolism of amino acids, enzymes for cell wall synthesis, the uptake and metabolism of sugars, synthesis of B vitamins as well as purine synthesis and DNA repair. Our study suggests that tolerance of C. difficile towar...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Clostridioides difficile Source Type: research
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