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Total 40 results found since Jan 2013.

Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo.
Abstract We constructed a water-soluble lipopolymer (WSLP) as a nonviral gene carrier to deliver siRNA targeting NR2B. The cytotoxicity and serum stability of WSLP loaded with siRNA were evaluated, and the knockdown efficiency of WSLP/NR2B-siRNA in PC12 cells was examined. The results showed that WSLP could protect the loading siRNAs from enzymatic degradation in serum and exhibit low cytotoxicity to cells. After transfection, WSLP/NR2B-siRNA complexes reduced the NR2B transcriptional level by 50% and protein level by 55% compared to control siRNA. Moreover, 3 days after intrathecal injection of WSLP/NR2B-siRNA co...
Source: Pain Research and Management - April 8, 2018 Category: Anesthesiology Authors: Peng J, Ma J, Yang X, He H, Wu H, Ma T, Lu J Tags: Pain Res Manag Source Type: research

TRAF6 upregulation in spinal astrocytes maintains neuropathic pain by integrating TNF-α and IL-1β signaling
In this study, we investigated the role of TRAF6 in neuropathic pain in mice after spinal nerve ligation (SNL). SNL induced persistent TRAF6 upregulation in the spinal cord. Interestingly, TRAF6 was mainly colocalized with the astrocytic marker glial fibrillary acidic protein on SNL day 10 and partially expressed in microglia on SNL day 3. In cultured astrocytes, TRAF6 was upregulated after exposure to TNF-α or IL-1β. TNF-α or IL-1β also increased CCL2 expression, which was suppressed by both siRNA and shRNA targeting TRAF6. TRAF6 siRNA treatment also inhibited the phosphorylation of c-Jun N-terminal kinase (JNK) in as...
Source: Pain - September 29, 2014 Category: Anesthesiology Authors: Ying Lu, Bao-Chun Jiang, De-Li Cao, Zhi-Jun Zhang, Xin Zhang, Ru-Rong Ji, Yong-Jing Gao Tags: Research papers Source Type: research

Contribution of the Suppressor of Variegation 3-9 Homolog 1 in Dorsal Root Ganglia and Spinal Cord Dorsal Horn to Nerve Injury –induced Nociceptive Hypersensitivity
Conclusions The findings of this study suggest that SUV39H1 contributes to nerve injury –induced allodynia and hyperalgesia through gating MOR expression in the injured DRG. SUV39H1 may be a potential target for the therapeutic treatment of nerve injury–induced nociceptive hypersensitivity.
Source: Anesthesiology - September 20, 2016 Category: Anesthesiology Source Type: research

NLRP2 inflammasome in dorsal root ganglion as a novel molecular platform that produces inflammatory pain hypersensitivity
This study investigated the existence of the inflammasome in dorsal root ganglion (DRG) and the functional significance in the development of inflammatory pain hypersensitivity. Tissue inflammation was induced in male C57BL/6 mice with complete Freund's adjuvant (CFA) or ceramide injection into the hind paw. Behavioral testing was performed to investigate inflammation-induced pain hypersensitivity. Ipsilateral L5 DRGs were obtained for analysis. Expression of nucleotide oligomerization domain-like receptors (NLRs) was analyzed with real-time PCR. Cleaved interleukin (IL)-1β and NLRP2 expression was investigated with immun...
Source: Pain - August 21, 2019 Category: Anesthesiology Tags: Research Paper Source Type: research

CAV-1 Overexpression Exacerbates the Manifestation in EPAC-1-Induced Chronic Postsurgical Pain in Rats
CONCLUSION: CAV-1 mediates the functional coupling of microglia, astrocytes, and neurons, and thus EPAC-1/CAV-1 plays an important role in CPSP exacerbation.PMID:35958678 | PMC:PMC9357801 | DOI:10.1155/2022/8566840
Source: Pain Research and Management - August 12, 2022 Category: Anesthesiology Authors: Qian Hua Shiren Shen Yibin Qin Su Cao Source Type: research

P2X7 Receptor-Induced Bone Cancer Pain by Regulating Microglial Activity via NLRP3/IL-1beta Signaling
CONCLUSION: These findings suggest that targeting the microglial P2X7R activated NLRP3/IL-1beta signaling pathway could serve as a potential strategy for BCP treatment.PMID:36375190
Source: Pain Physician - November 14, 2022 Category: Anesthesiology Authors: Ping Wu Xiaoqi Wu Guohua Zhou Yin Wang Xiaojun Liu Run Lv YanSong Liu Qingping Wen Source Type: research

Reduction of SIRT1-Mediated Epigenetic Upregulation of Nav1.7 Contributes to Oxaliplatin-Induced Neuropathic Pain
CONCLUSIONS: These findings suggest that reduction of SIRT1-mediated epigenetic upregulation of Nav1.7 in the DRG contributes to the development of oxaliplatin-induced neuropathic pain in rats. The intrathecal drug delivery treatment of activating SIRT1 might be a novel therapeutic option for oxaliplatin-induced neuropathic pain.PMID:37192244
Source: Pain Physician - May 16, 2023 Category: Anesthesiology Authors: Ling-Jun Xu Jing Wang Gui-Dan Li Kai-Feng Shen Xing-Hui He Wei Wu Cui-Cui Liu Source Type: research

Unexpected association of the “inhibitory” neuroligin 2 with excitatory PSD95 in neuropathic pain
Summary: Modified expression and function of the adhesion molecule neuroligin 2 are involved in the excitation/inhibition imbalance leading to the dorsal horn network’s hyperexcitability in neuropathic rats.Abstract: In the spinal nerve ligation (SNL) model of neuropathic pain, synaptic plasticity shifts the excitation/inhibition balance toward excitation in the spinal dorsal horn. We investigated the deregulation of the synaptogenic neuroligin (NL) molecules, whose NL1 and NL2 isoforms are primarily encountered at excitatory and inhibitory synapses, respectively. In the dorsal horn of SNL rats, NL2 was overexpressed whe...
Source: Pain - July 29, 2013 Category: Anesthesiology Authors: Tiphaine Dolique, Alexandre Favereaux, Olivier Roca-Lapirot, Virginie Roques, Claire Léger, Marc Landry, Frédéric Nagy Tags: Research papers Source Type: research

NRG1‐ErbB signalling promotes microglia activation contributing to incision‐induced mechanical allodynia
ConclusionIncision‐induced NRG1 expression mediated activation of dorsal horn microglia and contributed to the development of mechanical allodynia. Specifically targeting NRG1‐ErbB signalling may therefore provide a new therapeutic intervention for relieving incision‐induced mechanical allodynia.
Source: European Journal of Pain - August 27, 2014 Category: Anesthesiology Authors: Y. Xiang, T. Liu, H. Yang, F. Gao, H. Xiang, A. Manyande, Y. Tian, X. Tian Tags: Original Article Source Type: research

Isoflurane but Not Sevoflurane or Desflurane Aggravates Injury to Neurons In Vitro and In Vivo via p75NTR-NF-ĸB Activation.
CONCLUSIONS:: Isoflurane but not sevoflurane or desflurane postexposure aggravates neurotoxicity in preinjured neurons via activation of p75 and NF-κB. These findings may have implications for the choice of volatile anesthetic being used in patients with or at risk for neuronal injury, specifically in patients with a stroke or history of stroke and in surgical procedures in which neuronal injury is likely to occur, such as cardiac surgery and neurovascular interventions. PMID: 25329094 [PubMed - as supplied by publisher]
Source: Anesthesia and Analgesia - October 17, 2014 Category: Anesthesiology Authors: Schallner N, Ulbrich F, Engelstaedter H, Biermann J, Auwaerter V, Loop T, Goebel U Tags: Anesth Analg Source Type: research

The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats.
CONCLUSIONS: The time courses of TNF-α, IL-6 and IL-1β mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury. PMID: 25844135 [PubMed]
Source: Korean Journal of Anesthesiology - November 18, 2015 Category: Anesthesiology Tags: Korean J Anesthesiol Source Type: research

Role of MnSOD in propofol protection of human umbilical vein endothelial cells injured by heat stress
Conclusion Propofol protected the heat stress-injured cells, at least partly, through upregulating MnSOD expression, effectively reducing the direct or indirect cell damage caused by oxidative stress.
Source: Journal of Anesthesia - January 13, 2016 Category: Anesthesiology Source Type: research

Positive feedback regulation between microRNA‐132 and CREB in spinal cord contributes to bone cancer pain in mice
ConclusionsThese findings suggest that activation of spinal CREB/CRTC1 signalling may play an important role in bone cancer pain. Interruption to the positive feedback regulation between CREB/CRTC1 and its target gene miR‐132 can effectively relieved the bone cancer‐induced mechanical allodynia and spontaneous pain. What does this study add?The positive feedback regulation between CREB/CRTC1 and its target gene miR‐132 in spinal cord plays an important role in bone cancer pain.
Source: European Journal of Pain - February 26, 2016 Category: Anesthesiology Authors: B. Hou, X. Cui, Y. Liu, W. Zhang, M. Liu, Y.E. Sun, Z. Ma, X. Gu Tags: Original Article Source Type: research