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Total 81 results found since Jan 2013.
TGFβ‐Responsive HMOX1 Expression is Associated with Stemness and Invasion in GBM
This article is protected by copyright. All rights reserved.
Targeted proteomic analyses revealed elevated expressions of transmembrane proteins HMOX1 and SLC16A1 on the surface of glioblastoma cancer stem cells (CSCs) relative to healthy neural stem cells (NSCs). In the hypoxic region of the tumor, these proteins were found to be expressed among pseudopalisading glioma cells that also express known stem cell factors. From biological assays that are known to evaluate stemness, HMOX1 expression was found to be associated with stemness that could be regulated through TGFβ and PTEN signaling networks. Additionally, siRNA me...
Source: Stem Cells - June 28, 2016 Category: Stem Cells Authors: Dhiman Ghosh, Ilya V. Ulasov, LiPing Chen, Karolina Wallenborg, Parvinder Hothi, Leroy Hood, Charles S. Cobbs Tags: Cancer Stem Cells Source Type: research
The PI3K/AKT/c ‐MYC Axis Promotes the Acquisition of Cancer Stem‐Like Features in Esophageal Squamous Cell Carcinoma
In conclusion, we have described a novel intratumoral heterogeneity in ESCC. The identification of the PI3K/AKT/c‐MYC axis as a driver of CSC‐like features carries therapeutic implications. Stem Cells 2016;34:2040–2051
Oxidative stress induced by H2O2 promotes RU/RR conversion and the acquisition of CSC‐like properties. (A) Reactive oxygen species (ROS) induced by H2O2 (500 μM for 24 hours) was measured by flow cytometry. (B) H2O2‐induced RU/RR conversion in KYSE150‐RU cells, as indicated by the increase of GFP‐positive cells and luciferase activity. Representative flow cytometry data at Day 4 was shown in...
Source: Stem Cells - May 22, 2016 Category: Stem Cells Authors: Hai ‐Feng Zhang, Chengsheng Wu, Abdulraheem Alshareef, Nidhi Gupta, Qing Zhao, Xiu‐E Xu, Ji‐Wei Jiao, En‐Min Li, Li‐Yan Xu, Raymond Lai Tags: Cancer Stem Cells Source Type: research
A Multi-Lineage Screen Reveals mTORC1 Inhibition Enhances Human Pluripotent Stem Cell Mesendoderm and Blood Progenitor Production
Publication date: Available online 28 April 2016 Source:Stem Cell Reports Author(s): Emanuel Joseph Paul Nazareth, Nafees Rahman, Ting Yin, Peter William Zandstra Human pluripotent stem cells (hPSCs) exist in heterogeneous micro-environments with multiple subpopulations, convoluting fate-regulation analysis. We patterned hPSCs into engineered micro-environments and screened responses to 400 small-molecule kinase inhibitors, measuring yield and purity outputs of undifferentiated, neuroectoderm, mesendoderm, and extra-embryonic populations. Enrichment analysis revealed mammalian target of rapamycin (mTOR) inhibition...
Source: Stem Cell Reports - April 28, 2016 Category: Stem Cells Source Type: research
Development of a cell-defined siRNA microarray for analysis of gene function in human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): Hi Chul Kim, Gi-Hwan Kim, Ssang-Goo Cho, Eun Ju Lee, Yong-Jun Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glas...
Source: Stem Cell Research - April 5, 2016 Category: Stem Cells Source Type: research
The PI3K/AKT/c‐MYC axis promotes the acquisition of cancer stem‐like features in esophageal squamous cell carcinoma
In conclusion, we have described a novel intra‐tumoral heterogeneity in ESCC. The identification of the PI3K/AKT/c‐MYC axis as a driver of CSC‐like features carries therapeutic implications. This article is protected by copyright. All rights reserved.
Oxidative stress induced by H2O2 promotes RU/RR conversion and the acquisition of CSC‐like properties. (A) Reactive oxygen species (ROS) induced by H2O2 (500μM for 24h) was measured by flow cytometry. (B) H2O2‐induced RU/RR conversion in KYSE150‐RU cells, as indicated by the increase of GFP‐positive cells and luciferase activity. Representative flow cytometry ...
Source: Stem Cells - March 31, 2016 Category: Stem Cells Authors: Hai‐Feng Zhang, Chengsheng Wu, Abdulraheem Alshareef, Nidhi Gupta, Qing Zhao, Xiu‐E Xu, Ji‐Wei Jiao, En‐Min Li, Li‐Yan Xu, Raymond Lai Tags: Cancer Stem Cells Source Type: research
Correction of Mutant p63 in EEC Syndrome Using siRNA Mediated Allele‐Specific Silencing Restores Defective Stem Cell Function
This study demonstrates the phenotypic correction of mutant stem cells (OMESCs) in EEC syndrome by means of siRNA mediated AS silencing with restoration of function. The application of siRNA, alone or in combination with cell‐based therapies, offers a therapeutic strategy for corneal blindness in EEC syndrome. Stem Cells 2016; 00:000—000
Source: Stem Cells - March 16, 2016 Category: Stem Cells Authors: Vanessa Barbaro, Annamaria A. Nasti, Claudia Vecchio, Stefano Ferrari, Angelo Migliorati, Paolo Raffa, Vincenzo Lariccia, Patrizia Nespeca, Mariangela Biasolo, Colin E. Willoughby, Diego Ponzin, Giorgio Palù, Cristina Parolin, Enzo Iorio Tags: Regenerative Medicine Source Type: research
A Disintegrin and Metalloproteinase Domain 17 Regulates Colorectal Cancer Stem Cells and Chemosensitivity Via Notch1 Signaling
The objective of the present study was to determine whether ADAM17 regulates the CSC phenotype in CRC and to elucidate the downstream signaling mechanism that mediates cancer stemness. We treated established CRC cell lines and a newly established human CRC cell line HCP-1 with ADAM17-specific small interfering RNA (siRNA) or the synthetic peptide inhibitor TAPI-2. The effects of ADAM17 inhibition on the CSC phenotype and chemosensitivity to 5-fluorouracil (5-FU) in CRC cells were examined. siRNA knockdown and TAPI-2 decreased the protein levels of cleaved Notch1 (Notch1 intracellular domain) and HES-1 in CRC cells. A decre...
Source: Stem Cells Translational Medicine - February 24, 2016 Category: Stem Cells Authors: Wang, R., Ye, X., Bhattacharya, R., Boulbes, D. R., Fan, F., Xia, L., Ellis, L. M. Tags: Cancer Stem Cells, Colon Stem Cells Source Type: research
Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): H.C. Kim, G.-H. Kim, S.-G. Cho, E.J. Lee, Y.-J. Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glass using human ...
Source: Stem Cell Research - February 20, 2016 Category: Stem Cells Source Type: research
Correction of mutant p63 in EEC syndrome using siRNA mediated allele specific silencing restores defective stem cell function
This study demonstrates the phenotypic correction of mutant stem cells (OMESCs) in EEC syndrome by means of siRNA mediated allele‐specific silencing with restoration of function. The application of siRNA, alone or in combination with cell‐based therapies, offers a therapeutic strategy for corneal blindness in EEC syndrome. This article is protected by copyright. All rights reserved.
Source: Stem Cells - February 17, 2016 Category: Stem Cells Authors: Vanessa Barbaro, Annamaria A. Nasti, Claudia Del Vecchio, Stefano Ferrari, Angelo Migliorati, Paolo Raffa, Vincenzo Lariccia, Patrizia Nespeca, Mariangela Biasolo, Colin E. Willoughby, Diego Ponzin, Giorgio Palù, Cristina Parolin, Enzo Di Iorio Tags: Regenerative Medicine Source Type: research
Peroxiredoxin II is essential for maintaining stemness by redox regulation in liver cancer cells
This article is protected by copyright. All rights reserved.
Source: Stem Cells - February 11, 2016 Category: Stem Cells Authors: Taeho Kwon, Yesol Bak, Young‐Ho Park, Gyu‐Beom Jang, Jeong‐Seok Nam, Jeong Eun Yoo, Young Nyun Park, In Seon Bak, Jin‐Man Kim, Do‐Young Yoon, Dae‐Yeul Yu Tags: Cancer Stem Cells Source Type: research
In human bone marrow stromal cells
Publication date: Available online 10 February 2016 Source:Stem Cell Research Author(s): H.C. Kim, G.-H. Kim, S.-G. Cho, E.J. Lee, Y.-J. Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glass using hum...
Source: Stem Cell Research - February 10, 2016 Category: Stem Cells Source Type: research
Systems genetics analysis of a recombinant inbred mouse cell culture panel reveals Wnt pathway member Lrp6 as a regulator of adult hippocampal precursor cell proliferation
This article is protected by copyright. All rights reserved.
Source: Stem Cells - February 3, 2016 Category: Stem Cells Authors: Suresh Kannan, Zeina Nicola, Rupert W. Overall, Muhammad Ichwan, Gerardo Ramírez‐Rodríguez, Anna Grzyb, Giannino Patone, Kathrin Saar, Norbert Hübner, Gerd Kempermann Tags: Tissue ‐Specific STEM Cells Source Type: research
Inhibition of Oct 3/4 mitigates the cardiac progenitor-derived myocardial repair in infarcted myocardium
Conclusion:
Our results indicate that CSCs-derived cardiac regeneration improves the restoration of cardiac function and is mediated through Oct 3/4.
Source: Stem Cell Research and Therapy - December 24, 2015 Category: Stem Cells Authors: Yu ZhaoJianfeng DuYoufang ChenYaoliang TangGangjian QinGuorong LvShougang ZhuangTing Zhao Source Type: research
Promotion of human mesenchymal stem cell osteogenesis by PI3-kinase/Akt signaling, and the influence of caveolin-1/cholesterol homeostasis
Conclusions:
PI3K/Akt signaling is a key pathway required for human MSC osteogenesis, and it is likely that localization of active Akt in non-caveolar and caveolar membrane rafts positively and negatively contributes to osteogenesis, respectively.
Source: Stem Cell Research and Therapy - December 1, 2015 Category: Stem Cells Authors: Natasha BakerJihee SohnRocky Tuan Source Type: research
Human umbilical cord‐derived mesenchymal stem cells elicit macrophages into an anti‐inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats
This article is protected by copyright. All rights reserved.
Source: Stem Cells - November 2, 2015 Category: Stem Cells Authors: Zongyan Xie, Haojie Hao, Chuan Tong, Yu Cheng, Jiejie Liu, Yaping Pang, Yiling Si, Yulin Guo, Li Zang, Yiming Mu, Weidong Han Tags: Original Research Source Type: research
