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Total 81 results found since Jan 2013.

Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): H.C. Kim, G.-H. Kim, S.-G. Cho, E.J. Lee, Y.-J. Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glass using human ...
Source: Stem Cell Research - February 20, 2016 Category: Stem Cells Source Type: research

Development of a cell-defined siRNA microarray for analysis of gene function in human bone marrow stromal cells
Publication date: March 2016 Source:Stem Cell Research, Volume 16, Issue 2 Author(s): Hi Chul Kim, Gi-Hwan Kim, Ssang-Goo Cho, Eun Ju Lee, Yong-Jun Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glas...
Source: Stem Cell Research - April 5, 2016 Category: Stem Cells Source Type: research

Nanocarrier mediated siRNA delivery targeting stem cell differentiation.
Abstract Stem cell based regenerative medicine holds exceptional therapeutic potential and hence the development of efficient techniques to enhance control over the rate of differentiation has been the focus of active research. One of the strategies to achieve this involves delivering siRNA into stem cells and exploiting the RNA interference (RNAi) mechanism. Transport of siRNA across the cell membrane is a challenge due to its anionic property, especially in primary human cells and stem cells. Moreover, naked siRNA incites immune responses, may cause off-target effects, exhibits low stability and is easily degrad...
Source: Current Stem Cell Research and Therapy - November 30, 2019 Category: Stem Cells Authors: Fernandes F, Kotharkar P, Chakravorty A, Kowshik M, Talukdar I Tags: Curr Stem Cell Res Ther Source Type: research

Correction of mutant p63 in EEC syndrome using siRNA mediated allele specific silencing restores defective stem cell function
This study demonstrates the phenotypic correction of mutant stem cells (OMESCs) in EEC syndrome by means of siRNA mediated allele‐specific silencing with restoration of function. The application of siRNA, alone or in combination with cell‐based therapies, offers a therapeutic strategy for corneal blindness in EEC syndrome. This article is protected by copyright. All rights reserved.
Source: Stem Cells - February 17, 2016 Category: Stem Cells Authors: Vanessa Barbaro, Annamaria A. Nasti, Claudia Del Vecchio, Stefano Ferrari, Angelo Migliorati, Paolo Raffa, Vincenzo Lariccia, Patrizia Nespeca, Mariangela Biasolo, Colin E. Willoughby, Diego Ponzin, Giorgio Palù, Cristina Parolin, Enzo Di Iorio Tags: Regenerative Medicine Source Type: research

Correction of Mutant p63 in EEC Syndrome Using siRNA Mediated Allele‐Specific Silencing Restores Defective Stem Cell Function
This study demonstrates the phenotypic correction of mutant stem cells (OMESCs) in EEC syndrome by means of siRNA mediated AS silencing with restoration of function. The application of siRNA, alone or in combination with cell‐based therapies, offers a therapeutic strategy for corneal blindness in EEC syndrome. Stem Cells 2016; 00:000—000
Source: Stem Cells - March 16, 2016 Category: Stem Cells Authors: Vanessa Barbaro, Annamaria A. Nasti, Claudia Vecchio, Stefano Ferrari, Angelo Migliorati, Paolo Raffa, Vincenzo Lariccia, Patrizia Nespeca, Mariangela Biasolo, Colin E. Willoughby, Diego Ponzin, Giorgio Palù, Cristina Parolin, Enzo Iorio Tags: Regenerative Medicine Source Type: research

In human bone marrow stromal cells
Publication date: Available online 10 February 2016 Source:Stem Cell Research Author(s): H.C. Kim, G.-H. Kim, S.-G. Cho, E.J. Lee, Y.-J. Kwon Small interfering RNA (siRNA) screening approaches have provided useful tools for the validation of genetic functions; however, image-based siRNA screening using multiwell plates requires large numbers of cells and time, which could be the barrier in application for gene mechanisms study using human adult cells. Therefore, we developed the advanced method with the cell-defined siRNA microarray (CDSM), for functional analysis of genes in small scale within slide glass using hum...
Source: Stem Cell Research - February 10, 2016 Category: Stem Cells Source Type: research

Promotion of human mesenchymal stem cell osteogenesis by PI3-kinase/Akt signaling, and the influence of caveolin-1/cholesterol homeostasis
Conclusions: PI3K/Akt signaling is a key pathway required for human MSC osteogenesis, and it is likely that localization of active Akt in non-caveolar and caveolar membrane rafts positively and negatively contributes to osteogenesis, respectively.
Source: Stem Cell Research and Therapy - December 1, 2015 Category: Stem Cells Authors: Natasha BakerJihee SohnRocky Tuan Source Type: research

Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages by Upregulating miR‐181a Level
Abstract The mechanism by which addictive drugs such as morphine regulate adult neurogenesis remains elusive. We now demonstrate that morphine can regulate neurogenesis by control of miR‐181a and subsequent hippocampal neural progenitor cell (hNPC) lineages. In the presence of morphine, hNPCs preferentially differentiated into astrocytes, an effect blocked by the specific μ‐opioid receptor antagonist, Cys2‐Tyr3‐Orn5‐Pen7‐amide. This effect was mediated by the Prox1/Notch1 pathway as demonstrated by an increase in Notch1 level in the morphine‐ but not fentanyl‐treated hNPCs and blocked by overexpression of ...
Source: Stem Cells - October 14, 2014 Category: Stem Cells Authors: Chi Xu, Yue Zhang, Hui Zheng, Horace H. Loh, Ping‐Yee Law Tags: Tissue‐Specific Stem Cells Source Type: research

P2X7 Receptors mediate Innate phagocytosis by Human Neural Precursor Cells and Neuroblasts
ABSTRACT During early human neurogenesis there is overproduction of neuroblasts and neurons accompanied by widespread programmed cell death (PCD). Whilst it is understood that CD68+ microglia and astrocytes mediate phagocytosis during target‐dependent PCD, little is known of the cell identity or the scavenger molecules utilized to remove apoptotic corpses during the earliest stages of human neurogenesis. Using a combination of multiple‐marker immunohistochemical staining, functional blocking antibodies and antagonists, we showed that human neural precursor cells (hNPCs) and neuroblasts express functional P2X7 receptors...
Source: Stem Cells - October 21, 2014 Category: Stem Cells Authors: Michael D. Lovelace, Ben J. Gu, Steven S. Eamegdool, Michael W. Weible, James S. Wiley, David G. Allen, Tailoi Chan‐Ling Tags: Tissue‐specific stem cells Source Type: research

Autotaxin‐LPA Axis regulates hMSC migration by adherent junction disruption and cytoskeletal rearrangement via LPAR1/3‐dependent PKC/GSK3β/ β‐catenin and PKC/Rho GTPase pathways
In conclusion, ATX/LPA stimulates the migration of hMSCs through LPAR 1/3‐dependent E‐cadherin reduction and cytoskeletal rearrangement via PKC/GSK3β/β‐catenin and PKC/Rho GTPase pathways. Stem Cells 2014
Source: Stem Cells - November 6, 2014 Category: Stem Cells Authors: Jung Min Ryu, Ho Jae Han Tags: Regenerative Medicine Source Type: research

Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines
Conclusions: Repression of ncRNAs derived from the DLK1-DIO3 imprinted locus is associated with reduced neural lineage differentiation potential in hESCs.
Source: Stem Cell Research and Therapy - January 5, 2015 Category: Stem Cells Authors: Chu-Fan MoFang-Chun WuKang-Yu TaiWei-Chun ChangKai-Wei ChangHung-Chi KuoHong-Nerng HoHsin-Fu ChenShau-Ping Lin Source Type: research

High throughput differentiation and screening of a library of mutant stem cell clones defines new host‐based genes involved in rabies virus infection
This article is protected by copyright. All rights reserved.
Source: Stem Cells - March 5, 2015 Category: Stem Cells Authors: Deeann Wallis, Kimberly Loesch, Stacy Galaviz, Qingan Sun, Michael DeJesus, Thomas Ioerger, James C. Sacchettini Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research

Systematic implantation of dedifferentiated fat cells ameliorated monoclonal antibody 1-22-3-induced glomerulonephritis by immunosuppression with increases in TNF-stimulated gene 6
Conclusion: Systematic implantation of DFAT cells effectively ameliorated mAb 1-22-3-induced glomerulonephritis through immunosuppressive effects accompanied by the suppression of macrophage infiltration and expression of IL-6, IL-10 and IL12β, and increased production of serum and renal TSG-6 that improved the mAb 1-22-3-induced renal degeneration by the immunosuppressive effects of TSG-6. Thus DFAT cells will be suitable cell source for the treatment of immunological progressive renal diseases.
Source: Stem Cell Research and Therapy - April 16, 2015 Category: Stem Cells Authors: Takashi MaruyamaNoboru FukudaTaro MatsumotoKoichiro KanoMorito EndoMinako KazamaTomohiko KazamaJin IkedaHiroyuki MatsudaTakahiro UenoMasanori AbeKazuyoshi OkadaMasayoshi SomaKoichi MatsumotoHiroshi Kawachi Source Type: research

Molecular differences between stromal cell populations from deciduous and permanent human teeth
Conclusions: Taken together, our data suggest that deciduous pulp cells are more robust in self- renewal and proliferation, whereas adult dental pulp cells are more capable of signaling and matrix synthesis.
Source: Stem Cell Research and Therapy - April 18, 2015 Category: Stem Cells Authors: Nina KaukuaMo ChenPaolo GuarnieriMarkus DahlMei LimTülay Yucel-LindbergErik SundströmIgor AdameykoJeremy MaoKaj Fried Source Type: research

High‐Throughput Differentiation and Screening of a Library of Mutant Stem Cell Clones Defines New Host‐Based Genes Involved in Rabies Virus Infection
Abstract We used a genomic library of mutant murine embryonic stem cells (ESCs) and report the methodology required to simultaneously culture, differentiate, and screen more than 3,200 heterozygous mutant clones to identify host‐based genes involved in both sensitivity and resistance to rabies virus infection. Established neuronal differentiation protocols were miniaturized such that many clones could be handled simultaneously, and molecular markers were used to show that the resultant cultures were pan‐neuronal. Next, we used a green fluorescent protein (GFP) labeled rabies virus to develop, validate, and implement on...
Source: Stem Cells - May 12, 2015 Category: Stem Cells Authors: Deeann Wallis, Kimberly Loesch, Stacy Galaviz, Qingan Sun, Michael DeJesus, Thomas Ioerger, James C. Sacchettini Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research