Filtered By:
Specialty: Virology

This page shows you your search results in order of relevance. This is page number 10.

Order by Relevance | Date

Total 371 results found since Jan 2013.

Protein Phosphatase-1 Regulates Rift Valley Fever Virus Replication.
Abstract Rift Valley fever virus (RVFV), genus Phlebovirus family Bunyaviridae, is an arthropod-borne virus endemic throughout sub-Saharan Africa. Recent outbreaks have resulted in cyclic epidemics with an increasing geographic footprint, devastating both livestock and human populations. Despite being recognized as an emerging threat, relatively little is known about the virulence mechanisms and host interactions of RVFV. To date there are no FDA approved therapeutics or vaccines for RVF and there is an urgent need for their development. The Ser/Thr protein phosphatase 1 (PP1) has previously been shown to play a s...
Source: Antiviral Research - January 19, 2016 Category: Virology Authors: Baer A, Shafagati N, Benedict A, Ammosova T, Ivanov A, Hakami RM, Terasaki K, Makino S, Nekhai S, Kehn-Hall K Tags: Antiviral Res Source Type: research

Phosphorylation of NS5A Serine-235 is essential to hepatitis C virus RNA replication and normal replication compartment formation.
Abstract Hepatitis C virus (HCV) NS5A protein is essential for HCV RNA replication and virus assembly. Here we report the identification of NS5A phosphorylation sites Ser-222, Ser-235 and Thr-348 during an infectious HCV replication cycle and demonstrate that Ser-235 phosphorylation is essential for HCV RNA replication. Confocal microscopy revealed that both phosphoablatant (S235A) and phosphomimetic (S235D) mutants redistribute NS5A to large juxta-nuclear foci that display altered colocalization with known replication complex components. Using electron microscopy (EM) we found that S235D alters virus-induced memb...
Source: Virology - February 10, 2016 Category: Virology Authors: Eyre NS, Hampton-Smith RJ, Aloia AL, Eddes JS, Simpson KJ, Hoffmann P, Beard MR Tags: Virology Source Type: research

Suppression of lytic replication of Kaposi’s sarcoma-associated herpesvirus by autophagy during initial infection in NIH 3T3 fibroblasts
Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) is the infectious cause of the angioproliferative neoplasm Kaposi’s sarcoma (KS). We first confirmed the susceptibility of NIH 3T3 fibroblasts to KSHV by infecting them with BCP-1-derived KSHV. Lytic replication of KSHV was confirmed by PCR amplification of viral DNA isolated from culture supernatants of KSHV-infected cells. The template from KSHV-infected NIH 3T3 cells resulted in an intense viral DNA PCR product. A time course experiment revealed the disappearance of KSHV-specific DNA in culture supernatant of NIH 3T3 cells during a period between 48 h...
Source: Archives of Virology - February 24, 2016 Category: Virology Source Type: research

Caveolin- and clathrin-independent entry of BKPyV into primary human proximal tubule epithelial cells.
Abstract BK polyomavirus (BKPyV) is a human pathogen that causes polyomavirus-associated nephropathy and hemorrhagic cystitis in transplant patients. Gangliosides and caveolin proteins have previously been reported to be required for BKPyV infection in animal cell models. Recent studies from our lab and others, however, have indicated that the identity of the cells used for infection studies can greatly influence the behavior of the virus. We therefore wished to re-examine BKPyV entry in a physiologically relevant primary cell culture model, human renal proximal tubule epithelial cells. Using siRNA knockdowns, we ...
Source: Virology - February 19, 2016 Category: Virology Authors: Zhao L, Marciano AT, Rivet CR, Imperiale MJ Tags: Virology Source Type: research

Tiger Frog Virus ORF080L Protein Interacts with LITAF and Impairs EGF-induced EGFR Degradation.
Abstract Tiger frog virus (TFV) belongs to the genus Ranavirus, family Iridoviridae, and causes severe mortality in commercial cultures in China. TFV ORF080L is a gene homolog of lipopolysaccharide-induced TNF-α factor (LITAF), which is a regulator in endosome-to-lysosome trafficking through its function in the endosomal sorting complex required for transport machinery. The characteristics and biological roles of TFV ORF080L were identified. TFV ORF080L was predicted to encode an 84-amino acid peptide (VP080L). It had high-sequence identity with mammalian LITAF, but lacked the N-terminus of LITAF, which contains ...
Source: Virus Research - March 5, 2016 Category: Virology Authors: Chen YS, Chen NN, Qin XW, Mi S, He J, Lin YF, Weng SP, Guo CJ, Heb JG Tags: Virus Res Source Type: research

Hemagglutinin of influenza A virus binds specifically to cell surface nucleolin and plays a role in virus internalization.
Abstract The hemagglutinin (HA) protein of influenza A virus initiates cell entry by binding to sialic acids on target cells. In the current study, we demonstrated that in addition to sialic acids, influenza A/Puerto Rico/8/34 H1N1 (PR8) virus HA specifically binds to cell surface nucleolin (NCL). The interaction between HA and NCL was initially revealed with virus overlay protein binding assay (VOPBA) and subsequently verified with co-immunoprecipitation. Importantly, inhibiting cell surface NCL with NCL antibody, blocking PR8 viruses with purified NCL protein, or depleting endogenous NCL with siRNA all substanti...
Source: Virology - April 12, 2016 Category: Virology Authors: Chan CM, Chu H, Zhang AJ, Leung LH, Sze KH, Kao RY, Chik KK, To KK, Chan JF, Chen H, Jin DY, Liu L, Yuen KY Tags: Virology Source Type: research

Viruses, Vol. 8, Pages 103: ESCRT Requirements for Murine Leukemia Virus Release
The Murine Leukemia Virus (MLV) is a gammaretrovirus that hijack host components of the endosomal sorting complex required for transport (ESCRT) for budding. To determine the minimal requirements for ESCRT factors in MLV viral and viral-like particles (VLP) release, an siRNA knockdown screen of ESCRT(-associated) proteins was performed in MLV-producing human cells. We found that MLV VLPs and virions primarily engage the ESCRT-I factor Tsg101 and marginally the ESCRT-associated adaptors Nedd4-1 and Alix to enter the ESCRT pathway. Conversely, the inactivation of ESCRT-II had no impact on VLP and virion egress. By analyzing ...
Source: Viruses - April 17, 2016 Category: Virology Authors: Christina BartuschReinhild Prange Tags: Article Source Type: research

Asialoglycoprotein receptor facilitates infection of PLC/PRF/5 cells by HEV through interaction with ORF2
This article is protected by copyright. All rights reserved
Source: Journal of Medical Virology - May 8, 2016 Category: Virology Authors: Li Zhang, Yabin Tian, Zhiheng Wen, Feng Zhang, Ying Qi, Weijin Huang, Heqiu Zhang, Youchun Wang Tags: Research Article Source Type: research

Equine schlafen 11 restricts the production of equine infectious anemia virus via a codon usage-dependent mechanism.
Abstract Human schlafen11 is a novel restriction factor for HIV-1 based on bias regarding relative synonymous codon usage (RSCU). Here, we report the cloning of equine schlafen11 (eSLFN11) and the characteristics of its role in restricting the production of equine infectious anemia virus (EIAV), a retrovirus similar to HIV-1. Overexpression of eSLFN11 inhibited EIAV replication, whereas knockdown of endogenous eSLFN11 by siRNA enhanced the release of EIAV from its principal target cell. Notably, although eSLFN11 significantly suppressed expression of viral Gag protein and EIAV release into the culture medium, the ...
Source: Virology - May 16, 2016 Category: Virology Authors: Lin YZ, Sun LK, Zhu DT, Hu Z, Wang XF, Du C, Wang YH, Wang XJ, Zhou JH Tags: Virology Source Type: research

Targeting the pseudorabies virus DNA polymerase processivity factor UL42 by RNA interference efficiently inhibits viral replication
This study provides a new clue for the design of an intervention strategy against herpesviruses by targeting their processivity factors.
Source: Antiviral Therapy - July 4, 2016 Category: Virology Source Type: research

Targeting the pseudorabies virus DNA polymerase processivity factor UL42 by RNA interference efficiently inhibits viral replication.
This study provides a new clue for the design of an intervention strategy against herpesviruses by targeting their processivity factors. PMID: 27387827 [PubMed - as supplied by publisher]
Source: Antiviral Research - July 3, 2016 Category: Virology Authors: Wang YP, Huang LP, Du WJ, Wei YW, Wu HL, Feng L, Liu CM Tags: Antiviral Res Source Type: research

Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells.
In this study, we firstly found that inhibition of Hsp90 by pretreatment of host cells with 17-AAG, a specific inhibitor of Hsp90, or blocking Hsp90α/Hsp90β with siRNA, resulted in significantly reduced viral replication in PK-15 cells. But inhibition of Hsp90 by 17-AAG did not affect PCV2 entry into the host cells. Meanwhile, over-expression of Hsp90α/Hsp90β enhanced PCV2 genome replication and virion production. In addition, Hsp90β was enriched in the nuclear zone in the cells infected with PCV2. But it did not interact with the viral Cap/Rep proteins. It suggested that Hsp90 is required for PCV2 production in PK-15...
Source: Virus Research - August 19, 2016 Category: Virology Authors: Liu J, Zhang X, Ma C, You J, Dong M, Yun S, Jiang P Tags: Virus Res Source Type: research

Porphyromonas gingivalis-mediated signaling through TLR4 mediates persistent HIV infection of primary macrophages.
Abstract Periodontal infections contribute to HIV-associated co-morbidities in the oral cavity and provide a model to interrogate the dysregulation of macrophage function, inflammatory disease progression, and HIV replication during co-infections. We investigated the effect of Porphyromonas gingivalis on the establishment of HIV infection in monocyte-derived macrophages. HIV replication in macrophages was significantly repressed in the presence of P. gingivalis. This diminished viral replication was due partly to a decrease in the expression of integrated HIV provirus. HIV repression depended upon signaling throug...
Source: Virology - September 14, 2016 Category: Virology Authors: Agosto LM, Hirnet JB, Michaels DH, Shaik-Dasthagirisaheb YB, Gibson FC, Viglianti G, Henderson AJ Tags: Virology Source Type: research

Modelling Ebola virus dynamics: Implications for therapy
Publication date: Available online 12 October 2016 Source:Antiviral Research Author(s): Alexey Martyushev, Shinji Nakaoka, Kei Sato, Takeshi Noda, Shingo Iwami Ebola virus (EBOV) causes a severe, often fatal Ebola virus disease (EVD), for which no approved antivirals exist. Recently, some promising anti-EBOV drugs, which are experimentally potent in animal models, have been developed. However, because the quantitative dynamics of EBOV replication in humans is uncertain, it remains unclear how much antiviral suppression of viral replication affects EVD outcome in patients. Here, we developed a novel mathematical model to q...
Source: Antiviral Therapy - October 12, 2016 Category: Virology Source Type: research

Modelling Ebola virus dynamics: Implications for therapy.
Abstract Ebola virus (EBOV) causes a severe, often fatal Ebola virus disease (EVD), for which no approved antivirals exist. Recently, some promising anti-EBOV drugs, which are experimentally potent in animal models, have been developed. However, because the quantitative dynamics of EBOV replication in humans is uncertain, it remains unclear how much antiviral suppression of viral replication affects EVD outcome in patients. Here, we developed a novel mathematical model to quantitatively analyse human viral load data obtained during the 2000/01 Uganda EBOV outbreak and evaluated the effects of different antivirals....
Source: Antiviral Research - October 11, 2016 Category: Virology Authors: Martyushev A, Nakaoka S, Sato K, Noda T, Iwami S Tags: Antiviral Res Source Type: research