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Total 77 results found since Jan 2013.
Biophysical characterization of siRNA-loaded Lipid Nanoparticles with different PEG content in an aqueous system
This study evaluated the influence of PEGylation and stress conditions on the stability of siRNA-loaded LNPs with different concentrations of PEG (0.5 mol%; 0.5% PEG-LNP and 1.0 mol%; 1.0% PEG-LNP) anchored to their surface. We applied end-over-end agitation, elevated temperature, and repeated freeze and thaw (F/T) cycles as physicochemical stressors of pH and ionic strength. Dynamic light scattering (DLS), flow imaging microscopy (FIM), and ionic-exchange chromatography (IEX) were to determine the degree of aggregation and change in siRNA content. The results indicate that 0.5% PEG-LNP resisted aggregation only at low pH ...
Source: European Journal of Pharmaceutics and Biopharmaceutics - July 29, 2023 Category: Drugs & Pharmacology Authors: Ki Hyun Kim Ripesh Bhujel Ravi Maharjan Jae Chul Lee Hun Soon Jung Hye Jeong Kim Nam Ah Kim Seong Hoon Jeong Source Type: research
Inhibiting eukaryotic initiation factor 5A (eIF5A) hypusination attenuated activation of the SIK2 (salt-inducible kinase 2)-p4E-BP1 pathway involved in ovarian cancer cell proliferation and migration
CONCLUSION: The depletion of eIF5AHyp by GC7 or eIF5A-targeting siRNA attenuated activation of the SIK2-p4EBP1 pathway. In that way, eIF5AHyp depletion reduces the migration, clonogenicity, and viability of ES2 ovarian cancer cells.PMID:37219665 | DOI:10.1007/s11033-023-08510-5
Source: Molecular Biology Reports - May 23, 2023 Category: Molecular Biology Authors: Grace Kelly Lee Hae-Yeong Kim Jong Hwan Park Source Type: research
Improving reactivity of naphthalimide-based GST probe by imparting TPP cation: Development and application for live cell imaging
Bioorg Med Chem Lett. 2022 Dec 19:129109. doi: 10.1016/j.bmcl.2022.129109. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) Glutathione S-transferases (GSTs); triphenylphosphonium (TPP); 4-bromo-1,8-naphthalimide (BrNaph); reduced glutathione (GSH); hexahistidine (6×His); internal ribosome entry site (IRES); Hanks' Balanced Salt Solution (HBSS); small interfering RNA (siRNA) are a superfamily of multifunctional enzymes comprising multiple classes and subtypes. This paper describes the synthesis and characterization of TPPBN-1, a naphthalimide derivative conjugated with a triphenylphosphonium (TPP) cation. W...
Source: Bioorganic and Medicinal Chemistry Letters - December 22, 2022 Category: Chemistry Authors: Yuuta Fujikawa Kenta Terakado Sayaka Nezu Kota Noritsugu Yuki Maemoto Akihiro Ito Hideshi Inoue Source Type: research
Abstracts of Presentations at the Association of Clinical Scientists 143 < sup > rd < /sup > Meeting Louisville, KY May 11-14,2022
Conclusion: These assays are suitable for routine diagnostic. The UltraFast NextGenPCR is the fastest with average time (30mins), followed by Agilent (2 hrs) and MassArray (6hrs). Upon completion of this activity, participants should be able to examine, measure and compare results from different assays for SARS detection, evaluate and diagnose accurately, as well as being able to plan, organize and recommend a diagnostic procedure for diagnostic laboratory. Key words: SARS-CoV-2, RNA extraction, RT-PCR, limit of detection, quantification cycle, COVID-19, in vitro diagnostic tests, Agilent, Massarray, Ultrafast. [20] From t...
Source: Annals of Clinical and Laboratory Science - July 1, 2022 Category: Laboratory Medicine Source Type: research
