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Source: Oncology Reports
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Total 403 results found since Jan 2013.
Potential biomarkers for the therapeutic efficacy of sorafenib, sunitinib and everolimus.
We examined extracellular signal‑regulated kinase (ERK), 4E‑binding protein 1 (4EBP1) and p70 ribosomal S6 kinase (p70) as potential biomarkers for pretreatment prediction of the prognosis of patients with metastatic renal cell carcinoma (RCC) treated with sorafenib, sunitinib or everolimus. 786‑O and 769‑P cells were treated with sorafenib, sunitinib and everolimus. The expression of phosphorylated/total ERK, phosphorylated/total 4EBP1 and phosphorylated/total p70 was evaluated using western blotting. ERK, 4EBP1 and p70 were knocked down by siRNA in 786‑O and 769‑P cells. Then, the viability after treatment ...
Source: Oncology Reports - November 16, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
MET/ERK2 pathway regulates the motility of human alveolar rhabdomyosarcoma cells.
In this study, we demonstrated the functions of MET signaling in ARMS in vitro by using three human ARMS cell lines and three human embryonal rhabdomyosarcoma (ERMS) cell lines. MET mRNA levels and MET protein expression in ARMS cell lines was higher than those in ERMS cell lines as detected by real-time quantitative PCR and western blotting, respectively. Based on cell growth and cell cycle analyses it was found that HGF stimulation did not enhance the proliferation of ERMS or ARMS cell lines. HGF-stimulated cell motility of ARMS cell lines was inhibited by U0126 (ERK1/2 inhibitor) but was only partially inhibited by PD9...
Source: Oncology Reports - November 16, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Tumor-suppressive role of HACE1 in hepatocellular carcinoma and its clinical significance.
In conclusion, as a tumor suppressor, HACE1 may be a valuable prognostic biomarker and potential therapeutic target for HCC treatment.
PMID: 27805249 [PubMed - as supplied by publisher]
Source: Oncology Reports - November 3, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Dysregulation of micro-143-3p and BALBP1 contributes to the pathogenesis of the development of ovarian carcinoma.
The objective of the present study was to identify the association between mir-143-3p and RalA-binding protein 1 (RALBP1), and their roles in regulating the development of ovarian cancer. Overexpression of RALBP1 induced apoptosis of the ovarian cancer cells, and developed ovarian cancer. In silico analysis and luciferase assay were used to identify whether RALBP1 was the target of mir-143-3p. Subsequently, real‑time PCR and western blotting were used to determine the expression level of mir-143-3p, RALBP1 mRNA and protein in different groups, furthermore, MTT assay and flow cytometry were used to detect the viability ...
Source: Oncology Reports - October 18, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
High expression of fibronectin is associated with poor prognosis, cell proliferation and malignancy via the NF- κB/p53-apoptosis signaling pathway in colorectal cancer.
This study aimed to investigate fibronectin expression in colorectal cancer (CRC) and elucidate the effects of fibronectin on CRC by using a knockdown approach. Immunohistochemistry was used to evaluate the expression of fibronectin in 107 CRC patient tissues and gene expression was detected by real-time quantitative PCR (qPCR) and western blot analysis. Based on the above findings, the association among fibronectin expression, clinicopathological features and prognosis was analyzed. Next, fibronectin expression was silenced by small-interfering RNAs (siRNAs) and the effects of fibronectin siRNA transfection on CRC cells ...
Source: Oncology Reports - October 18, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
lncRNA-SNHG7 promotes the proliferation, migration and invasion and inhibits apoptosis of lung cancer cells by enhancing the FAIM2 expression.
Authors: She K, Huang J, Zhou H, Huang T, Chen G, He J
Abstract
There is growing evidence that long non-coding RNAs (lncRNAs) are related to cancer development. In the present study, we found that the expression levels of lncRNA-SNHG7 mRNA and protein obviously increased in lung cancer tissues compared to adjacent non-cancerous tissues. Simultaneously, the expression levels of Fas apoptotic inhibitory molecule 2 (FAIM2) also increased in lung cancer tissues. In addition, lncRNA-SNHG7 was of positive relevance with FAIM2 in human lung cancer tissues. Silence of lncRNA‑SNHG7 by siRNA repressed the level of FAIM2 p...
Source: Oncology Reports - September 28, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Clinical significance of CD44 variant 9 expression as a prognostic indicator in bladder cancer.
We examined its role in invasion and as a biomarker for the basal muscle invasive molecular subtype showing worse prognosis, and for tumor progression in high risk (pT1/high grade) non‑muscle invasive bladder cancers (NMIBCs). CD44v9, cytokeratin 5/6 (CK5/6), and cytokeratin 20 (CK20) expression was evaluated by immunohistochemistry in 98 pathologically confirmed specimens (36 muscle and 62 high‑risk non‑muscle) and correlated to clinical outcome. In vitro analysis was performed using two human bladder cancer cell lines (HT1376 and 5637). The CD44v9 high‑expressing group exhibited significantly lower progr...
Source: Oncology Reports - September 8, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
UII/GPR14 is involved in NF- κB-mediated colonic inflammation in vivo and in vitro.
In conclusion, UII/GPR14 signaling was involved in the DSS-induced colonic inflammation and its inhibition may serve as a potential therapeutic target, which may be associated with the NF-κB signaling pathway.
PMID: 27600191 [PubMed - as supplied by publisher]
Source: Oncology Reports - September 8, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
MicroRNA-3666-induced suppression of SIRT7 inhibits the growth of non-small cell lung cancer cells.
This study investigated the biological role and underlying mechanism of SIRT7 in NSCLC. Results showed that SIRT7 was highly expressed in NSCLC cell lines, as detected by real-time quantitative polymerase chain reaction and western blot analysis. SIRT7 knockdown by small interfering RNA (siRNA) significantly inhibited the growth of NSCLC cells and induced their apoptosis. Bioinformatics algorithms indicated that SIRT7 was a putative target of microRNA-3666 (miR-3666). Dual-luciferase reporter assay demonstrated that miR-3666 could target the 3'-untranslated region of SIRT7. Western blot analysis revealed that miR-3666 coul...
Source: Oncology Reports - September 8, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Ultrasound-targeted microbubble destruction of calcium channel subunit α 1D siRNA inhibits breast cancer via G protein-coupled receptor 30.
In conclusion, E2 upregulated the expression of Cav1.3 for Ca2+ influx to promote the expression of p-ERK1/2 for cell proliferation. The study confirmed that the mechanism of E2 inducing the expression of Cav1.3 through a non-genomic pathway, and highlighted that UTMD of Cav1.3 siRNA is a powerful promising technology for breast cancer gene therapy.
PMID: 27572936 [PubMed - as supplied by publisher]
Source: Oncology Reports - September 1, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma.
Authors: Xu Z, Gong J, Wang C, Wang Y, Song Y, Xu W, Liu Z, Liu Y
Abstract
Previous studies indicated that phosphoglycerate mutase 1 (PGAM1) is involved in many cancer types and promotes breast cancer progression. However, the role of PGAM1 in glioma remains unclear. The present study aimed to investigate the association of PGAM1 expression with glioma grade and the role of PGAM1 in proliferation, apoptosis, migration and invasion of glioma cells. The mRNA and protein expression of PGAM1 was analysed in glioma tissues and normal brain tissues. The expression of PGAM1 was examined further by immunohistochemical anal...
Source: Oncology Reports - September 1, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
PRL-3 promotes the peritoneal metastasis of gastric cancer through the PI3K/Akt signaling pathway by regulating PTEN.
In conclusion, our results provide a bridge between PRL-3 and PTEN; PRL-3 decreased the expression of PTEN as well as increased the level of PTEN phosphorylation and inactivated it, consequently activating the PI3K/Akt signaling pathway, and upregulating MMP-2/MMP-9 expression to promote GC cell peritoneal metastasis.
PMID: 27572739 [PubMed - as supplied by publisher]
Source: Oncology Reports - September 1, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12.
This study aimed to investigate the role of HBx-induced miR-21 in the apoptosis of HCC cells. In the study, interleukin-12 (IL-12) was demonstrated as a direct target of miR-21 by dual‑luciferase report assay, and miR-21 was highly expressed in HCC cells (HepG2 and HepG2 2.2.15) compared to L02 cells, but IL-12 was weakly expressed as detected by real-time quantitative PCR (RT-qPCR). Furthermore, miR-21 mimics, inhibitor, HBx-targeted siRNA, and the HBx overexpression vector (pHBx) were used to observe the regulatory effects of HBx-induced miR-21 via IL-12, and cell apoptosis was assessed. The results showed that over...
Source: Oncology Reports - September 1, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Downregulation of DNA-PKcs suppresses P-gp expression via inhibition of the Akt/NF- κB pathway in CD133-positive osteosarcoma MG-63 cells.
In this study, CD133+ and CD133‑negative (CD133‑) MG‑63 cells were isolated by magnetic activated cell sorting (MACS). We verified that CD133+ MG‑63 cells were more resistant to cisplatin (CDDP) than CD133‑ MG‑63 cells. DNA‑dependent protein kinase catalytic subunit (DNA‑PKcs) and P‑glycoprotein (P‑gp) were expressed at higher levels in the CD133+ MG‑63 cells compared with those levels in the CD133‑ MG‑63 cells, whereas downregulation of DNA‑PKcs by small interfering RNA (siRNA) decreased chemoresistance to CDDP and P‑gp expression at the mRNA and protein levels in these cells. This indi...
Source: Oncology Reports - August 9, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Amyloid precursor protein cooperates with c-KIT mutation/overexpression to regulate cell apoptosis in AML1-ETO-positive leukemia via the PI3K/AKT signaling pathway.
In conclusion, APP may cooperate with c-KIT mutation/overexpression in the regulation of cell apoptosis but not proliferation in AE leukemia via the PI3K/AKT signaling pathway.
PMID: 27460334 [PubMed - as supplied by publisher]
Source: Oncology Reports - July 29, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
