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Source: European Respiratory Journal

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Total 164 results found since Jan 2013.

Inhibition of the effect of epidermal growth factor (EGF) on lung cancer cells. The use of plasmids encoding specific siRNA molecules
Conclusions: The inhibition of EGF system blockade with siRNA technique appears to be more effective strategy in cancer treatment in vitro, as compared to erlotinib, since this effect is probably EGFR gene mutation-independent. However, we have not definitively proved if it resulted in increased tumor cell immunogenicity.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Gawronski, M., Kopinski, P., Jankowski, M., Goede, A., Szpechcinski, A., Chorostowska, J. Tags: 11.1 Lung Cancer Source Type: research

PDK1/RSK1-dependent C/EBP{beta} activation is involved in thrombin-induced expression of orosomucoid 1-like 3 (ORMDL3) and IL-8/CXCL8 in human lung epithelial cells
In this study, we investigated that the role of phosphoinositide-dependent kinase 1 (PDK1), ribosomal S6 kinase 1 (RSK1), and CCAAT/enhancer-binding protein β (C/EBPβ) in thrombin-induced expression of ORMDL3 and IL-8/CXCL8 in human lung epithelial cells (A549). We found that treatment of cells with thrombin caused ORMDL3 mRNA and protein expression. Thrombin-induced IL-8/CXCL8-luciferase activity or IL-8/CXCL8 release was inhibited by ORMDL3 siRNA, BX-912 (a PDK1 inhibitor), and PDK1 siRNA. Moreover, thrombin-stimulated ORMDL3 expression was attenuated by BX-912, PDK1 siRNA, RSK1 siRNA, and C/EBPβ siRNA. Th...
Source: European Respiratory Journal - December 6, 2017 Category: Respiratory Medicine Authors: Chen, B.-C., Huang, M.-F. Tags: Airway Pharmacology and Treatment Source Type: research

Late-breaking abstract: EGFR specific single chain antibody deliver siRNAs restore gefitinib sensitivity in resistant NSCLC cells
To overcome TKIs resistance, we developed scFv and s-9R, two human single chain antibodies against EGFR(fig.1A). Here, we showed s-9R could deliver FAM-siRNA into EGFR positve NSCLC cells(fig.1B). A549, H1993 and H1975 were either transfected with KRAS, MET or EGFR siRNA or co-incubated with scFv/s-9R/ indicated siRNA mixtures, following treated with gefitinib. Compared with the control groups, the target genes' expression level were down-regulated in the s-9R/siRNA mixture groups detected by RT-qPCR and western blot(fig.1C and D). In vivo, s-9R/siRNA mixtures restored gefitinib sensitivity in TKIs resistance cells measure...
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Lu, Y., Liu, L., Zhang, R., Ren, X. Tags: 11.1 Lung Cancer Source Type: research

The effects of INTS12 knockdown on processing of snRNAs
Conclusions: INTS12 is likely to play an important role in HBEC function via snRNA processing: this may affect transcription globally.Funding: Medical Research Council1. Baillat et al. (2005) Cell 123 (2): 265–76.2. Obeidat et al. (2013) PLoSOne 18;8(9): e74630.
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Kheirallah, A. K., Hodge, E., Nelson, C., Sayers, I., Hall, I. Tags: 3.2 Airway Cell Biology and Immunopathology Source Type: research

LSC 2014 abstract - ROR{gamma} t-specific RNAi decreases allergic airway inflammation and airway hyperresponsiveness in a mouse model of neutrophilic asthma
Conclusion: Our data show that knockdown of ROR t by gene silencing improved airway inflammation in a mouse model of experimental asthma and may have therapeutic potential for the treatment of neutrophilic asthma.
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Webering, S., Lunding, L., Fehrenbach, H., Wegmann, M. Tags: 5.3 Allergy and Immunology Source Type: research

Human airway trypsin-like protease stimulates protease activated receptors and interactions of these receptors
Conclusions: These results indicate that HAT may attack and activate PAR1, PAR2 and PAR3 in HBECs. These PARs, particularly PAR2 and PAR3, are intimately associated with IL-8 synthesis in HBECs, by mutual interrelations.
Source: European Respiratory Journal - November 20, 2019 Category: Respiratory Medicine Authors: Miki, M., Yasuoka, S., Tsutsumi, R., Takeuchi, Y., Miki, K., Kitada, S. Tags: Airway cell biology and immunopathology Source Type: research

INTS12 knockdown leads to perturbation of genes and pathways of relevance to airway biology
Conclusions: INTS12 regulates pathways important in lung function through perturbation of genes belonging to e.g. collagen formation pathway. INTS12 is likely to control protein synthesis through effects on tRNA aminoacylation pathway.Funding: Medical Research Council.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Kheirallah, A. K., Sayers, I., Hall, I. Tags: 3.1 Molecular Pathology and Functional Genomics Source Type: research

LATE-BREAKING ABSTRACT: Inhibition of the ubiquitin-E3-ligase FBXW7 enhances steroid efficacy: New concept for treating steroid insensitivity
Conclusion: Reduction of FBXW7 caused a significant augmentation of functional GR and may have the potential to improve the efficacy of steroids.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Kaestle, M., Wyatt, D., Nicklin, P. Tags: 5.3 Allergy and Immunology Source Type: research

Ribosomal protein S3 gene silencing protects against experimental allergic asthma
We reported here for the first time that RPS3 gene silencing ameliorates experimental allergic asthma, probably via interruption of NF-B activity, confirming RPS3 a novel therapeutic target for the treatment of allergic airway inflammation.
Source: European Respiratory Journal - November 7, 2016 Category: Respiratory Medicine Authors: Dong, J., Liao, W., Peh, H. Y., Chan, T. K., Tan, W. S. D., Li, L., Wong, W. S. F. Tags: 5.3 Allergy and Immunology Source Type: research

Increased CTGF expression of circulating fibrocytes in asthmatic patients with severe OSA - the role of HIF-1a and HDAC7
Conclusion: Severe OSA related hypoxia increases CTGF expression in circulating fibrocytes via activation of HIF-1α that requires HDAC7 nuclear co-transprotation. HDAC7 may serve a therapeutic target for airway remodeling in asthmatics with severe OSA.
Source: European Respiratory Journal - November 19, 2018 Category: Respiratory Medicine Authors: Wang, T.-Y., Lo, Y.-L., Wang, C.-H., Kuo, H.-P. Tags: Airway Cell Biology and Immunopathology Source Type: research

Late-breaking abstract: Inhibition of RC kinase is a novel therapeutic approach for the treatment of COPD and IPF
RC kinase is a novel serine/threonine kinase, expressed predominantly by pulmonary macrophage and type II bronchial epithelial cells. Examination of BAL macrophage mRNA isolated from COPD and IPF patients showed increased expression of RC kinase compared to normal smokers and non-smoking patients. In vitro studies showed an upregulation of RC kinase upon cell stress, including oxidative stress. This correlated with an increase of NF-kb activity and IL-8 production that could be inhibited by RC kinase siRNA or small molecule antagonists. Biochemical analysis revealed that RC kinase is predominantly a nuclear protein and int...
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Boehme, S., Coelho, A., Hogaboam, C., Ly, T. W., Bacon, K., Athanacio, J. Tags: 5.1 Airway Pharmacology and Treatment Source Type: research

CREB/CRTC-mediated regulation of transcription is important for regulatory T cells
Conclusion: Our data point to an activation of CREB/CRTC-mediated transcription in naturally occurring as well as in in vitro induced Tregs. CREB knockdown reduced the formation of iTregs suggesting a possible role of this pathway in immune tolerance.
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Bartel, S., Kastle, M., Eickelberg, O., Krauss-Etschmann, S. Tags: 5.3 Allergy and Immunology Source Type: research

Expression and function of aquaporin 1 in hypoxia-induced pulmonary hypertension
Conclusion: Hx modulates the expression of Aqp1 in vivo and in vitro. Inhibition of Aqp1 through siRNA resulted in less migration and more apoptosis. These data suggest an important functional role of Aqp1 in the development of hypoxia-induced PH.
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Schuoler, C., Haider, T., Leuenberger, C., Gassmann, M., Kohler, M., Huber, L., Brock, M. Tags: 4.3 Pulmonary Circulation and Pulmonary Vascular Disease Source Type: research

Reactive sulfur species are involved in cigarette smoke-induced cellular senescence
Conclusions: The current study suggests that the reduction in reactive sulfer species might be related to CS-induced cellular senescence.
Source: European Respiratory Journal - November 20, 2019 Category: Respiratory Medicine Authors: Yamanaka, S., Ichikawa, T., Sugiura, H., Numakura, T., Sano, H., Yamada, M., Fujino, N., Tanaka, R., Kyogoku, Y., Akaike, T., Ichinose, M. Tags: Molecular pathology and funct. genomics Source Type: research

Late Breaking Abstract - DCLK1 mediates thrombin-induced IL-8/CXCL8 expression in lung epithelial cells
Airway neutrophilia has been known correlated with asthma severity. Thrombin plays a crucial role in asthma. Thrombin can induce IL-8/CXCL8 expression, a potent chemoattractant for neutrophils, in lung epithelial cells. Doublecortin-like kinase 1 (DCLK1) is a protein kinase that has been identified as a tumor stem cell marker in colon and pancreatic cancer. Here we investigated the role of DCLK1 in thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells. Thrombin-induced IL-8/CXCL8 release was attenuated by DCLK1 siRNA or LRKK2-IN-1 (DCLK1 inhibitor) in BEAS-2B and A549 cells. Moreover, thrombin-induced IL-8/...
Source: European Respiratory Journal - November 25, 2021 Category: Respiratory Medicine Authors: Yuliani, F. S., Chen, J.-Y., Cheng, W.-H., Wen, H.-C., Chen, B.-C., Lin, C.-H. Tags: Allergy and immunology Source Type: research