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Source: The International Journal of Biochemistry and Cell Biology

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Total 120 results found since Jan 2013.

Downregulation of METTL14 increases apoptosis and autophagy induced by cisplatin in pancreatic cancer cells.
Abstract Pancreatic cancer is a leading cause of cancer-related death worldwide. Cisplatin is an essential drug treating patients with BRCA1/2 or PALB2 mutations. Whether other genetic determinants of cisplatin sensitivity exist and their underlying mechanisms remain unclear. Immunohistochemistry was used to determine METTL14 expression in pancreatic cancer tissues and non-tumoural tissues. Cell proliferation was detected with CCK-8 assays. Apoptosis was analysed via Western blotting and flow cytometry, and autophagy was analysed via Western blotting and immunofluorescence. In this work, we found higher METTL14 ex...
Source: The International Journal of Biochemistry and Cell Biology - February 21, 2020 Category: Biochemistry Authors: Kong F, Liu X, Zhou Y, Hou X, He J, Li Q, Miao X, Yang L Tags: Int J Biochem Cell Biol Source Type: research

Angiotensin II inhibits osteogenic differentiation of isolated synoviocytes by increasing DKK-1 expression.
This study aims to investigate the effect of angiotensin II (Ang II) on osteogenic differentiation of synoviocytes and the underlying mechanism. Ang II was showed to inhibite osteogenic differentiation of synoviocytes, which was mitigated by a Dickkopf-1 (DKK-1) inhibitor. DKK-1 was upregulated by Ang II, which was weakened by the Ang II type 1 receptor (AT1R) blocker, reactive oxygen species (ROS) scavenger, and p38 inhibitor. Ang II increased the levels of AT1R, ROS, and NADPH oxidase (NOX), and the upregulations were mitigated by the AT1R blocker or NOX inhibitor. Furthermore, Ang II activated the p38 pathway, which was...
Source: The International Journal of Biochemistry and Cell Biology - January 30, 2020 Category: Biochemistry Authors: Zhang Y, Ding H, Song Q, Wang Z, Yuan W, Ren Y, Zhao Z, Wang C Tags: Int J Biochem Cell Biol Source Type: research

MicroRNA-141-3p affected proliferation, chemosensitivity, migration and invasion of colorectal cancer cells by targeting EGFR.
Abstract Colorectal cancer (CRC) is one of the most often diagnosed cancers globally. MicroRNAs are small RNA molecules that play essential roles in tumorigenesis and progression of CRC. Here we evaluated the effects of miR-141-3p on growth, cetuximab sensitivity, migration and invasion of CRC cells. We found that miR-141-3p negatively regulated the proliferation, migration and invasion in CRC cells. In addition, miR-141-3p enhanced the cetuximab sensitivity of CRC cells by EGFR suppression. Moreover, miR-141-3p improved cetuximab-induced apoptosis in CRC cells. Furthermore, miR-141-3p altered the expression of E-...
Source: The International Journal of Biochemistry and Cell Biology - November 4, 2019 Category: Biochemistry Authors: Xing Y, Jing H, Zhang Y, Suo J, Qian M Tags: Int J Biochem Cell Biol Source Type: research

Cbf α1 hinders autophagy by DSPP upregulation in odontoblast differentiation.
In this study, we found the stimulation with FGF2 in MDPC-23 cells resulted in the suppression of autophagy and promotion of cell differentiation. We demonstrated that both mRNA and protein levels of Cbfα1 and DSPP were elevated upon FGF2 stimulation coupled with the inhibition of autophagy in MDPC-23 cells. Further results revealed that overdose of Cbfα1 increased the expression of DSPP, and also suppressed autophagy in MDPC-23 cells. Using luciferase reporter assay and EMSA, we illustrated that Cbfα1 upregulated DSPP expression through binding to the specific sites 5'-TACCTCA (-3950 bp to -3944 bp) and 5'-ACCACA (-310...
Source: The International Journal of Biochemistry and Cell Biology - July 29, 2019 Category: Biochemistry Authors: Guo T, Cao G, Liu B, Li Z, Chen W Tags: Int J Biochem Cell Biol Source Type: research

Comprehensive circular RNA expression profiles and the tumor-suppressive function of circHIPK3 in ovarian cancer.
CONCLUSIONS: We identified the circRNA expression profile in ovarian cancer tissues and further verified the existence and expression of six randomly selected differentially expressed circRNAs. Besides, we also found that circHIPK3 is an important regulator of ovarian cancer progression. PMID: 31026505 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - April 22, 2019 Category: Biochemistry Authors: Teng F, Xu J, Zhang M, Liu S, Gu Y, Zhang M, Wang X, Ni J, Qian B, Shen R, Jia X Tags: Int J Biochem Cell Biol Source Type: research

Induction of phosphatase shatterproof 2 by evodiamine suppresses the proliferation and invasion of human cholangiocarcinoma.
In this study, we studied whether this traditional Chinese Medicine could serve as new potential therapeutic drugs to treat CCA. We discovered that evodiamine inhibited CCA cell proliferation and induced apoptosis. Moreover, evodiamine inhibited CCA cell migration and invasion. Mechanistically, our studies demonstrated that evodiamine inhibited the activation of IL-6 -induced STAT3 signaling activation, and the inhibitory effect was likely due to the upregulation of phosphatase shatterproof 2 (SHP-2), a negative feedback regulator of IL-6/STAT3. Blockage of SHP-2 through small interference RNA (siRNA) abolished the evodiam...
Source: The International Journal of Biochemistry and Cell Biology - January 22, 2019 Category: Biochemistry Authors: Zhu B, Zhao L, Liu Y, Jin Y, Feng J, Zhao F, Sun J, Geng R, Wei Y Tags: Int J Biochem Cell Biol Source Type: research

Inhibition of HSP90 β by ganetespib blocks the microglial signalling of evoked pro-inflammatory responses to heat shock.
Inhibition of HSP90β by ganetespib blocks the microglial signalling of evoked pro-inflammatory responses to heat shock. Int J Biochem Cell Biol. 2018 Nov 15;: Authors: He GL, Luo Z, Shen TT, Yang J, Li P, Luo X, Yang XS Abstract Although microglial reaction to heat shock is considered to be protective, heat shock is still a potential hazard caused by high temperatures. Recent studies indicate that the inhibition of the 90-kDa heat shock protein (HSP90) increasing the protective heat shock response and suppressing inflammatory signaling pathways in several diseases. Nevertheless, the effects of heat s...
Source: The International Journal of Biochemistry and Cell Biology - November 15, 2018 Category: Biochemistry Authors: He GL, Luo Z, Shen TT, Yang J, Li P, Luo X, Yang XS Tags: Int J Biochem Cell Biol Source Type: research

Endothelial Replicative Senescence Delayed by the Inhibition of MTORC1 Signaling Involves MicroRNA-107.
This study sought to identify miRNAs involved in MTORC1-mediated inhibition of replicative senescence in endothelial cells. Pre-senescent HUVECs were prolonged treated with low dose rapamycin (1 nM), an MTOR inhibitor. Rapamycin treatment down-regulated the phosphorylated MTOR, RPS6 and 4EBP1 expressions, which confirmed MTORC1 suppression. Prolonged low dose rapamycin treatment has significantly reduced the percentage of senescence-associated beta galactosidase (SA-β gal) positively stained senescent cells and P16INK4 A expression in these cells. On the contrary, the percentage of BrdU-labelled proliferating cells ha...
Source: The International Journal of Biochemistry and Cell Biology - May 29, 2018 Category: Biochemistry Authors: Khor ES, Wong PF Tags: Int J Biochem Cell Biol Source Type: research

Inhibitory effect of microRNA-154 targeting WHSC1 on cell proliferation of human skin squamous cell carcinoma through mediating the P53 signaling pathway.
CONCLUSION: These results demonstrate that up-regulation of miR-154 inhibits proliferation and induces apoptosis of human skin SCC cells by down-regulating WHSC1 and blocking the P53 signaling pathway. PMID: 29727714 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - May 1, 2018 Category: Biochemistry Authors: Chen HQ, Gao D Tags: Int J Biochem Cell Biol Source Type: research

Suppressing Ku70/Ku80 expression elevates homology-directed repair efficiency in primary fibroblasts.
In this study, we used siRNA to suppress the expression of Ku70 and Ku80, which are the key factors in NHEJ pathway, to investigate the effect of Ku silencing on the HDR efficiency in pig fetal fibroblasts. Down-regulation of Ku70 and Ku80 resulted in the promotion of the frequencies of multiple HDR pathways, including homologous recombination, single strand annealing, and single-stranded oligonucleotide-mediated DNA repair. We further evaluated the effects of Ku70 and Ku80 silencing on promoting HR-mediated knock-in efficiency in two porcine endogenous genes and found a significant increase in the amount of knock-in cells...
Source: The International Journal of Biochemistry and Cell Biology - April 12, 2018 Category: Biochemistry Authors: Li G, Liu D, Zhang X, Quan R, Zhong C, Mo J, Huang Y, Wang H, Ruan X, Xu Z, Zheng E, Gu T, Hong L, Li Z, Wu Z, Yang H Tags: Int J Biochem Cell Biol Source Type: research

XBP1 activation enhances MANF expression via binding to endoplasmic reticulum stress response elements within MANF promoter region in hepatitis B.
Abstract As an endoplasmic reticulum (ER) stress-related protein, mesencephalic astrocyte-derived neurotrophic factor (MANF) is involved in inflammatory diseases, such as rheumatoid arthritis. However, the mechanisms of the transcriptional regulation of MANF is still undefined. Here, we showed that MANF expression was upregulated in hepatitis B tissues and hepatoma cells, and positively correlated with the spliced X-box binding protein-1 (XBP1s). Both overexpression of XBP1s and tunicamycin treatment were able to enhance MANF transcription. On the contrary, inhibition of XBP1 splicing by IRE1α endonuclease inhibi...
Source: The International Journal of Biochemistry and Cell Biology - April 9, 2018 Category: Biochemistry Authors: Wang D, Hou C, Cao Y, Cheng Q, Zhang L, Li H, Feng L, Shen Y Tags: Int J Biochem Cell Biol Source Type: research

Cyclic Biaxial Tensile Strain Promotes Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Cardiomyocyte-like Cells by miRNA-27a.
Abstract A physical stimuli, it has been reported that cyclic tensile strain can promote bone marrow-derived mesenchymal stem cells (BMSCs) to differentiate into cardiomyocytes, but the underlying mechanisms have been poorly elucidated so far. Here, we used a mimicking loading strain, cyclic biaxial tensile strain (CBTS), and found it can promote BMSCs to differentiate into cardiomyocytes. When the CBTS were loaded, the cells expressed cardiac-specific markers GATA4, TNNT2, MEF-2c, and Cx43, meanwhile we found miR-27a decreased and stem cell factor (SCF) increased. When we overexpressed miR-27a, the cardiac-specif...
Source: The International Journal of Biochemistry and Cell Biology - April 5, 2018 Category: Biochemistry Authors: Cao C, Li L, Li H, He X, Geng W, Xiaoqin Y Tags: Int J Biochem Cell Biol Source Type: research

mROS-TXNIP axis activates NLRP3 inflammasome to mediate renal injury during ischemic AKI.
Abstract Ischemia/reperfusion (I/R) is a critical risk factor for acute kidney injury (AKI). Recent studies provided evidence that tubular epithelial cells (TEC)-associated inflammation aggravates kidney injury and impairs tissue repair after I/R injury. Here we demonstrated that the Nod-like receptor protein 3 (NLRP3) inflammasome is activated by mitochondrial reactive oxygen species (mROS) during I/R injury via direct interactions between the inflammasome and thioredoxin-interacting protein (TXNIP). Firstly, we found that NLRP3 inflammasome activation was induced by I/R injury, peaking at day 3 after reperfusion...
Source: The International Journal of Biochemistry and Cell Biology - February 22, 2018 Category: Biochemistry Authors: Wen Y, Liu YR, Tang TT, Pan MM, Xu SC, Ma KL, Lv LL, Liu H, Liu BC Tags: Int J Biochem Cell Biol Source Type: research

Two-pore channels mediated receptor-operated Ca2+ entry in pulmonary artery smooth muscle cells in response to hypoxia.
In conclusion, the TPCs influence on function of pulmonary artery smooth muscle cells by mediated Ca2+ Signals under hypoxia condition. PMID: 29355755 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - January 17, 2018 Category: Biochemistry Authors: Jiang Y, Zhou Y, Peng G, Tian H, Pan D, Liu L, Yang X, Li C, Li W, Chen L, Ran P, Dai A Tags: Int J Biochem Cell Biol Source Type: research

Gelatin promotes cell aggregation and pro-inflammatory cytokine production in PMA-stimulated U937 cells by augmenting endocytosis-autophagy pathway.
Abstract Gelatin, denatured collagen, temporarily exists in tissues and may well be pathophysiologically involved in tissue remodeling, inflammation or tissue damage. The present study is aimed to investigate possible biological roles of gelatin by examining its effects on monocyte-like histiocytic lymphoma cell line U937. Once stimulated by phorbol 12-myristate 13-acetate (PMA), U937 cells differentiate into macrophage-like cells, changing from non-adherent to adherent cells with extended pseudopodia. Here we pre-treated the cell dishes with gelatin solution for cell culture. Interestingly, we found that PMA-stim...
Source: The International Journal of Biochemistry and Cell Biology - January 4, 2018 Category: Biochemistry Authors: Zhao YL, Lu ZY, Zhang X, Liu WW, Yao GD, Liu XL, Liu W, Wu QJ, Hayashi T, Yamato M, Fujisaki H, Hattori S, Atsuzawa Y, Tashiro SI, Onodera S, Ikejima T Tags: Int J Biochem Cell Biol Source Type: research