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Source: The International Journal of Biochemistry and Cell Biology

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Total 120 results found since Jan 2013.

Tyro3-mediated phosphorylation of ACTN4 at tyrosines is FAK-dependent and decreases susceptibility to cleavage by m-Calpain.
In this study, we sought the molecular mechanism of Tyro3 effects avoiding endogenous background by overexpression of Tyro3 in fibroblasts that have negligible levels of Tyro3. This introduction triggers the tyrosyl-phosphorylation of ACTN4, a member of actin binding protein family involved in motility, a behavior critical for invasive progression, as shown by siRNA to Tyro3 limiting melanoma cell migration and invasion. Tyro3-mediated phosphorylation of ACTN4 required FAK activation at tyrosine 397 and the EGF receptor cascade, but not EGFR ligand binding. Using PCR-based mutagenesis, the sites of Tyro3-mediated ACTN4 pho...
Source: The International Journal of Biochemistry and Cell Biology - December 20, 2017 Category: Biochemistry Authors: Shao H, Wang A, Lauffenburger D, Wells A Tags: Int J Biochem Cell Biol Source Type: research

CBX4 exhibits oncogenic activities in breast cancer via Notch1 signaling.
Abstract Polycomb chromobox (CBX) proteins are involved in gene silencing to function as oncogenes or tumor suppressors through the polycomb repressive complex (PRC1). CBX4 has been implicated in the progression of human cancers, but its role and clinical significance in breast cancer remain unclear. Here, we show that CBX4 is up-regulated in breast cancer and exerts oncogenic activities via miR-137-mediated activation of Notch1 signaling pathway. CBX4 expression was increased in breast cancer, compared with the nontumorous tissues. High CBX4 expression was closely correlated with tumor metastasis, advanced clinic...
Source: The International Journal of Biochemistry and Cell Biology - December 8, 2017 Category: Biochemistry Authors: Zeng JS, Zhang ZD, Pei L, Bai ZZ, Yang Y, Yang H, Tian QH Tags: Int J Biochem Cell Biol Source Type: research

Hypoxia-induced activation of specific members of the NF-kB family and its relevance to pulmonary vascular remodeling.
CONCLUSION: RelA in EC plays crucial role in hypoxia induced vascular remodeling and development of pH. Targeting RelA in EC alleviates SMC proliferation as well as inflammation related processes. PMID: 28987523 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - October 4, 2017 Category: Biochemistry Authors: Patel H, Zaghloul N, Lin K, Liu SF, Miller EJ, Ahmed M Tags: Int J Biochem Cell Biol Source Type: research

miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma.
Abstract Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) has been implicated in human cancers, but its role and clinical significance in hepatocellular carcinoma (HCC) remain unknown. Here, we show that HMGCS2 is downregulated and exhibits antimetastatic potential in HCC. Low expression of HMGCS2 was associated with poor tumor differentiation, vascular invasion and worse overall and disease-free survivals in two independent cohorts consisting of 743 cases. In vitro data demonstrated HMGCS2 overexpression suppressed, whereas HMGCS2 silence promoted HCC cell migration via Epithelial-Mesenchymal Transi...
Source: The International Journal of Biochemistry and Cell Biology - August 31, 2017 Category: Biochemistry Authors: Su SG, Yang M, Zhang MF, Peng QZ, Li MY, Liu LP, Bao SY Tags: Int J Biochem Cell Biol Source Type: research

Hypoxia Decrease Expression of Cartilage Oligomeric Matrix Protein to Promote Phenotype Switching of Pulmonary Arterial Smooth Muscle Cells.
Abstract Extracellular matrix proteins play important roles in the development of pulmonary hypertension(PH). However, the role of Cartilage oligomeric matrix protein (COMP) in the development of hypoxia-induced PH is largely unknown. We tested the hypothesis that COMP deficiency induced by hypoxia leads to the phenotype switching of pulmonary arterial smooth muscle cells (PASMCs). The expression of COMP decreased in a chronic hypoxia rat PH model (P<0.05) and in PASMCs under hypoxia (3%O2) (P< 0.05). The expressions of differentiated marker proteins reduced in the pulmonary arteries from 5 month old COMP(-/...
Source: The International Journal of Biochemistry and Cell Biology - August 28, 2017 Category: Biochemistry Authors: Yu H, Jia Q, Feng X, Chen H, Wang L, Ni X, Kong W Tags: Int J Biochem Cell Biol Source Type: research

LOX-1: A potential target for therapy in atherosclerosis.
Abstract Pro-inflammatory signal generated from the interaction of oxLDL with its cognate receptor LOX-1 has been attenuated successfully by a novel combination siRNA (siLOX-1(Ω)) targeting unique regions of Homo sapien LOX-1 mRNA. Signalling via LOX-1 was studied in a potentially pro-atherogenic arena recreated in a metabolic, pulse-chase set up. An initial pulse of oxLDL (20μg/mL;5h) was chased (without oxLDL) on a temporal scale upto 72h. Our study shows that the pro-inflammatory signal generated via oxLDL-LOX-1R interaction was mediated in two rungs, an initial sustained increase in LOX-1R expression up to ...
Source: The International Journal of Biochemistry and Cell Biology - August 28, 2017 Category: Biochemistry Authors: Arjuman A, Chandra NC Tags: Int J Biochem Cell Biol Source Type: research

The ω-carboxyl group of 7-ketocholesteryl-9-carboxynonanoate mediates the binding of oxLDL to CD36 receptor and enhances caveolin-1 expression in macrophages.
In conclusion, these observations suggest that oxLig-1 is a critical epitope of oxLDL that mediates the binding of oxLDL to CD36 and activates downstream Src-JNK/ERK1/2-NF-κB signal transduction, resulting in upregulation of caveolin-1 expression in macrophages. PMID: 28789920 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - August 5, 2017 Category: Biochemistry Authors: Li J, Yu C, Wang R, Xu J, Chi Y, Qin J, Liu Q Tags: Int J Biochem Cell Biol Source Type: research

Calreticulin regulates TGF- β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling.
In this study, we demonstrated in vitro that TGF-β1-induced EMT elevated the expression of CRT in A549 lung cancer cells. Subsequently, we confirmed that overexpression CRT had no capacity to induce A549 cells EMT alone, but successfully enhanced TGF-β1-induced-EMT. Furthermore, knockdown of CRT in A549 cells significantly suppressed changes of EMT marks expression induced by TGF-β1. On treatment with TGF-β1, overexpression of CRT could enhance the phosphorylation of both Smad2 and Smad3. Consistently, the knockdown of CRT by siRNA-CRT could inhibit Smad signaling pathway activated by TGF-β1. These results indicated t...
Source: The International Journal of Biochemistry and Cell Biology - August 1, 2017 Category: Biochemistry Authors: Wu Y, Xu X, Ma L, Yi Q, Sun W, Tang L Tags: Int J Biochem Cell Biol Source Type: research

YAP1 negatively regulates chondrocyte differentiation partly by activating the β-catenin signaling pathway.
Abstract YAP1 (Yes-associated protein 1) transcriptional coactivator is a downstream gene of the Hippo signaling pathway, which controls cell proliferation and differentiation. YAP1 plays a significant role in the regulation of cartilage and bone development. However, the molecular mechanism by which YAP1 regulates chondrocyte differentiation remains to be elucidated. Immunofluorescent staining was used to visualize the localization of YAP1 expression in the mouse chondroprogenitor ATDC5 cell line. ATDC5 cells with lentivirus-vector-mediated YAP1 overexpression and knockdown were established. The differentiation a...
Source: The International Journal of Biochemistry and Cell Biology - April 21, 2017 Category: Biochemistry Authors: Yang B, Sun H, Song F, Yu M, Wu Y, Wang J Tags: Int J Biochem Cell Biol Source Type: research

Sp1 is a Competitive Endogenous RNA of Klf4 during Odontoblast Differentiation.
In this study, we predicted a group of potential Klf4 ceRNAs with bioinformatics approach, and examined the expression of Klf4 and five interested potential ceRNAs including Sp1 using real-time PCR during odontoblastic differentiation of mDPC6T. The expression levels of both Sp1 and Klf4 were significantly upregulated during this process. In situ hybridization verified that Sp1 was co-expressed with Klf4 in the differentiating and the mature odontoblasts in vivo. Knockdown of Sp1 using siRNA resulted in a significant reduction of Klf4 and vice visa. This interaction was further confirmed to be miRNA dependent. Common miRNA...
Source: The International Journal of Biochemistry and Cell Biology - February 22, 2017 Category: Biochemistry Authors: Zhang J, Liu H, Lin H, Li S, Tao H, Zhang L, Yuan G, Chen Z Tags: Int J Biochem Cell Biol Source Type: research

Rev-erb Agonist and TGF- β Similarly Affect Autophagy but Differentially Regulate Hepatic Stellate Cell Fibrogenic Phenotype.
Rev-erb Agonist and TGF-β Similarly Affect Autophagy but Differentially Regulate Hepatic Stellate Cell Fibrogenic Phenotype. Int J Biochem Cell Biol. 2016 Nov 10;: Authors: Thomes PG, Brandon-Warner E, Li T, Donohue TM, Schrum LW Abstract We demonstrated that ligand-activated nuclear receptor Rev-erbα mitigates CCl4-induced liver fibrosis. Rev-erbα is also a novel regulator of autophagy, a crucial eukaryotic catabolic system in which lysosomes degrade substrates for energy generation. In hepatic stellate cells (HSC) autophagy is reportedly required for this purpose to activate HSCs during fibrogene...
Source: The International Journal of Biochemistry and Cell Biology - November 9, 2016 Category: Biochemistry Authors: Thomes PG, Brandon-Warner E, Li T, Donohue TM, Schrum LW Tags: Int J Biochem Cell Biol Source Type: research

UTX-1 regulates Tat-induced HIV-1 transactivation via changing the methylated status of histone H3.
Abstract Epigenetic modifications are thought to be important for gene expression changes during HIV-1 transcription and replication. The removal of histone H3 lysine27 (H3K27) trimethylation mark by UTX-1 is important for the robust induction of many specific genes during Tat-mediated HIV-1 transactvation. We found that UTX-1 enzymatic activity is needed for Tat to remove a repressive mark H3K27me3 in the HIV-1 long terminal repeat (LTR). UTX-1 converted the chromatin structure to a more transcriptionally active state by up-regulation of H3K4 methylation and down-regulation of H3K27 methylation on the specific re...
Source: The International Journal of Biochemistry and Cell Biology - September 22, 2016 Category: Biochemistry Authors: Zhang HS, Du GY, Liu Y, Zhang ZG, Zhou Z, Li H, Dai KQ, Yu XY, Gou XM Tags: Int J Biochem Cell Biol Source Type: research

Palmitic acid aggravates inflammation of pancreatic acinar cells by enhancing unfolded protein response induced CCAAT-enhancer-binding protein β-CCAAT-enhancer-binding protein α activation.
Abstract Hypertriglyceridemia is an independent risk factor for acute pancreatitis, in which the pathological mechanisms are not fully illustrated. Intracellular inflammatory response is a key pathological response in acute pancreatitis and endoplasmic reticulum stress has been suggested to induce inflammation and CCAAT-enhancer-binding protein expression. Therefore, the current study aims to elucidate the possible relationship between endoplasmic reticulum stress and inflammation in hypertriglyceridemia associated pancreatitis and the possible involvement of CCAAT-enhancer-binding protein. In cholecystokinin-8 st...
Source: The International Journal of Biochemistry and Cell Biology - August 30, 2016 Category: Biochemistry Authors: Wu J, Hu G, Lu Y, Zheng J, Chen J, Wang X, Zeng Y Tags: Int J Biochem Cell Biol Source Type: research

CD2-associated protein/phosphoinositide 3-kinase signaling has a preventive role in angiotensin II-induced podocyte apoptosis.
Abstract Angiotensin II (Ang II) works as a paracrine or autocrine cytokine agent to regulate renal functions and promotes podocytes dysfunction directly or indirectly, causing proteinuria. The glomerular slit diaphragm (SD) serves as a size-selective barrier and is linked to the actin-based cytoskeleton by adaptor proteins, including CD2-associated protein (CD2AP). Therefore, damages to CD2AP affect not only the function of the SD, but also directly disrupt the podocyte cytoskeleton, leading to proteinuria. In addition, CD2AP can facilitate the nephrin-induced phosphoinositide 3-kinase (PI3-K)/Akt signaling, whic...
Source: The International Journal of Biochemistry and Cell Biology - August 29, 2016 Category: Biochemistry Authors: Park HY, Seong SB, Min SY, Ha TS Tags: Int J Biochem Cell Biol Source Type: research

Decrease in acrolein toxicity based on the decline of polyamine oxidases.
Abstract We have shown recently that acrolein is strongly involved in cell damage during brain infarction and chronic renal failure. To study the mechanism of acrolein detoxification, we tried to isolate Neuro2a cells with reduced sensitivity to acrolein toxicity (Neuro2a-ATD cells). In one cell line, Neuro2a-ATD1, the level of glutathione (GSH) was increased. We recently isolated a second cell line, Neuro2a-ATD2, and found that acrolein-producing enzymes [polyamine oxidases (PAO); i.e. acetylpolyamine oxidase (AcPAO), and spermine oxidase (SMO)] are reduced in this cell line due to changes at the level of transcr...
Source: The International Journal of Biochemistry and Cell Biology - August 29, 2016 Category: Biochemistry Authors: Uemura T, Nakamura M, Sakamoto A, Suzuki T, Dohmae N, Terui Y, Tomitori H, Casero RA, Kashiwagi K, Igarashi K Tags: Int J Biochem Cell Biol Source Type: research