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Source: The International Journal of Biochemistry and Cell Biology

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Total 120 results found since Jan 2013.

Inhibiting post-translational core fucosylation prevents vascular calcification in the model of uremia.
In conclusion, this study provides evidence to suggest core fucosylation plays a major role in the process of VC and appropriate blockade of core fucosylation may represent a potential therapeutic strategy for treating VC in end-stage renal disease. PMID: 27521658 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - August 9, 2016 Category: Biochemistry Authors: Wen X, Liu A, Yu C, Wang L, Zhou M, Wang N, Fang M, Wang W, Lin H Tags: Int J Biochem Cell Biol Source Type: research

The mitochondrial calcium uniporter is involved in mitochondrial calcium cycle dysfunction: underlying mechanism of hypertension associated with mitochondrial tRNA(Ile) A4263G mutation.
In this study we found decreased MCU expression in hypertensive with mutation cells contributed to dysregulated Ca(2+) uptake into the mitochondria, and cytoplasmic Ca(2+) overload. This abnormality might be involved in the underlying mechanisms of maternally inherited hypertension in subjects carrying the mitochondrial tRNA(Ile) A4263G mutation. PMID: 27471128 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - July 24, 2016 Category: Biochemistry Authors: Chen X, Zhang Y, Xu B, Cai Z, Wang L, Tian J, Liu Y, Li Y Tags: Int J Biochem Cell Biol Source Type: research

Increased expression of platelet-derived growth factor associated protein-1 is associated with PDGF-B mediated glioma progression.
This study aimed to characterize the role of PDAP-1 in PDGF-mediated glioma proliferation. The expression of PDAP-1 was observed to be significantly increased (p<0.05) in grade IV glioma tissue and cell lines compared to grade III. siRNA-mediated knockdown of PDAP-1 reduced the expression of PDGF-B and its downstream genes (Akt1/Protein kinase B (PKB) and phosphoinositide-dependent kinase-1 (PDK1) by up to 50%. In PDAP-1 knockdown glioma cells, more than a twofold reduction was also observed in the level of phosphorylated Akt. Interestingly, knockdown of PDAP-1 in combination with PDGF-B antibody inhibited glioma cell p...
Source: The International Journal of Biochemistry and Cell Biology - July 18, 2016 Category: Biochemistry Authors: Sharma VK, Singh A, Srivastava SK, Kumar V, Gardi NL, Nalwa A, Dinda AK, Chattopadhyay P, Yadav S Tags: Int J Biochem Cell Biol Source Type: research

G9a inhibition induced PKM2 regulates autophagic responses.
Abstract Epigenetic regulation by histone methyltransferase G9a is known to control autophagic responses. As the link between autophagy and metabolic homeostasis is widely accepted, we investigated whether G9a affects metabolic circuitries to affect autophagic response in glioma cells. Both pharmacological inhibition and siRNA mediated knockdown of G9a increased autophagy marker LC3B in glioma cells. G9a inhibitor BIX-01294 (BIX) induced Akt-dependent increase in HIF-1α expression and activity. Inhibition of Akt-HIF-1α axis reversed BIX-mediated (i) increase in LC3B expression and (ii) decrease in Yes-associated...
Source: The International Journal of Biochemistry and Cell Biology - July 10, 2016 Category: Biochemistry Authors: Ahmad F, Dixit D, Joshi SD, Sen E Tags: Int J Biochem Cell Biol Source Type: research

Estrogen-related receptor (ERR) γ Protects against Puromycin Aminonucleoside-induced Podocyte Apoptosis by Targeting PI3K/Akt Signaling.
In this study, we investigated the role of estrogen-related receptor (ERR) γ in podocyte apoptosis, as well as the underlying mechanisms. Treatment of PAN caused a dose- and time-dependent podocyte apoptosis in line with a significant downregulation of ERRγ. Interestingly, the occurrence of ERRγ downregulation appeared earlier than the onset of cell apoptosis, suggesting a potential that ERRγ reduction triggered apoptotic response in podocyte. To test this hypothesis, ERRγ siRNA was administered to the podocytes. Strikingly, ERRγ silencing resulted in a significant cell apoptosis accompanied with increased injury mar...
Source: The International Journal of Biochemistry and Cell Biology - July 10, 2016 Category: Biochemistry Authors: Gong W, Yu J, Wang Q, Li S, Song J, Jia Z, Huang S, Zhang A Tags: Int J Biochem Cell Biol Source Type: research

Tumor Promoter PMA enhances kindlin-2 and decreases vimentin recruitment into cell adhesion sites.
Abstract Phorbol diester PMA (phorbol 12-myristate 13-acetate) is a well-known promoter of tumor progression. PMA also regulates cell adhesion by several mechanisms including conformational activation of integrins and integrin clustering. Here, PMA was shown to induce lamellipodia formation and reorganization of the adhesion sites as well as actin and vimentin filaments independently of integrin preactivation. To further analyze the mechanism of PMA action, the protein composition in the α1β1 integrin/collagen IV adhesion sites was analyzed by mass spectrometry and proteomics. In four independent experiments we ...
Source: The International Journal of Biochemistry and Cell Biology - June 28, 2016 Category: Biochemistry Authors: Salmela M, Rappu P, Lilja J, Niskanen H, Taipalus E, Jokinen J, Heino J Tags: Int J Biochem Cell Biol Source Type: research

Histone methyltransferase Suv39h1 attenuates high glucose-induced fibronectin and p21(WAF1) in mesangial cells.
Abstract Suppressor of variegation 3-9 homolog 1 (Suv39h1) is a histone methyltransferase that trimethylates lysine 9 of histone H3 (H3K9me3), which results in gene silencing. A previous study found that H3K9me3 and Suv39h1 were decreased in diabetic mouse vascular smooth muscle cells whereas Suv39h1 overexpression attenuated ischemic myocardial injury. Moreover, high glucose (HG) decreased H3K9me3 and Suv39h1 levels in some cells. Thus, we studied the roles of Suv39h1 in HG-induced effects in MES13 (mouse mesangial) cells. We found that HG, chaetocin (a Suv39h1 inhibitor) and Suv39h1 siRNA decreased Suv39h1 while...
Source: The International Journal of Biochemistry and Cell Biology - June 28, 2016 Category: Biochemistry Authors: Lin SH, Ho WT, Wang YT, Chuang CT, Chuang LY, Guh JY Tags: Int J Biochem Cell Biol Source Type: research

Tamoxifen-induced cytotoxicity in breast cancer cells is mediated by glucose-regulated protein 78 (GRP78) via AKT (Thr308) regulation.
Abstract Glucose regulated protein 78 (GRP78) has recently been suggested to be associated with drug resistance in breast cancer patients. However, the precise role of GRP78 in drug resistance and the involved signaling pathways are not clearly understood. In the present study, we show that among a panel of drugs, namely Paclitaxel (TAX), Doxorubicin (DOX), 5-fluorouracil (5-FU), UCN-01 and Tamoxifen (TAM) used, TAM alone up-regulated the expression of GRP78 significantly and induced apoptosis in MCF-7 and MDA-MB-231 cells. Interestingly, inhibition of GRP78 by a specific pharmacological inhibitor, VER-155008 augm...
Source: The International Journal of Biochemistry and Cell Biology - May 31, 2016 Category: Biochemistry Authors: Pujari R, Jose J, Bhavnani V, Kumar N, Shastry P, Pal JK Tags: Int J Biochem Cell Biol Source Type: research

Autophagy links β-catenin and Smad signaling to promote epithelial-mesenchymal transition via upregulation of integrin linked kinase.
Abstract TGF-β1 induces epithelial-mesenchymal transition (EMT) and autophagy in a variety of cells. However, the role of autophagy in TGF-β1-induced EMT has not been clearly elucidated and the underlying mechanisms are unclear. In the present study, we found that TGF-β1 induced both autophagy and EMT in mouse tubular epithelial C1.1 cells. Inhibition of autophagy by 3-methyladenine or siRNA knockdown of Beclin 1 reduced TGF-β1-induced increase of vimentin and decreased E-cadherin expression. In contrast, rapamycin-associated enhancement of TGF-β1-induced autophagy increased EMT of C1.1 cells. Serum rescue in...
Source: The International Journal of Biochemistry and Cell Biology - May 9, 2016 Category: Biochemistry Authors: Pang M, Wang H, Rao P, Zhao Y, Xie J, Cao Q, Wang Y, Wang YM, Lee VW, Alexander SI, Harris DC, Zheng G Tags: Int J Biochem Cell Biol Source Type: research

Janus kinases and Src family kinases in the regulation of EGF-induced vimentin expression in MDA-MB-468 breast cancer cells.
Abstract Epithelial-mesenchymal transition (EMT) is an important process associated with the metastasis of breast cancer cells. Members of the Janus kinases (JAKs) and Src family kinases (SFKs) are implicated in the regulation of an invasive phenotype in various cancer cell types. Using the pharmacological inhibitors JAK Inhibitor I (a pan-JAK inhibitor) and PP2 we investigated the role of the JAKs and SFKs, respectively, in the regulation of EMT markers in the MDA-MB-468 breast cancer cell line model of epidermal growth factor (EGF)-induced EMT. We identified selective inhibition of EGF induction of the mesenchym...
Source: The International Journal of Biochemistry and Cell Biology - May 5, 2016 Category: Biochemistry Authors: Stewart TA, Azimi I, Brooks AJ, Thompson EW, Roberts-Thomson SJ, Monteith GR Tags: Int J Biochem Cell Biol Source Type: research

Norisoboldine, an isoquinoline alkaloid, acts as an aryl hydrocarbon receptor ligand to induce intestinal Treg cells and thereby attenuate arthritis.
CONCLUSION: Oral NOR induces the generation of intestinal Treg cells by the activation of AhR, and thereby exerts anti-arthritic effect. PMID: 27032495 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - March 27, 2016 Category: Biochemistry Authors: Tong B, Yuan X, Dou Y, Wu X, Chou G, Wang Z, Xia Y, Daia Y Tags: Int J Biochem Cell Biol Source Type: research

Reactive oxygen species are involved in insulin-dependent regulation of autophagy in primary rat podocytes.
Abstract Autophagy is an intracellular defense mechanism responsible for the turnover of damaged or non-functional cellular constituents. This process provides cells with energy and essential compounds under unfavorable environmental conditions-such as oxidative stress and hyperglycemia, which are both observed in diabetes. The most common diabetes complication is diabetic nephropathy (DN), which can lead to renal failure. This condition often includes impaired podocyte function. Here we investigated autophagic activity in rat podocytes cultured with a high insulin concentration (300nM). Autophagy was activated af...
Source: The International Journal of Biochemistry and Cell Biology - March 25, 2016 Category: Biochemistry Authors: Audzeyenka I, Rogacka D, Piwkowska A, Rychlowski M, Bierla JB, Czarnowska E, Angielski S, Jankowski M Tags: Int J Biochem Cell Biol Source Type: research

Inhibitory effects of Omega-3 fatty acids on Early Brain Injury after Subarachnoid Hemorrhage in Rats: possible involvement of G protein-coupled receptor 120/β-arrestin2/TGF-β activated kinase-1 binding protein-1 signaling pathway.
This study was to examine the effects of omega-3 fatty acids on SAH-induced EBI. Two weeks before SAH, 30% Omega-3 fatty acids was administered by oral gavage at 1g/kg body weight once every 24hours. Specific siRNA for GPR120 was exploited. Terminal deoxynucleotidyl transferase dUTP nick end labeling, fluoro-Jade B staining, and neurobehavioral scores and brain water content test showed that omega-3 fatty acids effectively suppressed SAH-induced brain cell apoptosis and neuronal degradation, behavioral impairment, and brain edema. Western blot, immunoprecipitation, and electrophoretic mobility shift assays results showed t...
Source: The International Journal of Biochemistry and Cell Biology - March 17, 2016 Category: Biochemistry Authors: Yin J, Li H, Meng C, Chen D, Chen Z, Wang Y, Wang Z, Chen G Tags: Int J Biochem Cell Biol Source Type: research

Surface proteome analysis identifies platelet derived growth factor receptor-alpha as a critical mediator of transforming growth factor-beta-induced collagen secretion.
Abstract Fibroblasts are extracellular matrix-producing cells in the lung. Fibroblast activation by transforming growth factor-beta leads to myofibroblast-differentiation and increased extracellular matrix deposition, a hallmark of pulmonary fibrosis. While fibroblast function with respect to migration, invasion, and extracellular matrix deposition has been well-explored, little is known about the surface proteome of lung fibroblasts in general and its specific response to fibrogenic growth factors, in particular transforming growth factor-beta. We thus performed a cell-surface proteome analysis of primary human l...
Source: The International Journal of Biochemistry and Cell Biology - February 20, 2016 Category: Biochemistry Authors: Heinzelmann K, Noskovičová N, Merl-Pham J, Preissler G, Winter H, Lindner M, Hatz R, Hauck SM, Behr J, Eickelberg O Tags: Int J Biochem Cell Biol Source Type: research

TNF-α induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA in Kawasaki disease model.
In this study, firstly we verified the overexpressed TNF-α in KD patients, and found TNF-α was able to induce HUVECs apoptosis and inhibit HUVECs proliferation. After this we screened out pregnancy induced noncoding RNA (PINC) was significantly overexpression in TNF-α treated HUVECs. We also found PINC overexpressed in KD patients. For further study, we designed two siRNA of PINC. After silenced the expression of PINC in HUVECs, we found the Knockdown of PINC enhanced the viability of HUVECs treated with TNF-α, and increased the expression of anti-apoptotic and reduced the expression of apoptotic gene. These results su...
Source: The International Journal of Biochemistry and Cell Biology - January 18, 2016 Category: Biochemistry Authors: Jiang C, Fang X, Jiang Y, Shen F, Hu Z, Li X, Huang X Tags: Int J Biochem Cell Biol Source Type: research