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Analysis of the expression and association of retinoblastoma binding protein 6 (RBBP6) with the JNK signaling pathway in prostate cancers.
This study aims to investigate the expression of retinoblastoma binding protein 6 (RBBP6) in prostate cancer (PCa) and its association with the c-Jun N-terminal kinase (JNK) pathway. Immunohistochemistry was used to detect RBBP6 and JNK1/2 expression in PCa and benign prostatic hyperplasia (BPH) tissues. RBBP6 expression in PCa cells (LNCap, PC3 and DU145) and noncancerous prostate epithelial cells (RWPE-1) was determined by qRT-PCR and Western blotting. PC3 and DU145 cells were transfected with RBBP6 siRNAs to examine the biological characteristics. Anisomycin (a JNK activator) with/without RBBP6 siRNA was used to treat P...
Source: Cell Biology International - July 13, 2020 Category: Cytology Authors: Guan WY, Zhao S, Luo YN Tags: Cell Biol Int Source Type: research

URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion.
Abstract Upregulated gene 11 (URG11), a new gene upregulated by hepatitis B virus X protein, is involved in the development and progression of several tumors, including liver, stomach, lung, and colon cancers. However, the role of URG11 in prostate cancer remains yet to be elucidated. By determined expression in human prostate cancer tissues, URG11 was found significantly upregulated and positively correlated with the severity of prostate cancer, compared with that in benign prostatic hyperplasia tissues. Further, the mRNA and protein levels of URG11 were significantly upregulated in human prostate cancer cell lin...
Source: Biomed Res - June 30, 2018 Category: Research Authors: Pan B, Ye Y, Liu H, Zhen J, Zhou H, Li Y, Qu L, Wu Y, Zeng C, Zhong W Tags: Biomed Res Int Source Type: research

Role of connexin 43 in cadmium ‐induced proliferation of human prostate epithelial cells
In this study, the human prostate epithelial cell line RWPE‐1 was exposed to Cd. A low dose of Cd stimulated cell proliferation along with a lower degree of gap junction intercellular communication and an elevated level of the protein Cx43. Cd exposure increased the levels of intracellular Ca2+ and phosphorylated Cx43 at the Ser368 site. Knockdown of Cx43 using siRNA blocked Cd‐induced proliferation and interfered with the Cd‐induced changes in the protein levels of cyclin D1, cyclin B1, p27Kip1 (p27) and p21Waf1/Cip1 (p21). The increase in Cx43 expression induced by Cd was presumably mediated by the androgen recepto...
Source: Journal of Applied Toxicology - February 7, 2017 Category: Toxicology Authors: Qingping Liu, Xiaoli Ji, Zehe Ge, Haipeng Diao, Xiuli Chang, Lihua Wang, Qing Wu Tags: Research article Source Type: research

SATB1 promotes prostate cancer metastasis by the regulation of epithelial-mesenchymal transition.
In this study SATB1 expression vector or siRNA was employed to modulate the expression level of SATB1 in prostate cancer cells and xenograft tumor in nude mouse model. Immunohistochemical analysis was performed on clinical prostate cancer samples. Silencing SATB1 inhibited the growth of DU-145 cells subcutaneous tumor in nude mice, while SATB1 overexpression promoted the growth of LNCaP cells subcutaneous tumor in nude mice. Immunohistochemical and Western blot analysis of the xenografts showed that silencing SATB1 led to decreased expression of vimentin and MMP2 and increased expression of E-cadherin, while SATB1 overexpr...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 31, 2016 Category: Drugs & Pharmacology Authors: Mao LJ, Yang CH, Fan L, Gao P, Yang DR, Xue BX, Zheng JN, Shan YX Tags: Biomed Pharmacother Source Type: research

27-Hydroxycholesterol stimulates cell proliferation and resistance to docetaxel-induced apoptosis in prostate epithelial cells
In this study, we determined the effect of 27-OHC in human prostate epithelial cells RWPE-1. We found that 27-OHC stimulates proliferation and increases androgen receptor (AR) transcriptional activity. 27-OHC also increased prostate-specific antigen expression and enhanced AR binding to the androgen response element compared to controls. Silencing AR expression with siRNA markedly reduced the 27-OHC-induced proliferation. Furthermore, 27-OHC blocked docetaxel-induced apoptosis. Altogether, our results suggest that 27-OHC may play an important role in PCa and BPH progression by promoting proliferation and suppressing apoptosis.
Source: Medical Oncology - January 5, 2016 Category: Cancer & Oncology Source Type: research

Potential role of the OPG/RANK/RANKL axis in prostate cancer invasion and bone metastasis.
Authors: Li X, Liu Y, Wu B, Dong Z, Wang Y, Lu J, Shi P, Bai W, Wang Z Abstract Receptor activator of NF-κB (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG) are key regulators of bone metabolism under both normal and pathological conditions, including prostate cancer (PCa) bone metastases. However, little is known concerning the expression and function of these regulators in prostate tumor samples and PCa cells and their correlation with invasion and bone metastasis. In the present study, we determined the expression of RANK, RANKL and OPG in 3 human PCa cell lines and 40 PCa patient samples by immunohistoche...
Source: Oncology Reports - November 16, 2014 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion.
Abstract SETDB1 has been established as an oncogene in a number of human carcinomas. The present study was to evaluate the expression of SETDB1 in prostate cancer (PCa) tissues and cells and to preliminarily investigate the role of SETDB1 in prostate tumorigenesis in vitro. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the expression of SETDB1 in PCa tissues, adjacent normal tissues, benign prostatic hyperplasia (BPH) tissues, PCa cell lines and normal prostate epithelial cells. The results suggested that SETDB1 was upregulated in human PC...
Source: Asian Journal of Andrology - February 18, 2014 Category: Urology & Nephrology Authors: Sun Y, Wei M, Ren SC, Chen R, Xu WD, Wang FB, Lu J, Shen J, Yu YW, Hou JG, Xu CL, Huang JT, Sun YH Tags: Asian J Androl Source Type: research

The over‐expression of Pim‐2 promote the tumorigenesis of prostatic carcinoma through phosphorylating eIF4B
CONCLUSIONPim‐2 was over‐expressed in PCA cell lines and tissues. It may inhibit the apoptosis of PCA cells through phosphorylating eIF4B, thus promote the tumorigenesis of PCA. Prostate © 2013 Wiley Periodicals, Inc.
Source: The Prostate - June 27, 2013 Category: Urology & Nephrology Authors: Ke Ren, Xin Gou, Mingzhao Xiao, Ming Wang, Chaodong Liu, Zhaobing Tang, Weiyang He Tags: Original Article Source Type: research

The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor
Conclusions: Our study suggests reciprocal regulation of PlncRNA-1 and androgen receptor contribute to CaP pathogenesis and that PlncRNA-1 is a potential therapy target.
Source: Urologic Oncology: Seminars and Original Investigations - January 24, 2012 Category: Urology & Nephrology Authors: Zilian Cui, Shancheng Ren, Ji Lu, Fubo Wang, Weidong Xu, Yi Sun, Min Wei, Junyi Chen, Xu Gao, Chuanliang Xu, Jian-Hua Mao, Yinghao Sun Tags: Laboratory - Prostate Source Type: research