Analysis of the expression and association of retinoblastoma binding protein 6 (RBBP6) with the JNK signaling pathway in prostate cancers.

This study aims to investigate the expression of retinoblastoma binding protein 6 (RBBP6) in prostate cancer (PCa) and its association with the c-Jun N-terminal kinase (JNK) pathway. Immunohistochemistry was used to detect RBBP6 and JNK1/2 expression in PCa and benign prostatic hyperplasia (BPH) tissues. RBBP6 expression in PCa cells (LNCap, PC3 and DU145) and noncancerous prostate epithelial cells (RWPE-1) was determined by qRT-PCR and Western blotting. PC3 and DU145 cells were transfected with RBBP6 siRNAs to examine the biological characteristics. Anisomycin (a JNK activator) with/without RBBP6 siRNA was used to treat PC3 cells for further investigating the ramification of the RBBP6-mediated JNK pathway in PCa. PCa tissues and cells showed higher RBBP6 and JNK1/2 expression. RBBP6 was positively correlated with JNK1/2 in PCa tissues. Besides, RBBP6 expression was correlated to clinical tumor stage, lymph node metastasis, Gleason grade, preoperative PSA level, as well as prognosis of PCa. RBBP6 siRNA reduced cell proliferation, arrested cells at G2/M, and promoted cell apoptosis, and suppressed JNK pathway. Additionally, migration and invasion decreased after the RBBP6 siRNA transfection with downregulated MMP-2 and MMP-9. Anisomycin promoted the proliferation, invasion and migration of PC3 cells and inhibited PC3 cell apoptosis, which could be reversed by RBBP6 siRNA. RBBP6 expression was upregulated in PCa tissues and positively correlated with expression level of JNK1/2....
Source: Cell Biology International - Category: Cytology Authors: Tags: Cell Biol Int Source Type: research