Filtered By:
Specialty: Cytology
Condition: Osteoporosis

This page shows you your search results in order of date.

Order by Relevance | Date

Total 18 results found since Jan 2013.

The histone acetyltransferase Mof regulates Runx2 and Osterix for osteoblast differentiation
AbstractOsteoblast differentiation is regulated by various transcription factors, signaling molecules, and posttranslational modifiers. The histone acetyltransferase Mof (Kat8) is involved in distinct physiological processes. However, the exact role of Mof in osteoblast differentiation and growth remains unknown. Herein, we demonstrated that Mof expression with histone H4K16 acetylation increased during osteoblast differentiation. Inhibition of Mof by siRNA knockdown or small molecule inhibitor, MG149 which is a potent histone acetyltransferase inhibitor, reduced the expression level and transactivation potential of osteog...
Source: Cell and Tissue Research - May 29, 2023 Category: Cytology Source Type: research

Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis via Wnt/ β-catenin signaling pathway
CONCLUSIONS: Dnmt3a silencing rescues the negative effects of DOP on ASCs and provides a possible approach for bone tissue regeneration in patients with diabetic osteoporosis.PMID:35927735 | DOI:10.1186/s13287-022-03088-4
Source: Cell Research - August 4, 2022 Category: Cytology Authors: Maorui Zhang Yujin Gao Qing Li Huayue Cao Jianghua Yang Xiaoxiao Cai Jingang Xiao Source Type: research

RNA interference-based osteoanabolic therapy for osteoporosis by a bone-formation surface targeting delivery system
In this study, we identified casein kinase-2 interacting protein-1 encoding gene (Ckip-1), a negative regulator of bone formation, as an effective target of small interfering RNAs (siRNAs) for improving bone mass. Moreover, an impressive (DSS)6-Liposome (Lipos) nanoparticle system that could target the bone formation surface was synthesized to enhance the delivery of Ckip-1 siRNA to osteogenic lineage cells. The in vitro results confirmed that the (DSS)6-Lipos system could efficaciously improve the intracellular delivery of Ckip-1 siRNA without obvious cell toxicity. The in vivo application of the delivery system showed sp...
Source: Cell Research - March 1, 2022 Category: Cytology Authors: Ye Gao He Xin Bolei Cai Le Wang Qianxin Lv Yan Hou Fuwei Liu Taiqiang Dai Liang Kong Source Type: research

ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF κB activation
Cell Mol Life Sci. 2022 Feb 26;79(3):155. doi: 10.1007/s00018-022-04186-5.ABSTRACTCellular senescence is closely related to tissue aging including bone. Bone homeostasis is maintained by the tight balance between bone-forming osteoblasts and bone-resorbing osteoclasts, but it undergoes deregulation with age, causing age-associated osteoporosis, a main cause of which is osteoblast dysfunction. Oxidative stress caused by the accumulation of reactive oxygen species (ROS) in bone tissues with aging can accelerate osteoblast senescence and dysfunction. However, the regulatory mechanism that controls the ROS-induced senescence o...
Source: Cellular and Molecular Life Sciences : CMLS - February 26, 2022 Category: Cytology Authors: Hyun-Jung Kim Woo-Jin Kim Hye-Rim Shin Hee-In Yoon Jae-I Moon Eunji Lee Jin-Muk Lim Young-Dan Cho Mi-Hye Lee Hong-Gee Kim Hyun-Mo Ryoo Source Type: research

A dual role of HIF1 α in regulating osteogenesis-angiogenesis coupling
CONCLUSION: The dual role of HIF1α in osteogenesis-angiogenesis coupling may depend on the ROS-mediated HIF1α-p53 relationship. New awareness about HIF1α will be conducive to its future application in senile osteoporosis.PMID:35123567 | DOI:10.1186/s13287-022-02742-1
Source: Cell Research - February 6, 2022 Category: Cytology Authors: Jingjing Shao Shibo Liu Min Zhang Shujiang Chen Shuaiqi Gan Chenfeng Chen Wenchuan Chen Lei Li Zhimin Zhu Source Type: research

Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/ β-catenin signaling
This study has unraveled that MTSS1 contributes to osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling. It also suggests the potential of MTSS1 as a new target for the treatment of osteoporosis.PMID:35094173 | DOI:10.1007/s00018-022-04147-y
Source: Cellular and Molecular Life Sciences : CMLS - January 30, 2022 Category: Cytology Authors: Meng Chen Liying Shan Ying Gan Lijie Tian Jie Zhou Endong Zhu Hairui Yuan Xiaoxia Li Baoli Wang Source Type: research

HDAC6 inactivates Runx2 promoter to block osteogenesis of bone marrow stromal cells in age-related bone loss of mice
CONCLUSION: HDAC6 accumulation and histone hypoacetylation on Runx2 promoter contributed to the attenuation of in vitro osteogenic differentiation potential of BMSCs from aged mice. Through HDAC6 inhibition, we could activate Runx2 expression and osteogenic differentiation potential of BMSCs from aged mice and alleviate the age-related bone loss of aged mice. Our study will benefit not only for understanding the age-related bone loss, but also for finding new therapies to treat senile osteoporosis.PMID:34454588 | DOI:10.1186/s13287-021-02545-w
Source: Cell Research - August 29, 2021 Category: Cytology Authors: Chao Ma Juan Gao Jun Liang Weixiang Dai Zhenfei Wang Mengjiao Xia Tao Chen Sen Huang Jian Na Long Xu Shiming Feng Kerong Dai Guangwang Liu Source Type: research

Tissue inhibitor of metalloproteinase 1 suppresses growth and differentiation of osteoblasts and differentiation of osteoclasts by targeting the AKT pathway.
In conclusion, our results demonstrate that TIMP1 can act as a suppressor of growth and differentiation of osteoblasts and differentiation of osteoclasts through the negative regulation of the AKT pathway. We propose that TIMP1 may serve as a potential target for low bone mass-related skeletal diseases, such as osteoporosis. PMID: 32113948 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - February 26, 2020 Category: Cytology Authors: Xi Y, Huang H, Zhao Z, Ma J, Chen Y Tags: Exp Cell Res Source Type: research

Pulsed electromagnetic fields regulate osteocyte apoptosis, RANKL/OPG expression, and its control of osteoclastogenesis depending on the presence of primary cilia
This study enriches our basic knowledge for further understanding the biological behaviors of osteocytes, and is also helpful for providing a more comprehensive mechanistic understanding of the effect of electromagnetic stimulation on bone and relevant skeletal diseases (e.g., bone fractu re and osteoporosis). AbstractGrowing evidence has shown that pulsed electromagnetic fields (PEMF) can modulate bone metabolism in vivo and regulate the activities of osteoblasts and osteoclasts in vitro. Osteocytes, accounting for 95% of bone cells, act as the major mechanosensors in bone for transducing external mechanical signals and p...
Source: Journal of Cellular Physiology - November 13, 2018 Category: Cytology Authors: Pan Wang, Chi Tang, Junjie Wu, Yuefan Yang, Zedong Yan, Xiyu Liu, Xi Shao, Mingming Zhai, Jie Gao, Shengru Liang, Erping Luo, Da Jing Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Combined strategy of siRNA and osteoclast actin cytoskeleton automated imaging to identify novel regulators of bone resorption shows a non-mitotic function for anillin.
Abstract Osteoclasts are the main cells responsible for the resorption of mineralized extracellular matrices. They are the major targets for anti-resorptive therapies to manage osteoporosis, a major public health problem. Osteoclasts are giant multinucleated cells that can organize their a unique adhesion structure based on a belt of podosomes, which is the keystone of the bone resorption apparatus. We combined differential transcriptomics and siRNA screening approaches to get a broader view of cytoskeletal regulators that participate in the control of osteoclast cytoskeleton and identify novel regulators of bone ...
Source: European Journal of Cell Biology - October 31, 2018 Category: Cytology Authors: Maurin J, Morel A, Hassen-Khodja C, Vives V, Jurdic P, Machuca-Gayet I, Blangy A Tags: Eur J Cell Biol Source Type: research

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Abstract Low-density lipoprotein receptor-related protein 4 (LRP4) is a multi-functional protein implicated in bone, kidney and neurological diseases including Cenani-Lenz syndactyly (CLS), sclerosteosis, osteoporosis, congenital myasthenic syndrome and myasthenia gravis. Why different LRP4 mutation alleles cause distinct and even contrasting disease phenotypes remain unclear. Herein, we utilized the zebrafish model to search for pathways affected by a deficiency of LRP4. The lrp4 knockdown in zebrafish embryos exhibits cyst formations at fin structures and the caudal vein plexus, malformed pectoral fins, defectiv...
Source: Cellular and Molecular Life Sciences : CMLS - October 16, 2018 Category: Cytology Authors: Tian J, Shao J, Liu C, Hou HY, Chou CW, Shboul M, Li GQ, El-Khateeb M, Samarah OQ, Kou Y, Chen YH, Chen MJ, Lyu Z, Chen WL, Chen YF, Sun YH, Liu YW Tags: Cell Mol Life Sci Source Type: research

LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3
Conclusions: Taken together, the key findings of the present study suggest that LINC00311 induces proliferation and inhibits apoptosis of osteoclasts via the regulation of the Notch signaling pathway by inhibiting DLL3 expression, ultimately demonstrating that LINC00311 and its target gene DLL3 may serve as independent factors in cases of osteoporosis.Cell Physiol Biochem 2018;47:2291 –2306
Source: Cellular Physiology and Biochemistry - July 5, 2018 Category: Cytology Source Type: research

Liraglutide, a glucagon-like peptide-1 receptor agonist, facilitates osteogenic proliferation and differentiation in MC3T3-E1 cells through phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), extracellular signal-related kinase (ERK)1/2, and cAMP/protein kinase A (PKA) signaling pathways involving β-catenin.
Abstract Previous studies have proven that Glucagon-like peptide-1 (GLP-1) and its receptor agonist exert favorable anabolic effects on skeletal metabolism. However, whether GLP-1could directly impact osteoblast-mediated bone forming is still controversial, and the underlying molecular mechanism remains to be elucidated. Thus in this paper, we investigated the effects of liraglutide, a glucagon-like peptide-1 (GLP-1) receptor agonist, on murine MC3T3-E1 preosteoblasts proliferation and differentiation and explored the potential cellular basis. Our study confirmed the presence of GLP-1R in MC3T3-E1, and demonstrate...
Source: Experimental Cell Research - September 14, 2017 Category: Cytology Authors: Wu X, Li S, Xue P, Li Y Tags: Exp Cell Res Source Type: research