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Condition: Glaucoma

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Total 87 results found since Jan 2013.

Expression of retinoic acid signaling components ADH7 and ALDH1A1 is reduced in aniridia limbal epithelial cells and a siRNA primary cell based aniridia model.
Abstract PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. First, we sequenced mRNA from limbal epithelial cells isolated from controls and aniridia patients. Second, we confirmed the bioinformatics and literature-based result list for a small interfering RNA (siRNA)-based primary aniridia cell model (PAX6 knockdown). With this approach, we expected tha...
Source: Experimental Eye Research - October 4, 2018 Category: Opthalmology Authors: Latta L, Nordström K, Stachon T, Langenbucher A, Fries FN, Szentmáry N, Seitz B, Käsmann-Kellner B Tags: Exp Eye Res Source Type: research

Intracameral Delivery of Layer ‐by‐Layer Coated siRNA Nanoparticles for Glaucoma Therapy
Nanoparticles loaded with siRNA against connective tissue growth factor (CTGF) and coated with a final layer of hyaluronan (HA) are proposed as a novel and causative approach for glaucoma treatment. HA ‐coated nanoparticles deeply penetrate into the outflow region of porcine, murine, and human eyes ex vivo and have the capability of silencing CTGF in cultures of human trabecular meshwork cells. AbstractGlaucoma is the second leading cause of blindness worldwide, often associated with elevated intraocular pressure. Connective tissue growth factor (CTGF) is a mediator of pathological effects in the trabecular meshwork (TM)...
Source: Small - October 23, 2018 Category: Nanotechnology Authors: Andrea E. Dillinger, Michaela Guter, Franziska Froemel, Gregor R. Weber, Kristin Perkumas, W. Daniel Stamer, Andreas Ohlmann, Rudolf Fuchshofer, Miriam Breunig Tags: Full Paper Source Type: research

Beta2 adrenergic receptor silencing change intraocular pressure in New Zealand rabbits
Conclusion The use of siRNA against the beta2 adrenergic receptors could provide an interesting therapeutic strategy for glaucoma treatment.
Source: Journal of Optometry - November 11, 2017 Category: Opthalmology Source Type: research

GSE113008 mRNA sequencing of Aniridia limbal epithelial cells.
Contributors : Karl Nordstr öm ; Jasmin KirchSeries Type : Expression profiling by high throughput sequencingOrganism :PAX6-related Aniridia is a sight-threatening disease due to progression of secondary glaucoma and aniridia associated keratopathy (AAK). Changes or loss of limbal epithelial progenitors causes the epithelial surfaces defects. We analyzed how PAX6 contribute to this with a two-step approach. 1) mRNA Sequencing of limbal epithelial cells isolated from controls and aniridia patients. 2) confirming the bioinformatical and literature-based result list on a siRNA based primary aniridia cell model (PAX6- knockdo...
Source: GEO: Gene Expression Omnibus - October 24, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Source Type: research

Evaluation of Adenovirus-Mediated Down-Regulation of Connective Tissue Growth Factor on Postoperative Wound Healing After Experimental Glaucoma Surgery.
CONCLUSIONS: Down-regulation of CTGF is effective in inhibiting postoperative scarring in vivo. This suggests that RNAi with CTGF siRNA may potentially pave the road for a novel therapeutic strategy to improve glaucoma surgery results. PMID: 26554857 [PubMed - as supplied by publisher]
Source: Current Eye Research - November 10, 2015 Category: Opthalmology Authors: Lim DH, Kim TE, Kee C Tags: Curr Eye Res Source Type: research

Inhibition of heat shock protein 47 suppressed collagen production in Tenon's capsule fibroblasts
CONCLUSIONS: HSP47 siRNA inhibits the production of COLI in rat Tenon's fibroblasts, and may be the potential therapeutic method in bleb scarring after glaucoma filtration surgery.PMID:37724035 | DOI:10.5603/fhc.96514
Source: Folia Histochemica et Cytobiologica - September 19, 2023 Category: Cytology Authors: Weiwei Wang Haiyan lI Source Type: research

Dexamethasone Increases Cdc42 Expression in Human TM-1 Cells.
Conclusions: DEX increases Cdc42 expression in TM-1. This may represent a potential mechanism of DEX-induced HTM cytoskeletal rearrangement. PMID: 24871483 [PubMed - as supplied by publisher]
Source: Current Eye Research - May 28, 2014 Category: Opthalmology Authors: Qiu X, Wu K, Lin X, Liu Q, Ye Y, Yu M Tags: Curr Eye Res Source Type: research

TRPV4 is activated by mechanical stimulation to induce prostaglandins release in trabecular meshwork, lowering intraocular pressure
by Takatoshi Uchida, Shota Shimizu, Reiko Yamagishi, Suzumi M. Tokuoka, Yoshihiro Kita, Rei Sakata, Megumi Honjo, Makoto Aihara Trabecular meshwork constitutes the conventional outflow pathway and controls intraocular pressure by regulating aqueous outflow. Mechanical stimulation has been studied as one of the triggers to regulate aqueous outflow in trabecular meshwork, but it is not well understood. We investigated that h ow transient receptor potential cation channel subfamily V member 4 (TRPV4) functions in human trabecular meshwork cells (HTMC) and affects intraocular pressure (IOP). HTMC were treated with TRPV4 siRNA...
Source: PLoS One - October 21, 2021 Category: Biomedical Science Authors: Takatoshi Uchida Source Type: research

Gene transfer to the outflow tract.
Abstract Elevated intraocular pressure is the primary cause of open angle glaucoma. Outflow resistance exists within the trabecular meshwork but also at the level of Schlemm's canal and further downstream within the outflow system. Viral vectors allow to take advantage of naturally evolved, highly efficient mechanisms of gene transfer, a process that is termed transduction. They can be produced at biosafety level 2 in the lab using protocols that have evolved considerably over the last 15-20 years. Applied by an intracameral bolus, vectors follow conventional as well as uveoscleral outflow pathways. They may affec...
Source: Experimental Eye Research - April 26, 2016 Category: Opthalmology Authors: Dang Y, Loewen R, Parikh HA, Roy P, Loewen NA Tags: Exp Eye Res Source Type: research

TGF- β-induced IOP elevations are mediated by RhoA in the early but not the late fibrotic phase of open angle glaucoma.
Conclusions: We conclude that RhoA signaling mediates the early IOP rise induced by TM cellular changes associated with contractility but not the sustained IOP elevation caused by TM fibrosis. Thus, RhoA therapies offer a clinically relevant opportunity for IOP management, likely through the modulation of TM contractility, but appear to be ineffective in the amelioration of fibrosis. PMID: 30429640 [PubMed - in process]
Source: Molecular Vision - November 16, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research