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Condition: Age-Related Macular Degeneration (AMD)

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Total 41 results found since Jan 2013.

Delivery systems for siRNA drug development in cancer therapy
Publication date: Available online 28 August 2014 Source:Asian Journal of Pharmaceutical Sciences Author(s): Cong-fei Xu , Jun Wang Since the discovery of the Nobel prize-winning mechanism of RNA interference (RNAi) ten years ago, it has become a promising drug target for the treatment of multiple diseases, including cancer. There have already been some successful applications of siRNA drugs in the treatment of age-related macular degeneration and respiratory syncytial virus infection. However, significant barriers still exist on the road to clinical applications of siRNA drugs, including poor cellular uptake, instabilit...
Source: Asian Journal of Pharmaceutical Sciences - October 12, 2014 Category: Drugs & Pharmacology Source Type: research

Adeno-associated virus type 8 vector-mediated expression of siRNA targeting vascular endothelial growth factor efficiently inhibits neovascularization in a murine choroidal neovascularization model.
CONCLUSIONS: These results suggest that siRNA-VEGF can be expressed across the retina and that long-term suppression of CNV is possible through the use of stable AAV2/8-mediated siRNA-VEGF expression. In vivo gene therapy may thus be a feasible approach to the clinical management of CNV in conditions such as age-related macular degeneration. PMID: 24744609 [PubMed - indexed for MEDLINE]
Source: Molecular Vision - November 16, 2014 Category: Molecular Biology Tags: Mol Vis Source Type: research

Intravitreal Ets1 siRNA alleviates choroidal neovascularization in a mouse model of age-related macular degeneration
In this study, Ets1 increased and localized in the macrophages and microglia of a mouse laser-induced CNV region. Ets1 siRNA intravitreal injection ameliorated the leakage and area of CNV, as well as inhibiting the dysfunction of retinal pigment epithelium (RPE) cells and the activation of macrophages/microglia. Taken together, we provide a new insight into the molecular mechanism of CNV progression, in which Ets1 can be a new therapeutic target.
Source: Cell and Tissue Research - February 28, 2019 Category: Cytology Source Type: research

Oligonucleotides —A Novel Promising Therapeutic Option for IBD
Conclusions In this review, we focused on recent and past approaches to test the therapeutic efficacy of oligonucleotide based therapies in IBD. The combining mechanistic mode of oligonucleotide based therapeutics is a targeted action on specific pro-inflammatory molecules, which are over activated in IBD patients and contribute significantly to disease pathogenesis. The proposed high selectivity of the agents is derived from its mode of action, that aims to specifically block certain inflammatory molecular patterns, without a general systemic effect on other molecular targets. It would be important for each oligonucleot...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research

Concerted inhibition of HIF-1α and -2α expression markedly suppresses angiogenesis in cultured RPE cells.
Abstract HIF-1α is known to play an important role in the induction of VEGF by hypoxia in retinal pigment epithelial (RPE) cells. However, the involvement of the other isoform, HIF-2α, in RPE cells remains unclear. Thus, the purpose of present study was to clarify the role of HIF-2α during induction of angiogenic genes in hypoxic RPE cells. When human RPE cells (ARPE-19) were cultured under hypoxic conditions, HIF-1α and HIF-2α proteins increased. This induced an increase in mRNA for VEGF, causing secretion of VEGF protein into the medium. This conditioned medium induced tube formation in human vascular endot...
Source: Molecular and Cellular Biochemistry - July 20, 2013 Category: Biochemistry Authors: Nakajima T, Nakajima E, Shearer TR, Azuma M Tags: Mol Cell Biochem Source Type: research

Inhibition of YAP ameliorates choroidal neovascularization via inhibiting endothelial cell proliferation.
Conclusions: This study showed that YAP upregulation promoted CNV formation by upregulating the proliferation of endothelial cells, providing evidence for the molecular mechanisms of CNV and suggesting a novel molecular target for nAMD treatment. PMID: 29422766 [PubMed - in process]
Source: Molecular Vision - February 11, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research

Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression.
Conclusions: The expression of the C9 gene in cultured RPE cells is highly induced by extracellular hyperosmolarity, hypoxia, and oxidative stress. The data may support the assumption that C9 gene expression may stimulate the expression of inflammatory (NLRP3) and angiogenic growth factors (VEGF) in RPE cells. Extracellular C9 protein may attenuate this effect, in part via negative regulation of the C9 mRNA level. PMID: 30090015 [PubMed - in process]
Source: Molecular Vision - August 11, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research

Intracellular uptake mechanism of lutein in retinal pigment epithelial cells.
CONCLUSION: The uptake of lutein was significantly decreased by 40% compared with the control uptake level. This suggested that active transport of lutein into ARPE-19 cells is mainly via SR-B1, given the result that lutein uptake at 4ºC was about 40% less that that at 37ºC. PMID: 24021296 [PubMed - indexed for MEDLINE]
Source: Journal of Pharmacy and Pharmaceutical Sciences - December 2, 2014 Category: Drugs & Pharmacology Tags: J Pharm Pharm Sci Source Type: research

Involvement of Intracellular Calcium Mobilization in IL-8 Activation in Human Retinal Pigment Epithelial Cells Biochemistry and Molecular Biology
Conclusions. Collectively, our findings highlight the important role of intracellular calcium mobilization in the activation of IL-8. These findings may be helpful for the clinical applications in the age-related macular degeneration (AMD) prevention and treatment.
Source: Investigative Ophthalmology - February 3, 2015 Category: Opthalmology Authors: Yang, I.-H., Wong, J.-H., Chang, C.-M., Chen, B.-K., Tsai, Y.-T., Chen, W.-C., Wang, E. T., Hsu, W.-L., Chang, W.-C. Tags: Biochemistry and Molecular Biology Source Type: research

TNF-α mediates choroidal neovascularization by upregulating VEGF expression in RPE through ROS-dependent β-catenin activation.
CONCLUSIONS: Our results support the thinking that TNF-α contributes to CNV by upregulating VEGF production in RPE cells through ROS-dependent activation of β-catenin signaling. These results provide mechanisms of crosstalk between inflammatory mediator, TNF-α, and ROS in RPE cells. PMID: 26900328 [PubMed - in process]
Source: Molecular Vision - February 25, 2016 Category: Molecular Biology Tags: Mol Vis Source Type: research

Lycopene inhibits ICAM-1 expression and NF-κB activation by Nrf2-regulated cell redox state in human retinal pigment epithelial cells
Publication date: Available online 4 May 2016 Source:Life Sciences Author(s): Po-Min Yang, Zhi-Zhen Wu, Yu-Qi Zhang, Being-Sun Wung Aims Age-related macular degeneration (AMD) is one of the most common diseases leading to blindness in elderly people. The progression of AMD may be prevented through anti-inflammation and antioxidation in retinal pigment epithelial (RPE) cells. Lycopene, a carotenoid, has been shown to possess both antioxidative and anti-inflammatory properties. This research was conducted to detail the mechanisms of these effects of lycopene-treated RPE cells. Main methods We exposed ARPE-19 cells to ...
Source: Life Sciences - May 4, 2016 Category: Biology Source Type: research

Up-regulation of P-gp via NF- κB activation confers protection against oxidative damage in the retinal pigment epithelium cells.
In conclusion, P-gp can provide moderate cytoprotection for the human RPE cells by ameliorating the mitochondrial dysfunction and NF-κB activation may be a potential regulator of P-gp expression response to oxidative stress. PMID: 30496729 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - November 26, 2018 Category: Opthalmology Authors: Feng Q, Yang W, Gao Z, Ruan X, Zhang Y Tags: Exp Eye Res Source Type: research

RNA therapeutics in ophthalmology - translation to clinical trials.
Abstract The use of RNA interference technology has proven to inhibit the expression of many target genes involved in the underlying pathogenesis of several diseases affecting various systems. First established in in vitro and later in animal studies, small interfering RNA (siRNA) and antisense oligonucleotide (ASO) therapeutics are now entering clinical trials with the potential of clinical translation to patients. Gene-silencing therapies have demonstrated promising responses in ocular disorders, predominantly due to the structure of the eye being a closed and compartmentalised organ. However, although the effic...
Source: Experimental Eye Research - February 3, 2021 Category: Opthalmology Authors: Gupta A, Kafetzis KN, Tagalakis AD, Yu-Wai-Man C Tags: Exp Eye Res Source Type: research

Inhibition of AIF-1 alleviates laser-induced macular neovascularization by inhibiting endothelial cell proliferation via restrained p44/42 MAPK signaling pathway
This study aimed to understand how AIF-1 effects the proliferation of CECs and the subsequent progression of MNV. To study this, a mouse MNV model was established through laser injury, and the AIF-1 expression levels were then measured using western blot and immunohistochemistry. AIF-1 siRNA was intravitreally injected to silence AIF-1 gene expression. Western blot and choroidal flat mount were performed to measure the progression of MNV and proliferation of the CECs. These results showed that the protein expression of AIF-1 was significantly elevated in the laser-induced mouse MNV model, and the expression trend was consi...
Source: Experimental Eye Research - April 18, 2023 Category: Opthalmology Authors: Qinghua Li Liting Hu Guibo Liu Xiaoni Yin Ying Li Xiangyang Wei Ning Duan Xiaoran Zhao Qingyun Gong Zhaodong Du Source Type: research