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Total 135484 results found since Jan 2013.
Microbiome-targeted interventions for the control of oral-gut dysbiosis and chronic systemic inflammation
Trends Mol Med. 2023 Sep 18:S1471-4914(23)00191-0. doi: 10.1016/j.molmed.2023.08.006. Online ahead of print.ABSTRACTRecent research has confirmed the strong connection between imbalances in the oral and gut microbiome (oral-gut dysbiosis), periodontitis, and inflammatory conditions such as diabetes, Alzheimer's disease, and cardiovascular diseases. Microbiome modulation is crucial for preventing and treating several autoimmune and inflammatory diseases, including periodontitis. However, the causal relationships between the microbiome and its derived metabolites that mediate periodontitis and chronic inflammation constitute...
Source: Trends in Molecular Medicine - September 20, 2023 Category: Molecular Biology Authors: Juan Pacheco-Yanes Eric Reynolds Jian Li Eliana Mari ño Source Type: research
Protein kinase signaling networks driven by oncogenic Gq/11 in uveal melanoma identified by phosphoproteomic and bioinformatic analyses
Mol Cell Proteomics. 2023 Sep 18:100649. doi: 10.1016/j.mcpro.2023.100649. Online ahead of print.ABSTRACTMetastatic uveal melanoma (mUM) patients typically survive only 2-3 years because effective therapy does not yet exist. Here, to facilitate the discovery of therapeutic targets in UM, we have identified protein kinase signaling mechanisms elicited by the drivers in 90% of UM tumors: mutant constitutively active G protein α-subunits encoded by GNAQ (Gq) or GNA11 (G11). We used the highly specific Gq/11 inhibitor FR900359 (FR) to elucidate signaling networks that drive proliferation, metabolic reprogramming, and dediffer...
Source: Molecular and Cellular Proteomics : MCP - September 20, 2023 Category: Molecular Biology Authors: Michael D Onken Petra Erdmann-Gilmore Qiang Zhang Kisan Thapa Emily King Kevin M Kaltenbronn Sarah E Noda Carol M Makepeace Dennis Goldfarb Özgün Babur R Reid Townsend Kendall J Blumer Source Type: research
Clinical Proteomics for Solid Organ Tissues
Mol Cell Proteomics. 2023 Sep 18:100648. doi: 10.1016/j.mcpro.2023.100648. Online ahead of print.ABSTRACTThe evaluation of biopsied solid organ tissue has long relied on visual examination using a microscope. Immunohistochemistry is critical in this process, labeling and detecting cell lineage markers and therapeutic targets. However, while the practice of immunohistochemistry has reshaped diagnostic pathology and facilitated improvements in cancer treatment, it has also been subject to pervasive challenges with respect to standardization and reproducibility. Efforts are ongoing to improve immunohistochemistry, but for som...
Source: Molecular and Cellular Proteomics : MCP - September 20, 2023 Category: Molecular Biology Authors: William S Phipps Mark R Kilgore Jacob J Kennedy Jeffrey R Whiteaker Andrew N Hoofnagle Amanda G Paulovich Source Type: research
Comparison of two protocols for the generation of iPSC-derived human astrocytes
CONCLUSIONS: Overall, our analyses highlight the need to consider the advantages and disadvantages of a given differentiation protocol, when designing functional or drug discovery studies involving iPSC-derived astrocytes.PMID:37730545 | PMC:PMC10512486 | DOI:10.1186/s12575-023-00218-x
Source: Biological Procedures Online - September 20, 2023 Category: Molecular Biology Authors: Patrycja Mulica Carmen Venegas Zied Landoulsi Katja Badanjak Sylvie Delcambre Maria Tziortziou Soraya Hezzaz Jenny Ghelfi Semra Smajic Jens Schwamborn Rejko Kr üger Paul Antony Patrick May Enrico Glaab Anne Gr ünewald Sandro L Pereira Source Type: research
Upregulation of FAM134B inhibits endoplasmic reticulum stress-related degradation protein expression and promotes hepatocellular carcinogenesis
J Cell Mol Med. 2023 Sep 20. doi: 10.1111/jcmm.17964. Online ahead of print.ABSTRACTEndoplasmic reticulum (ER) stress can stimulate the proliferation and metastasis of hepatocellular carcinoma (HCC) cells while hindering apoptosis and immune system function, but the molecular mechanism of ER stress in HCC has yet to be fully studied. We aim to investigate the molecular mechanism by which FAM134B inhibits autophagy of HCC cells by reducing the expression of ER stress-related degradation proteins. Clinical samples were collected for this study. Normal liver cell lines HL7702 and Hep3B and Huh7 HCC cell lines were cultured. C...
Source: J Cell Mol Med - September 20, 2023 Category: Molecular Biology Authors: Houhong Wang Lu Liu Huihui Gong Heng Li Source Type: research
Selenium in combination with a tomato lipid extract as a therapy for benign prostatic hyperplasia and its alterations in rats with induced BPH
J Cell Mol Med. 2023 Sep 19. doi: 10.1111/jcmm.17903. Online ahead of print.ABSTRACTBenign prostatic hyperplasia (BPH) is the most common adenoma in old men. Tomatoes are a rich source of bioactive compounds that, as well as selenium (Se), possess antioxidant and antiproliferative activity. The aim was to evaluate the therapeutic effect of Se in combination with a tomato extract in aged rats with BPH. Aged male Wistar rats were divided in the following groups (n = 10 rats/group): Control (C), BPH, BPH + Finasteride (BPH + F), BPH + Tomato Lipidic Extract (BPH + E), BPH + Selenium (BPH + S) and BPH plus E plus S (BPH + E + ...
Source: J Cell Mol Med - September 20, 2023 Category: Molecular Biology Authors: David Julian Arias-Ch ávez Patrick Mailloux-Salinas Jessica Ledesma Aparicio Elihu Campos-P érez Omar Noel Medina-Campos Jos é Pedraza-Chaverri Guadalupe Bravo Source Type: research
Mineral elements and adiposity-related consequences in adolescents with intellectual disabilities
CONCLUSION: The intellectual disability of moderate type (MID) was associated with abnormal changes in the levels of essential mineral elements and adipokines and increased levels of cellular oxidative stress. Thus, evaluating plasma mineral elements and adipokines levels could be a potential diagnostic parameter for diagnosing MID.PMID:37730529 | PMC:PMC10512604 | DOI:10.1186/s12860-023-00490-5
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Ahmad H Alghadir Sami A Gabr Amir Iqbal Source Type: research
Characterization of a (p)ppApp synthetase belonging to a new family of polymorphic toxin associated with temperate phages
In this study, we characterized the MuF-RelA_SpoT toxin associated with the temperate phage of Streptococcus pneumoniae SPNA45. We show that the RelA_SpoT domain has (p)ppApp synthetase activity, which is bactericidal under our experimental conditions. We further determine that the two genes located downstream encode two immunity proteins, one binding to and inactivating the toxin and the other detoxifying the cell via a pppApp hydrolase activity. Finally, based on protein sequence alignments, we propose a signature for (p)ppApp synthetases that distinguishes them from (p)ppGpp synthetases.PMID:37730083 | DOI:10.1016/j.jmb.2023.168282
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Julia Bartoli Audrey C Tempier Noa L Guzzi Chlo é M Piras Eric Cascales Julie Pm Viala Source Type: research
The smallest infectious substructure encoding the prion strain structural determinant revealed by spontaneous dissociation of misfolded prion protein assemblies
J Mol Biol. 2023 Sep 18:168280. doi: 10.1016/j.jmb.2023.168280. Online ahead of print.ABSTRACTIt is commonly accepted that the prion replicative propensity and strain structural determinant (SSD) are encoded in the fold of PrPSc amyloid fibril assemblies. By exploring the quaternary structure dynamicity of several prion strains, we revealed that all mammalian prion assemblies exhibit the generic property of spontaneously generating two sets of discreet infectious tetrameric and dimeric species differing significantly by their specific infectivity. By using perturbation approaches such as dilution and ionic strength variati...
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Jan Bohl Mohammed Moudjou Laetitia Herzog Fabienne Reine Fiona Sailler Hannah Klute Frederic Halgand Guillaume Van der Rest Yves Boulard Vincent B éringue Angelique Igel Human Rezaei Source Type: research
Maresin-1 prevents blood-spinal cord barrier disruption associated with TRPV4 elevation in the experimental model of spinal cord injury
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Sep 18:159395. doi: 10.1016/j.bbalip.2023.159395. Online ahead of print.ABSTRACTRecently, we reported the TRPV4 ion channel activation and its association with secondary damage after spinal cord injury (SCI). TRPV4 activation is linked with blood-spinal cord barrier (BSCB) disruption, endothelial damage, and inflammation after SCI. Specialized pro-resolving mediators (SPM) are endogenous lipid mediators released for inflammation resolution. Studies suggest that SPM could act as an endogenous antagonist of ion channels directly or indirectly at the plasma membrane. Herein, we ...
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Zarna Pathak Tarang Jadav Abhishek Roy Manjeet Chopra Nidhi Singh Pinaki Sengupta Hemant Kumar Source Type: research
Exploring The "Misfolding Problem" by Systematic Discovery and Analysis of Functional-But-Degraded Proteins
Mol Biol Cell. 2023 Sep 20:mbcE23060248. doi: 10.1091/mbc.E23-06-0248. Online ahead of print.ABSTRACTIn both health and disease, the ubiquitin-proteasome system (UPS) degrades point mutants that retain partial function but have decreased stability compared to their wild-type counterparts. This class of UPS substrate includes routine translational errors and numerous human disease alleles, such as the most common cause of cystic fibrosis, ΔF508-CFTR. Yet, there is no systematic way to discover novel examples of these "minimally misfolded" substrates. To address that shortcoming, we designed a genetic screen to isolate func...
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Matthew P Flagg Breanna Lam Darren K Lam Tiffany M Le Andy Kao Yousif I Slaiwa Randolph Y Hampton Source Type: research
CRISPR-mediated reversion of oncogenic KRAS mutation results in increased proliferation and reveals independent roles of Ras and mTORC2 in the migration of A549 lung cancer cells
Mol Biol Cell. 2023 Sep 20:mbcE23050152. doi: 10.1091/mbc.E23-05-0152. Online ahead of print.ABSTRACTAlthough the RAS oncogene has been extensively studied, new aspects concerning its role and regulation in normal biology and cancer continue to be discovered. Recently, others and we have shown that the mechanistic Target of Rapamycin Complex 2 (mTORC2) is a Ras effector in Dictyostelium and mammalian cells. mTORC2 plays evolutionarily conserved roles in cell survival and migration and has been linked to tumorigenesis. Since RAS is often mutated in lung cancer, we investigated whether a Ras-mTORC2 pathway contributes to enh...
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Alyssa N Werner Avani I Kumar Pascale G Charest Source Type: research
The C-terminus of α-Synuclein regulates its dynamic cellular internalization by Neurexin 1β
Mol Biol Cell. 2023 Sep 20:mbcE22110496. doi: 10.1091/mbc.E22-11-0496. Online ahead of print.ABSTRACTThe aggregation of the disordered neuronal protein, α-Synuclein (αS), is the primary pathological feature of Parkinson's disease. Current hypotheses favor cell-to-cell spread of αS species as underlying disease progression, driving interest in identifying the molecular species and cellular processes involved in cellular internalization of αS. Prior work from our lab identified the chemically specific interaction between αS and the pre-synaptic adhesion protein neurexin 1β (N1β) to be capable of driving cellular inter...
Source: Mol Biol Cell - September 20, 2023 Category: Molecular Biology Authors: Melissa Birol Isabella Ioana Douzoglou Mu ñoz Elizabeth Rhoades Source Type: research
Green synthesis of (S)-1-(furan-2-yl)propan-1-ol from asymmetric bioreduction of 1-(furan-2-yl)propan-1-one using whole-cell of Lactobacillus paracasei BD101
In this study, the synthesis of (S)-2 was carried out by performing the asymmetric bioreduction of 1-(furan-2-yl)propan-1-one (1) using the Lactobacillus paracasei BD101 biocatalyst obtained from boza, a grain-based fermented beverage. (S)-2 was obtained with >99% conversion, >99% ee, and 96% yield under the optimized conditions. Furthermore, in 50 h, 8.37 g of 1 was entirely transformed into (S)-2 on gram scale (96% isolated yield, 8.11 g). This is the first report on the high-gram scale biocatalyzed synthesis of enantiopure (S)-2. These data suggest that L. paracasei BD101 can be used to bioreduction of 1 in gram s...
Source: Chirality - September 20, 2023 Category: Molecular Biology Authors: Ali Sava ş Bülbül Engin Şahin Source Type: research
The role of miR-433-3p in vascular calcification in type 2 diabetic patients: targeting WNT/ β-Catenin and RANKL/RANK/OPG signaling pathways
CONCLUSION: Collectively, the study documented that down-regulation of miR-433-3p may contribute to the development of VC through activating WNT/β-Catenin and RANKL/RANK/OPG signaling pathways.PMID:37728820 | DOI:10.1007/s11033-023-08792-9
Source: Molecular Biology Reports - September 20, 2023 Category: Molecular Biology Authors: Amira M Elshamy Yasser Mostafa Hafez Mohamed A E Safa Hoda A Ibrahim Mohamed Khalfallah Fatma H Rizk Eman F Eltabaa Muhammad T Abdel Ghafar Marwa Mohamed Atef Source Type: research
