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Pain Research Gene Expression Analysis
Potent and Proven Transfection KitsPain Researchers have successfully modulated 25+ genes involved in pain pathways using our Transfection Kits. Highlights include: DOR,The β3 subunit of  Na+,K+-ATPase, NTS1, NAV1.8, Kv 1.1, Kv 9.1, TROY, NOV, β-arrestin, TRPV1, CAV1.2, TLR4 and ASIC and more!  To learn more, check out our Transfection Kit Publications and Blog.Figures: Intrathecal Kv9.1 siRNA treatment induces pain behaviors in naive rats. A, qRT-PCR quantification of Kv9.1 mRNA in rat PASMC cultures transfected with one of three Kv9.1 siRNA sequences or control siRNA. B, qRT-PCR showing Kv9.1 in vivo knock-do...
Source: Neuromics - July 23, 2014 Category: Neuroscience Tags: chronic pain gene expression analysis Gene Silencing i-Fect inflammatory nociception Nociceptin Pain Research siRNA delivery in-vivo Source Type: news

Efficient in vitro gene therapy with PEG siRNA lipid nanocapsules for passive targeting strategy in melanoma.
The objective of this work consists in formulating and optimizing the encapsulation of siRNA into lipid nanocapsules (LNCs) for efficient gene therapy on melanoma cells. SiRNA LNCs were prepared from DOTAP/DOPE lipoplexes, and the siRNA dose and lipid/siRNA charge ratio were assayed to improve the stability and encapsulation yield. Cryo-TEM imaging of the siRNA lipoplexes and LNC morphology revealed a specific organization of the siRNA DOTAP/DOPE lipoplexes as well as specific lipid microstructures. No cytotoxicity of the siRNA LNCs against the melanoma SK-Mel28 cell line was observed at concentrations of up to 500 ng/mL s...
Source: Biotechnology Journal - September 26, 2014 Category: Biotechnology Authors: Resnier P, LeQuinio P, Lautram N, André E, Gaillard C, Bastiat G, Benoit JP, Passirani C Tags: Biotechnol J Source Type: research

siRNA blocking the RAS signalling pathway and inhibits the growth of oesophageal squamous cell carcinoma in nude mice
The aim of this study was to study RAS‐siRNA blocking RAS pathway and suppressing cell growth in human oesophageal squamous cell carcinoma in nude mice. The methods in this study was to construct RAS‐siRNA expression vector, establish 40 oesophageal squamous cell carcinoma xenograft animal models and divided them into five groups: control group, siRNA control group, RAS‐siRNA group, paclitaxel group and RAS‐siRNA and paclitaxel group. We observed tumour growth in nude mice, studied histology by HE staining, tumour growth inhibition by TUNEL assay and detected the RAS, MAPK and cyclin D1 protein expression by immuno...
Source: Cell Biochemistry and Function - November 28, 2014 Category: Biochemistry Authors: Xinjie Wang, Yuling Zheng, Qingxia Fan, Xudong Zhang, Yonggang Shi Tags: Research Article Source Type: research

Transcutaneous iontophoretic delivery of STAT3 siRNA using layer-by-layer chitosan coated gold nanoparticles to treat melanoma
In conclusion, layer-by-layer chitosan coated AuNP can be developed as a carrier for iontophoretic delivery of STAT3 siRNA to treat melanoma. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - June 16, 2016 Category: Biochemistry Source Type: research

Transcutaneous iontophoretic delivery of STAT3 siRNA using layer-by-layer chitosan coated gold nanoparticles to treat melanoma.
In conclusion, layer-by-layer chitosan coated AuNP can be developed as a carrier for iontophoretic delivery of STAT3 siRNA to treat melanoma. PMID: 27318964 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - June 2, 2016 Category: Biotechnology Authors: Labala S, Jose A, Venuganti VV Tags: Colloids Surf B Biointerfaces Source Type: research

Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention against head and neck cancer-containing HPV16 cell lines.
Conclusion: Our results indicate that siRNA E6 and/or E7 may have potential as a gene-specific therapy for human papillomavirus (HPV) type 16 (HPV16)-related squamous cell carcinoma of the head and neck (HNSCC). Objectives: To evaluate the effectiveness of siRNA targeting E6 and/or E7 on the in vitro and in vivo growth suppression of HPV16-related HNSCC. Methods: HPV16-related HNSCC (UM-SCC47) cell lines were used for the present study. Expression of HPV viral oncogenes E6 and/or E7 and their cellular targets, p53 and pRb, was evaluated by real-time PCR, Western blotting, and immunofluorescence staining. To study the effec...
Source: Acta Oto-Laryngologica - May 3, 2013 Category: ENT & OMF Authors: Adhim Z, Otsuki N, Kitamoto J, Morishita N, Kawabata M, Shirakawa T, Nibu KI Tags: Acta Otolaryngol Source Type: research

Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors  for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Nanocarriers for Sirna Delivery Aimed at the Treatment of Melanoma: Systematic Review
CONCLUSION: Such being the case, the development of these systems becomes a non-invasive and promising option for the treatment of melanoma.PMID:37170995 | DOI:10.2174/1567201820666230425234700
Source: Current Drug Delivery - May 12, 2023 Category: Drugs & Pharmacology Authors: Brenda Regina de Araujo Tatielle do Nascimento Ana Paula Dos Santos Matos Vanessa Brand ão de Souza Belmiro Mariana Sato de Souza de Bustamante Monteiro Ralph Santos-Oliveira Eduardo Ricci-Junior Source Type: research

Non-targeting siRNA-mediated responses are associated with apoptosis in chemotherapy-induced senescent skin cancer cells
Chem Biol Interact. 2022 Nov 4:110254. doi: 10.1016/j.cbi.2022.110254. Online ahead of print.ABSTRACTIt is widely accepted that siRNA transfection can promote some off-target effects in the genome; however, little is known about how the cells can respond to the presence of non-viral dsRNA. In the present study, non-targeting control siRNA (NTC-siRNA) was used to evaluate its effects on the activity of pathogen and host-derived nucleic acid-associated signaling pathways such as cGAS-STING, RIG-I, MDA5 and NF-κB in A431 skin cancer cells and BJ fibroblasts. NTC-siRNA treatment promoted cytotoxicity in cancer cells. Furtherm...
Source: Chemico-Biological Interactions - November 7, 2022 Category: Molecular Biology Authors: Gabriela Betlej Dominika B łoniarz Anna Lewi ńska Maciej Wnuk Source Type: research

Inhibition of skin squamous cell carcinoma proliferation and promote apoptosis by dual silencing of NET-1 and survivin.
Authors: Ji ZJ, Wang JL, Chen L Abstract The simultaneous silencing of multiple upregulated genes is an attractive and viable strategy to treat many incurable diseases including cancer. In the present study, skin squamous cell carcinoma (SSCC) tissue microarray was constructed and the expression of NET-1 and survivin was identified. The high expression of NET-1 and survivin gene in SSCC was confirmed as an important event for the formation and development of the cancer. A total of 100 primary SSCC patients were included in the present study. Expression of NET-1 and survivin in cancer cells was evaluated immunohist...
Source: Oncology Reports - June 20, 2015 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

STAT3-siRNA induced B16.F10 melanoma cell death: Association with VEGF downregulation than p-STAT3 knockdown
Publication date: Available online 30 May 2018 Source:Saudi Pharmaceutical Journal Author(s): Aws Alshamsan STAT3 knockdown by small interfering RNA (siRNA) has been described to inhibit carcinogenic growth in various types of tumors. Earlier we have reported delivery of siRNA by oleic acid- and stearic acid-modified-polyethylenimine and enhancement of silencing of STAT3 by small interfering RNA (siRNA) in B16.F10 melanoma cell lines and consequent tumor suppression. Present investigation mainly focused on the downstream events involved in B16.F10 melanoma cell death and consequent tumor suppression following knockdown of...
Source: Saudi Pharmaceutical Journal - May 31, 2018 Category: Drugs & Pharmacology Source Type: research