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Identification and Analysis of Primary Cilia in the Corneal Endothelial Cells of Patients with Bullous Keratopathy
CONCLUSION: The findings in this study clearly demonstrate the expression of primary cilia in the CECs of patients afflicted with BK.PMID:37706487 | DOI:10.1080/02713683.2023.2259633
Source: Current Eye Research - September 14, 2023 Category: Opthalmology Authors: Hideto Deguchi Hidetoshi Tanioka Mako Watanabe Noriko Horiuchi Hideki Fukuoka Osamu Hieda Tsutomu Inatomi Shigeru Kinoshita Chie Sotozono Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Identification and Analysis of Primary Cilia in the Corneal Endothelial Cells of Patients with Bullous Keratopathy
CONCLUSION: The findings in this study clearly demonstrate the expression of primary cilia in the CECs of patients afflicted with BK.PMID:37706487 | DOI:10.1080/02713683.2023.2259633
Source: Current Eye Research - September 14, 2023 Category: Opthalmology Authors: Hideto Deguchi Hidetoshi Tanioka Mako Watanabe Noriko Horiuchi Hideki Fukuoka Osamu Hieda Tsutomu Inatomi Shigeru Kinoshita Chie Sotozono Source Type: research
Navigating carbon emissions in G-7 economies: a quantile regression analysis of environmental-economic interplay
This study employs panel data from 1990 to 2020 for the G-7 countries (Canada, France, Germany, Italy, Japan, the UK, and the USA) and employs the examination of heterogeneous slope coefficients and cross-sectional dependence tests as preliminary steps before conducting cointegration analysis and second-generation unit-root tests. This study employs the method of movement quantile regression (MMQR) to analyze long-run and short-run relationships. The findings from the MMQR model indicate that economic growth and imports have a negative impact on consumption-based CO2 (CCO2) emissions, which worsens at higher quantiles. On ...
Source: Environmental Science and Pollution Research International - September 14, 2023 Category: Environmental Health Authors: Bowen Luo Arshad Ahmad Khan Xiaoming Wu Hongxia Li Source Type: research
Identification and Analysis of Primary Cilia in the Corneal Endothelial Cells of Patients with Bullous Keratopathy
CONCLUSION: The findings in this study clearly demonstrate the expression of primary cilia in the CECs of patients afflicted with BK.PMID:37706487 | DOI:10.1080/02713683.2023.2259633
Source: Current Eye Research - September 14, 2023 Category: Opthalmology Authors: Hideto Deguchi Hidetoshi Tanioka Mako Watanabe Noriko Horiuchi Hideki Fukuoka Osamu Hieda Tsutomu Inatomi Shigeru Kinoshita Chie Sotozono Source Type: research
Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research
Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research
Incidence of Hypophosphatemia After Intravenous Administration of Iron: A Matching-Adjusted Indirect Comparison of Data from Japanese Randomized Controlled Trials
CONCLUSIONS: Direct comparison of patient-level data and a MAIC from two RCTs in Japanese patients with heavy menstrual bleeding indicated that hypophosphatemia is less frequent in patients treated with FDI than those with FCM or SFO. Results are in agreement with RCTs comparing FDI and FCM in patients with various etiologies conducted in the USA and Europe.PMID:37702931 | DOI:10.1007/s12325-023-02591-1
Source: Adv Data - September 13, 2023 Category: Epidemiology Authors: Seiji Fukumoto Tatsunori Murata Yutaka Osuga Richard F Pollock Source Type: research
