Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Recent development of affinity-based assay systems
Anal Sci. 2023 Oct;39(10):1613-1614. doi: 10.1007/s44211-023-00410-9.NO ABSTRACTPMID:37749357 | DOI:10.1007/s44211-023-00410-9 (Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry)
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 25, 2023 Category: Chemistry Authors: Ken-Ichi Ohno Source Type: research

Highly sensitive electrochemical detection of cholesterol based on Au-Pt NPs/PAMAM-ZIF-67 nanomaterials
Anal Sci. 2023 Sep 25. doi: 10.1007/s44211-023-00427-0. Online ahead of print.ABSTRACTA cholesterol biosensor was constructed by bimetallic (Au and Pt) and poly(amidoamine)-zeolite imidazole framework (PAMAM-ZIF-67). First, PAMAM-ZIF-67 nanomaterial was immobilized onto the electrode, and then Au and Pt were modified on the electrode by the electro-deposition method. Subsequently, cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) were fixed on the electrode. The stepwise modification procedures were recorded by impedance spectroscopy and voltammetry. The current response presented a linear relation to the logarith...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 25, 2023 Category: Chemistry Authors: Liangtian Zhang Jianmeng Zhu Wenzhong Hong Gang Li Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research

Digital PCR using a simple PDMS microfluidic chip and standard laboratory equipment
Anal Sci. 2023 Sep 14. doi: 10.1007/s44211-023-00425-2. Online ahead of print.ABSTRACTDigital PCR (dPCR) enables sensitive and precise quantification of template nucleic acid without calibration. However, dPCR is not yet in widespread use, probably due to the need for expensive specialized instruments. In this paper, we describe a dPCR system using a simple microfluidic chip and common laboratory tools. The microfluidic chip consists of two parts: a PDMS part with 24,840 × 0.25 nL microwells and a PDMS-coated flat glass plate. Human RNase P gene was adopted as the model template. Commercial products of human genomic DNA a...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Kazuo Hosokawa Hitoshi Ohmori Source Type: research

Pentafluorobenzylpyridinium: new thermometer ion for characterizing the ions produced by collisional activation during tandem mass spectrometry
In this study, pentafluorobenzylpyridinium (F5-BnPy+), which has the highest dissociation energy among the reported benzylpyridinium thermometer ion, is proposed to characterize the internal energy distributions of ions activated by higher energy collisional dissociation (HCD) and ion-trap collision-induced dissociation (CID) during tandem mass spectrometry. The dissociation threshold energies of F5-BnPy+ was determined using quantum chemistry calculations at the CCSD(T)/6-311++G(d,p)//M06-2X-D3/6-311++G(d,p) level of theory, and the appearance energies for ion dissociation in HCD and ion-trap CID were estimated using Rice...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - September 14, 2023 Category: Chemistry Authors: Daiki Asakawa Kazumi Saikusa Source Type: research