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Condition: Pregnancy

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Total 418 results found since Jan 2013.

Gonadotropin-Releasing Hormone Type II (GnRH-II) Agonist Regulates the Motility of Human Decidual Endometrial Stromal Cells: Possible Effect on Embryo Implantation and Pregnancy.
Abstract Invasion of the maternal decidua by extravillous trophoblast is an important process for embryo implantation and placentation in human. Motile behavior of decidual endometrial stromal cells have been considered of critical importance for embryo implantation and programming of human pregnancy. The GnRH effects in endometrium have raised concerns in reproduction. In the present study, we examined the action of GnRH-II agonist-promoted motility of human decidual endometrial stromal cells and the mechanisms of the action, indicating the role of GnRH-II agonist in embryo implantation and early pregnancy. Human...
Source: Biology of Reproduction - March 11, 2015 Category: Reproduction Medicine Authors: Wu HM, Huang HY, Lee CL, Soong YK, Leung PC, Wang HS Tags: Biol Reprod Source Type: research

Differential expression and regulation of Runx1 in mouse uterus during the peri-implantation period
Abstract Runx1 transcription factor is a key developmental regulator. However, little is known about the effects of Runx1 on embryo implantation and decidualization. The aim of this study is to examine the expression and regulation of Runx1 in mouse uterus during the peri-implantation period. There was no evident Runx1 mRNA signal on days 1–4 of pregnancy. On day 5 of pregnancy, Runx1 mRNA was mainly localized in the subluminal stroma surrounding the implanting blastocyst. A similar result was observed in the estrogen-activated implantation uterus. Simultaneously, a high level of Runx1 mRNA expression was detect...
Source: Cell and Tissue Research - April 20, 2015 Category: Cytology Source Type: research

Fatty acid binding protein 7 and N‐3 poly unsaturated fatty acid supply in early rat brain development
This article is protected by copyright. All rights reserved.
Source: Journal of Neurobiology - June 1, 2015 Category: Neurology Authors: Elise Maximin, Bénédicte Langelier, Josiane Aïoun, Kaïs H. Al‐Gubory, Christian Bordat, Monique Lavialle, Christine Heberden Tags: Research Article Source Type: research

Tumor necrosis factor superfamily member 15 (Tnfsf15) facilitates lymphangiogenesis via upregulation of Vegfr3 gene expression in lymphatic endothelial cells
We report here that Tnfsf15, which is known to induce apoptosis in vascular endothelial cells, can promote lymphatic endothelial cell (LEC) growth and migration, stimulate lymphangiogenesis, and facilitate lymphatic circulation. Treatment of mouse LEC with Tnfsf15results in upregulation of Vegfr3 expression; this can be inhibited by gene‐silencing of death domain–containing receptor‐3 (DR3; Tnfrsf25), a cell surface receptor for Tnfsf15, with siRNA, or by blocking Tnfsf15‐DR3 interaction with a Tnfsf15 neutralizing antibody 4‐3H. Additionally, Tnfsf15/DR3 signalling pathways in LEC include activation of NF‐κB....
Source: The Journal of Pathology - June 22, 2015 Category: Pathology Authors: Ting‐Ting Qin, Guo‐Ce Xu, Jian‐Wei Qi, Gui‐Li Yang, Kun Zhang, Hai‐Lin Liu, Li‐Xia Xu, Rong Xiang, Guozhi Xiao, Huiling Cao, Yuquan Wei, Qiang‐Zhe Zhang, Lu‐Yuan Li Tags: Original Paper Source Type: research

The Role of Endometrial Selectins and Their Ligands on Bovine Conceptus Attachment to the Uterine Epithelium During Peri-Implantation Period.
The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the peri-attachment period, and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-seq analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0 = day of estrus), only the L-selectin (SELL) ligand, podocalyxin (PODXL) and P-selectin (SELP) ligand, SELPLG were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epith...
Source: Biology of Reproduction - July 1, 2015 Category: Reproduction Medicine Authors: Bai R, Kusama K, Sakurai T, Bai H, Wang C, Zhang J, Kuse M, Ideta A, Aoyagi Y, Okuda K, Imakawa K Tags: Biol Reprod Source Type: research

Inhibition of DDAH1, but not DDAH2, results in apoptosis of a human trophoblast cell line in response to TRAIL
This study used the human extravillous trophoblast-derived cell line SGHPL-4 cells. All experiments were performed at least three times. PARTICIPANTS/MATERIALS, SETTING, METHODS The effect of DDAH on trophoblast apoptosis was examined using siRNA and time-lapse microscopy. Changes in the expression of DDAH were followed by PCR and western blot analysis. Receptor expression was followed by flow cytometry. MAIN RESULTS AND THE ROLE OF CHANCE Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis induci...
Source: Human Reproduction - July 19, 2015 Category: Reproduction Medicine Authors: Lumicisi, B. A., Cartwright, J. E., Leslie, K., Wallace, A. E., Whitley, G. S. Tags: Early pregnancy Source Type: research

Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo
Abstract The continual proliferation and differentiation of trophoblasts are critical for the maintenance of pregnancy. It is well known that the tissue stem cells are associated with the development of tissues and pathologies. It has been demonstrated that side-population (SP) cells identified by fluorescence-activated cell sorting (FACS) are enriched with stem cells. The SP cells in HTR-8/SVneo cells derived from human primary trophoblast cells were isolated by FACS. HTR-8/SVneo-SP cell cultures generated both SP and non-SP (NSP) subpopulations. In contrast, NSP cell cultures produced NSP cells and failed to pro...
Source: Human Cell - July 30, 2015 Category: Cytology Source Type: research

The expression of the imprinted gene PHLDA2 in placental tissues of preeclampsia and its effects on the proliferation, migration and invasion of trophoblast cells JEG-3.
This article is protected by copyright. All rights reserved. PMID: 26218012 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Pharmacology and Physiology - July 28, 2015 Category: Drugs & Pharmacology Authors: Jin F, Qiao C, Luan N, Shang T Tags: Clin Exp Pharmacol Physiol Source Type: research

Tumour necrosis factor superfamily member 15 (Tnfsf15) facilitates lymphangiogenesis via up‐regulation of Vegfr3 gene expression in lymphatic endothelial cells
We report here that Tnfsf15, which is known to induce apoptosis in vascular endothelial cells, can promote lymphatic endothelial cell (LEC) growth and migration, stimulate lymphangiogenesis, and facilitate lymphatic circulation. Treatment of mouse LECs with Tnfsf15 results in up‐regulation of Vegfr3 expression; this can be inhibited by gene silencing of death domain‐containing receptor‐3 (DR3; Tnfrsf25), a cell surface receptor for Tnfsf15, with siRNA, or by blocking Tnfsf15–DR3 interaction with a Tnfsf15 neutralizing antibody, 4‐3H. Additionally, Tnfsf15/DR3 signalling pathways in LECs include activation of NF...
Source: The Journal of Pathology - August 6, 2015 Category: Pathology Authors: Ting‐Ting Qin, Guo‐Ce Xu, Jian‐Wei Qi, Gui‐Li Yang, Kun Zhang, Hai‐Lin Liu, Li‐Xia Xu, Rong Xiang, Guozhi Xiao, Huiling Cao, Yuquan Wei, Qiang‐Zhe Zhang, Lu‐Yuan Li Tags: Original Paper Source Type: research

2,3,7,8-Tetrachlorodibenzo-p-Dioxin Differentially Suppresses Angiogenic Responses in Human Placental Vein and Artery Endothelial Cells.
Abstract Placental angiogenesis is dramatically increased during pregnancy in association with the elevated placental blood flows to support the rapidly growing fetus. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an environmental toxicant and a ligand of aryl hydrocarbon receptor (AhR). Herein, we investigated the effects of TCDD on proliferation, migration, and viability of fetoplacental endothelial cells in response to a complete growth medium which contained serum and growth supplement using human umbilical cord vein (HUVECs) and artery (HUAECs) cells as models. We found that TCDD dose- and time-dependently in...
Source: Toxicology - August 11, 2015 Category: Toxicology Authors: Li Y, Wang K, Zou QY, Magness RR, Zheng J Tags: Toxicology Source Type: research

Galectin-Protozoan Interactions in Subverting Immunity Molecular Bases of Disease
Trichomoniasis is the most common non-viral sexually transmitted infection caused by the vaginotropic extracellular protozoan parasite Trichomonas vaginalis. The infection is recurrent, with no lasting immunity, often asymptomatic, and linked to pregnancy complications and risk of viral infection. The molecular mechanisms of immune evasion by the parasite are poorly understood. We demonstrate that galectin-1 and -3 are expressed by the human cervical and vaginal epithelial cells and act as pathogen-recognition receptors for the ceramide phosphoinositol glycan core (CPI-GC) of the dominant surface protozoan lipophosphoglyca...
Source: Journal of Biological Chemistry - January 8, 2016 Category: Chemistry Authors: Fichorova, R. N., Yamamoto, H. S., Fashemi, T., Foley, E., Ryan, S., Beatty, N., Dawood, H., Hayes, G. R., St-Pierre, G., Sato, S., Singh, B. N. Tags: Immunology Source Type: research

Increased Expression of Fatty Acid Binding Protein 4 in Preeclamptic Placenta and Its Relevance to Preeclampsia
The aim of this investigation was to determine the expression of fatty acid binding protein 4 (FABP4) in the placenta from women with preeclampsia and normal pregnancy, and to delineate the regulatory effects on thophoblast cell by FABP4. We determined the expression of FABP4 by real-time polymerase chain reaction (PCR) for messenger ribonucleic acid (mRNA) or enzyme-linked immunesorbent assay (ELISA) and Western blotting for protein. Small interference of ribonucleic acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4.
Source: Placenta - January 19, 2016 Category: Reproduction Medicine Authors: Yuying Yan, Huilian Peng, Peng Wang, Hanzhi Wang, Minyue Dong Source Type: research

TNF-α induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA in Kawasaki disease model.
In this study, firstly we verified the overexpressed TNF-α in KD patients, and found TNF-α was able to induce HUVECs apoptosis and inhibit HUVECs proliferation. After this we screened out pregnancy induced noncoding RNA (PINC) was significantly overexpression in TNF-α treated HUVECs. We also found PINC overexpressed in KD patients. For further study, we designed two siRNA of PINC. After silenced the expression of PINC in HUVECs, we found the Knockdown of PINC enhanced the viability of HUVECs treated with TNF-α, and increased the expression of anti-apoptotic and reduced the expression of apoptotic gene. These results su...
Source: The International Journal of Biochemistry and Cell Biology - January 18, 2016 Category: Biochemistry Authors: Jiang C, Fang X, Jiang Y, Shen F, Hu Z, Li X, Huang X Tags: Int J Biochem Cell Biol Source Type: research

The nuclear receptor REV‐ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells
Abstract Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor (Gdf)10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells (UESCs) isolated from pregnant Per2‐dLuc transgenic rats. ...
Source: Physiological Reports - January 26, 2016 Category: Physiology Authors: Lijia Zhao, Keishiro Isayama, Huatao Chen, Nobuhiko Yamauchi, Yasufumi Shigeyoshi, Seiichi Hashimoto, Masa‐aki Hattori Tags: Original Research Source Type: research

The nuclear receptor REV-ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells.
Abstract Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor (Gdf)10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells (UESCs) isolated from pregnant Per2-dLuc transgenic...
Source: Physiological Reviews - January 28, 2016 Category: Physiology Authors: Zhao L, Isayama K, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA Tags: Physiol Rep Source Type: research