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Specialty: Molecular Biology
Condition: Osteoarthritis

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Total 48 results found since Jan 2013.

Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression.
Authors: Zhang C, Yu W, Huang C, Ding Q, Liang C, Wang L, Hou Z, Zhang Z Abstract High‑mobility group box chromosomal protein (HMGB‑1) contributes to osteoarthritis (OA) by modulating various oxidative, inflammatory and apoptotic signaling pathways. The effect of chrysin (CH), a natural plant flavonoid, and its functional interaction with HMGB‑1, was investigated in a chondrocyte model of OA. Human chondrocytes were pre‑treated with CH, and then subsequently treated with IL‑1β to induce the formation of chondrocytes similar to those found in OA joints. Next, the expression level of HMGB‑1 was determine...
Source: Molecular Medicine Reports - December 12, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Inhibition of YAP with siRNA prevents cartilage degradation and ameliorates osteoarthritis development
AbstractThe Hippo/YAP signaling pathway is important for mediating organ size and tissue homeostasis, but its role in osteoarthritis (OA) remains unclear. We aimed to investigate the role of Hippo/YAP signaling pathway in OA development. YAP expression in OA cartilage was assessed by immunohistochemistry, RT-qPCR, and Western blotting. The effects of YAP overexpression or knockdown on gene expression related to chondrocyte hypertrophy induced by IL-1 β were examined. The in vivo effects of YAP inhibition were studied. Subchondral bone was analyzed by micro-CT. YAP was increased in mice and human OA articular cartilage and...
Source: Journal of Molecular Medicine - November 21, 2018 Category: Molecular Biology Source Type: research

Inhibition of C5a prevents IL-1β-induced alternations in rat synoviocytes in vitro
Publication date: Available online 6 August 2018Source: Molecular and Cellular ProbesAuthor(s): Wei Lu, Lin Wang, Jing Yao, Wen Wang, Yu ChenAbstractC5a is an important pro-inflammatory peptide involved in complement activation, membrane attack complex formation, immune cell chemotaxis, and allergic responses. Osteoarthritis is a disease characterized by degenerative changes in articular cartilage. It has recently been found that inflammatory responses play an important role in the pathogenesis of osteoarthritis and also in rheumatoid arthritis, where dysfunctional synoviocytes are involved. We performed a series of studie...
Source: Molecular and Cellular Probes - August 6, 2018 Category: Molecular Biology Source Type: research

Role of IFT88 in icariin ‑regulated maintenance of the chondrocyte phenotype.
Role of IFT88 in icariin‑regulated maintenance of the chondrocyte phenotype. Mol Med Rep. 2018 Jan 25;: Authors: Xiang W, Zhang J, Wang R, Wang L, Wang S, Wu Y, Dong Y, Guo F, Xu T Abstract Maintenance of the chondrocyte phenotype is crucial for cartilage repair during tissue engineering. Intraflagellar transport protein 88 (IFT88) is an essential component of primary cilia, shuttling signals along the axoneme. The hypothesis of the present study was that IFT88 could exert an important role in icariin‑regulated maintenance of the chondrocyte phenotype. To this end, the effects of icariin on prolife...
Source: Molecular Medicine Reports - February 4, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Antagonism of cysteinyl leukotriene receptor 1 (cysLTR1) by montelukast suppresses cell senescence of chondrocytes.
Abstract Aging is closely associated with osteoarthritis (OA). Although its underlying mechanisms remain unknown, cellular senescence in chondrocytes has become an important therapeutic target for the treatment of OA. Cysteinyl leukotriene receptors (cysLTRs) mediate the pathobiological function of cysteinyl leukotrienes (cysLTs). However, the roles of cysLTRs in the pathogenesis of OA have not been reported before. In the current study, we found that cysLTR1 but not cysLTR2 is expressed in human primary chondrocytes. In addition, stimulation with tumor necrosis factor α (TNF-α) resulted in a significant increas...
Source: Cytokine - January 10, 2018 Category: Molecular Biology Authors: Song W, Zhang Y, Wang J, Ma T, Hao L, Wang K Tags: Cytokine Source Type: research

Oxidized low density lipoprotein facilitates tumor necrosis factor ‑α mediated chondrocyte death via autophagy pathway.
Oxidized low density lipoprotein facilitates tumor necrosis factor‑α mediated chondrocyte death via autophagy pathway. Mol Med Rep. 2017 Oct 13;: Authors: Shen P, Zhu Y, Zhu L, Weng F, Li X, Xu Y Abstract We aimed to investigate the role of oxidized low density lipoprotein (ox‑LDL) in tumor necrosis factor‑α (TNF‑α) mediated chondrocyte death and explore the mechanisms. Ten osteoarthritis (OA) and normal control cartilage tissue and synovial fluid (SF) samples were collected, and the expression of lectin‑like ox‑LDL receptor‑1 (LOX‑1) and ox‑LDL level was examined by real time quan...
Source: Molecular Medicine Reports - October 20, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Underlying mechanism of Sirt1 on apoptosis and extracellular matrix degradation of osteoarthritis chondrocytes.
In conclusion, upregulation of Sirt1 expression may inhibit OA chondrocyte apoptosis and extracellular matrix degradation by increasing Bcl‑2 expression and decreasing Bax, MMP1 and MMP13 expression, via downregulation of p38, JNK and ERK phosphorylation. PMID: 28586000 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - June 8, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Therapeutic mechanisms of ibuprofen, prednisone and betamethasone in osteoarthritis.
In conclusion, prednisone, ibuprofen and betamethasone may prevent OA by suppressing the expression of IL‑6 and IL‑8, subsequently inactivating NF‑κB and STAT3 pathways, and ultimately, leading to decreased levels of collagen I, MMP‑1, and MMP‑13. PMID: 28035387 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - January 1, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Role of protein arginine methyltransferase 5 in inflammation and migration of fibroblast-like synoviocytes in rheumatoid arthritis.
Abstract To probe the role of protein arginine methyltransferase 5 (PRMT5) in regulating inflammation, cell proliferation, migration and invasion of fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). FLSs were separated from synovial tissues (STs) from patients with RA and osteoarthritis (OA). An inhibitor of PRMT5 (EPZ015666) and short interference RNA (siRNA) against PRMT5 were used to inhibit PRMT5 expression. The standard of protein was measured by Western blot or immunofluorescence. The excretion and genetic expression of inflammatory factors were, respectively, estimated by enz...
Source: J Cell Mol Med - November 16, 2016 Category: Molecular Biology Authors: Chen D, Zeng S, Huang M, Xu H, Liang L, Yang X Tags: J Cell Mol Med Source Type: research

Role of protein arginine methyltransferase 5 in inflammation and migration of fibroblast ‐like synoviocytes in rheumatoid arthritis
Abstract To probe the role of protein arginine methyltransferase 5 (PRMT5) in regulating inflammation, cell proliferation, migration and invasion of fibroblast‐like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). FLSs were separated from synovial tissues (STs) from patients with RA and osteoarthritis (OA). An inhibitor of PRMT5 (EPZ015666) and short interference RNA (siRNA) against PRMT5 were used to inhibit PRMT5 expression. The standard of protein was measured by Western blot or immunofluorescence. The excretion and genetic expression of inflammatory factors were, respectively, estimated by enzyme‐l...
Source: Journal of Cellular and Molecular Medicine - October 31, 2016 Category: Molecular Biology Authors: Dongying Chen, Shan Zeng, Mingcheng Huang, Hanshi Xu, Liuqin Liang, Xiuyan Yang Tags: Original Article Source Type: research

Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function
This study represents the first functional study of Fis1 with its pathological relevance to OA. Our data suggest a new target for controlling cartilage-degenerative diseases, such as OA.Key messageFis1 suppression in OA chondrocytes induces accumulation and inhibition of lysosomes.Fis1 suppression alters miRNAs, especially those implicated in lysosomal regulation.Lysosomal destruction results in chondrocyte apoptosis and suppression of autophagy.Fis1 depletion in zebrafish causes lysosome accumulation, mitochondrial dysfunction, and peroxisome reduction.This is the first functional study of Fis1 and its pathological relevance to OA.
Source: Journal of Molecular Medicine - August 5, 2016 Category: Molecular Biology Source Type: research

hsa-miR-15a exerts protective effects against osteoarthritis by targeting aggrecanase-2 (ADAMTS5) in human chondrocytes.
Abstract The aim of the present study was to examine the expression levels and role of hsa-miR-15a in osteoarthritis (OA), as well as the associated mechanisms. The expression levels of hsa-miR-15a and A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (ADAMTS5, also known as aggrecanase-2) were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in both OA and normal chondrocytes. hsa-miR‑21 mimics or antisense oligonucleotides (ASO) were co-transfected into the chondrocytes to examine the effects on the putative binding sites compa...
Source: International Journal of Molecular Medicine - December 23, 2015 Category: Molecular Biology Authors: Lu X, Lin J, Jin J, Qian W, Weng X Tags: Int J Mol Med Source Type: research

Interleukin-33 acts as a transcriptional repressor and extracellular cytokine in fibroblast-like synoviocytes in patients with rheumatoid arthritis.
Abstract The present study aimed to assess the functions of interleukin (IL)-33 in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). Enzyme-linked immunosorbent assays (ELISAs) were used to quantify interleukin (IL)-33 in plasma obtained from patients with RA and osteoarthritis (OA). To evaluate functions of intracellular IL-33, levels of inflammatory mediators and matric metalloproteinases (MMPs) were measured in RA FLS transfected with IL-33 small- interfering RNA (siRNA) or plasmids, and changes in the expression and regulation of nuclear factor kappaB (NF-κB) were determined usi...
Source: Cytokine - October 29, 2015 Category: Molecular Biology Authors: Lee EJ, So MW, Hong S, Kim YG, Yoo B, Lee CK Tags: Cytokine Source Type: research

Expression of PADI4 in patients with ankylosing spondylitis and its role in mediating the effects of TNF-α on the proliferation and osteogenic differentiation of human mesenchymal stem cells.
In conclusion, the findings of this study demonstrate that the expression of PADI4 differs between patients with AS and normal subjects. In addition, our data suggest that PADI4 plays a role in hMSC proliferation and differentiation, which are induced by TNF-α. PMID: 26082376 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - June 16, 2015 Category: Molecular Biology Authors: Yang Y, Dai M Tags: Int J Mol Med Source Type: research

Integrin-β1 regulates chondrocyte proliferation and apoptosis through the upregulation of GIT1 expression.
Abstract Chondrocytes play a critical role in the repair process of osteoarthritis, which is also known as degenerative arthritis. Integrins, as the key family of cell surface receptors, are responsible for the regulation of chondrocyte proliferation, differentiation, survival and apoptosis through the recruitment and activation of downstream adaptor proteins. Moreover, G-protein‑coupled receptor kinase interacting protein-1 (GIT1) exerts its effects on cell proliferation and migration through interaction with various cytokines. It has been previously suggested that GIT1 acts as a vital protein downstream of th...
Source: International Journal of Molecular Medicine - February 26, 2015 Category: Molecular Biology Authors: Zhang LQ, Zhao GZ, Xu XY, Fang J, Chen JM, Li JW, Gao XJ, Hao LJ, Chen YZ Tags: Int J Mol Med Source Type: research