hsa-miR-15a exerts protective effects against osteoarthritis by targeting aggrecanase-2 (ADAMTS5) in human chondrocytes.

hsa-miR-15a exerts protective effects against osteoarthritis by targeting aggrecanase-2 (ADAMTS5) in human chondrocytes. Int J Mol Med. 2015 Dec 23; Authors: Lu X, Lin J, Jin J, Qian W, Weng X Abstract The aim of the present study was to examine the expression levels and role of hsa-miR-15a in osteoarthritis (OA), as well as the associated mechanisms. The expression levels of hsa-miR-15a and A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (ADAMTS5, also known as aggrecanase-2) were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in both OA and normal chondrocytes. hsa-miR‑21 mimics or antisense oligonucleotides (ASO) were co-transfected into the chondrocytes to examine the effects on the putative binding sites compared with the negative control (NC)-mimics or NC-ASO. The relative ADAMTS5 mRNA and protein levels were measured by RT-qPCR and western blot anlaysis, respectively. Moreover, after inhibiting the expression of hsa‑miR‑15a and ADAMTS5 by ASO and small interfering RNA (siRNA), respectively, the amounts of proteoglycan and collagen in the cellular matrix and medium were determined. Additionally, the expression levels of collagen II were measured by western blot analysis. hsa‑miR‑15a expression was downregulated, but ADAMTS5 expression was upregulated in the human OA chondrocytes compared to the normal chondrocytes. Luciferase r...
Source: International Journal of Molecular Medicine - Category: Molecular Biology Authors: Tags: Int J Mol Med Source Type: research