Detection of coralyne and heparin by polymerase extension reaction using SYBR Green I
Publication date: Available online 16 July 2019Source: Molecular and Cellular ProbesAuthor(s): Changbei Ma, Miangjian Chen, Hailun He, Leilei ChenAbstractPolydeoxyadenosine (poly (dA)) has been extensively applied for detecting many drug molecules. Herein, we developed a sensitive method for detecting coralyne and heparin using a modified DNA probe with poly (dA) at one end. In the absence of coralyne, the DNA probe was digested by the Exonuclease I (Exo I), and therefore the SYBR Green I (SG I) emitted an extremely low fluorescent signal. While coralyne specifically binding to poly (dA) with strong propensity could remark...
Source: Molecular and Cellular Probes - July 18, 2019 Category: Molecular Biology Source Type: research

Evaluation of two aneuploidy screening tests for chorionic villus samples: Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization
Publication date: Available online 15 July 2019Source: Molecular and Cellular ProbesAuthor(s): Tonghua Wu, Yuanchang Zhu, Ling Hong, Qi Lin, Chunmei Chen, Jing Yang, Lijun Ye, Wensi Huang, Yong ZengAbstractThe vast majority of first-trimester pregnancy losses are the consequence of numerical aberrations in fetal chromosomes, which may involve nearly all chromosomes. Although commercial probes for all chromosomes are available for multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH) analyses, their use has rarely been reported for screening all 24 chromosomes for early fetal ...
Source: Molecular and Cellular Probes - July 16, 2019 Category: Molecular Biology Source Type: research

Quantitative PCR evaluation of deletions/duplications identified by array CGH
Publication date: Available online 11 July 2019Source: Molecular and Cellular ProbesAuthor(s): Federica Baldan, Nadia Passon, Silvia Burra, Eliana Demori, Patrizia Dello Russo, Giuseppe DamanteAbstractGenomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentially clinically relevant imbalances. Only samples with DLRS
Source: Molecular and Cellular Probes - July 12, 2019 Category: Molecular Biology Source Type: research

Application of loop-mediated isothermal amplification (LAMP) for rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus) and haddock (Melanogrammus aeglefinus)
Publication date: Available online 8 July 2019Source: Molecular and Cellular ProbesAuthor(s): Yi Wang, Junli Feng, Xiaolan TianAbstractCodfish is a commercially important species of sea fish and plays an important role in the world fishery. In our study, two loop-mediated isothermal amplification (LAMP) assays (real-time fluorescence LAMP and visual LAMP) were established for the identification of three cod species in Gadidae (Gadus morhua, Gadus macrocephalus and Melanogrammus aeglefinus). 12S rDNA gene was used to design primers to distinguish the Gadidae and non-Gadidae species, and the mitochondrial Cytb gene was selec...
Source: Molecular and Cellular Probes - July 9, 2019 Category: Molecular Biology Source Type: research

SATQPCR: Website for statistical analysis of real-time quantitative PCR data
Publication date: Available online 5 July 2019Source: Molecular and Cellular ProbesAuthor(s): Corinne Rancurel, Trang van Tran, Céline Elie, Frédérique HilliouAbstractSATQPCR is a web tool providing statistical analysis of real-time quantitative PCR data including all MIQE rules (gene efficiency, selection of reference genes and normalization with them).Our application is a quick tool that provides to the biologist, graphs as well as statistical tables summarizing their results with the chosen methods (t-test or ANOVA with Tukey test).The application is available at http://satqpcr.sophia.inra.fr with a...
Source: Molecular and Cellular Probes - July 7, 2019 Category: Molecular Biology Source Type: research

Effects of lycopene on ovarian cancer cell line SKOV3 in vitro: Suppressed proliferation and enhanced apoptosis
This study aims to investigate the influences of lycopene on ovarian cancer cells SKOV3 in vitro and the corresponding mechanism.MethodsSKOV3 cultures were divided into four groups and treated with lycopene at 0 (the control group), 1 × 10−6, 1 × 10−5 and 1 × 10−4 mol/L respectively. The proliferation rate of SKOV3 cells was determined using MTT colorimetric assay, and the apoptosis rate of SKOV3 cells was measured using flow cytometry. Western blotting was used to detect the expressions of Bax and Bcl-2.ResultsWhen compared with the control group, the proliferation rates o...
Source: Molecular and Cellular Probes - July 4, 2019 Category: Molecular Biology Source Type: research

Sp1 promotes cell migration and invasion in oral squamous cell carcinoma by upregulating Annexin A2 transcription
Publication date: Available online 26 June 2019Source: Molecular and Cellular ProbesAuthor(s): Xian-bin Liu, Jing Wang, Ke Li, Xian-nan FanAbstractOral squamous cell carcinoma (OSCC) is a common malignant tumor with high metastatic potential in head and neck. Revealing the mechanism of OSCC metastasis will benefit the prognosis and prevention of OSCC. Sp1 is a transcription factor involved in the progression of several tumors. Annexin A2 functions as an oncogene, and there are three putative Sp1 binding sites in the Annexin A2 promoter region. Therefore, we hypothesized that Sp1 could regulate OSCC metastasis by regulating...
Source: Molecular and Cellular Probes - June 27, 2019 Category: Molecular Biology Source Type: research

The lipid profile of three Malassezia species assessed by Raman spectroscopy and discriminant analysis
Publication date: Available online 25 June 2019Source: Molecular and Cellular ProbesAuthor(s): Chrysoula Petrokilidou, Eleftherios Pavlou, Georgios Gaitanis, Ioannis D. Bassukas, Manolis N. Saridomichelakis, Aristea Velegraki, Nikolaos KourkoumelisAbstractMalassezia yeasts constitute the major eukaryotic cutaneous flora of homoeothermic vertebrates. These lipophilic yeasts are able to cause, trigger, or aggravate common skin diseases under favorable conditions. Species identification and subspecies differentiation is currently based on morphological characteristics, lipid assimilation profile, and molecular tests. Mass spe...
Source: Molecular and Cellular Probes - June 25, 2019 Category: Molecular Biology Source Type: research

Computational design of a chimeric epitope-based vaccine to protect against Staphylococcus aureus infections
Publication date: Available online 22 June 2019Source: Molecular and Cellular ProbesAuthor(s): Nasim Hajighahramani, Mahboobeh Eslami, Manica Negahdaripour, Mohammad Bagher Ghoshoon, Ali Dehshahri, Nasrollah Erfani, Reza Heidari, Ahmad Gholami, Navid Nezafat, Younes GhasemiGraphical abstract (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - June 22, 2019 Category: Molecular Biology Source Type: research

Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation
ConclusionsThe potential effects of VTPLLM + US on apoptosis of MCF-7 cells suggest that applying VTPLLM + US might be an effective strategy in breast cancer therapies. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - June 20, 2019 Category: Molecular Biology Source Type: research

Rapid and visual detection of dengue virus using recombinase polymerase amplification method combined with lateral flow dipstick
Publication date: Available online 14 June 2019Source: Molecular and Cellular ProbesAuthor(s): Yun Xi, Chang-Zhi Xu, Zhi-Zhi Xie, Dong-Lin Zhu, Jie-Ming DongAbstractDengue virus (DENV), a member of the genus Flavivirus within the family Flaviviridae, is one of the most significant mosquito-borne viruses that causing dengue fever in human. A rapid diagnostic would be helpful to detect DENV infection in a timely manner. In the last decade, recombinase polymerase amplification (RPA) technique has been experiencing rapid development and widely employed to detect various other pathogens. In present study, a reverse transcriptio...
Source: Molecular and Cellular Probes - June 14, 2019 Category: Molecular Biology Source Type: research

A PCR-based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological specimens
Publication date: Available online 12 June 2019Source: Molecular and Cellular ProbesAuthor(s): Serafeim C. Chaintoutis, Elpida Papadopoulou, Angeliki Melidou, Anna Papa, Chrysostomos I. DovasAbstractLineage 2 West Nile virus (WNV) strains have been implicated in severe encephalitis outbreaks in humans and equines residing in Europe. WNV molecular characterization is important for the development of diagnostic assays, as well as for obtaining molecular information, which is necessary for epidemiological investigations of virus transmission in areas at risk. For whole genome sequencing of lineage 2 WNV strains, directly from...
Source: Molecular and Cellular Probes - June 13, 2019 Category: Molecular Biology Source Type: research

Assessment of ctDNA in CSF may be a more rapid means of assessing surgical outcomes than plasma ctDNA in glioblastoma
Publication date: Available online 4 June 2019Source: Molecular and Cellular ProbesAuthor(s): Jue-hui Li, Zhen-qiang He, Fu-hua Lin, Zheng-he Chen, Shi-yu Yang, Hao Duan, Xiao-bing Jiang, Fuad Al-Nahari, Xiang-heng Zhang, Jiang-huang Wang, Guan-hua Zhang, Zhen-feng Zhang, Cong Li, Yong-Gao MouAbstractWe aimed to develop a high-throughput deep DNA sequencing assay of cerebrospinal fluid (CSF) to identify clinically relevant oncogenic mutations that contribute to the development of glioblastoma (GBM) and serve as biomarkers to predict patients' responses to surgery. For this purpose, we recruited five patients diagnosed with...
Source: Molecular and Cellular Probes - June 5, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: June 2019Source: Molecular and Cellular Probes, Volume 45Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 28, 2019 Category: Molecular Biology Source Type: research

Recovery of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box motif (CACATG) deletion within the left terminal region
This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 24, 2019 Category: Molecular Biology Source Type: research

SI: Next-generation sequencing in human molecular genetic diagnostics
Publication date: Available online 15 May 2019Source: Molecular and Cellular ProbesAuthor(s): Sabine Hoffjan (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 15, 2019 Category: Molecular Biology Source Type: research

Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway
ConclusionES suppresses lung cancer progression by down-regulating HGF-Met signaling, revealing the possible mechanism of ES in the process of treating lung cancer patients. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 15, 2019 Category: Molecular Biology Source Type: research

In silico prediction of B-cell epitopes for twenty-five mite allergens: The therapeutic potentials for immunotherapy
Publication date: Available online 14 May 2019Source: Molecular and Cellular ProbesAuthor(s): Narjes Ebrahimi, Navid Nezafat, Hossein Esmaeilzadeh, Younes Ghasemi, Seyed Hesamodin Nabavizadeh, Soheila AlyasinAbstractMite allergens are one of the major allergens; however, their structures and epitopes have not been thoroughly studied. In the present study, we predicted the tertiary structures of several mite allergens and also identified the B-cell epitopes, which can be suggested as potential epitopes for allergen immunotherapy. Twenty-five mite allergens, from six mite allergen groups, were investigated; homology modeling...
Source: Molecular and Cellular Probes - May 15, 2019 Category: Molecular Biology Source Type: research

Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139
Publication date: Available online 10 May 2019Source: Molecular and Cellular ProbesAuthor(s): Hidemasa Izumiya, Masatomo Morita, Eiji Arakawa, Tuan Cuong Ngo, Hoai Thu Nguyen, Dong Tu Nguyen, Makoto OhnishiAbstractA loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 12, 2019 Category: Molecular Biology Source Type: research

Let-7 inhibits the migration and invasion of extravillous trophoblast cell via targeting MDM4
ConclusionsLet-7 silencing promoted proliferation, migration and invasion in EVT cells through the up-regulation of MDM4. Our study provided new insights into the molecular mechanism of PE. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - May 10, 2019 Category: Molecular Biology Source Type: research

NGS-Based genetic testing for heritable cardiovascular diseases. Specific requirements for obtaining informed consent
Publication date: Available online 3 May 2019Source: Molecular and Cellular ProbesAuthor(s): Jörg Schmidtke, Kathrin Wittkowski, Ralf GlaubitzAbstractClinical genetic testing in cardiovascular genetic medicine has undergone rapid changes. Next generation sequencing allows simultaneous testing of all genes associated with any cardiovascular phenotype, and molecular genetic testing for multiple genes has become the standard of practice for cardiovascular medicine. While technical and clinical advantages of multigenic approaches are evident, informed consent procedures have become more complex and challenging to the phys...
Source: Molecular and Cellular Probes - May 4, 2019 Category: Molecular Biology Source Type: research

Association among ACE, ESR1 polymorphisms and preeclampsia in Brazilian pregnant women
ConclusionThis pioneer study involving Brazilian women showed no association among the studied polymorphisms and PE, which suggests that ins/del ACE and SNPs ESR1 do not contribute to this disease occurrence in Brazil. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - April 25, 2019 Category: Molecular Biology Source Type: research

A TaqMan probe-based real-time PCR to differentiate porcine epidemic diarrhea virus virulent strains from attenuated vaccine strains
Publication date: Available online 17 April 2019Source: Molecular and Cellular ProbesAuthor(s): Jing Liu, Li-min Li, Jiu-qaun Han, Tai-ran Sun, Xue Zhao, Rui-tao Xu, Qin-ye SongAbstractPorcine epidemic diarrhea virus (PEDV) is an important pathogen causing severe watery diarrhea, vomiting, dehydration, and death in sucking piglets. Attenuated vaccines have been used widely in sows in order to protect piglets through passive lactogenic immunity. Rapid and sensitive detection methods for differentiating attenuated vaccine strains from virulent ones are essential and practical in PEDV prevention and control. Based on the dele...
Source: Molecular and Cellular Probes - April 18, 2019 Category: Molecular Biology Source Type: research

An exonuclease-assisted fluorescence sensor for assaying alkaline phosphatase based on SYBR Green I
In this report, we propose a fast, reliable and convenient approach to determine the alkaline phosphatase (ALP) activity based on a label-free fluorescence strategy. Upon catalysis of ALP, dephosphorylated dsDNA hampers the λ exonuclease (λexo) cleavage, shows high affinity to SYBR Green I (SG I), resulting in a strong fluorescence emission peak at 520 nm. In the absence of ALP, the dsDNA with 5′-phosphoryl-termini could be employed as a substrate of λexo. After cleavage, a weak fluorescence emission peaks at 520 nm could be observed. The assay was both selective and sensitive, and the detect...
Source: Molecular and Cellular Probes - April 13, 2019 Category: Molecular Biology Source Type: research

Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3
Publication date: Available online 10 April 2019Source: Molecular and Cellular ProbesAuthor(s): Yu Zhao, Hao-ying Han, Lin Fan, Run-Bo Tian, Jian-Tao Cui, Jin-Yi Li, Hong-Ying Chen, Ming-Fan Yang, Lan-Lan ZhengAbstractPorcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differentially detect PCV2 and PCV3. The assay specifically detected PCV2 and PCV3, with no fluores...
Source: Molecular and Cellular Probes - April 11, 2019 Category: Molecular Biology Source Type: research

Identification of FimH derivatives as adjuvant vaccinated with PAc that enhance protection against Streptcoccus mutans colonization
In this study, adjuvant potential of FimH variants was evaluated by investigating their ability to trigger innate immune response to DC2.4 and adaptive immunity to improve immunological characteristics. The data revealed that purified A106 and T158P up-regulated the expression of co-stimulatory molecules critically involved in DC2.4 activation by interaction with TLR4, whereas A25P and G73R did not induce the phenotypic maturation of DC2.4. Besides, the culture of DC2.4 with A106 and T158P enhanced the release of cytokines and protein phagocytosis. When formulated with PAc, T158P elicited more robust PAc-specific IgG and I...
Source: Molecular and Cellular Probes - March 31, 2019 Category: Molecular Biology Source Type: research

Fabry disease: Detection of Alu-mediated exon duplication by NGS
Publication date: Available online 29 March 2019Source: Molecular and Cellular ProbesAuthor(s): Martin Farr, Susana Ferreira, Arwa Al-Dilaimi, Sonja Bögeholz, Alexander Goesmann, Jörn Kalinowski, Cornelius Knabbe, Lothar Faber, João Paulo Oliveira, Volker RudolphAbstractMonogenetic diseases can be analyzed routinely by targeted DNA sequencing. If causative variants are not found, complementary methods like RNA sequencing or analysis of copy number variations by multiplex ligation-dependent probe amplification have to be considered. In the latter, especially exonic duplications or deletions can be detected,...
Source: Molecular and Cellular Probes - March 30, 2019 Category: Molecular Biology Source Type: research

Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay
In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was developed to rapid detect A. pleuropneumoniae. Real-time RPA was performed successfully in Genie III at the constant temperature of 39 °C for 20 min. The developed assay was highly specific for A. pleuropneumoniae, and the sensitivity at 95% probability was 536 fg of A. pleuropneumoniae genomic DNA. The real-time RPA for A. pleuropneumoniae was further evaluated on the 112 clinical swine lung and tonsil samples, and 25 (22.3%), 27 (24.1%), and 12 (10.7%) samples were positive for A. pleuropneumoniae ...
Source: Molecular and Cellular Probes - March 29, 2019 Category: Molecular Biology Source Type: research

Corrigendum to “Enhanced multiplex PCR assay for the typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus”
Publication date: Available online 28 March 2019Source: Molecular and Cellular ProbesAuthor(s): Kunyan Zhang, Jo-Ann McClure, John M. Conly (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 28, 2019 Category: Molecular Biology Source Type: research

Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of human respiratory syncytial virus
Publication date: Available online 25 March 2019Source: Molecular and Cellular ProbesAuthor(s): Yun Xi, Chang-Zhi Xu, Zhi-Zhi Xie, Dong-Lin Zhu, Jie-Ming Dong, Gang XiaoAbstractRespiratory syncytial virus (RSV) is one of the most important causative agents that causing respiratory tract infection in children and associated with high morbidity and mortality. A diagnostic method would be a robust tool for identification of RSV infection, especially in the resource-limited settings. Recombinase polymerase amplification (RPA) is a novel isothermal amplification technique which has been widely employed to detect human/animal pa...
Source: Molecular and Cellular Probes - March 26, 2019 Category: Molecular Biology Source Type: research

Which genes to assess in the NGS diagnostics of intellectual disability? The case for a consensus database-driven and expert-curated approach
Publication date: Available online 23 March 2019Source: Molecular and Cellular ProbesAuthor(s): Florian Erger, Christian P. Schaaf, Christian NetzerAbstractWhen deciding on which genes to assess in larger Next-Generation Sequencing (NGS) datasets for the molecular genetic diagnosis of intellectual disability (ID), geneticists today have a variety of gene-phenotype databases and expert-curated gene lists available. To quantify their respective completeness, we compare an ID gene selection auto-generated from the Human Phenotype Ontology gene-phenotype association database and expert-curated ID gene lists from three reputabl...
Source: Molecular and Cellular Probes - March 24, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2019Source: Molecular and Cellular Probes, Volume 44Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 24, 2019 Category: Molecular Biology Source Type: research

Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA
In conclusion, IsoPCR is a highly sensitive and specific diagnostic tool and it is suitable for the detection of low copy number of viral DNA in clinical specimens, providing critical information to healthcare providers. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 21, 2019 Category: Molecular Biology Source Type: research

Use of whole exome sequencing in the NICU: Case of an extremely low birth weight infant with syndromic features
In conclusion, our case illustrated that CS should be considered in ELBW infants showing a life-threatening combination of complex cardiac arrhythmia and hypokinesia. If a syndromic disorder is suspected in the neonatal intensive care unit (NICU) setting, WES is a useful, non-invasive diagnostic tool in critically ill ELBW infants. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - March 15, 2019 Category: Molecular Biology Source Type: research

Probe-specific loop-mediated isothermal amplification magnetogenosensor assay for rapid and specific detection of pathogenic Leptospira
This study highlighted the performance of the developed integrated loop-mediated isothermal amplification (LAMP) coupled with a colorimetric DNA-based magnetogenosensor. The biosensor operates through a DNA hybridization system in which a specific designed probe captures the target LAMP amplicons. We demonstrated the magnetogenosensor assay by detecting pathogenic Leptospira, which causes leptospirosis. The color change of the assay from brown to blue indicated a positive result, whereas a negative result was indicated by the assay maintaining its brown color. The DNA biosensor was able to detect DNA at a concentration as ...
Source: Molecular and Cellular Probes - March 13, 2019 Category: Molecular Biology Source Type: research

CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin
Publication date: Available online 22 February 2019Source: Molecular and Cellular ProbesAuthor(s): Keyi Li, Zhen Meng, Licheng Jiang, Chunpeng Xia, Kai Xu, Daoying Yuan, Haiying Chen, Bin Zhang, Shuwei LiuAbstractCDKL1 is a cyclin-dependent kinase-like kinase that is highly expressed in diverse types of cancer cells. However, the role of CDKL1 in the chemoresistance of oral squamous cell carcinoma (OSCC) remains largely undefined. Here, we explored the role of CDKL1 in the chemoresistance of the human OSCC cell line CAL27 to hydroxycamptothecin (HCPT). Real-time quantitative polymerase chain reaction and western blotting r...
Source: Molecular and Cellular Probes - February 23, 2019 Category: Molecular Biology Source Type: research

EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2
In conclusion, we report that a highly sensitive, specific, and cost-effective EvaGreen-based real-time PCR assay is successful for the rapid detection of ENTV-2. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - February 14, 2019 Category: Molecular Biology Source Type: research

Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3
Publication date: Available online 5 February 2019Source: Molecular and Cellular ProbesAuthor(s): Hao-ying Han, Hui-hua Zheng, Yu Zhao, Run-bo Tian, Peng-Li Xu, Hua-lin Hou, Hong-Ying Chen, Ming-Fan YangAbstractThe development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for other non-targeted pig pathogens. The assay showed a good linear relationship, and the limits of detect...
Source: Molecular and Cellular Probes - February 6, 2019 Category: Molecular Biology Source Type: research

MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1
ConclusionmiR-486-5p may inhibit the proliferation of leukemia cells and induce apoptosis through targeting FOXO1. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - February 5, 2019 Category: Molecular Biology Source Type: research

MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL
In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. When miR-224 was either overexpressed or silenced, qRT-PCR showed a negative regulatory effect on LPL. mRNA expression levels of the f...
Source: Molecular and Cellular Probes - January 28, 2019 Category: Molecular Biology Source Type: research

Downregulated low-density lipoprotein receptor-related protein 6 induces the maldevelopment of extravillous trophoblast via Wnt/β-catenin signaling pathway
Publication date: Available online 24 January 2019Source: Molecular and Cellular ProbesAuthor(s): Xiaoyan Yu, Yan Zhang, Ping Yang, Xueli Gao, Yuping WangAbstractPreeclampsia (PE), a special type of hypertensive disorder complicating pregnancy (HDCP), is highly associated with the migratory and invasive capacity of extravillous trophoblasts (EVTs). Here, we aimed to study the functions of low-density lipoprotein receptor-related protein 6 (LRP6) in PE pathogenesis. A comparative analysis of cellular gene expressions between placenta tissues collected from PE patients and normal pregnant women showed that the expressions of...
Source: Molecular and Cellular Probes - January 24, 2019 Category: Molecular Biology Source Type: research

HADHA and HADHB gene associated phenotypes - Identification of rare variants in a patient cohort by Next Generation Sequencing
Publication date: Available online 22 January 2019Source: Molecular and Cellular ProbesAuthor(s): Isabel Diebold, Ulrike Schön, Rita Horvath, Oliver Schwartz, Elke Holinski-Feder, Heike Kölbel, Angela AbichtAbstractThe heterooctameric mitochondrial trifunctional protein (MTP), composed of four α- and β-subunits harbours three enzymes that each perform a different function in mitochondrial fatty acid β-oxidation. Pathogenic variants in the MTP genes (HADHA and HADHB) cause MTP deficiency, a rare autosomal recessive metabolic disorder characterized by phenotypic heterogeneity ranging from severe, ea...
Source: Molecular and Cellular Probes - January 23, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: February 2019Source: Molecular and Cellular Probes, Volume 43Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 20, 2019 Category: Molecular Biology Source Type: research

Novel dual-color drug screening model for GLUT4 translocation in adipocytes
In conclusion, we developed an easy-to-apply dual color GLUT4 probe to monitor GLUT4 translocation in insulin-responsive cells, which could be alternatively employed to high-throughput screen compounds regulating GLUT4 translocation and glucose uptake, even to dissect GLTU4 approaching, docking and fusion with the plasma membrane (PM), and to reveal relevant molecular mechanisms involved in these steps as expected. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - January 12, 2019 Category: Molecular Biology Source Type: research

A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi
Publication date: Available online 3 January 2019Source: Molecular and Cellular ProbesAuthor(s): Jianhu Pang, Qiong Wang, Yuejun Fei, Peng Zhu, Longliang Qiao, Hailong Huang, Chenyang Dang, Weifang GaoAbstractVibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast and accurate method to detect V. harveyi is required. Here, we performed recombinase polymerase amplification (RPA) using novel primers specifically designe...
Source: Molecular and Cellular Probes - January 3, 2019 Category: Molecular Biology Source Type: research

Next generation sequencing and imprinting disorders: Current applications and future perspectives: Lessons from Silver-Russell syndrome
Publication date: Available online 2 January 2019Source: Molecular and Cellular ProbesAuthor(s): Lea Neuheuser, Robert Meyer, Matthias Begemann, Miriam Elbracht, Thomas EggermannAbstractImprinting Disorders are a group of rare diseases with overlapping phenotypes which are associated with similar molecular changes and affect imprinted chromosomal regions. Clinical features mainly occur prenatally or in childhood, but have a severe lifelong impact on health. Due to their clinical and molecular heterogeneity, the diagnosis of imprinting disorders is often challenging and requires testing of a broad spectrum of genomic varian...
Source: Molecular and Cellular Probes - January 2, 2019 Category: Molecular Biology Source Type: research

Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood
Publication date: Available online 22 December 2018Source: Molecular and Cellular ProbesAuthor(s): Mark Jain, Alexander Vladimirovich Balatsky, Daria Borisovna Revina, Larisa Mikhailovna SamokhodskayaAbstractBackgroundBlood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isolation of cffDNA, which is a crucial step for success of subsequent analysis.AimTo compare DSPVK and CNAK in term...
Source: Molecular and Cellular Probes - December 23, 2018 Category: Molecular Biology Source Type: research

Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification
Publication date: Available online 22 December 2018Source: Molecular and Cellular ProbesAuthor(s): Rong Zeng, Jinyan Luo, Shigang Gao, Lihui Xu, Zhiwei Song, Fuming DaiAbstractReverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 22, 2018 Category: Molecular Biology Source Type: research

Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB
Publication date: Available online 18 December 2018Source: Molecular and Cellular ProbesAuthor(s): Roberto Cruz-Flores, Hung Nam Mai, Arun K. DharAbstractAcute hepatopancreatic necrosis disease (AHPND), also known as Early mortality syndrome (EMS), is a recently emerged lethal disease that has caused major economic losses in shrimp aquaculture. The etiologic agents are Vibrio spp. that carry Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB. A multiplex SYBR Green real-time PCR was developed that detects pirA, pirB, and two internal control genes, the shrimp 18S rRNA and the bacterial 16S rRNA genes in a single r...
Source: Molecular and Cellular Probes - December 19, 2018 Category: Molecular Biology Source Type: research

A novel fluorometric method for inorganic pyrophosphatase detection based on G-quadruplex-thioflavin T
Publication date: Available online 17 December 2018Source: Molecular and Cellular ProbesAuthor(s): Han Zhao, Changbei Ma, Mingjian ChenAbstractIn this paper, we propose a fluorometric approach for the highly sensitive detection of inorganic pyrophosphatase (PPase) based on G-quadruplex-thioflavin T (ThT). In the absence of PPase, Cu2+ can coordinate with pyrophosphate (PPi) to generate a Cu2+/PPi complex. Then the G-rich sequence folds into the G-quadruplex structure, which can combine with ThT to generate a remarkable fluorescent signal. In the presence of PPase, the coordinated compound can be destroyed by the PPase cata...
Source: Molecular and Cellular Probes - December 18, 2018 Category: Molecular Biology Source Type: research