The contribution of GTF2I haploinsufficiency to Williams syndrome
Publication date: Available online 3 January 2018Source: Molecular and Cellular ProbesAuthor(s): Thanathom Chailangkarn, Chalongrat Noree, Alysson R. MuotriAbstractWilliams syndrome (WS) is a neurodevelopmental disorder involving hemideletion of as many as 26–28 genes, resulting in a constellation of unique physical, cognitive and behavior phenotypes. The haploinsufficiency effect of each gene has been studied and correlated with phenotype(s) using several models including WS subjects, animal models, and peripheral cell lines. However, links for most of the genes to WS phenotypes remains unclear. Among those genes, g...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Modeling rare diseases with induced pluripotent stem cell technology
Publication date: Available online 5 January 2018Source: Molecular and Cellular ProbesAuthor(s): Ruthellen H. Anderson, Kevin R. FrancisAbstractRare diseases, in totality, affect a significant proportion of the population and represent an unmet medical need facing the scientific community. However, the treatment of individuals affected by rare diseases is hampered by poorly understood mechanisms preventing the development of viable therapeutics. The discovery and application of cellular reprogramming to create novel induced pluripotent stem cell models of rare diseases has revolutionized the rare disease community. Through...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Peroxisomal disorders: Improved laboratory diagnosis, new defects and the complicated route to treatment
Publication date: Available online 10 February 2018Source: Molecular and Cellular ProbesAuthor(s): Ronald J.A. WandersAbstractPeroxisomes catalyze a number of essential metabolic functions of which fatty acid alpha- and beta-oxidation, ether phospholipid biosynthesis, glyoxylate detoxification and bile acid synthesis are the most important. The key role of peroxisomes in humans is exemplified by the existence of a group of peroxisomal disorders, caused by mutations in > 30 different genes which code for proteins with a role in either peroxisome biogenesis or one of the metabolic pathways in peroxisomes. Technologica...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Colorimetric detection of microRNA based on DNAzyme and nuclease-assisted catalytic hairpin assembly signal amplification
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju, Jiayu WanAbstractAccurate and quantitative analysis of microRNA (miRNA) expression is critical for the diagnostics and theranostics of a disease. Herein, a proof-of-concept of a colorimetric horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) biosensor for miRNA assay based on nuclease-assisted catalytic hairpin assembly (CHA) signal amplification was demonstrated. Duplex-specific nuclease (DSN) was employed to cleave the single-stranded DNA (ssDNA) chimeric probe (CP) ...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5
In this study, we focused on the roles of OGG1 in histone modification. First, to study the effects of OGG1 on histone modification, the protein levels of symmetric dimethylation of histone H4 arginine-3 (H4R3me2s) were determined by western blot analysis following the knockdown or overexpression of OGG1. Second, the molecular mechanisms by which OGG1 regulates H4R3me2s were assessed by co-immunoprecipitation (CO-IP) assays in mouse embryonic fibroblast (MEF) wild-type (WT) and Ogg−/− cells. Finally, to verify the regulation of H4R3me2s by OGG1 on specific genes, chromatin immunoprecipitation (CHIP) was perform...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Structure-activity modelling of essential oils, their components, and key molecular parameters and descriptors
This study aimed to elucidate SARs of essential oil components from experimental and literature sources. Minimum Inhibitory Concentrations (MICs) of essential oil components were determined against Escherichia coli and Staphylococcus aureus using a microdilution method and then compared to those in published in literature. Of 12 essential oil components tested, carvacrol and cuminaldehyde were most potent with MICs of 1.98 and 2.10 mM, respectively. The activity of 21 compounds obtained from the literature, MICs ranged from 0.004 mM for limonene to 36.18 mM for α-terpineol. A 3D qualitative SAR model was genera...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus
In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. RPA primers and LF probes were designed by targeting the specific G gene, and the amplification product can be visualized on a simple lateral flow dipstick with the naked eyes. The amplification reaction was performed at 38 °C for 20 min and LFD incubation time within 5 min. The detection limit of this assay was 8 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses such as bovine viral diarrhea virus, infectious bovine ...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cel...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of a SYBR Green I real-time PCR for detection and quantitation of orthopoxvirus by using Ectromelia virus
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Wenyu Cheng, Xiaobing He, Huaijie Jia, Guohua Chen, Cong Wang, Jun Zhang, Zhizhong JingAbstractEctromelia virus (ECTV) is the causative agent of mousepox, which has devastating effects in laboratory-mouse colonies and causes economic loss in biomedical research. More importantly, ECTV has been extensively used as an excellent model for studies of the pathogenesis and immunobiology of human smallpox. A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by u...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of HyBeacon® probes for specific mRNA detection using body fluids as a model system
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Beccy Stafford-Allen, Nick Dawnay, Erin K. Hanson, Glyn Ball, Ambika Gupta, Stephen Blackman, David J. French, Nicola Duxbury, Jack Ballantyne, Simon WellsAbstractHyBeacons are linear oligonucleotides which incorporate fluorescent dyes covalently linked to internal nucleotides. They have previously been used with PCR and isothermal amplification to interrogate SNPs and STRs in fields as diverse as clinical diagnostics, food authentication, and forensic DNA profiling. This work explores their use for the identification of expressed gene s...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

WITHDRAWN: Designing and computational validation of microarray probe sets for the diagnosis of viruses of non-human vertebrates
In this study, a microarray probe dataset was strategized exclusively targeting the diagnosis of viruses infecting non-human vertebrates. The dataset contains 21,234 species-specific probes from 766 pathogenic virus species and subtypes and 3998 genus level conserved probes from 146 virus genera. The probes were designed from coding regions of virus genomes and selected after rigorous screening for species specificities, minimal cross-reactivity and thermodynamic properties. The probe sets were computationally compared with the probes of earlier published pan-viral arrays; GreeneChipPm, ViroChip and Microbial Detection Arr...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development and validation of pan-Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens
ConclusionThe findings suggest that the developed Taqman qPCR assay is sensitive, specific and has potential to be applied in a larger subsequent study. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Yaru Sun, Yuening Cheng, Peng Lin, Li Yi, Mingwei Tong, Zhigang Cao, Gaili Wang, Shuang Li, Shipeng Cheng, Wanzhe Yuan, Jianke WangAbstractCanine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), w...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method
Publication date: Available online 22 May 2018Source: Molecular and Cellular ProbesAuthor(s): Zhiqing Huang, Jianwei Zheng, Chunmei Shi, Qiang ChenAbstractCoupling propidium monoazide (PMA) with quantitative PCR (PMA-qPCR) has been successfully applied to specific detection and quantification of viable cells in various samples. The optimal PMA treatment condition is usually determined through qPCR. However, it is a tedious, time consuming and costly process including DNA extraction and qPCR. To overcome this problem, a flow cytometry-based (FCM-based) method was first proposed in this study to replace qPCR for screening of...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

The development and application of a duplex reverse transcription loop-mediated isothermal amplification assay combined with a lateral flow dipstick method for Macrobrachium rosenbergii nodavirus and extra small virus isolated in China
In conclusion, a convenient, sensitive and selective practical molecular diagnostic method was developed with the potential for diagnosis and prevention of WTD. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Identification of bta-miR-15a∼16a cluster expression, localization and regulated target in Holsteins
In this study, we investigated the expression pattern and tissue localization of bta-miR-15a and bta-miR-16a. The expression levels of bta-miR-15a and bta-miR-16a were significantly upregulated in mammary tissues and blood neutrophils of mastitis-infected cows, compared with those of healthy cows (P 
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

A novel HRM assay for differentiating classical strains and highly pathogenic strains of type 2 porcine reproductive and respiratory syndrome virus
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Junying Sun, Gali Bingga, Zhicheng Liu, Chunhong Zhang, Haiyan Shen, Pengju Guo, Jianfeng ZhangAbstractDifferentiation of classical strains and highly pathogenic strains of porcine reproductive and respiratory syndrome virus is crucial for effective vaccination programs and epidemiological studies. We used nested PCR and high resolution melting curve analysis with unlabeled probe to distinguish between the classical and the highly pathogenic strains of this virus. Two sets of primers and a 20 bp unlabeled probe were designed from the NSP3...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Changes in gene expression in lungs of mice exposed to traffic-related air pollution
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Jie Yang, Yi Chen, Zhi Yu, Hui Ding, Zhongfu MaAbstractLong-term exposure to traffic-related pollutants can lead to a variety of respiratory diseases, including inflammation, asthma, and lung cancer; however, the underlying biological mechanisms are not fully understood. We focused on the effects of exposure to different air pollutants on the expression of genes associated with inflammatory immune responses, allergic reactions and asthma, and lung cancer. In order to understand the cellular responses induced by exposure to different traff...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Libing Liu, Jianchang Wang, Yunyun Geng, Jinfeng Wang, Ruiwen Li, Ruihan Shi, Wanzhe YuanAbstractA visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could de...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

CRISPR-Cas9; an efficient tool for precise plant genome editing
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Waqar IslamAbstractEfficient plant genome editing is dependent upon induction of double stranded DNA breaks (DSBs) through site specified nucleases. These DSBs initiate the process of DNA repair which can either base upon homologous recombination (HR) or non-homologous end jointing (NHEJ). Recently, CRISPR-Cas9 mechanism got highlighted as revolutionizing genetic tool due to its simpler frame work along with the broad range of adaptability and applications. So, in this review, I have tried to sum up the application of this biotechnologica...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Rapid detection of goose hemorrhagic polyomavirus using TaqMan quantitative real-time PCR
In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses. The standard curve had a linear correlation of 0.997 and efficiency of 99% between the cycle threshold value and the logarithm of the plasmids copy number. The possible lowest detectable concentration was 35.4 copies/μl; 100 times more sensitive than conventional PCR (detection limit, 3.54 × 103 copies/μl). Domestic Jinyun Sheldrakes ducks and their embryonated eggs were found positive of GHPV infection which provides eviden...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

The establishment of methods for free PAR generation and PAR reader detection
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Yueshuang Ke, Ke Wang, Hui Xu, Chenxin Wang, Jing Zhang, Xianlu Zeng, Ruoxi Wang, Istvan Boldogh, Xueqing BaAbstractPoly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor that catalyzes the poly (ADP-ribose) (PAR) onto a variety of target proteins, such as histones, DSB repair factors and PARP1 itself under consumption of NAD+. Besides, PARP1 can affect a variety of proteins in noncovalent modification manner to carry out specific cellular functions. Here, we established a method to generate non-radiolabeled free PAR by PARG modera...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Chunhe Wan, Cuiteng Chen, Longfei Cheng, Guanghua Fu, Shaohua Shi, Qiuling Fu, Rongchang Liu, Hongmei Chen, Yu HuangAbstractPigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a TaqMan-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/μl. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the t...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Comparative application of IS711-based polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) for canine brucellosis diagnosis
This study evaluated the potential of molecular tools based on PCR and LAMP using primers targeting the insertion sequence IS711 for Brucella detection in three groups of dogs (infected, non-infected and suspected of brucellosis), which were determined according to the results of blood culturing and clinical examination. The performance of the three diagnostic tests was also determined using McNemar test and Kappa coefficient. The proportion of positive samples detected by blood culturing, PCR and LAMP was respectively 31.57% (18/57), 33.34% (19/57), and 14.03% (8/57). The agreement between blood culturing and PCR was almo...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

EvaGreen-based real-time PCR assay for sensitive detection of salmonid alphavirus
In this study, we developed a novel, sensitive, low-cost detection method, EvaGreen-based real-time PCR assay for the detection of SAV. This assay exhibited high specificity for SAV1, SAV2, and SAV5 and was able to detect SAV at concentrations as low as 1.5 × 101 copies, making them more sensitive than the approved conventional RT-PCR method (detection limit, 1.5 × 106 copies). Assessment of infected fish samples showed that the sensitivity of EvaGreen-based assay was higher than previously developed SYBR Green assay (227 assay). Thus, we report that the EvaGreen real-time PCR assays is an economical al...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

An in vitro and in silico identification of antibiofilm small molecules from seawater metaclone SWMC166 against Vibrio cholerae O1
This study aimed to determine the antibiofilm activity of seawater microbes against Vibrio cholerae (VCO1) through functional metagenomics approach. A metagenomic library was constructed from Palk Bay seawater and the library was screened to identify the biofilm inhibitory metaclone. Metaclone SWMC166 (harbouring ∼30 kb metagenomic insert) was found to exhibit antibiofilm activity against VCO1. The biofilm inhibitory potential of partially purified ethyl acetate extract of SWMC166 (EA166) was further evaluated through microscopic studies and biochemical assays. Further, EA166 treated VCO1 divulged up-regulation of ge...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples
In conclusion, this method could be taken into account as an alternative technical approach to detect KIPyV and/or WUPyV in respiratory samples for epidemiological and diagnostic analyses. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Design and evaluation of nested PCR primers for specific detection of genogroup I noroviruses in oysters
Publication date: Available online 11 June 2018Source: Molecular and Cellular ProbesAuthor(s): Ping Guo, Yongxin Yu, Yingjie Pan, Shuling Yan, Yongjie WangAbstractA pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

MiR-1297 suppresses pancreatic cancer cell proliferation and metastasis by targeting MTDH
In conclusion, our results suggest that miR-1297 inhibits the growth and metastasis of pancreatic adenocarcinoma by downregulating MTDH expression, and the miR-1297/MTDH pathway is a potential target for treating pancreatic adenocarcinoma. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Differential expressions of estrogen and progesterone receptors in endometria and cyst walls of ovarian endometrioma from women with endometriosis and their responses to depo-medroxyprogesterone acetate treatment
Publication date: Available online 3 July 2018Source: Molecular and Cellular ProbesAuthor(s): Morakot Sroyraya, Sineenart Songkoomkrong, Narin Changklungmoa, Jaruwan Poljaroen, Sawaek Weerakiet, Areepan Sophonsritsuk, Anna Wongkularb, Srithean Lertvikool, Yada Tingthanatikul, Prasert SobhonAbstractBackgroundDepo-medroxyprogesterone acetate (DMPA) is an injectable progestin contraceptive that provides a highly effective reduction of pelvic pain in women with endometriosis. Despite its wide use to treat pain associated with endometriosis, its precise mechanisms of action remain unclear. The aims of this study were to investi...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Gold nanoparticle biosensors, a novel application in gene transformation and expression
Publication date: Available online 7 July 2018Source: Molecular and Cellular ProbesAuthor(s): Yaser Ghazi, Fatemeh Haddadi, Hossein KamaladiniAbstractThe conventional techniques of PCR, Southern blot, Northern blot, in situ hybridization, and RNase protection assay have long been used to investigate transformation and expression of genes, but most of them are time-consuming and have relatively low sensitivity. In recent years, applying biosensors for molecular identification of biomolecules has been expanding significantly. Hence in this study, Zabol mildew melon was used as a model plant to introduce new DNA and RNA-based...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

The contribution of GTF2I haploinsufficiency to Williams syndrome
Publication date: Available online 3 January 2018Source: Molecular and Cellular ProbesAuthor(s): Thanathom Chailangkarn, Chalongrat Noree, Alysson R. MuotriAbstractWilliams syndrome (WS) is a neurodevelopmental disorder involving hemideletion of as many as 26–28 genes, resulting in a constellation of unique physical, cognitive and behavior phenotypes. The haploinsufficiency effect of each gene has been studied and correlated with phenotype(s) using several models including WS subjects, animal models, and peripheral cell lines. However, links for most of the genes to WS phenotypes remains unclear. Among those genes, g...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Modeling rare diseases with induced pluripotent stem cell technology
Publication date: Available online 5 January 2018Source: Molecular and Cellular ProbesAuthor(s): Ruthellen H. Anderson, Kevin R. FrancisAbstractRare diseases, in totality, affect a significant proportion of the population and represent an unmet medical need facing the scientific community. However, the treatment of individuals affected by rare diseases is hampered by poorly understood mechanisms preventing the development of viable therapeutics. The discovery and application of cellular reprogramming to create novel induced pluripotent stem cell models of rare diseases has revolutionized the rare disease community. Through...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Peroxisomal disorders: Improved laboratory diagnosis, new defects and the complicated route to treatment
Publication date: Available online 10 February 2018Source: Molecular and Cellular ProbesAuthor(s): Ronald J.A. WandersAbstractPeroxisomes catalyze a number of essential metabolic functions of which fatty acid alpha- and beta-oxidation, ether phospholipid biosynthesis, glyoxylate detoxification and bile acid synthesis are the most important. The key role of peroxisomes in humans is exemplified by the existence of a group of peroxisomal disorders, caused by mutations in > 30 different genes which code for proteins with a role in either peroxisome biogenesis or one of the metabolic pathways in peroxisomes. Technologica...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5
In this study, we focused on the roles of OGG1 in histone modification. First, to study the effects of OGG1 on histone modification, the protein levels of symmetric dimethylation of histone H4 arginine-3 (H4R3me2s) were determined by western blot analysis following the knockdown or overexpression of OGG1. Second, the molecular mechanisms by which OGG1 regulates H4R3me2s were assessed by co-immunoprecipitation (CO-IP) assays in mouse embryonic fibroblast (MEF) wild-type (WT) and Ogg−/− cells. Finally, to verify the regulation of H4R3me2s by OGG1 on specific genes, chromatin immunoprecipitation (CHIP) was perform...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Structure-activity modelling of essential oils, their components, and key molecular parameters and descriptors
This study aimed to elucidate SARs of essential oil components from experimental and literature sources. Minimum Inhibitory Concentrations (MICs) of essential oil components were determined against Escherichia coli and Staphylococcus aureus using a microdilution method and then compared to those in published in literature. Of 12 essential oil components tested, carvacrol and cuminaldehyde were most potent with MICs of 1.98 and 2.10 mM, respectively. The activity of 21 compounds obtained from the literature, MICs ranged from 0.004 mM for limonene to 36.18 mM for α-terpineol. A 3D qualitative SAR model was genera...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus
In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. RPA primers and LF probes were designed by targeting the specific G gene, and the amplification product can be visualized on a simple lateral flow dipstick with the naked eyes. The amplification reaction was performed at 38 °C for 20 min and LFD incubation time within 5 min. The detection limit of this assay was 8 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses such as bovine viral diarrhea virus, infectious bovine ...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cel...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Development of a SYBR Green I real-time PCR for detection and quantitation of orthopoxvirus by using Ectromelia virus
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Wenyu Cheng, Xiaobing He, Huaijie Jia, Guohua Chen, Cong Wang, Jun Zhang, Zhizhong JingAbstractEctromelia virus (ECTV) is the causative agent of mousepox, which has devastating effects in laboratory-mouse colonies and causes economic loss in biomedical research. More importantly, ECTV has been extensively used as an excellent model for studies of the pathogenesis and immunobiology of human smallpox. A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by u...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Development of HyBeacon® probes for specific mRNA detection using body fluids as a model system
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Beccy Stafford-Allen, Nick Dawnay, Erin K. Hanson, Glyn Ball, Ambika Gupta, Stephen Blackman, David J. French, Nicola Duxbury, Jack Ballantyne, Simon WellsAbstractHyBeacons are linear oligonucleotides which incorporate fluorescent dyes covalently linked to internal nucleotides. They have previously been used with PCR and isothermal amplification to interrogate SNPs and STRs in fields as diverse as clinical diagnostics, food authentication, and forensic DNA profiling. This work explores their use for the identification of expressed gene s...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

WITHDRAWN: Designing and computational validation of microarray probe sets for the diagnosis of viruses of non-human vertebrates
In this study, a microarray probe dataset was strategized exclusively targeting the diagnosis of viruses infecting non-human vertebrates. The dataset contains 21,234 species-specific probes from 766 pathogenic virus species and subtypes and 3998 genus level conserved probes from 146 virus genera. The probes were designed from coding regions of virus genomes and selected after rigorous screening for species specificities, minimal cross-reactivity and thermodynamic properties. The probe sets were computationally compared with the probes of earlier published pan-viral arrays; GreeneChipPm, ViroChip and Microbial Detection Arr...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Development and validation of pan-Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens
ConclusionThe findings suggest that the developed Taqman qPCR assay is sensitive, specific and has potential to be applied in a larger subsequent study. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Yaru Sun, Yuening Cheng, Peng Lin, Li Yi, Mingwei Tong, Zhigang Cao, Gaili Wang, Shuang Li, Shipeng Cheng, Wanzhe Yuan, Jianke WangAbstractCanine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), w...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Colorimetric detection of microRNA based on DNAzyme and nuclease-assisted catalytic hairpin assembly signal amplification
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju, Jiayu WanAbstractAccurate and quantitative analysis of microRNA (miRNA) expression is critical for the diagnostics and theranostics of a disease. Herein, a proof-of-concept of a colorimetric horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) biosensor for miRNA assay based on nuclease-assisted catalytic hairpin assembly (CHA) signal amplification was demonstrated. Duplex-specific nuclease (DSN) was employed to cleave the single-stranded DNA (ssDNA) chimeric probe (CP) ...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method
Publication date: Available online 22 May 2018Source: Molecular and Cellular ProbesAuthor(s): Zhiqing Huang, Jianwei Zheng, Chunmei Shi, Qiang ChenAbstractCoupling propidium monoazide (PMA) with quantitative PCR (PMA-qPCR) has been successfully applied to specific detection and quantification of viable cells in various samples. The optimal PMA treatment condition is usually determined through qPCR. However, it is a tedious, time consuming and costly process including DNA extraction and qPCR. To overcome this problem, a flow cytometry-based (FCM-based) method was first proposed in this study to replace qPCR for screening of...
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research

The development and application of a duplex reverse transcription loop-mediated isothermal amplification assay combined with a lateral flow dipstick method for Macrobrachium rosenbergii nodavirus and extra small virus isolated in China
In conclusion, a convenient, sensitive and selective practical molecular diagnostic method was developed with the potential for diagnosis and prevention of WTD. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 5, 2018 Category: Molecular Biology Source Type: research