Application of random amplified polymorphism DNA and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism to predict major Streptococcus suis clonal complexes isolated from humans and pigs
This study revealed that RAPD had a greater discriminatory power than PCR-RFLP. This assay will be useful for screening or predicting major CCs relevant to human and pig S. suis clinical isolates and for low-cost screening of large numbers of isolates with rapid analytical capacity and could be utilized in most laboratories. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - December 6, 2018 Category: Molecular Biology Source Type: research

Ginsenoside Rb1 and mitochondria: A short review of the literature
Publication date: Available online 4 December 2018Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Weijie Xie, Yifan Sun, Ziru Dai, Guang Li, Guibo Sun, Xiaobo SunAbstractMitochondria play a central role in various critical cellular processes, including energy synthesis, energy supply and apoptosis. Panax notoginseng, a commonly used traditional Chinese medicine, has various pharmacological effects on the human body. Ginsenosides are representative bioactive components of P. notoginseng. Recently, more attention has focused on ginsenoside Rb1 as an antioxidative and anti-inflammatory agent that can protect the ne...
Source: Molecular and Cellular Probes - December 5, 2018 Category: Molecular Biology Source Type: research

Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population
Publication date: Available online 29 November 2018Source: Molecular and Cellular ProbesAuthor(s): Malik Siddique Mahmood, Muhammad Asad Ullah, Hina Batool, Sana Batool, Naeem Mehmood AshrafAbstractGonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Although extensive researches are ongoing to contro...
Source: Molecular and Cellular Probes - November 29, 2018 Category: Molecular Biology Source Type: research

Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids
Publication date: Available online 22 November 2018Source: Molecular and Cellular ProbesAuthor(s): Sadaomi Sugimoto, Hiroshi Maeda, Mizuki Kitamatsu, Ikuo Nishikawa, Muneyasu ShidaAbstractPeptide nucleic acids (PNA) are DNA/RNA analogs in which the sugar-phosphate backbone is replaced by N-2-aminoethylglycine. PNA are widely used for experimental antisense therapy due to their strong affinity to mRNA. By targeting specific genes, protein synthesis and the growth of bacteria or cancer cells can be inhibited by PNA. Here, we report the design and evaluation of antisense PNA for selective growth inhibition of Porphyromonas gi...
Source: Molecular and Cellular Probes - November 22, 2018 Category: Molecular Biology Source Type: research

Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3
In this study, we developed a novel droplet digital PCR (ddPCR) assay targeting the PCV3 cap gene to improve the sensitivity of PCV3 detection. The established assay is highly specific to PCV3, and does not cross react with other important swine pathogens. The assay's detection limit was 1.68 ± 0.29 copies of PCV3 DNA per reaction (n = 8), an approximately 10-fold greater sensitivity than that of our previously developed quantitative real-time PCR (qPCR) assay for the same virus. The ddPCR assay results were highly reproducible, with intra- and inter-assay coefficient of variation values of
Source: Molecular and Cellular Probes - November 21, 2018 Category: Molecular Biology Source Type: research

Development of real-time PCR assays for single and simultaneous detection of infectious bursal disease virus and chicken anemia virus
Publication date: Available online 14 November 2018Source: Molecular and Cellular ProbesAuthor(s): Claudia Techera, Gonzalo Tomás, Yanina Panzera, Alejandro Banda, Paula Perbolianachis, Ruben Pérez, Ana MarandinoAbstractInfectious bursal disease virus (IBDV) and chicken anemia virus (CAV) cause relevant immunosuppressive diseases in poultry. Clinical diagnosis of these viruses is challenging given the different disease presentations and the frequent occurrence of co-infections with other pathogens. Here, we standardized and validated simplex and duplex RT-qPCR assays for the straightforward detection of IBDV ...
Source: Molecular and Cellular Probes - November 15, 2018 Category: Molecular Biology Source Type: research

Genes and transcription factors related to the adverse effects of maternal type I diabetes mellitus on fetal development
ConclusionsSome genes (e.g. CXCL12 and CCL5) and their TFs were significantly and abnormally regulated in the umbilical cord tissue from the pregnancies of T1DM mothers compared to that from non-T1DM mothers. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 15, 2018 Category: Molecular Biology Source Type: research

Rapid and sensitive detection of Amphidinium carterae by loop-mediated isothermal amplification combined with a chromatographic lateral-flow dipstick
In conclusion, the developed LAMP-LFD assay can be used as a reliable and simple method of detecting A. carterae. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 11, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: December 2018Source: Molecular and Cellular Probes, Volume 42Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - November 11, 2018 Category: Molecular Biology Source Type: research

Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR
In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. The coefficients of variation were less than 1.09% in either intra- or inter-assays, indicating high reproducibility. By using this method, the prevalence of BFDV in China was evaluated. 56 feces samples were collected from four psittacine birds breeding facilities in China. The results showed 28 out of 56 samples were positive for BFDV in Real-Time PCR assay, while only 19 samples were positive in PCR a...
Source: Molecular and Cellular Probes - November 9, 2018 Category: Molecular Biology Source Type: research

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3
In conclusion, these assays offer an efficient and reliable tool for identification and subtyping of influenza A virus (subtype H1 and H3) in the resource-limited setting. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - October 27, 2018 Category: Molecular Biology Source Type: research

Development of double labeling in situ hybridization using RNA probes for genome detection of nervous necrosis virus (NNV)
The objective of this study was to develop RNA-ISH using RNA probes for the detection of NNV in infects SSN-1 cells or sevenband grouper Hyporthodus septemfasciatus. Two viral RNA segments viz., RNA1 and RNA2 were synthesized by in vitro transcription and labeled with fluorescein UTP and dignoxigenin dUTP, respectively. These labeled RNA probes specifically detected NNV in infected SSN-1 cells. We also applied double labeling RNA-ISH with two-color staining of RNA probes. The results showed that these two viral genomic segments were localized in same regions although RNA1 was also expressed separately. These findings s...
Source: Molecular and Cellular Probes - October 20, 2018 Category: Molecular Biology Source Type: research

Bta-miR-130a/b regulates preadipocyte differentiation by targeting PPARG and CYP2U1 in beef cattle
This study shows that bta-miR-130a and bta-miR-130b play similar roles in the regulation of adipocyte differentiation in beef muscles by targeting the 3‘UTR of PPARG and CYP2U1. Our result provides a reference for illustrating how noncoding RNAs affects beef quality traits in cattle. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - October 17, 2018 Category: Molecular Biology Source Type: research

In silico investigation of the prion protein glycosylation profiles in relation to scrapie disease resistance in domestic sheep (Ovis aries)
In this study, the potential N- and O-glycosylation positions of sheep prion protein polymorphisms were analyzed, the secondary and three-dimensional protein structure models were predicted, three-dimensional glycoprotein models were constructed and the role of glycosylation positions in protein interactions was investigated. Here, we found that protein secondary and three-dimensional structures vary among polymorphisms. Moreover, we found wild-type prion and all polymorphic variants show N-glycosylation at Asn184 and Asn200 positions, while O-glycosylation profiles are variant-specific. We also found that structural chang...
Source: Molecular and Cellular Probes - October 5, 2018 Category: Molecular Biology Source Type: research

One-step multiplex real-time RT-PCR for detection and typing of dengue virus
In this study, we developed a TaqMan probe-based, one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) system for detection and serotyping DENV. Our linear dynamic range (101 to 107 copies/reaction) showed the R2 values of DENV-1, 2, 3 and 4 as 0.998, 0.998, 0.994, and 0.998, respectively. The detection limits of DENV-1, 2, 3, and 4, were 10 copies/reaction, 100 copies/reaction, 10 copies/reaction, and 100 copies/reaction, respectively. Specificity test results indicated that this system is specific for DENV-1, 2, 3, and 4 and does not react with other viruses. Finally, we validated our results with ...
Source: Molecular and Cellular Probes - October 5, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: October 2018Source: Molecular and Cellular Probes, Volume 41Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - September 21, 2018 Category: Molecular Biology Source Type: research

Construction and rescue of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box deletion within the left terminal region
This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - September 19, 2018 Category: Molecular Biology Source Type: research

Immunopotentiating effect of Inonotus obliquus fermentation products administered at vaccination in chickens
Publication date: Available online 15 September 2018Source: Molecular and Cellular ProbesAuthor(s): Lin Zhang, Dongmei Lin, Haiyan Li, Sen Yu, Junping Bai, Zhiyong Ding, Jiaqiang WuAbstractVaccination is an important approach for the control of avian viral diseases. The aim of this study was to evaluate the immune-potentiating effect of oral administration of Inonotus obliquus fermentation products (IOFP) at vaccination in chickens. In total, 120 one-day-old specific-pathogen-free chickens were randomly assigned to six groups: groups 1 to 3 were vaccinated with Newcastle disease virus (NDV) LaSota live vaccine via intranas...
Source: Molecular and Cellular Probes - September 17, 2018 Category: Molecular Biology Source Type: research

Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis
This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5.14 (P = 0.001) and 2.41 (P = 0.097) times higher in animals with mastitis compared to healt...
Source: Molecular and Cellular Probes - September 12, 2018 Category: Molecular Biology Source Type: research

Development of propidium monoazide–recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae
In this study, a double recombinase polymerase (RPA) amplification assay was developed to rapidly detect these pathogens. Specificity tests revealed that the GAS and GBS strains were positive for speB and SIP genes, respectively. In clinical samples, the double assay performed similarly to the traditional biochemical method. The limits of detection were both ≤100 copies per reaction. In tests for simulant-contaminated samples, bacterial-culture media containing 103 CFU/mL original concentrations of S. pyogenes and S. agalactiae were positive in RPA assays after incubating for 4 h. Results can be obtained at 37 &de...
Source: Molecular and Cellular Probes - August 29, 2018 Category: Molecular Biology Source Type: research

Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus
Publication date: Available online 26 August 2018Source: Molecular and Cellular ProbesAuthor(s): Lei Ma, Feng Cong, Yujun Zhu, Miaoli Wu, Fengjiao Xu, Ren Huang, Robert J. Moore, Pengju GuoAbstractTheiler's murine encephalomyelitis virus (TMEV) is one of the most common viral pathogens that circulate widely in captive mouse colonies. A molecular biology detection method would be a useful tool to use in an integrated program to monitor and prevent TMEV infection and transmission. Thus, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. The sensitivity of the R...
Source: Molecular and Cellular Probes - August 27, 2018 Category: Molecular Biology Source Type: research

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3
In this study, a novel isothermal assay for detecting BPIV3 was established. The method includes a lateral flow dipstick (LFD) assay combined with reverse transcription recombinase polymerase amplification (RT-RPA). First, the analytical sensitivity and specificity of BPIV3 LFD RT-RPA were tested. The LFD RT-RPA assay has a detection limit of up to 100 copies per reaction in 30 min at 38 °C. Then the performance of LFD RT-RPA was evaluated using 95 clinical samples. Compared to qPCR, the LFD RT-RPA assay showed a clinical sensitivity of 94.74%, a clinical specificity of 96.05% and 0.8734 kappa coefficient. These ...
Source: Molecular and Cellular Probes - August 21, 2018 Category: Molecular Biology Source Type: research

Development and validation of a TTR-specific copy number screening tool, and application to potentially relevant patient cohorts
Publication date: Available online 21 August 2018Source: Molecular and Cellular ProbesAuthor(s): Amir Jahic, Andrea Bock, Franz Duca, Diana Bonderman, Julia Mascherbauer, Reinhard Windhager, Michaela Auer-Grumbach, Christian BeetzAbstractTTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are present (ATTRmutant or ATTRm), or acquired when no TTR aberrations are detec...
Source: Molecular and Cellular Probes - August 21, 2018 Category: Molecular Biology Source Type: research

A real time high-resolution melting PCR assay for detection and differentiation among sheep pox virus, goat pox virus, field isolates and vaccine strains of lumpy skin disease virus
Publication date: Available online 10 August 2018Source: Molecular and Cellular ProbesAuthor(s): Yana Pestova, Olga Byadovskaya, Alexander Kononov, Alexander SpryginAbstractIn this paper, we report on the development of a real time high-resolution melting (HRM) PCR assay for detection and differentiation among sheep pox virus (SPPV), goat pox virus (GTPV), field isolates and vaccine strains of lumpy skin disease virus (LSDV) based on high-resolution melting curve analysis of their target PCR amplicons. A 111 bp region of LSDV010 ORF, which harbors unique genetic differences for each of these viral species, was selected as ...
Source: Molecular and Cellular Probes - August 10, 2018 Category: Molecular Biology Source Type: research

Validation of high-resolution melting analysis as a diagnostic tool for endothelin receptor B mutation in American paint horses and allele frequency estimation
Publication date: Available online 8 August 2018Source: Molecular and Cellular ProbesAuthor(s): Peres Ramos Badial, Raffaella Bertoni Cavalcanti Teixeira, Diego José Zanzarini Delfiol, Ligia Souza Lima Silveira da Mota, Alexandre Secorun BorgesAbstractOvero lethal white foal syndrome (OLWFS) is a genetic disorder caused by a dinucleotide mutation in the endothelin receptor type B (EDNRB) gene leading to the death of affected foals shortly after birth. The use of rapid and reliable genetic testing is imperative for the early diagnosis of the mutation avoiding, therefore, either additional suffering or the production ...
Source: Molecular and Cellular Probes - August 8, 2018 Category: Molecular Biology Source Type: research

Inhibition of C5a prevents IL-1β-induced alternations in rat synoviocytes in vitro
Publication date: Available online 6 August 2018Source: Molecular and Cellular ProbesAuthor(s): Wei Lu, Lin Wang, Jing Yao, Wen Wang, Yu ChenAbstractC5a is an important pro-inflammatory peptide involved in complement activation, membrane attack complex formation, immune cell chemotaxis, and allergic responses. Osteoarthritis is a disease characterized by degenerative changes in articular cartilage. It has recently been found that inflammatory responses play an important role in the pathogenesis of osteoarthritis and also in rheumatoid arthritis, where dysfunctional synoviocytes are involved. We performed a series of studie...
Source: Molecular and Cellular Probes - August 6, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: August 2018Source: Molecular and Cellular Probes, Volume 40Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 30, 2018 Category: Molecular Biology Source Type: research

Special Section “Selected topics from The German Human Genetics conference 2017”
Publication date: August 2018Source: Molecular and Cellular Probes, Volume 40Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 30, 2018 Category: Molecular Biology Source Type: research

Highly selective and sensitive detection of Staphylococcus aureus with gold nanoparticle-based core-shell nano biosensor
Publication date: Available online 25 July 2018Source: Molecular and Cellular ProbesAuthor(s): Reza Shahbazi, Mojtaba Salouti, Bahram Amini, Ahmad Jalilvand, Ebrahim Naderlou, Ali Amini, Arash ShamsAbstractStaphylococcus aureus is a gram-positive and opportunistic pathogen that is one of the most common causes of nosocomial infections; therefore, its rapid diagnosis is important and valuable. Today, the use of nanoparticles is expanding due to their unique properties. The purpose of the present study is the determination of S. aureus by a colorimetric method based on gold nanoparticles (AuNPs). Firstly, S. aureus was cultu...
Source: Molecular and Cellular Probes - July 25, 2018 Category: Molecular Biology Source Type: research

The contribution of GTF2I haploinsufficiency to Williams syndrome
Publication date: Available online 3 January 2018Source: Molecular and Cellular ProbesAuthor(s): Thanathom Chailangkarn, Chalongrat Noree, Alysson R. MuotriAbstractWilliams syndrome (WS) is a neurodevelopmental disorder involving hemideletion of as many as 26–28 genes, resulting in a constellation of unique physical, cognitive and behavior phenotypes. The haploinsufficiency effect of each gene has been studied and correlated with phenotype(s) using several models including WS subjects, animal models, and peripheral cell lines. However, links for most of the genes to WS phenotypes remains unclear. Among those genes, g...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Modeling rare diseases with induced pluripotent stem cell technology
Publication date: Available online 5 January 2018Source: Molecular and Cellular ProbesAuthor(s): Ruthellen H. Anderson, Kevin R. FrancisAbstractRare diseases, in totality, affect a significant proportion of the population and represent an unmet medical need facing the scientific community. However, the treatment of individuals affected by rare diseases is hampered by poorly understood mechanisms preventing the development of viable therapeutics. The discovery and application of cellular reprogramming to create novel induced pluripotent stem cell models of rare diseases has revolutionized the rare disease community. Through...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Peroxisomal disorders: Improved laboratory diagnosis, new defects and the complicated route to treatment
Publication date: Available online 10 February 2018Source: Molecular and Cellular ProbesAuthor(s): Ronald J.A. WandersAbstractPeroxisomes catalyze a number of essential metabolic functions of which fatty acid alpha- and beta-oxidation, ether phospholipid biosynthesis, glyoxylate detoxification and bile acid synthesis are the most important. The key role of peroxisomes in humans is exemplified by the existence of a group of peroxisomal disorders, caused by mutations in > 30 different genes which code for proteins with a role in either peroxisome biogenesis or one of the metabolic pathways in peroxisomes. Technologica...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Colorimetric detection of microRNA based on DNAzyme and nuclease-assisted catalytic hairpin assembly signal amplification
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju, Jiayu WanAbstractAccurate and quantitative analysis of microRNA (miRNA) expression is critical for the diagnostics and theranostics of a disease. Herein, a proof-of-concept of a colorimetric horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) biosensor for miRNA assay based on nuclease-assisted catalytic hairpin assembly (CHA) signal amplification was demonstrated. Duplex-specific nuclease (DSN) was employed to cleave the single-stranded DNA (ssDNA) chimeric probe (CP) ...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5
In this study, we focused on the roles of OGG1 in histone modification. First, to study the effects of OGG1 on histone modification, the protein levels of symmetric dimethylation of histone H4 arginine-3 (H4R3me2s) were determined by western blot analysis following the knockdown or overexpression of OGG1. Second, the molecular mechanisms by which OGG1 regulates H4R3me2s were assessed by co-immunoprecipitation (CO-IP) assays in mouse embryonic fibroblast (MEF) wild-type (WT) and Ogg−/− cells. Finally, to verify the regulation of H4R3me2s by OGG1 on specific genes, chromatin immunoprecipitation (CHIP) was perform...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Structure-activity modelling of essential oils, their components, and key molecular parameters and descriptors
This study aimed to elucidate SARs of essential oil components from experimental and literature sources. Minimum Inhibitory Concentrations (MICs) of essential oil components were determined against Escherichia coli and Staphylococcus aureus using a microdilution method and then compared to those in published in literature. Of 12 essential oil components tested, carvacrol and cuminaldehyde were most potent with MICs of 1.98 and 2.10 mM, respectively. The activity of 21 compounds obtained from the literature, MICs ranged from 0.004 mM for limonene to 36.18 mM for α-terpineol. A 3D qualitative SAR model was genera...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus
In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. RPA primers and LF probes were designed by targeting the specific G gene, and the amplification product can be visualized on a simple lateral flow dipstick with the naked eyes. The amplification reaction was performed at 38 °C for 20 min and LFD incubation time within 5 min. The detection limit of this assay was 8 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses such as bovine viral diarrhea virus, infectious bovine ...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Protective effect of berberine against cardiac ischemia/reperfusion injury by inhibiting apoptosis through the activation of Smad7
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Weidong Yao, Xin Wang, Kun XiaoAbstractBerberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cel...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of a SYBR Green I real-time PCR for detection and quantitation of orthopoxvirus by using Ectromelia virus
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Wenyu Cheng, Xiaobing He, Huaijie Jia, Guohua Chen, Cong Wang, Jun Zhang, Zhizhong JingAbstractEctromelia virus (ECTV) is the causative agent of mousepox, which has devastating effects in laboratory-mouse colonies and causes economic loss in biomedical research. More importantly, ECTV has been extensively used as an excellent model for studies of the pathogenesis and immunobiology of human smallpox. A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by u...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development of HyBeacon® probes for specific mRNA detection using body fluids as a model system
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Beccy Stafford-Allen, Nick Dawnay, Erin K. Hanson, Glyn Ball, Ambika Gupta, Stephen Blackman, David J. French, Nicola Duxbury, Jack Ballantyne, Simon WellsAbstractHyBeacons are linear oligonucleotides which incorporate fluorescent dyes covalently linked to internal nucleotides. They have previously been used with PCR and isothermal amplification to interrogate SNPs and STRs in fields as diverse as clinical diagnostics, food authentication, and forensic DNA profiling. This work explores their use for the identification of expressed gene s...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

WITHDRAWN: Designing and computational validation of microarray probe sets for the diagnosis of viruses of non-human vertebrates
In this study, a microarray probe dataset was strategized exclusively targeting the diagnosis of viruses infecting non-human vertebrates. The dataset contains 21,234 species-specific probes from 766 pathogenic virus species and subtypes and 3998 genus level conserved probes from 146 virus genera. The probes were designed from coding regions of virus genomes and selected after rigorous screening for species specificities, minimal cross-reactivity and thermodynamic properties. The probe sets were computationally compared with the probes of earlier published pan-viral arrays; GreeneChipPm, ViroChip and Microbial Detection Arr...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Development and validation of pan-Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens
ConclusionThe findings suggest that the developed Taqman qPCR assay is sensitive, specific and has potential to be applied in a larger subsequent study. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
Publication date: April 2018Source: Molecular and Cellular Probes, Volume 38Author(s): Yaru Sun, Yuening Cheng, Peng Lin, Li Yi, Mingwei Tong, Zhigang Cao, Gaili Wang, Shuang Li, Shipeng Cheng, Wanzhe Yuan, Jianke WangAbstractCanine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), w...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method
Publication date: Available online 22 May 2018Source: Molecular and Cellular ProbesAuthor(s): Zhiqing Huang, Jianwei Zheng, Chunmei Shi, Qiang ChenAbstractCoupling propidium monoazide (PMA) with quantitative PCR (PMA-qPCR) has been successfully applied to specific detection and quantification of viable cells in various samples. The optimal PMA treatment condition is usually determined through qPCR. However, it is a tedious, time consuming and costly process including DNA extraction and qPCR. To overcome this problem, a flow cytometry-based (FCM-based) method was first proposed in this study to replace qPCR for screening of...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

The development and application of a duplex reverse transcription loop-mediated isothermal amplification assay combined with a lateral flow dipstick method for Macrobrachium rosenbergii nodavirus and extra small virus isolated in China
In conclusion, a convenient, sensitive and selective practical molecular diagnostic method was developed with the potential for diagnosis and prevention of WTD. (Source: Molecular and Cellular Probes)
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Identification of bta-miR-15a∼16a cluster expression, localization and regulated target in Holsteins
In this study, we investigated the expression pattern and tissue localization of bta-miR-15a and bta-miR-16a. The expression levels of bta-miR-15a and bta-miR-16a were significantly upregulated in mammary tissues and blood neutrophils of mastitis-infected cows, compared with those of healthy cows (P 
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

A novel HRM assay for differentiating classical strains and highly pathogenic strains of type 2 porcine reproductive and respiratory syndrome virus
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Junying Sun, Gali Bingga, Zhicheng Liu, Chunhong Zhang, Haiyan Shen, Pengju Guo, Jianfeng ZhangAbstractDifferentiation of classical strains and highly pathogenic strains of porcine reproductive and respiratory syndrome virus is crucial for effective vaccination programs and epidemiological studies. We used nested PCR and high resolution melting curve analysis with unlabeled probe to distinguish between the classical and the highly pathogenic strains of this virus. Two sets of primers and a 20 bp unlabeled probe were designed from the NSP3...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Changes in gene expression in lungs of mice exposed to traffic-related air pollution
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Jie Yang, Yi Chen, Zhi Yu, Hui Ding, Zhongfu MaAbstractLong-term exposure to traffic-related pollutants can lead to a variety of respiratory diseases, including inflammation, asthma, and lung cancer; however, the underlying biological mechanisms are not fully understood. We focused on the effects of exposure to different air pollutants on the expression of genes associated with inflammatory immune responses, allergic reactions and asthma, and lung cancer. In order to understand the cellular responses induced by exposure to different traff...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Libing Liu, Jianchang Wang, Yunyun Geng, Jinfeng Wang, Ruiwen Li, Ruihan Shi, Wanzhe YuanAbstractA visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could de...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research

CRISPR-Cas9; an efficient tool for precise plant genome editing
Publication date: June 2018Source: Molecular and Cellular Probes, Volume 39Author(s): Waqar IslamAbstractEfficient plant genome editing is dependent upon induction of double stranded DNA breaks (DSBs) through site specified nucleases. These DSBs initiate the process of DNA repair which can either base upon homologous recombination (HR) or non-homologous end jointing (NHEJ). Recently, CRISPR-Cas9 mechanism got highlighted as revolutionizing genetic tool due to its simpler frame work along with the broad range of adaptability and applications. So, in this review, I have tried to sum up the application of this biotechnologica...
Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research