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Specialty: Biochemistry
Condition: Brain Tumor

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Total 34 results found since Jan 2013.

Cell-penetrating peptides for siRNA delivery to glioblastomas
In conclusion, we have established an efficient non-covalently complexed carrier (PF14:TG1) for siRNA delivery to human glioblastoma cells (U87), showing a significant two-fold increase in gene-silencing efficiency compared to the parent peptide PF14 and also improved specificity to U87 cells compared to non-glioma targeted cells. Graphical abstract
Source: Peptides - May 1, 2018 Category: Biochemistry Source Type: research

Hydroxyl-Rich PGMA-Based Cationic Glycopolymers for Intracellular siRNA Delivery: Biocompatibility and Effect of Sugar Decoration Degree.
Abstract The ErbB family of proteins, structurally related to the epidermal growth factor receptor (EGFR), is found to be over-expressed in many cancers such as gliomas, lung and cervical carcinomas. Gene therapy allows to modify the expression of genes like ErbB and has been a promising strategy to target oncogenes and tumor suppressor genes. In the current work, novel hydroxyl-rich polyglycidyl methacrylate (PGMA)-based cationic glycopolymers were designed for intracellular small interfering RNA (siRNA) delivery to silence the EGFR gene. The cationic polymers with different sugar decoration degrees (0%, 9%, and ...
Source: Biomacromolecules - April 9, 2019 Category: Biochemistry Authors: Chen Y, Diaz-Dussan D, Peng YY, Narain R Tags: Biomacromolecules Source Type: research

siRNA targeting stathmin inhibits invasion and enhances chemotherapy sensitivity of stem cells derived from glioma cell lines.
Abstract Glioma is one of the most highly angiogenic tumors, and glioma stem cells (GSCs) are responsible for resistance to chemotherapy and radiotherapy, as well as recurrence after operation. Stathmin is substantial for mitosis and plays an important role in proliferation and migration of glioma-derived endothelial cells. However, the relationship between stathmin and GSCs is incompletely understood. Here we isolated GSCs from glioma cell lines U87MG and U251, and then used siRNA targeting stathmin for silencing. We showed that silencing of stathmin suppressed the proliferation, increased the apoptosis rate, and...
Source: Acta Biochimica et Biophysica Sinica - October 27, 2014 Category: Biochemistry Authors: Song Y, Mu L, Han X, Liu X, Fu S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells.
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta - January 8, 2018 Category: Biochemistry Authors: Ohlig T, Le DV, Gardemann A, Wolke C, Gürtler S, Peter D, Schild L, Lendeckel U Tags: Biochim Biophys Acta Source Type: research

Silencing of epidermal growth factor, latrophilin and seven transmembrane domain-containing protein 1 (ELTD1) via siRNA induced cell death in glioblastoma.
In this study we aim to analyse whether this receptor may be used as a target molecule in glioblastoma therapy. Our results showed that small interfering RNA silencing ELTD1 caused cytotoxicity in glioblastoma cells. We also found that PDGFR, VEGFR and their common PI3K/mTOR intracellular pathway inactivation induced cytotoxicity in glioblastoma cells. Further, we found high percent of cytotoxicity in a low passage glioblastoma cell line after BEZ235 (a dual inhibitor of PI3K/mTOR pathway) treatment at nanomolar concentrations, compared to AG1433 (a PDGFR inhibitor) and SU1498 (a VEGFR inhibitor) that were only cytotoxic a...
Source: Journal of Immunoassay and Immunochemistry - July 6, 2016 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research

P53 induction accompanying G2/M arrest upon knockdown of tumor suppressor HIC1 in U87MG glioma cells.
In this study, we investigated the role of HIC1 in cell cycle and proliferation of glioma cell line U87MG which has wild type p53, in both serum-containing and serum-deprived medium. Microscopic analysis and MTT assay showed reduced cell number and rate of proliferation upon HIC1 knock down compared to control siRNA (p = 0.025) and untreated cells (p = 0.03) in serum-containing medium and serum-free medium (p = 0.014 vs control siRNA; p = 0.018 vs untreated cells). Cell cycle analysis revealed an arrest at G2/M phase of cell cycle with no demonstrable increase in apoptosis with both medium. An increased expression ...
Source: Molecular and Cellular Biochemistry - July 4, 2014 Category: Biochemistry Authors: Kumar S Tags: Mol Cell Biochem Source Type: research

TGF-β-induced hCG-β regulates redox homeostasis in glioma cells.
Abstract Transforming growth factor (TGF-β) is associated with the progression of glioblastoma multiforme (GBM)-the most malignant of brain tumors. Since there is a structural homology between TGF-β and human chorionic gonadotropin (hCG) and as both TGF-β and hCG-β are known regulators of oxidative stress and survival responses in a variety of tumors, the role of TGF-β in the regulation of hCG-β and its consequences on redox modulation of glioblastoma cells was investigated. A heightened hCG-β level was observed in GBM tumors. TGF-β treatment increased hCG-β expression in glioma cell lines, and this heigh...
Source: Molecular and Cellular Biochemistry - October 10, 2014 Category: Biochemistry Authors: Ahmad F, Ghosh S, Sinha S, Joshi SD, Mehta VS, Sen E Tags: Mol Cell Biochem Source Type: research

Frequent Nek1 overexpression in human gliomas.
Abstract Never in mitosis A (NIMA)-related kinase 1 (Nek1) regulates cell cycle progression to mitosis. Its expression and potential functions in human gliomas have not been studied. Here, our immunohistochemistry (IHC) assay and Western blot assay results showed that Nek1 expression was significantly upregulated in fresh and paraffin-embedded human glioma tissues. Its level in normal brain tissues was low. Nek1 overexpression in human gliomas was correlated with the proliferation marker (Ki-67), tumor grade, Karnofsky performance scale (KPS) and more importantly, patients' poor survival. Further studies showed th...
Source: Biochemical and Biophysical Research communications - May 28, 2016 Category: Biochemistry Authors: Zhu J, Cai Y, Liu P, Zhao W Tags: Biochem Biophys Res Commun Source Type: research

G9a inhibition induced PKM2 regulates autophagic responses.
Abstract Epigenetic regulation by histone methyltransferase G9a is known to control autophagic responses. As the link between autophagy and metabolic homeostasis is widely accepted, we investigated whether G9a affects metabolic circuitries to affect autophagic response in glioma cells. Both pharmacological inhibition and siRNA mediated knockdown of G9a increased autophagy marker LC3B in glioma cells. G9a inhibitor BIX-01294 (BIX) induced Akt-dependent increase in HIF-1α expression and activity. Inhibition of Akt-HIF-1α axis reversed BIX-mediated (i) increase in LC3B expression and (ii) decrease in Yes-associated...
Source: The International Journal of Biochemistry and Cell Biology - July 10, 2016 Category: Biochemistry Authors: Ahmad F, Dixit D, Joshi SD, Sen E Tags: Int J Biochem Cell Biol Source Type: research

MicroRNA-222 promotes tumorigenesis via targeting DKK2 and activating the Wnt/β-catenin signaling pathway
Highlights: Abstract: MiR-222 in glioma can regulate cell cycle progression and apoptosis. However, the relationship between miR-222 and Wnt/β-catenin signaling pathway in glioma remains unknown. Here, we found that the Dickkopf-2 gene (DKK2) was a direct target of miR-222 by target prediction analysis and dual luciferase reporter assay. RNA interference silencing of DKK2 proved that miR-222 overexpression led to constitutive activation of β-catenin through inhibition of DKK2 expression in glioma cells. Furthermore, miR-222 siRNA significantly inhibited tumorigenesis in vivo. Finally, Western blot analysis showed that mi...
Source: FEBS Letters - April 15, 2013 Category: Biochemistry Authors: Qifeng Li, Ke Shen, Yang Zhao, Xiaoguang He, Chenkai Ma, Lin Wang, Baocheng Wang, Jianwen Liu, Jie Ma Tags: Research Letters Source Type: research

Human umbilical cord mesenchymal stem cells inhibit C6 glioma growth via secretion of dickkopf-1 (DKK1).
Abstract Mesenchymal stem cells (MSCs) represent a potential therapeutic target for glioma. We determined the molecular mechanism of inhibitory effect of human umbilical cord-derived MSCs (hUC-MSCs) on the growth of C6 glioma cells. We demonstrated that hUC-MSCs inhibited C6 cell growth and modulated the cell cycle to G0/G1 phase. The expression of β-catenin and c-Myc was downregulated in C6 cells by conditioned media from hUC-MSCs, and the levels of secreted DKK1 were positively correlated with concentrations of hUCMSCs-CM. The inhibitory effect of hUC-MSCs on C6 cell proliferation was enhanced as the concentrat...
Source: Molecular and Cellular Biochemistry - October 9, 2013 Category: Biochemistry Authors: Ma S, Liang S, Jiao H, Chi L, Shi X, Tian Y, Yang B, Guan F Tags: Mol Cell Biochem Source Type: research

Inhibition of autophagy enhances apoptosis induced by proteasome inhibitor bortezomib in human glioblastoma U87 and U251 cells.
Abstract Glioblastoma is the most aggressive cerebral gliomas. Despite advances in therapies, the prognosis is still very poor. Therefore, novel therapeutic strategies are required. As a proteasome inhibitor, bortezomib has shown its efficacy as an active antitumor agent against a variety of tumors. However, inhibition of proteasome activity leads to cell death and also induces cell autophagy, and due to the dual roles of autophagy in the survival and death of tumor cells, the effect of inhibition of autophagy on glioblastoma cells remains to be explored. We therefore assessed whether bortezomib is capable of indu...
Source: Molecular and Cellular Biochemistry - October 9, 2013 Category: Biochemistry Authors: Zhang X, Li W, Wang C, Leng X, Lian S, Feng J, Li J, Wang H Tags: Mol Cell Biochem Source Type: research

CXCL12-induced upregulation of FOXM1 expression promotes human glioblastoma cell invasion.
In this study, we demonstrate that CXCL12 increases the production of FOXM1 by binding to CXCR4 in GBM cell lines. Furthermore, pretreatment with an inhibitor of the PI3K/AKT pathway abrogated the CXCL12-induced expression of FOXM1. In addition, there was a positive correlation between CXCL12/CXCR4 expression and FOXM1 expression in human malignant glioma tissues. Finally, a functional assay revealed that CXCL12 does not stimulate GBM cell invasion when FOXM1 expressionis silenced using a small interfering RNA (siRNA). Collectively, these findings suggest that CXCL12 promotes GBM cell invasion in part byincreasing the expr...
Source: Biochemical and Biophysical Research communications - February 19, 2014 Category: Biochemistry Authors: Wang S, Zhang S, Li J, Xu X, Weng Y, Zheng M, Ouyang L, Li F Tags: Biochem Biophys Res Commun Source Type: research

MiR-224 expression increases radiation sensitivity of glioblastoma cells.
Abstract Glioblastoma (GBM) is the most common and highly aggressive primary malignant brain tumor. The intrinsic resistance of this brain tumor limits the efficacy of administered treatment like radiation therapy. In the present study, effect of miR-224 on growth characteristics of established GBM cell lines was analyzed. MiR-224 expression in the cell lines as well as primary GBM tumor tissues was found to be low. Exogenous transient expression of miR-224 using either synthetic mimics or stable inducible expression using doxycycline inducible lentiviral vector carrying miR-224 gene, was found to bring about 30-5...
Source: Biochemical and Biophysical Research communications - April 29, 2014 Category: Biochemistry Authors: Upraity S, Shaikh S, Padul V, Shirsat NV Tags: Biochem Biophys Res Commun Source Type: research

Ouabain elicits human glioblastoma cells apoptosis by generating reactive oxygen species in ERK-p66SHC-dependent pathway.
Abstract Excessive reactive oxygen species (ROS) generation has been implicated as one of main agents in ouabain-induced anticancer effect. Unfortunately, the signaling pathways under it are not very clarified. In the present study, we investigated the molecular mechanism involved in ouabain-induced ROS generation and cell apoptosis on human U373MG and U87MG glioma cells. Ouabain-induced glioblastoma cells apoptosis and increased ROS generation. Clearance ROS by three different ROS scavenger partly, but not totally, reversed ouabain's effect on cell apoptosis. Ouabain-induced ROS generation was not regulated by ca...
Source: Molecular and Cellular Biochemistry - September 13, 2014 Category: Biochemistry Authors: Yan X, Liang F, Li D, Zheng J Tags: Mol Cell Biochem Source Type: research