• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Increased SPHK2 transcription of human colon cancer cells in serum‐depleted culture: the involvement of CREB transcription factor
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - March 24, 2015 Category: Biochemistry Authors: Naoki Mizutani, Yukari Omori, Koji Tanaka, Hiromi Ito, Akira Takagi, Tetsuhito Kojima, Masahiro Nakatochi, Hideo Ogiso, Yoshiyuki Kawamoto, Mitsuhiro Nakamura, Motoshi Suzuki, Mamoru Kyogashima, Keiko Tamiya‐Koizumi, Yoshinori Nozawa, Takashi Murate Tags: Article Source Type: research

Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy
In this study, thiol-modified siRNA (SH-siRNA) was delivered by bioreducible polyethylenimine (ssPEI), to enhance physicochemical properties of polyplexes and function of siRNA through disulfide bonding between SH-siRNA and ssPEI. The ssPEI was utilized to deliver Akt1 SH-siRNA for suppression of Akt1 mRNA and blockage of Akt1 protein translation, resulting in reduced cellular proliferation and the induction of apoptosis. Disulfide bondings between the ssPEI and SH-siRNA through thiol groups in both were confirmed by DTT treatment. Complexation between ssPEI and Akt1SH-siRNA was enhanced and reduced surface charge of ssPEI...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2015 Category: Biochemistry Source Type: research

CXCL13-CXCR5 axis promotes the growth and invasion of colon cancer cells via PI3K/AKT pathway.
Abstract CXCL13, an inflammatory factor in the microenvironment, plays a vital role in the progression of inflammatory diseases and tumors. CXCL13 and its receptor CXCR5 have been reported to be associated with poor prognosis of advanced colon cancer. However, the molecular mechanisms of CXCL13-CXCR5 axis in colon cancer remain elusive. The aim of this study was to investigate the role of CXCR5-CXCL13 axis in the growth and invasion of colon cancer cells. Our results showed that CXCL13 promoted the growth, migration, and matrigel invasion of colon cancer cells. Furthermore, CXCL13 increased the expression and secr...
Source: Molecular and Cellular Biochemistry - December 5, 2014 Category: Biochemistry Authors: Zhu Z, Zhang X, Guo H, Fu L, Pan G, Sun Y Tags: Mol Cell Biochem Source Type: research

Identification of anti-metastatic drug and natural compound targets in isogenic colorectal cancer cells.
Abstract Therapeutic strategies for cancer treatment often remain challenging due to the cumulative risk derived from metastasis, which has been described as an aggressive state of cancer cell proliferation often resulting in failure of clinical therapy. In the current study, anti-metastatic properties of three chemotherapeutic drugs and three compounds from natural sources were investigated by comparative proteomic analysis. Proteomic profile comparison of the isogenic primary and metastatic colon cancer cell lines SW480 and SW620 identified two potential metastasis related molecular targets: fatty acid synthase ...
Source: Journal of Proteomics - October 23, 2014 Category: Biochemistry Authors: Lee JG, McKinney KQ, Pavlopoulos AJ, Park JH, Hwang S Tags: J Proteomics Source Type: research

Ki-1/57 and CGI-55 ectopic expression impact cellular pathways involved in proliferation and stress response regulation.
Abstract Ki-1/57 (HABP4) and CGI-55 (SERBP1) are regulatory proteins and paralogs with 40.7% amino acid sequence identity and 67.4% similarity. Functionally, they have been implicated in the regulation of gene expression on both the transcriptional and mRNA metabolism levels. A link with tumorigenesis is suggested, since both paralogs show altered expression levels in tumor cells and the Ki-1/57 gene is found in a region of chromosome 9q that represents a haplotype for familiar colon cancer. However, the target genes regulated by Ki-1/57 and CGI-55 are unknown. Here, we analyzed the alterations of the global trans...
Source: Biochimica et Biophysica Acta - September 6, 2014 Category: Biochemistry Authors: Costa FC, Saito A, Gonçalves KA, Vidigal PM, Meirelles GV, Bressan GC, Kobarg J Tags: Biochim Biophys Acta Source Type: research

B4GALNT2 gene expression controls the biosynthesis of Sd(a) and sialyl Lewis X antigens in healthy and cancer human gastrointestinal tract.
Abstract The histo blood group carbohydrate Sd(a) antigen and its cognate biosynthetic enzyme B4GALNT2 show the highest level of expression in normal colon. Their dramatic down regulation previously observed in colon cancer tissues could play a role in the concomitant elevation of the selectin ligand sLe(x), involved in metastasis. However, down regulation of sLe(x) expression by B4GALNT2 has been so far demonstrated in vitro, but not in tissues. The human B4GALNT2 gene specifies at least two transcripts, diverging in the first exon, never studied in normal and cancer tissues. The long form contains a 253 nt exon ...
Source: The International Journal of Biochemistry and Cell Biology - June 19, 2014 Category: Biochemistry Authors: Groux-Degroote S, Wavelet C, Krzewinski-Recchi MA, Portier L, Mortuaire M, Mihalache A, Trinchera M, Delannoy P, Malagolini N, Chiricolo M, Dall'Olio F, Harduin-Lepers A Tags: Int J Biochem Cell Biol Source Type: research

Amphipathic Homopolymers for siRNA Delivery: Probing Impact of Bifunctional Polymer Composition on Transfection.
In this study, we systematically explore the influence of the lipophilic group on the siRNA transfection properties of the polycationic-based delivery vectors. For this, a novel and modular synthetic strategy was developed for the preparation of polymers carrying a cationic site and a lipophilic group at each polymer repeat unit. These bifunctional polymers could form a complex with siRNA and deliver it to human colon carcinoma cells (HT-29-luc). In general, transfection capability increased with an increase in the chain length of the lipophilic moiety. The best transfection agent, a polymer containing ammonium groups and ...
Source: Biomacromolecules - April 22, 2014 Category: Biochemistry Authors: Buerkli C, Lee SH, Moroz E, Stuparu MC, Leroux JC, Khan A Tags: Biomacromolecules Source Type: research

End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis
Highlights: Abstract: End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa (“field effect”) of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. T...
Source: FEBS Letters - February 3, 2014 Category: Biochemistry Authors: Yolanda Stypula-Cyrus, Nikhil N. Mutyal, Mart Dela Cruz, Dhananjay P. Kunte, Andrew J. Radosevich, Ramesh Wali, Hemant K. Roy, Vadim Backman Tags: Research Letters Source Type: research

Combined incubation of colon carcinoma cells with phorbol ester and mitochondrial uncoupling agents results in synergic elevated reactive oxygen species levels and increased γ-glutamyltransferase expression.
Abstract The NADPH oxidase (NOX) is a significant determinant for the expression and activity of γ-glutamyltransferase (GGT), which is frequently upregulated after increased levels of reactive oxygen species (ROS) and oxidative stress. Earlier studies on human colon carcinoma HT-29 cells have shown that treatment with phorbol 12-myristate 13-acetate (PMA) activates NOX thus increasing the intracellular level of ROS and upregulating GGT. Another important source of cellular ROS is the mitochondria, and treatment with the mitochondria uncoupler carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) results in ...
Source: Molecular and Cellular Biochemistry - November 27, 2013 Category: Biochemistry Authors: Pandur S, Ravuri C, Moens U, Huseby NE Tags: Mol Cell Biochem Source Type: research

Distinct inhibitory efficiency of siRNAs and DNAzymes to β1 integrin subunit in blocking tumor growth.
In this study, we compared inhibitory efficiencies of siRNA and DNAzymes against the β1 integrin subunit (DEβ1), in a mouse xenograft model. Both inhibitors were used under their most favorable conditions, in terms of concentrations, incubation time and lack of cytotoxic effects. Transfection of siRNAβ1 or DEβ1 remarkably inhibited the growth of both PC3 and HT29 colon cancer cells in vitro, and decreased their capability of initiating tumor formation in the mouse xenograft model. siRNAβ1 appeared to be slightly more efficient than DEβ1 when tested in vitro, however it was comparably less proficient in blocking the t...
Source: Acta Biochim Pol - May 23, 2013 Category: Biochemistry Authors: Wiktorska M, Sacewicz-Hofman I, Stasikowska-Kanicka O, Danilewicz M, Niewiarowska J Tags: Acta Biochim Pol Source Type: research

Pages: 1  2  3  4