HSP70 — A Key Regulator in Chondrocyte Homeostasis Under Naturally Coupled Hydrostatic Pressure-Thermal Stimuli
During physical activities, chondrocytes experience coupled stimulation of hydrostatic pressure (HP) and a transient increase in temperature (T), with the latter varying within a physiological range from 32.5 °C to 38.7 °C. Previous short-term in vitro studies have demonstrated that the combined HP-T stimuli more significantly enhance chondroinduction and chondroprotection of chondrocytes than isolated applications. Interestingly, this combined benefit is associated with a corresponding increase in HSP 70 levels when HP and T are combined. (Source: Osteoarthritis and Cartilage)
Source: Osteoarthritis and Cartilage - April 26, 2024 Category: Rheumatology Authors: Yanheng Guo, Theofanis Stampoultzis, Peyman Karami, Naser Nasrollahzadeh, Vijay Kumar Rana, Dominique P. Pioletti Source Type: research
Non-invasive electroarthrography measures cartilage in live horses and correlates to direct measurements of cartilage streaming potentials in weight bearing regions of equine metacarpophalangeal joints
To perform non-invasive Electroarthrography (EAG) on live horses and establish relationships between EAG and direct measurements of cartilage streaming potentials in weight bearing areas of the equine metacarpophalangeal joint. (Source: Osteoarthritis and Cartilage)
Source: Osteoarthritis and Cartilage - April 26, 2024 Category: Rheumatology Authors: Adele Changoor, Martin Garon, Eric Quenneville, Pierre Savard, Michael D. Buschmann, Mark B. Hurtig Source Type: research
Comparative effectiveness of different placebos and comparator groups for hand osteoarthritis exploring the impact of contextual factors: A systematic review and meta-analysis of randomised trials
To examine the pain relief effects of comparators (placebos and untreated control groups) in hand osteoarthritis trials and the impact of contextual factors. (Source: Osteoarthritis and Cartilage)
Source: Osteoarthritis and Cartilage - April 26, 2024 Category: Rheumatology Authors: Ida Maria Balsby, Sabrina M. Nielsen, Robin Christensen, Marius Henriksen, Louise Ulff-M øller Dahl, Johannes Iuel Berg, Simon Tarp, Féline Kroon, Margreet Kloppenburg, Weiya Zhang, David J. Hunter, Henning Bliddal, Anna Døssing Source Type: research
HSP70 —A key regulator in chondrocyte homeostasis under naturally coupled hydrostatic pressure-thermal stimuli
During physical activities, chondrocytes experience coupled stimulation of hydrostatic pressure (HP) and a transient increase in temperature (T), with the latter varying within a physiological range from 32.5 °C to 38.7 °C. Previous short-term in vitro studies have demonstrated that the combined HP-T stimuli more significantly enhance chondroinduction and chondroprotection of chondrocytes than isolated applications. Interestingly, this combined benefit is associated with a corresponding increase i n HSP70 levels when HP and T are combined. (Source: Osteoarthritis and Cartilage)
Source: Osteoarthritis and Cartilage - April 26, 2024 Category: Rheumatology Authors: Yanheng Guo, Theofanis Stampoultzis, Peyman Karami, Naser Nasrollahzadeh, Vijay K. Rana, Dominique P. Pioletti Source Type: research
Patient acceptable symptom state and treatment failure threshold values for work productivity and activity Impairment and EQ-5D-5L in osteoarthritis
ConclusionPASS and TF threshold values for WPAI and EQ-5D-5L might be useful for meaningful interpretation of these measures among people with OA. The observed baseline dependency of estimated thresholds limits their generalizability and values should be applied with great caution in other settings/populations. (Source: Quality of Life Research)
Source: Quality of Life Research - April 25, 2024 Category: Health Management Source Type: research
Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model
In this study, we prepared exosomes (EXOs) from cartilage stem/progenitor cells (CSPCs) and found that treatment with EXOs increased the viability, migration, and proliferation of cultured primary chondrocytes. In a subacute OA rat model, the application of EXOs facilitated cartilage regeneration as evidenced by histological staining. Exosomal protein analysis together with bioinformatics suggested that cyclin-dependent kinase 9 (CDK9) is a key factor for chondrocyte growth and migration. Functional studies confirmed this prediction, that is, inhibiting CDK9 reduced the beneficial effects induced by EXOs in primary chondro...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Jing Chen Xiaohui Ni Jian Yang Hongwei Yang Xiaoyu Liu Minhao Chen Cheng Sun Youhua Wang Source Type: research
CHMP5 attenuates osteoarthritis via inhibiting chondrocyte apoptosis and extracellular matrix degradation: involvement of NF- κB pathway
CONCLUSIONS: This study demonstrated that CHMP5 repressed IL-1β-induced chondrocyte apoptosis and ECM degradation and blocked NF-κB activation. It was shown that CHMP5 might be a novel potential therapeutic target for OA in the future.PMID:38664616 | DOI:10.1186/s10020-024-00819-6 (Source: Molecular Medicine)
Source: Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Weilu Gao Rui Liu Keke Huang Wenhan Fu Anquan Wang Gongwen Du Hao Tang Li Yin Zongsheng S Yin Source Type: research
Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model
In this study, we prepared exosomes (EXOs) from cartilage stem/progenitor cells (CSPCs) and found that treatment with EXOs increased the viability, migration, and proliferation of cultured primary chondrocytes. In a subacute OA rat model, the application of EXOs facilitated cartilage regeneration as evidenced by histological staining. Exosomal protein analysis together with bioinformatics suggested that cyclin-dependent kinase 9 (CDK9) is a key factor for chondrocyte growth and migration. Functional studies confirmed this prediction, that is, inhibiting CDK9 reduced the beneficial effects induced by EXOs in primary chondro...
Source: Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Jing Chen Xiaohui Ni Jian Yang Hongwei Yang Xiaoyu Liu Minhao Chen Cheng Sun Youhua Wang Source Type: research
Human Infrapatellar Fat Pad Mesenchymal Stem Cell-derived Extracellular Vesicles Purified by Anion Exchange Chromatography Suppress Osteoarthritis Progression in a Mouse Model
CONCLUSION: Using an OA mouse model, we found that infrapatellar fat pad-derived MSC-extracellular vesicles could delay OA progression via alleviating pain and suppressing cartilage degeneration, osteophyte formation, and synovial inflammation. The autologous origin of extracellular vesicles and scalable purification method make our strategy potentially viable for clinical translation.CLINICAL RELEVANCE: Infrapatellar fat pad-derived MSC-extracellular vesicles isolated by anion exchange chromatography can suppress OA progression in a mouse model. Further studies with large-animal models, larger animal groups, and subsequen...
Source: Cell Research - April 25, 2024 Category: Cytology Authors: Qisong Liu Jianqun Wu Hua Wang Zhaofeng Jia Guangheng Li Source Type: research
Control of articular degeneration by extracellular vesicles from stem/stromal cells as a potential strategy for the treatment of osteoarthritis
Biochem Pharmacol. 2024 Apr 23:116226. doi: 10.1016/j.bcp.2024.116226. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a degenerative joint condition that contributes to years lived with disability. Current therapeutic approaches are limited as there are no disease-modifying interventions able to delay or inhibit the progression of disease. In recent years there has been an increasing interest in the immunomodulatory and regenerative properties of mesenchymal stem/stromal cells (MSCs) to develop new OA therapies. Extracellular vesicles (EVs) mediate many of the biological effects of these cells and may represent an al...
Source: Biochemical Pharmacology - April 25, 2024 Category: Drugs & Pharmacology Authors: Mar ía José Alcaraz Source Type: research
Human Infrapatellar Fat Pad Mesenchymal Stem Cell-derived Extracellular Vesicles Purified by Anion Exchange Chromatography Suppress Osteoarthritis Progression in a Mouse Model
CONCLUSION: Using an OA mouse model, we found that infrapatellar fat pad-derived MSC-extracellular vesicles could delay OA progression via alleviating pain and suppressing cartilage degeneration, osteophyte formation, and synovial inflammation. The autologous origin of extracellular vesicles and scalable purification method make our strategy potentially viable for clinical translation.CLINICAL RELEVANCE: Infrapatellar fat pad-derived MSC-extracellular vesicles isolated by anion exchange chromatography can suppress OA progression in a mouse model. Further studies with large-animal models, larger animal groups, and subsequen...
Source: Clinical Orthopaedics and Related Research - April 25, 2024 Category: Orthopaedics Authors: Qisong Liu Jianqun Wu Hua Wang Zhaofeng Jia Guangheng Li Source Type: research
Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model
In this study, we prepared exosomes (EXOs) from cartilage stem/progenitor cells (CSPCs) and found that treatment with EXOs increased the viability, migration, and proliferation of cultured primary chondrocytes. In a subacute OA rat model, the application of EXOs facilitated cartilage regeneration as evidenced by histological staining. Exosomal protein analysis together with bioinformatics suggested that cyclin-dependent kinase 9 (CDK9) is a key factor for chondrocyte growth and migration. Functional studies confirmed this prediction, that is, inhibiting CDK9 reduced the beneficial effects induced by EXOs in primary chondro...
Source: J Cell Mol Med - April 25, 2024 Category: Molecular Biology Authors: Jing Chen Xiaohui Ni Jian Yang Hongwei Yang Xiaoyu Liu Minhao Chen Cheng Sun Youhua Wang Source Type: research
CHMP5 attenuates osteoarthritis via inhibiting chondrocyte apoptosis and extracellular matrix degradation: involvement of NF- κB pathway
CONCLUSIONS: This study demonstrated that CHMP5 repressed IL-1β-induced chondrocyte apoptosis and ECM degradation and blocked NF-κB activation. It was shown that CHMP5 might be a novel potential therapeutic target for OA in the future.PMID:38664616 | PMC:PMC11046779 | DOI:10.1186/s10020-024-00819-6 (Source: Molecular Medicine)
Source: Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Weilu Gao Rui Liu Keke Huang Wenhan Fu Anquan Wang Gongwen Du Hao Tang Li Yin Zongsheng S Yin Source Type: research
Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model
In this study, we prepared exosomes (EXOs) from cartilage stem/progenitor cells (CSPCs) and found that treatment with EXOs increased the viability, migration, and proliferation of cultured primary chondrocytes. In a subacute OA rat model, the application of EXOs facilitated cartilage regeneration as evidenced by histological staining. Exosomal protein analysis together with bioinformatics suggested that cyclin-dependent kinase 9 (CDK9) is a key factor for chondrocyte growth and migration. Functional studies confirmed this prediction, that is, inhibiting CDK9 reduced the beneficial effects induced by EXOs in primary chondro...
Source: Molecular Medicine - April 25, 2024 Category: Molecular Biology Authors: Jing Chen Xiaohui Ni Jian Yang Hongwei Yang Xiaoyu Liu Minhao Chen Cheng Sun Youhua Wang Source Type: research
Control of articular degeneration by extracellular vesicles from stem/stromal cells as a potential strategy for the treatment of osteoarthritis
Biochem Pharmacol. 2024 Apr 23:116226. doi: 10.1016/j.bcp.2024.116226. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a degenerative joint condition that contributes to years lived with disability. Current therapeutic approaches are limited as there are no disease-modifying interventions able to delay or inhibit the progression of disease. In recent years there has been an increasing interest in the immunomodulatory and regenerative properties of mesenchymal stem/stromal cells (MSCs) to develop new OA therapies. Extracellular vesicles (EVs) mediate many of the biological effects of these cells and may represent an al...
Source: Biochemical Pharmacology - April 25, 2024 Category: Drugs & Pharmacology Authors: Mar ía José Alcaraz Source Type: research
