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Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus
AbstractA new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5 ′ and 3′ untranslated regions (UTRs), followed by a poly(A) tail at the 3′ end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organ izati...
Source: Archives of Virology - February 11, 2023 Category: Virology Source Type: research

Molecules, Vol. 28, Pages 1505: Intraspecific Variability of Stinging Nettle (Urtica dioica L.)
ączek The purpose of the work was to determine the intraspecific variability of the stinging nettle, in respect of the mass of leaves and their chemical composition, including the content of phenolic compounds and assimilative pigments. The objects of the study were 10 populations of nettle, originating from the eastern and southern part of Poland. The results obtained indicate a high level of variability between and within the populations investigated but not strictly related to their geographical locations. The mass of the leaves ranged from 0.19 to 0.28 kg dry weight (DW)/plant (Coefficient of variation (CV) = 16.3...
Source: Molecules - February 3, 2023 Category: Chemistry Authors: Sylwia Koczkodaj Jaros ław L. Przybył Olga Kosakowska Zenon W ęglarz Katarzyna B. B ączek Tags: Article Source Type: research

Mobilisation of Cd, Mn, and Zn in floodplains by action of plants and its consequences for spreading historical contamination and fluvial geochemistry
Environ Sci Pollut Res Int. 2023 Jan 7. doi: 10.1007/s11356-022-25113-y. Online ahead of print.ABSTRACTCadmium, Mn, and Zn are mobilised by plants commonly growing in floodplains, most notably willows (Salix) and alder (Alnus). These plants accumulate unwanted elements (Cd) or excessive element concentrations (Mn, Zn) in their foliage, thus introducing them into the food web and enriching them in floodplain surface by litterfall. In floodplain of the Litavka River in Czechia, contaminated by historical mining activities, up to 100 mg kg-1 Cd and up to several thousand mg kg-1 Mn and Zn are present in willow leaves in autum...
Source: Environmental Science and Pollution Research International - January 7, 2023 Category: Environmental Health Authors: Tom áš Matys Grygar Michal Ho šek Jitka Elznicov á Iva Machov á Karel Kub át Slavom ír Adamec Štěpánka Tůmová Jan Rohovec Tom áš Navrátil Source Type: research

Molecules, Vol. 27, Pages 8872: Hydroethanolic Extract of Urtica dioica L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer
Jaradat Herbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of Urtica dioica (UD) hydroethanolic extract and to screen its antidiabetic activity by disaccharidase hindering and glucose transport in Caco-2 cells. The results have shown that a total of 13 phenolic compounds in this work, viz. caffeic and coumaric acid esters (1, 2, 4&ndash;7, 10), ferulic derivative (3), and flavonoid glycosides (8, 9, 11&ndash;13), were identified us...
Source: Molecules - December 14, 2022 Category: Chemistry Authors: Mohammad A. Altamimi Ibrahim M. Abu-Reidah Almothana Altamimi Nidal Jaradat Tags: Article Source Type: research

Molecules, Vol. 27, Pages 8629: Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
Singh Vamanu Singh Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high c...
Source: Molecules - December 6, 2022 Category: Chemistry Authors: Yousuf Shabir Kauts Minocha Obaid Khan Mujalli Jamous Almaghrabi Baothman Hjazi Singh Vamanu Singh Tags: Article Source Type: research

Complementary and Alternative Medicines Used by Individuals With Rheumatological Diseases to Cope With Sleeplessness
This study aims to determine complementary and alternative medicine methods used by rheumatology patients to cope with insomnia and to evaluate their attitudes toward complementary and alternative medicine methods. This descriptive study was completed with 167 patients followed up by the rheumatology unit of a university hospital. Data were collected with the patient identification form and the Holistic Complementary and Alternative Medicine Questionnaire. The mean age of the participants was 44.76 ± 8.96 years and the mean disease duration was 52.90 ± 35.51 months. Most of the participants (69.5%) were women, with 64.1%...
Source: Holistic Nursing Practice - November 23, 2022 Category: Nursing Tags: FEATURES Source Type: research

Elevated Urbanization-Driven Plant Accumulation of Metal(loid)s Including Arsenic Species and Assessment of the K łodnica River Sediment Contamination
Arch Environ Contam Toxicol. 2022 Nov 16. doi: 10.1007/s00244-022-00967-y. Online ahead of print.ABSTRACTThe impact of water and bottom sediment pollution of a river subjected to a strong industrial anthropogenic pressure of metal(loid) (including arsenic and its species) accumulation in riverbank plants such as Solidago virgaurea L., Phragmites L. and Urtica dioica L. was investigated. The high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) technique was used to study organic and inorganic arsenic species in selected plants and their response to heavy metal and arsenic contami...
Source: Archives of Environmental Contamination and Toxicology - November 17, 2022 Category: Environmental Health Authors: Magdalena Jab łońska-Czapla Katarzyna Grygoy ć Source Type: research

Viruses, Vol. 14, Pages 2511: Weed Hosts Represent an Important Reservoir of Turnip Yellows Virus and a Possible Source of Virus Introduction into Oilseed Rape Crop
umar Kundu Turnip yellows virus (TuYV) is one of the most important pathogens of oilseed rape worldwide. The virus has a large host range including many crop species (e.g., oilseed rape, pea, chickpea) and weeds from more than twenty plant families. Other than oilseed rape, we detected TuYV in many commonly grown weed species that share the fields and vegetation period together with canola crops in Czech and Slovak Republics. TuYV was detected by reverse-transcription polymerase chain reaction (RT-PCR) in at least 26 species including main crop hosts (oilseed rape), intercrops and weeds such as Amaranthus retroflexus, ...
Source: Viruses - November 13, 2022 Category: Virology Authors: Lucie Slav íková Emad Ibrahim Glenda Alquicer Jana Toma šechová Katar ína Šoltys Miroslav Glasa Jiban Kumar Kundu Tags: Communication Source Type: research

Drug-Induced Liver Injury from Herbal Liver Detoxification Tea
We describe the case of a 36-year-old female who developed a clinically significant acute liver injury with a cholestatic pattern due to an over-the-counter herbal liver detox tea. This is the first case reported of a hepatotoxic effect from any of these compounds or ingredients in the detox tea: burdock root, stinging nettle leaf, cleavers herb, dandelion root, lemon peel, and lemon myrtle leaf (Backhousia citriodora). Idiosyncratic drug-induced liver injury (DILI) remains poorly understood; however, recognizing potential toxins is imperative to understanding toxicogenomics and identifying those at risk.Case Rep Gastroent...
Source: Case Reports in Gastroenterology - November 8, 2022 Category: Gastroenterology Source Type: research

Molecules, Vol. 27, Pages 7557: Simultaneous Removal of Polymers with Different Ionic Character from Their Mixed Solutions Using Herb-Based Biochars and Activated Carbons
cki Nettle and the sage herbs were used to obtain carbonaceous adsorbents. For the biochar preparation the precursors were dried and subjected to conventional pyrolysis. Activated carbons were obtained during precursor impregnation with phosphoric(V) acid and multistep pyrolysis. The textural parameters and acidic-basic properties of the obtained adsorbents were studied. The activated carbons prepared from the above herbs were characterized by the largely developed specific surface area. The obtained carbonaceous adsorbents were used for polymer removal from aqueous solution. Poly(acrylic acid) (PAA) and polyethylenimi...
Source: Molecules - November 4, 2022 Category: Chemistry Authors: Marlena G ęca Ma łgorzata Wiśniewska Piotr Nowicki Tags: Article Source Type: research

Increased Focus on Internal Body Sensations Is Associated with Higher Patient-Perceived Severity of Allergic Rhinitis
Conclusions: Our data suggest that patients who are more aware of internal stimuli, as well as those who are highly self-conscious and somatically concerned tend to experience more severe AR symptoms.Int Arch Allergy Immunol (Source: International Archives of Allergy and Immunology)
Source: International Archives of Allergy and Immunology - November 3, 2022 Category: Allergy & Immunology Source Type: research

Effects of < em > Urtica dioica < /em > agglutinin on glycotargeting of the vasculature: an < em > in ovo < /em > study on chicken embryo
In this study we investigated effect of Urtica dioica agglutinin (UDA), as an unusual phyto-lectin from the chitin-binding protein family, on the angiogenesis of chicken embryos. The UDA was extracted from plant rhizomes and purified by affinity chromatography column. The activity of this lectin was assayed by hemagglutination test on the human RBCs. Anti-angiogenic effect of UDA on the extra-embryonic layer of the chick egg was studied in the different concentrations. Our results showed that the minimum concentration of UDA for agglutination were 48.00 and 15.00 µg mL-1 in macro- and microscopic studies, respectively. Be...
Source: Cell Research - November 2, 2022 Category: Cytology Authors: Esmaeil Samadian Abasalt Hosseinzadeh Colagar Jahanbakhsh Asadi Source Type: research

Summer pollen flora in rural and urban central England dominated by nettle, ryegrass and other pollen missed by the national aerobiological network
AbstractAbundance and diversity of airborne pollen are important to human health and biodiversity. The UK operational network collects airborne pollen from 8 flowering trees, grasses and three weeds using Hirst traps and microscopic identification from urban areas. Knowledge of total pollen diversity and differences between rural and urban zones is limited. We collect environmental DNA (eDNA) from air during summer and autumn over 3 years with mini cyclones from one urban and one rural site. Data are analysed using next generation sequencing and metabarcoding. We find the most common genus,Urtica (57%), is also identifie...
Source: Aerobiologia - October 11, 2022 Category: Environmental Health Source Type: research