Prevalence of airborne fungal spores in two potato warehouses with different storage conditions
This study constitutes an approach to detecting fungal spores present inside potato warehouses and the influence of the environmental conditions on the prevalence of the different types of fungi. The most common spores wereCladosporium andAspergillus/Penicillium representing more than 94% of the identified spores of fungi. However, some potential fungal potato pathogens such asAlternaria,Helminthosporium,Fusarium andPhytophthora were also detected. In the warehouse without control of the storage conditions, the most represented fungal spores were fromCladosporium followed byAspergillus/Penicillium, Helminthosporium andAlte...
Source: Aerobiologia - February 6, 2021 Category: Environmental Health Source Type: research

Microbial community structure in PM 2.5 affected by burning fireworks during the 2019 Spring Festival in Guilin, a touristic city in South China
In this study, PM2.5 samples were collected at four different sites in the city of Guilin during Spring Festival from February 4 to 8, 2019 and a high-throughput sequencing technology was used to investigate the variations of bacterial diversity and community structure in PM2.5. The results revealed thatProteobacteria was the predominant bacterial phylum comprising 94.3% of the total sequences, followed byFirmicutes,Actinobacteria,Bacteroidetes, andDeinococcus-Thermus. At the genus level, the top five abundant genera accounted for 81.8% of the total sequences. The proportions of major genera (Cupriavidus, Sphingobium, Brev...
Source: Aerobiologia - February 6, 2021 Category: Environmental Health Source Type: research

Bioaerosols in the landfill environment: an overview of microbial diversity and potential health hazards
AbstractLandfilling is one of the indispensable parts of solid waste management in various countries. Solid waste disposed of in landfill sites provides nutrients for the proliferation of pathogenic microbes which are aerosolized into the atmosphere due to the local meteorology and various waste disposal activities. Bioaerosols released from landfill sites can create health issues for employees and adjoining public. The present study offers an overview of the microbial diversity reported in the air samples collected from various landfill sites  worldwide. This paper also discusses other aspects, including effect of&nb...
Source: Aerobiologia - February 4, 2021 Category: Environmental Health Source Type: research

Stochastic flowering phenology in Dactylis glomerata populations described by Markov chain modelling
AbstractUnderstanding the relationship between flowering patterns and pollen dispersal is important in climate change modelling, pollen forecasting, forestry and agriculture. Enhanced understanding of this connection can be gained through detailed spatial and temporal flowering observations on a population level, combined with modelling simulating the dynamics. Species with large distribution ranges, long flowering seasons, high pollen production and naturally large populations can be used to illustrate these dynamics. Revealing and simulating species-specific demographic and stochastic elements in the flowering process wi...
Source: Aerobiologia - February 3, 2021 Category: Environmental Health Source Type: research

Airborne pollen and spores ’ deposition in alveolar tissues as a tool in drowning forensic diagnosis
We report the results of a histological study of lung samples where an unusual quantity of airborne pollen and fungal spores was found in drowned rats. Pollen and spores were found in lungs of drowned rats but not in the post-mortem submerged ones. Another control group consisting of rats that underwent 60  min exposure to a highly pollen-loaded atmosphere also recorded negative for the presence of pollen or fungal spores. Pollen types coincided with plants growing at the surrounding gardens flowering during the days of the experiment, performed during spring, that were detected by the aerobiological trap located at t...
Source: Aerobiologia - January 22, 2021 Category: Environmental Health Source Type: research

Germination dynamics of allergenic fungal spores in respiratory mucus
This study aimed to investigate the temporal germination dynamics of allergenicCladosporium, Aspergillus, andPenicillium spp. in respiratory mucus ex situ and the effect of the temperature (28  °C and 37 °C in the upper and lower respiratory tract, respectively) on fungal germination. Fungal spore germination was inhibited at 37 °C inC. oxysporum andC. cladosporioides, but notA. flavus,A. brunneoviolaceus,A. protuberus,P. citrinum, andP. oxalicum. Aspergillus flavus andA. brunneoviolaceus exhibited a high germination rate in the mucus at 28  °C and 37 °C. This indicated that the...
Source: Aerobiologia - January 20, 2021 Category: Environmental Health Source Type: research

Quantitative and qualitative assessment of microbial aerosols in different indoor environments of a dental school clinic
AbstractIn the indoor environment of dental clinics, dental staff and patients are exposed to various types of infectious agents transported by aerosols and particles, generated during dental procedures, promoting an increased risk of cross-infection. The aim of this study was to determine the levels and diversity of microbial aerosol in relation to particle load in five different departments of a dental school clinic. The air samples were collected by an active single-stage Andersen sampler during the treatment procedure. The mean concentrations of airborne bacteria were in the range of 52 –1030 and 8–844 ...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research

Effect of selected sampling media, flow rate, and time on the sampling efficiency of a liquid impinger packed with glass beads for the collection of airborne viruses
AbstractThe liquid impingers can be used for sampling of viral aerosols, such as COVID-19 virus, influenza, and measles. However, the lowest cutoff diameter of commercially available liquid impingers was about 0.3  μm, and the physical collection efficiency for nano-bioaerosol is only about 10–20%. Here, we enhanced the impinger’s collection efficiency and recovery of viable viral aerosols by using packed glass beads and selected sampling media (1% peptone and lysogeny broth, LB). Single-stranded RNA (s sRNA) MS2 bacteriophage with uranine (as a physical tracer) was used as model viral aerosols. The eff...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research

Morning –evening variation and determinants of bacterial and fungal aerosol concentration in historical objects in Kraków
AbstractCultural heritage objects are very frequently visited by tourists, the presence of which is considered to affect the bacterial and fungal aerosol levels in these facilities. Therefore, this study had two main aims with the first to determine the morning/evening changes in the concentration of bacterial and fungal aerosol as well as particulate matter in the selected historical objects of Krak ów: churches, crypts, libraries, and museums. The second aim was to establish the most possible factors affecting the quality of air in the examined sites using data mining techniques: principal component analysis (PCA)...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research

Properties relevant to atmospheric dispersal of the ice-nucleation active Pseudomonas syringae strain R10.79 isolated from rain water
AbstractTransport of microbes in the atmosphere allows them to spread and to colonize new habitats. To survive the harsh environmental conditions encountered in the atmosphere, these microorganisms have to possess properties that allow them to resist atmospheric stress. We combined physiological experiments and genome analysis ofPseudomonas syringae strain R10.79, previously isolated from rain, to decipher the strain ’s ability to withstand these challenges and identify the mechanisms behind the resistance. We used laboratory experiments to simulate different atmospheric stress factors and evaluated cell survival usi...
Source: Aerobiologia - January 13, 2021 Category: Environmental Health Source Type: research

Pollen Ole e 1 content variations in olive cultivars of different Portugal regions
AbstractOlive is recognized as a crop with great impact in agricultural, socioeconomic, environmental and public health sectors. The last is becoming more important during recent years as consequence of the increase of the pollen allergy in south Europe prompted by the widespreadOlea pollen allergic reactions. The aim of the study was to quantify, for the first time, the variations of the Ole e 1 allergen amount inOlea pollen grains from four cultivars in three regions of Portugal. How weather parameters can affect the allergen production was also assessed. The study was conducted in three olive producer areas of Portugal ...
Source: Aerobiologia - January 12, 2021 Category: Environmental Health Source Type: research

Efficacy of 75% alcohol in pretreatment of the Andersen sampler in trapping maximum airborne microbes
AbstractThe present study was conducted to evaluate the effects of the pretreatment methods and sampling time on the sampling of airborne bacteria in hospitals. Methods for the pretreatment of Andersen samplers, namely, non-sterilized, 75% ethanol and autoclaving sampled for 5  min, 10 min and 15 min in the general ward and class 1000 clean operating department, respectively, were studied. Statistical analysis was used to compare the differences in sampling results of airborne bacteria under different pretreatment methods, sampling time and environmental conditions. In the first test, the sampling results of...
Source: Aerobiologia - January 5, 2021 Category: Environmental Health Source Type: research

Correction to: Bioaerosol measurements over a fattening period in a pig barn focused on the presence of Staphylococcus spp.
In the original publication of the article, the below errors have occurred. (Source: Aerobiologia)
Source: Aerobiologia - January 3, 2021 Category: Environmental Health Source Type: research

Spatial distribution of atmospheric bioaerosols in Beijing, Hangzhou and Wuhan, China
In this study, air samples were collected in various planned areas of three cities in China (Beijing, Hangzhou and Wuhan) using a new liquid-based sampler. Animal, plant and bacterial components were quantified according to the expression levels of housekeeping genes via real-time PCR and analysis of cycle threshold (Ct) values. The level of animal-derived particles in the air of Hangzhou was higher than those in the other two cities. More bacterial particles than animal or plant particles were detected in the air in Beijing compared with other cities. We also found a negative correlation between the bacterial content of b...
Source: Aerobiologia - January 2, 2021 Category: Environmental Health Source Type: research

Assessment of indoor air quality in geriatric environments of southwestern Europe
The objective of this study was to evaluate physical –chemical and microbial indicators of indoor air quality in three nursing houses (NHs) located in rural areas of the northeast of Portugal. The parameters were measured during two campaigns (winter and summer), twice a day, and in four distinct spaces for spatial variability assessment: dining roo m, living room, double bedroom and outdoor of the NHs. Physical–chemical indicators were assessed by using a Graywolf IQ 610 probe. Airborne microbial levels were evaluated by direct impaction to the culture media, and subsequently, the microorganisms were identifie...
Source: Aerobiologia - January 1, 2021 Category: Environmental Health Source Type: research

Air pollutants NO 2 - and O 3 -induced Dactylis glomerata L. pollen oxidative defences and enhanced its allergenic potential
AbstractAir pollutants impact airborne pollen biochemistry. Oxidative damage to lipids, proteins and nucleic acids or protein nitration are among ozone (O3) and nitrogen dioxide (NO2) described deleterious effects possibly causing pollen physiology damage and enhanced allergenic activity, contributing to aggravate pollen driven respiratory allergy in urban areas. The goal of this research was to evaluate the effects of O3, NO2, alone and combined, onDactylis glomerata pollen reactive oxygen species scavenging enzymes, on pollen germination and their potential contribution to the allergenicity.D. glomerata pollen was in vit...
Source: Aerobiologia - November 27, 2020 Category: Environmental Health Source Type: research

Antifungal effects of Curcuma longa L. essential oil against pathogenic strains isolated from indoor air
The objective of this work was to evaluate the antifungal activity ofC. longa essential oil on potentially pathogenic and biodegradable strains isolated from the indoor of the food industry. A suspension of 106 spores/mL of the strainsAspergillus flavus,Penicillium brevicompactum andCladosporium cladosporioides was prepared. Two natural biocides (C. longa essential oil and Cuban propolis) and two synthetic ones (antiseptic detergent and Tween 20) at different concentrations (0, 0.001, 0.1 and 1.0% v/v) were evaluated. The superficial growth of the mycelium (cm) and the germination inhibition (%) were the response variables...
Source: Aerobiologia - November 22, 2020 Category: Environmental Health Source Type: research

Morphophysiological characteristics of pollen grains produced by bisexual inflorescences of silver birch ( Betula pendula Roth.)
(Source: Aerobiologia)
Source: Aerobiologia - November 21, 2020 Category: Environmental Health Source Type: research

Spatiotemporal variation of ambient bioaerosols in a large and industrialized metropolis of Iran and their association with PM 2.5 and meteorological factors
The objective of this study was to investigate the levels of airborne bacteria and fungi and their association with PM2.5 and meteorological parameters in Isfahan. Air samples were collected from seven different locations of Isfahan city using SKC pumps connected to glass impingers containing a buffer solution and analyzed by the cell culture method. The meteorological variables were obtained from the provincial meteorological office, and the data of atmospheric PM2.5 were taken from the Department of Environment. The highest and the lowest bacterial levels were found at station 7 (114.4 (40 –254.2) CFU/m3) and ...
Source: Aerobiologia - November 19, 2020 Category: Environmental Health Source Type: research

COVID-19 lockdown: a boon in boosting the air quality of major Indian Metropolitan Cities
AbstractThe COVID-19 lockdown has not only helped in combating the community transmission of SARS-CoV-2 but also improved air quality in a very emphatic manner in most of the countries. In India, the first phase of COVID-19 lockdown came into force on March 25, 2020, which was later continued in the next phases. The purpose of this study was to investigate the result of lockdown on air quality of major metropolitan cities —Delhi, Mumbai, Kolkata, Chennai, Bengaluru, Hyderabad, Jaipur, and Lucknow—from March 25 to May 3, 2020. For this study, the concentration of six criteria air pollutants (PM2.5, PM10, CO, NO2...
Source: Aerobiologia - November 17, 2020 Category: Environmental Health Source Type: research

A preliminary investigation on airborne fungi of pedestrian underpasses
AbstractPedestrian underpasses are the unique environment where air transaction with outdoor is complicated, inducing interest to study the air quality for the presence of microbes. Thus, the study on the presence of airborne fungi from 11 different underpasses in Chennai city was conducted. The airborne fungi was monitored by exposing potato dextrose agar strips in reuter centrifugal sampler (RCS). A total of 1735 colonies were isolated from underpasses and the control site recorded only 1255 colonies. Total average colony forming unit of underpasses and control site was 157.7  CFU/m3 of air and 114.09  CFU/m3 o...
Source: Aerobiologia - November 16, 2020 Category: Environmental Health Source Type: research

Assessment of airborne Curvularia propagules in the atmosphere of Havana, Cuba
AbstractEven though the presence ofCurvularia conidia in the tropical air and the role in respiratory allergy symptoms or plant diseases incidence has been reported, traditional aerobiological investigations usually do not consider their profound study. An aeromycological study of theCurvularia propagules in Havana was conducted during the years 2017 and 2018. Monitoring was carried out by means of volumetric viable and non-viable methodologies. Three types of microscopically identifiable airborne spores (Lunata, Geniculata and Maculans) were identified, according to their distinctive features (conidium shape, pigmentation...
Source: Aerobiologia - November 16, 2020 Category: Environmental Health Source Type: research

Allergenic fungal spores in the air of urban parks
AbstractUrban green spaces, especially urban parks, are essential for the proper functioning of cities, but they can be a serious source of airborne fungal spores. Aerobiological monitoring was carried out in urban parks of different typology to estimate the risk associated with fungal spores for citizens. Volumetric method was applied with the use of portable Burkard Sampler. In the air of the studied parks, the most dominant spores are strong allergenic or considered as potentially allergenic.Cladosporium spores were found in enormous concentrations in all studied parks, and it affected the low biodiversity of fungal spo...
Source: Aerobiologia - November 11, 2020 Category: Environmental Health Source Type: research

Disinfection efficiency of hospital infectious disease wards with chlorine dioxide and hypochlorous acid
AbstractThe disinfection efficiencies of two chemical disinfectants, chlorine dioxide and weak acid hypochlorous water (WAHW), were examined in the soiled room and dishwashing room of a hospital infectious disease ward in Taiwan. The investigations were conducted in two seasons, namely winter and summer, in order to examine the correlation between the bioaerosol concentration and the environmental factors. In addition, a single-daily disinfection mode (SM) and a twice-daily disinfection mode (TM) were applied in this study. The results showed that the bacteria and fungi colony counts were strongly correlated with the tempe...
Source: Aerobiologia - November 5, 2020 Category: Environmental Health Source Type: research

Testing the Raman parameters of pollen spectra in automatic identification
AbstractPollen identification and quantification are used in many fields of application and research has been conducted to attain accurate automatic pollen recognition aiming to reduce the laborious work and subjectivity in human identification. The aim of our study was to evaluate the capacity of Raman parameters of pollen spectra, calculated for only 7 common band intervals in a limited spectral range, to be used as future technique in pollen automatic identification. There were analyzed 15 different pollen species considered to induce allergic reactions. Raman spectra were acquired at an excitation wavelength of 785 &nb...
Source: Aerobiologia - October 28, 2020 Category: Environmental Health Source Type: research

The EUMETNET AutoPollen programme: establishing a prototype automatic pollen monitoring network in Europe
AbstractOperational pollen monitoring networks have developed across Europe, and the world more generally, in response to the increasing prevalence of pollen allergy and asthma. Routine pollen observations are in large part currently still based on time-intensive manual techniques developed in the 1950s. These methods suffer from low temporal resolution and long delays in data availability. Recent technological developments are revolutionising the field making real-time high-temporal resolution measurements possible. This paper describes the rationale behind the EUMETNET AutoPollen programme, which aims to develop a protot...
Source: Aerobiologia - October 20, 2020 Category: Environmental Health Source Type: research

Forecasting olive ( Olea europaea L.) production using aerobiological and meteorological variables in T étouan (NW Morocco)
This study describes the first forecasting models of the olive fruit production based on pre-peak airborne annual pollen integral (APIn) fromOlea europaea L. and meteorological data prior and during the flowering and ripening olive trees in T étouan (NW of Morocco) over a period of 11 years (2008–2018). Aerobiological sampling was conducted using Burkard volumetric Hirst trap. The data were analyzed by multiple regression analysis. Several forecasting models developed were validated using data of 2018 (not included in the models) and compared with real olive crop data obtained from the Provincial Directio...
Source: Aerobiologia - October 20, 2020 Category: Environmental Health Source Type: research

Pollen calendars and regional gradients as information tools in the Extremadura pollen monitoring network (SW Spain)
AbstractPollen allergies are one of the most important problems among respiratory diseases in today ’s society. The creation and development of aerobiological information tools are useful to provide information to patients and doctors. In this work, we analysed the pollen spectrum of Extremadura to generate pollen calendars for Badajoz, Cáceres, Don Benito, Plasencia and Zafra. Additionally, we analysed regional pollen gradients and plotted the main pollen season (MPS) characteristics (start date, peak date, end date and duration) in each city. In Extremadura, 35–40 different types of pollen are frequent...
Source: Aerobiologia - October 19, 2020 Category: Environmental Health Source Type: research

Airborne fungal phytopathological spore assessment in three European vineyards from different bioclimatic areas
AbstractThe most common fungal diseases ofVitis vinifera are powdery mildew (Erysiphe necator (Schw.) Burr.), grey mould (Botrytis cinerea Pers.) and downy mildew (Plasmopara viticola (Berk. and Curt.) Berk. and de Toni). Knowledge about the concentration of their spores in the atmosphere of the vineyard is important in order to ascertain the fungal load in the vineyard during the reproductive vine cycle. An aerobiological study was conducted in three vineyards across Europe: in North-Western Spain (Cenlle), South Spain (Montilla) and South-Eastern Poland (Rzesz ów). During 2016, three Hirst-type spore traps sampled...
Source: Aerobiologia - October 12, 2020 Category: Environmental Health Source Type: research

Influence of Phleum pratense pollen grains rupture on lipids extraction
AbstractIn this work, we completed the first identification and quantification of internal lipids for allergenicPhleum pratense pollen grains (PGs). We have extracted PGs with aqueous solvent under different extraction procedures and observed release of extra amount of unsaturated fatty acids (α-linolenic and linoleic acids) when PGs were broken during extraction. We showed that rupture of PGs is necessary for the release of higher quantity of bioactive compounds. Organic solvent extraction of crushed PGs confirmed the predominance of unsaturated fatty acids in the inner parts of PGs as well as a different distributi...
Source: Aerobiologia - October 6, 2020 Category: Environmental Health Source Type: research

The impact of weather conditions on hazel pollen concentration in Sosnowiec (Poland) in 1997 –2019
AbstractThe goal of this study was to compare hazel pollen seasons in Sosnowiec in 1997 –2019 and to analyse the impact of weather conditions on these seasons. The measurements were conducted using a volumetric method with a Burkard spore trap. The duration of pollen seasons was determined using the 98% method. SPI (Seasonal Pollen Index) was calculated as the sum of daily pollen con centrations in a given season. The measurements showed that high temperatures in January and February had an impact on the beginning of the hazel pollen season. They revealed that there are positive correlations with temperatures and sun...
Source: Aerobiologia - October 6, 2020 Category: Environmental Health Source Type: research

The late flowering of invasive species contributes to the increase of Artemisia allergenic pollen in autumn: an analysis of 25  years of aerobiological data (1995–2019) in Trentino-Alto Adige (Northern Italy)
AbstractArtemisia pollen is an important aeroallergen in late summer, especially in central and eastern Europe where distinct anemophilousArtemisia spp. produce high amounts of pollen grains. The study aims at: (i) analyzing the temporal pattern of and changes in theArtemisia spp. pollen season; (ii) identifying theArtemisia species responsible for the local airborne pollen load.Daily pollen concentration ofArtemisia spp. was analyzed at two sites (BZ and SM) in Trentino-Alto Adige, North Italy, from 1995 to 2019.The analysis of airborneArtemisia pollen concentrations evidences the presence of a bimodal curve, with two pea...
Source: Aerobiologia - October 1, 2020 Category: Environmental Health Source Type: research

Bioaerosol measurements over a fattening period in a pig barn focused on the presence of Staphylococcus spp.
The objective of the present study was to estimate the distribution of bioaerosols in the animal husbandry and its surroundings. Over 2 fattening periods with different animal ages and weights, the main emphasis was put on the total concentrations of mesophilic bacteria andStaphylococcus spp., especially the speciesS. aureus. The bioaerosols were sucked in with an AGI-30 Impinger, and nasal and neck skinfold swab samples were collected supplementary from randomly selected pigs. From the first series of measurements, the total concentration of mesophilic bacteria was 6.2  × 105 cfu/m3 and dropped to...
Source: Aerobiologia - September 24, 2020 Category: Environmental Health Source Type: research

Evaluation of hirst-type sampler and PM 10 impactor for investigating adhesion of atmospheric particles onto allergenic pollen grains
AbstractHirst-type sampler (HTS) is the standard equipment  for pollen sampling while cascade impactors are commonly used for particulate matter (PM) samplings. In this work, we investigated the feasibility of using such devices to reliably assess the atmospheric PM adhesion onto pollen grains (PGs). Birch PGs were deposited on sampling substrates and the n exposed to ambient PM in each type of sampling device operating simultaneously. In the HTS, the surface of PGs was significantly polluted with PM during sampling (93.5% of polluted PGs with on average 4 particles per PG with a mean diameter of 2.2 &m...
Source: Aerobiologia - September 24, 2020 Category: Environmental Health Source Type: research

First phenological and aerobiological assessment of olive orchards at the Northern limit of the Mediterranean bioclimatic area
AbstractAn increase in the cultivation of olive trees was prompted in the Northwestern Spain during recent years favored by the agricultural politics in the region. The aim of this study is to know the phenological and aerobiological behavior of the olive trees at the northern limit of the Mediterranean bioclimatic area in order to determine their thermal requirements for the development of predictive agro-phenological models for two varieties (Arbequina and Frantoio) of the new olive cultivated areas. The BBCH scale was used for the phenological observations. Chill accumulation during dormancy and heat requirements to ove...
Source: Aerobiologia - September 24, 2020 Category: Environmental Health Source Type: research

Proposal for monitoring approach and control of air mycobiota in the Grand Egyptian Museum —Conservation Center
This study aimed to monitor and assess the biodiversity and concentration of airborne fungi for all areas of the Grand Egyptian Museum —Conservation Center (GEM-CC), such as conservation laboratories, scientific laboratories, corridors, storerooms, and outside in each season during 2013 using the air sampler machine. Results of the quantitative analysis of fungal aerosol demonstrated that the air quality level in GEM-CC was gener ally acceptable, though some areas showed deviation from the threshold limit value of the GEM-CC (200 CFU m−3). Also, the fungal load in all storerooms was lower than inside ...
Source: Aerobiologia - September 22, 2020 Category: Environmental Health Source Type: research

Aerosol microbial community structure analysis based on two different sequencing platforms
In this study, air microbial samples from two sampling points in the coastal city of Qingdao were collected by a large volume air sampler. The Illumina MiSeq and PacBio high-throughput sequencing methods were used to analyze the microbial community structure and diversity of the two sampling points. The results showed that there were significant differences in the number and relative abundances of microorganisms identified by the two platforms, and with the refinement of the sample classification, the differences in the community became increasingly significant. Proteobacteria and Firmicutes were the dominant bacterial phy...
Source: Aerobiologia - September 20, 2020 Category: Environmental Health Source Type: research

Standardising pollen monitoring: quantifying confidence intervals for measurements of airborne pollen concentration
AbstractMeasurement of atmospheric pollen concentrations is inexact, yet pollen concentrations are universally reported without estimate of accuracy. These imprecise values are nevertheless used for modelling, forecasting and public health decision-making. Estimation of the variability in reported pollen concentrations would help resolve associations between weather and pollen aerobiology as well as associations between pollen exposure and health. For any given daily atmospheric pollen level, a statistical variability would be expected in the set of possible measures. This variability is introduced and compounded by many f...
Source: Aerobiologia - September 15, 2020 Category: Environmental Health Source Type: research

Biodiversity of Fungi in  the photic and aphotic zones of Montenegro caves
AbstractFor the first time, the composition of micromycetes communities was investigated in both photic and aphotic entrance zones of seven caves in Montenegro: Obodska Pe ćina, Golubinja Pećina, Veluštica Pećina, Vrbacka jama, Jama ER-1, Njegoš Pećina, and an unnamed cave. In total, 72 species of micromycetes were identified: 46 species from illuminated photic zones of the caves, including 43 species on phototrophs, 10 species from the air, 15 species on subst rates, and 50 species from unlit aphotic zones, including 35 species from the air and 37 species on substrates. The most frequently isolated ...
Source: Aerobiologia - August 12, 2020 Category: Environmental Health Source Type: research

Airborne fungal spore concentration in an industrial township: distribution and relation with meteorological parameters
This study aims to perform the qualitative assessment of ambient air of Barrackpore, an industrial township of West Bengal, India, by analysing the airborne fungal spore diversity for two consecutive years. The spores of ambient air were trapped using Burkard 7-day volumetric sampler from June 2014 to May 2016. The association of major fungal taxa with environmental parameters was analysed by Spearman ’s rank correlation coefficient and multiple regression analysis to identify the significant predictors. The daily average ambient fungal spore concentration was 3526.38 ± 2709.32 spores m...
Source: Aerobiologia - July 25, 2020 Category: Environmental Health Source Type: research

Sensitization to aeroallergens in an atopic population of Algiers (Algeria)
AbstractAeroallergen sensitization is increasing worldwide. The optimal management of allergic disease requires the identification of the allergic sensitivities of the patient. The aim of the present study was to evaluate the profile of specific IgE reactivity of patients with clinically suspected allergic diseases seeking medical care in Algiers. The prevalence of 29 aeroallergens ’ sensitization was investigated by using in vitro testAllergy screen, for 1461 patients diagnostics allergic atMustapha Pasha Hospital in Algiers (Algeria), from  the period between January 2016 and  June 2019. Patients wer...
Source: Aerobiologia - July 20, 2020 Category: Environmental Health Source Type: research

Use of radiofrequency electromagnetic radiation to remove biofilms from canvasses
AbstractA method based on the use of electromagnetic radiation (EMR) in the radiofrequency band was experimented to eliminate heterotrophic organisms from biofilms encrusting linen canvasses used for paintings. Artificial biofilms were used for exposure to EMR. Biofilms were grown on fragments of canvasses sampled from the backside of a painting. The fragments, after sterilization, were inoculated with several heterotrophic microorganisms previously isolated from other canvasses used for paintings and identified by molecular analyses as the bacteriaBacillus mycoides andBacillus subtilis, and the microfungiAspergillus versi...
Source: Aerobiologia - July 9, 2020 Category: Environmental Health Source Type: research

Microbiological air quality in pharmacies and an antibiotic resistance profile of staphylococci species
AbstractThe air in public facilities contains microorganisms which may cause a wide variety of clinical syndromes in humans. This is particularly important in primary healthcare units, where patients with reduced immunity and staff are directly exposed to microbial pathogens. Air samples were collected from dispensing areas (customer areas) of five pharmacies using MAS-100 air sampler. The concentration of particular groups of microorganisms was determined with the use of selective media. The taxonomic analysis of mannitol-positive bacteria was conducted using the BIOLOG® method, based on metabolic characteristics of a...
Source: Aerobiologia - July 9, 2020 Category: Environmental Health Source Type: research

Meteorological factors driving airborne grass pollen concentration in central Iberian Peninsula
This study was carried out in the surroundings of the city of Toledo (Spain), a Mediterranean city l ocated in the center of the Iberian Peninsula. Temperature was the variable most influencing flowering onset, which was also affected by relative humidity, rainfall and hours of sunlight. The flowering period of grass species that flower at the start of spring (Bromus rubens andHordeum leporinum) began earlier in years with higher mean winter temperatures, while the species that flower from mid to late spring (Trisetaria panicea andDactylis glomerata subsp.hispanica), the flowering period began earlier in years with higher ...
Source: Aerobiologia - June 25, 2020 Category: Environmental Health Source Type: research

Microbial characteristics of culturable fungi and bacteria in aerosol particles of a coastal region
In this study, atmospheric bioaerosol particles were collected from March to December 2015 in the coastal region of Qingdao. The dominant fungi were identified, and the concentrations and size distributions of bacteria, total fungi and dominant fungi were measured. The most dominant fungal taxon wasCladosporium, followed byAlternaria, Penicillium andAspergillus. The concentrations of fungi showed significant seasonal variation (p   7.0 μm. The size patterns of the four dominant fungi were distinct due to their respective properties. With correlation analysis, we found PM10, CO and NO2 were positively ...
Source: Aerobiologia - June 23, 2020 Category: Environmental Health Source Type: research

Metagenomic characterisation of bioaerosols during the dry season in Mexico City
In this study, we employed a metagenomic approach to characterise the bacterial and fungal communities, using 16S rRNA and the internal transcribed spacer region of the nuclear ribosomal RNA. The study took place in Mexico City during the dry season, at days with high levels of ozone and suspended particles (March 14 to 18, 2016). We found a total of 147 bacterial genera, of which the most abundant ones wereMicrobispora (9%),Paracoccus (6%),Exiguobacterium (6%),Kocuria (3.0%),Friedmanniella (3%),Rubellimicrobium (2%),Sphingomonas (2%) andMethylobacterium (2%). We also found a total of 211 fungal genera, mainlyCladosporium ...
Source: Aerobiologia - June 22, 2020 Category: Environmental Health Source Type: research

Indoor biological particles in a train: comparative analysis with outdoor atmosphere
This study characterizes by using volumetric Hirst traps of the indoor pollen and fungal spore content of the indoor air of a daily crowed train connecting C órdoba city (South Spain) with the university campus. Results were compared with outdoor concentrations sampled at the same time. Twenty-three pollen types were identified indoor and 20 outdoor.Quercus, Olea, Platanus, Morus andCupressus were the most abundant. Thirteen fungal spore types were identified by both indoor and outdoor samplings. Our results pointed outCladosporium cladosporoides, Cladosporium herbarum, Coprinus, Alternaria andTorula as the most abu...
Source: Aerobiologia - June 8, 2020 Category: Environmental Health Source Type: research

Phenology of Cupressaceae urban infrastructure related to its pollen content and meteorological variables
AbstractCupressaceae pollen is one of the major airborne allergens of the Mediterranean region and in other regions around the world. Pollen production of these species causes considerable allergic problems during winter, being this type of pollen the most abundant in this period of the year. This work aims to relate phenology, meteorology and airborne pollen records in these species. Aerobiological sampling was carried out in Badajoz (SW Spain) from 2016 to 2018 using Hirst-type volumetric sampler. Pollination phenology was studied in 50 specimens, ten ofCupressus macrocarpa, ten ofCupressus arizonica, 15 ofCupressus semp...
Source: Aerobiologia - May 28, 2020 Category: Environmental Health Source Type: research

Seasonal effects in endotoxin concentration at different sections of a sewage treatment plant, Delhi
AbstractThe aim of present study is to assess the seasonal effects during five different seasons on endotoxins levels at various sections (aeration tank, sludge bed, grit chamber and final settlement tank) of a sewage treatment plant. Samples for endotoxins were collected using a three-stage cascade impactor. The sampler collects particulate matters in three different sizes, viz. PM1, PM2.5 and PM10. We mainly focused on the detection of endotoxins associated with the coarse particles (PM10-2.5) and fine particles (PM2.5-1.0). ANOVA was employed to understand whether the difference in airborne endotoxins concentrations (EU...
Source: Aerobiologia - May 16, 2020 Category: Environmental Health Source Type: research

Allergenic fungal spores and hyphal fragments in the aerosol of Havana, Cuba
The objective of this research was to analyze the fungal composition of the Havana aerosol during 2017 and 2018, emphasizing the presence of hyphae fragments. Air sampling was performed using a Lanzoni VPPS-2000 sampler. The fungal propagules collected were identified and counted following the recommendations of the Spanish Aerobiology Network in two longitudinal transepts of each daily slides. In 2017, the spore count and hyphae count were higher than in 2018 (108,607 spores and 3822 fungal fragments vs. 40,896 spores and 2268 fungal fragments).Cladosporium was the most abundant spore (77.2 –76.6%), predominating in...
Source: Aerobiologia - May 6, 2020 Category: Environmental Health Source Type: research