< em > In vitro < /em > maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes
Zygote. 2023 Nov 13:1-6. doi: 10.1017/S0967199423000473. Online ahead of print.ABSTRACTTraditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus-oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (S...
Source: Zygote - November 13, 2023 Category: Reproduction Medicine Authors: Adriano Felipe Mendes Raquel Zaneti Puelker Lilian Francisco Arantes de Souza Andr éa Renesto Coimbra Jacintho Priscila Helena Dos Santos Ines Cristina Giometti Sheila Merlo Firetti Anthony C ésar de Souza Castilho Marilice Zundt Claudia Maria Bertan Me Source Type: research
Reproductive outcomes with delayed blastocyst development: the clinical value of day 7 euploid blastocysts in frozen embryo transfer cycles
Zygote. 2023 Nov 13:1-8. doi: 10.1017/S0967199423000485. Online ahead of print.ABSTRACTEmbryos of optimal development reach blastocyst stage 116 ± 2 h after insemination. Usable D7 blastocysts represent nearly 5% of embryos in IVF with acceptable pregnancy and live birth rates, however data are still limited. Therefore, this study aimed to analyze the ongoing pregnancy rate (OPR) of D7 blastocysts in single euploid frozen embryo transfer (FET) cycles. An observational study was performed including 1527 FET cycles with blastocysts biopsied on D5 (N = 855), D6 (N = 636) and D7 (N = 36). Blastocysts were classified as good (...
Source: Zygote - November 13, 2023 Category: Reproduction Medicine Authors: Andrea Abdala Ibrahim Elkhatib A şina Bayram Ahmed El-Damen Laura Melado Daniela Nogueira Barbara Lawrenz Human M Fatemi Source Type: research
< em > In vitro < /em > maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes
Zygote. 2023 Nov 13:1-6. doi: 10.1017/S0967199423000473. Online ahead of print.ABSTRACTTraditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus-oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (S...
Source: Zygote - November 13, 2023 Category: Reproduction Medicine Authors: Adriano Felipe Mendes Raquel Zaneti Puelker Lilian Francisco Arantes de Souza Andr éa Renesto Coimbra Jacintho Priscila Helena Dos Santos Ines Cristina Giometti Sheila Merlo Firetti Anthony C ésar de Souza Castilho Marilice Zundt Claudia Maria Bertan Me Source Type: research
Reproductive outcomes with delayed blastocyst development: the clinical value of day 7 euploid blastocysts in frozen embryo transfer cycles
Zygote. 2023 Nov 13:1-8. doi: 10.1017/S0967199423000485. Online ahead of print.ABSTRACTEmbryos of optimal development reach blastocyst stage 116 ± 2 h after insemination. Usable D7 blastocysts represent nearly 5% of embryos in IVF with acceptable pregnancy and live birth rates, however data are still limited. Therefore, this study aimed to analyze the ongoing pregnancy rate (OPR) of D7 blastocysts in single euploid frozen embryo transfer (FET) cycles. An observational study was performed including 1527 FET cycles with blastocysts biopsied on D5 (N = 855), D6 (N = 636) and D7 (N = 36). Blastocysts were classified as good (...
Source: Zygote - November 13, 2023 Category: Reproduction Medicine Authors: Andrea Abdala Ibrahim Elkhatib A şina Bayram Ahmed El-Damen Laura Melado Daniela Nogueira Barbara Lawrenz Human M Fatemi Source Type: research
< em > In vitro < /em > maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes
Zygote. 2023 Nov 13:1-6. doi: 10.1017/S0967199423000473. Online ahead of print.ABSTRACTTraditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus-oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (S...
Source: Zygote - November 13, 2023 Category: Reproduction Medicine Authors: Adriano Felipe Mendes Raquel Zaneti Puelker Lilian Francisco Arantes de Souza Andr éa Renesto Coimbra Jacintho Priscila Helena Dos Santos Ines Cristina Giometti Sheila Merlo Firetti Anthony C ésar de Souza Castilho Marilice Zundt Claudia Maria Bertan Me Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
Effects of klotho protein or klotho knockdown in porcine oocytes at different stages
In this study, we injected the preassembled cas9 + sgRNA dual vector, for klotho knockdown, into the cytoplasm of the germinal vesicle stage of oocytes and into porcine embryos after 6-h parthenogenetic activation. Similarly, the klotho protein was inserted into the cytoplasm of germinal vesicle stage oocytes and porcine embryos after 6-h parthenogenetic activation. Compared with the controls, the microinjection of klotho dual vector markedly decreased the blastocyst formation rates in germinal vesicle stage oocytes and activated embryos. However, the efficiency of blastocyst formation when klotho protein was inserted befo...
Source: Zygote - October 31, 2023 Category: Reproduction Medicine Authors: Eun Pyo Kim Do Yon Kim Changhoon Park Seung-Min Yoo Myung-Shin Lee Geon A Kim Source Type: research
