Therapeutic potential of nanotechnology in reproduction disorders and possible limitations
Zygote. 2023 Aug 4:1-8. doi: 10.1017/S0967199423000424. Online ahead of print.ABSTRACTOne of the prominent peculiarities of nanoparticles (NPs) is their ability to cross biological barriers. Therefore, the development of NPs with different properties has great therapeutic potential in the area of reproduction because the association of drugs, hormones and other compounds with NPs represents an alternative for delivering substances directly at a specific site and for treatment of reproductive problems. Additionally, lipid-based NPs can be taken up by the tissues of patients with ovarian failure, deep endometriosis, testicul...
Source: Zygote - August 4, 2023 Category: Reproduction Medicine Authors: Pedro Alves Aguiar Barroso Danisv ânia Ripardo Nascimento Miguel F De Lima Neto Ernando Igo T De Assis Ciro Siqueira Figueira Jos é Roberto Viana Silva Source Type: research
Therapeutic potential of nanotechnology in reproduction disorders and possible limitations
Zygote. 2023 Aug 4:1-8. doi: 10.1017/S0967199423000424. Online ahead of print.ABSTRACTOne of the prominent peculiarities of nanoparticles (NPs) is their ability to cross biological barriers. Therefore, the development of NPs with different properties has great therapeutic potential in the area of reproduction because the association of drugs, hormones and other compounds with NPs represents an alternative for delivering substances directly at a specific site and for treatment of reproductive problems. Additionally, lipid-based NPs can be taken up by the tissues of patients with ovarian failure, deep endometriosis, testicul...
Source: Zygote - August 4, 2023 Category: Reproduction Medicine Authors: Pedro Alves Aguiar Barroso Danisv ânia Ripardo Nascimento Miguel F De Lima Neto Ernando Igo T De Assis Ciro Siqueira Figueira Jos é Roberto Viana Silva Source Type: research
Therapeutic potential of nanotechnology in reproduction disorders and possible limitations
Zygote. 2023 Aug 4:1-8. doi: 10.1017/S0967199423000424. Online ahead of print.ABSTRACTOne of the prominent peculiarities of nanoparticles (NPs) is their ability to cross biological barriers. Therefore, the development of NPs with different properties has great therapeutic potential in the area of reproduction because the association of drugs, hormones and other compounds with NPs represents an alternative for delivering substances directly at a specific site and for treatment of reproductive problems. Additionally, lipid-based NPs can be taken up by the tissues of patients with ovarian failure, deep endometriosis, testicul...
Source: Zygote - August 4, 2023 Category: Reproduction Medicine Authors: Pedro Alves Aguiar Barroso Danisv ânia Ripardo Nascimento Miguel F De Lima Neto Ernando Igo T De Assis Ciro Siqueira Figueira Jos é Roberto Viana Silva Source Type: research
Therapeutic potential of nanotechnology in reproduction disorders and possible limitations
Zygote. 2023 Aug 4:1-8. doi: 10.1017/S0967199423000424. Online ahead of print.ABSTRACTOne of the prominent peculiarities of nanoparticles (NPs) is their ability to cross biological barriers. Therefore, the development of NPs with different properties has great therapeutic potential in the area of reproduction because the association of drugs, hormones and other compounds with NPs represents an alternative for delivering substances directly at a specific site and for treatment of reproductive problems. Additionally, lipid-based NPs can be taken up by the tissues of patients with ovarian failure, deep endometriosis, testicul...
Source: Zygote - August 4, 2023 Category: Reproduction Medicine Authors: Pedro Alves Aguiar Barroso Danisv ânia Ripardo Nascimento Miguel F De Lima Neto Ernando Igo T De Assis Ciro Siqueira Figueira Jos é Roberto Viana Silva Source Type: research
Therapeutic potential of nanotechnology in reproduction disorders and possible limitations
Zygote. 2023 Aug 4:1-8. doi: 10.1017/S0967199423000424. Online ahead of print.ABSTRACTOne of the prominent peculiarities of nanoparticles (NPs) is their ability to cross biological barriers. Therefore, the development of NPs with different properties has great therapeutic potential in the area of reproduction because the association of drugs, hormones and other compounds with NPs represents an alternative for delivering substances directly at a specific site and for treatment of reproductive problems. Additionally, lipid-based NPs can be taken up by the tissues of patients with ovarian failure, deep endometriosis, testicul...
Source: Zygote - August 4, 2023 Category: Reproduction Medicine Authors: Pedro Alves Aguiar Barroso Danisv ânia Ripardo Nascimento Miguel F De Lima Neto Ernando Igo T De Assis Ciro Siqueira Figueira Jos é Roberto Viana Silva Source Type: research
Actin-mediated endocytosis in the E-YSL helps drive epiboly in zebrafish
Zygote. 2023 Aug 3:1-10. doi: 10.1017/S0967199423000357. Online ahead of print.ABSTRACTIn zebrafish, a punctate band of F-actin is reported to develop in the external yolk syncytial layer (E-YSL) during the latter part of epiboly in zebrafish embryos. Here, electron microscopy (EM) and fluorescence confocal microscopy were conducted to investigate dynamic changes in the E-YSL membrane during epiboly. Using scanning EM, we report that the surface of the E-YSL is highly convoluted, consisting of a complex interwoven network of branching membrane surface microvilli-like protrusions. The region of membrane surface protrusions ...
Source: Zygote - August 3, 2023 Category: Reproduction Medicine Authors: Jackie C Cheng Andrew L Miller Sarah E Webb Source Type: research
Actin-mediated endocytosis in the E-YSL helps drive epiboly in zebrafish
Zygote. 2023 Aug 3:1-10. doi: 10.1017/S0967199423000357. Online ahead of print.ABSTRACTIn zebrafish, a punctate band of F-actin is reported to develop in the external yolk syncytial layer (E-YSL) during the latter part of epiboly in zebrafish embryos. Here, electron microscopy (EM) and fluorescence confocal microscopy were conducted to investigate dynamic changes in the E-YSL membrane during epiboly. Using scanning EM, we report that the surface of the E-YSL is highly convoluted, consisting of a complex interwoven network of branching membrane surface microvilli-like protrusions. The region of membrane surface protrusions ...
Source: Zygote - August 3, 2023 Category: Reproduction Medicine Authors: Jackie C Cheng Andrew L Miller Sarah E Webb Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Evaluation of sperm and hormonal assessments in Wagyu, Nellore, and Angus bulls
This study aimed to evaluate whether there were differences in the hormone and sperm characteristics of Wagyu bulls compared with the same characteristics of subspecies Bos indicus and Bos taurus sires. Frozen-thawed semen from Wagyu, Nellore, and Angus sires were analyzed for sperm kinetics (computer-assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity, lipid peroxidation and hormone [luteinizing hormone (LH) and testosterone] serum concentration. The results showed that Wagyu had lower total motility and an increased number of sperm with no motility when compar...
Source: Zygote - July 26, 2023 Category: Reproduction Medicine Authors: A R Moura A R Santos J D A Losano A F P Siqueira T R S Hamilton R Zanella K C Caires R Sim ões Source Type: research
Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling
Zygote. 2023 Jul 24:1-9. doi: 10.1017/S0967199423000369. Online ahead of print.ABSTRACTPlacental trophoblastic cells play important roles in placental development and fetal health. However, the mechanism of trophoblastic cell fusion is still not entirely clear. The level of Tspan5 in the embryo culture medium was detected using enzyme-linked immunosorbent assay (ELISA). Fusion of BeWo cells was observed by immunofluorescence. Cell fusion-related factors and EMT-related factors were identified by qRT-PCR and western blotting. Notch protein repressor DAPT was used to verify the role of Tspan5 in BeWo cells. The expression of...
Source: Zygote - July 24, 2023 Category: Reproduction Medicine Authors: Hai-Yu Tang Mei Lin Yong-Qian Liang Jin-Hua Wang Hong-Gan Yi Man Yang Source Type: research
Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling
Zygote. 2023 Jul 24:1-9. doi: 10.1017/S0967199423000369. Online ahead of print.ABSTRACTPlacental trophoblastic cells play important roles in placental development and fetal health. However, the mechanism of trophoblastic cell fusion is still not entirely clear. The level of Tspan5 in the embryo culture medium was detected using enzyme-linked immunosorbent assay (ELISA). Fusion of BeWo cells was observed by immunofluorescence. Cell fusion-related factors and EMT-related factors were identified by qRT-PCR and western blotting. Notch protein repressor DAPT was used to verify the role of Tspan5 in BeWo cells. The expression of...
Source: Zygote - July 24, 2023 Category: Reproduction Medicine Authors: Hai-Yu Tang Mei Lin Yong-Qian Liang Jin-Hua Wang Hong-Gan Yi Man Yang Source Type: research
