Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling
Zygote. 2023 Jul 24:1-9. doi: 10.1017/S0967199423000369. Online ahead of print.ABSTRACTPlacental trophoblastic cells play important roles in placental development and fetal health. However, the mechanism of trophoblastic cell fusion is still not entirely clear. The level of Tspan5 in the embryo culture medium was detected using enzyme-linked immunosorbent assay (ELISA). Fusion of BeWo cells was observed by immunofluorescence. Cell fusion-related factors and EMT-related factors were identified by qRT-PCR and western blotting. Notch protein repressor DAPT was used to verify the role of Tspan5 in BeWo cells. The expression of...
Source: Zygote - July 24, 2023 Category: Reproduction Medicine Authors: Hai-Yu Tang Mei Lin Yong-Qian Liang Jin-Hua Wang Hong-Gan Yi Man Yang Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
Comparison of the duration of estradiol administration and the effect on pregnancy outcome of day 3 vitrified-warmed embryo transfer cycle: a randomized controlled trial
Zygote. 2023 Jul 14:1-7. doi: 10.1017/S096719942300031X. Online ahead of print.ABSTRACTBased on the fact that the follicular phase in the menstrual cycle has length variation, it has been assumed that the duration of oestrogen (E2) administration could also be variable; therefore, for the first time, this randomized clinical trial study was conducted to investigate and compare the duration of estradiol administration and the effect on pregnancy outcomes in the cleavage-stage frozen embryo transfer (FET) cycle. We included women aged 20-40 with a normal uterus on hysteroscopy between September and December 2022 and who were...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Afsoon Zarei Ameneh Keshavarzi Maryam Zare Source Type: research
Selection of < em > Rattus norvegicus < /em > cumulus-oocyte complex for vitrification by brilliant cresyl blue
The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(-), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control ...
Source: Zygote - July 14, 2023 Category: Reproduction Medicine Authors: Iaskara Oliveira Joana Fisch Juliana Gomes Rui Fernando Felix Lopes Alexandre Tavares Duarte de Oliveira Source Type: research
