Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration
Zygote. 2024 Jan 4:1-6. doi: 10.1017/S0967199423000618. Online ahead of print.ABSTRACTDespite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical-biological disciplines. In view of the above caveats, the aim of the study was to co...
Source: Zygote - January 4, 2024 Category: Reproduction Medicine Authors: Francisco Vergara Javier Fern ández Concepci ón Pedrosa Mar ía Muñoz Elvira Jerez Mireia Var ón Carmen Moyano Alberto Yoldi Jordi Ponce Source Type: research
Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration
Zygote. 2024 Jan 4:1-6. doi: 10.1017/S0967199423000618. Online ahead of print.ABSTRACTDespite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical-biological disciplines. In view of the above caveats, the aim of the study was to co...
Source: Zygote - January 4, 2024 Category: Reproduction Medicine Authors: Francisco Vergara Javier Fern ández Concepci ón Pedrosa Mar ía Muñoz Elvira Jerez Mireia Var ón Carmen Moyano Alberto Yoldi Jordi Ponce Source Type: research
Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration
Zygote. 2024 Jan 4:1-6. doi: 10.1017/S0967199423000618. Online ahead of print.ABSTRACTDespite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical-biological disciplines. In view of the above caveats, the aim of the study was to co...
Source: Zygote - January 4, 2024 Category: Reproduction Medicine Authors: Francisco Vergara Javier Fern ández Concepci ón Pedrosa Mar ía Muñoz Elvira Jerez Mireia Var ón Carmen Moyano Alberto Yoldi Jordi Ponce Source Type: research
Comparative study of intracytoplasmic sperm injection using the traditional holding and the oocyte-holding pipette without aspiration
Zygote. 2024 Jan 4:1-6. doi: 10.1017/S0967199423000618. Online ahead of print.ABSTRACTDespite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical-biological disciplines. In view of the above caveats, the aim of the study was to co...
Source: Zygote - January 4, 2024 Category: Reproduction Medicine Authors: Francisco Vergara Javier Fern ández Concepci ón Pedrosa Mar ía Muñoz Elvira Jerez Mireia Var ón Carmen Moyano Alberto Yoldi Jordi Ponce Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Ultrastructural study: < em > in vitro < /em > and < em > in vivo < /em > differentiation of mice spermatogonial stem cells
In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitr...
Source: Zygote - December 27, 2023 Category: Reproduction Medicine Authors: Zahra Bashiri Mansoureh Movahedin Vahid Pirhajati Hamidreza Asgari Morteza Koruji Source Type: research
Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women
In conclusion, malnutrition and other conditions in underweight women may adversely affect ovulation, and the development, and fertilization of oocytes resulting from changes to the intracellular structure of cumulus cells and metabolic processes. These changes may lead to reduced fertility or unsatisfactory reproduction outcomes in women.PMID:38130161 | DOI:10.1017/S0967199423000588 (Source: Zygote)
Source: Zygote - December 22, 2023 Category: Reproduction Medicine Authors: Hong Ji Qing Zhang Lu Ding Rongjuan Chen Fu Liu Ping Li Source Type: research
Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women
In conclusion, malnutrition and other conditions in underweight women may adversely affect ovulation, and the development, and fertilization of oocytes resulting from changes to the intracellular structure of cumulus cells and metabolic processes. These changes may lead to reduced fertility or unsatisfactory reproduction outcomes in women.PMID:38130161 | DOI:10.1017/S0967199423000588 (Source: Zygote)
Source: Zygote - December 22, 2023 Category: Reproduction Medicine Authors: Hong Ji Qing Zhang Lu Ding Rongjuan Chen Fu Liu Ping Li Source Type: research
Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women
In conclusion, malnutrition and other conditions in underweight women may adversely affect ovulation, and the development, and fertilization of oocytes resulting from changes to the intracellular structure of cumulus cells and metabolic processes. These changes may lead to reduced fertility or unsatisfactory reproduction outcomes in women.PMID:38130161 | DOI:10.1017/S0967199423000588 (Source: Zygote)
Source: Zygote - December 22, 2023 Category: Reproduction Medicine Authors: Hong Ji Qing Zhang Lu Ding Rongjuan Chen Fu Liu Ping Li Source Type: research
Structural and metabolic cumulus cell alteration affects oocyte quality in underweight women
In conclusion, malnutrition and other conditions in underweight women may adversely affect ovulation, and the development, and fertilization of oocytes resulting from changes to the intracellular structure of cumulus cells and metabolic processes. These changes may lead to reduced fertility or unsatisfactory reproduction outcomes in women.PMID:38130161 | DOI:10.1017/S0967199423000588 (Source: Zygote)
Source: Zygote - December 22, 2023 Category: Reproduction Medicine Authors: Hong Ji Qing Zhang Lu Ding Rongjuan Chen Fu Liu Ping Li Source Type: research
