The first evidence of intersexuality in Atka mackerel Pleurogrammus monopterygius (Hexagrammidae).
Abstract Histological examination of Atka mackerel ovotestes reveals the possibility of intersexuality. Individuals with bisexual gonads have been caught in the North Pacific near the south-east coast of the Kamchatka peninsula. While intersex appeared to be normal females with developing ovaries, histological analysis showed the presence of both female and male tissue in the same gonad. Specifically, primary growth, cortical alveolar and primary vitellogenic oocytes were located among spermatogonia cysts. The prevalence of intersexuality in the population was less than 0.1%. PMID: 33446286 [PubMed - as suppl...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Zhukova K, Golovatyuk GY Tags: Zygote Source Type: research
Induced reproduction and early development in dourado, Salminus franciscanus Lima & Britski, 2007 (Pisces: Characiformes).
Induced reproduction and early development in dourado, Salminus franciscanus Lima & Britski, 2007 (Pisces: Characiformes). Zygote. 2021 Jan 15;:1-6 Authors: Paschoalini AL, Eloi MR, Dos Santos JE, Dos Santos JCE, Rizzo E, Bazzoli N Abstract Our study aimed to establish the response of Salminus franciscanus to hypophysation and describe the main morphological events of its embryonic process. Wild fish were captured in São Francisco River and selected broodstock (females: 66.4 ± 11.1 cm and 4.04 ± 2.32 kg; males: 58.3 ± 10.2 cm and 3.62 ± 1.12 kg) were kept at 26.1...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Paschoalini AL, Eloi MR, Dos Santos JE, Dos Santos JCE, Rizzo E, Bazzoli N Tags: Zygote Source Type: research
Short-term storage of Chinese sturgeon (Acipenser sinensis) ova in vitro.
In this study, we tried to maintain the vitality of Chinese sturgeon (Acipenser sinensis) ova before fertilization with several treatments in vitro. The ovulated eggs were allocated to groups with different incubation medium (coelomic fluid and artificial media), temperature (4°C and 16°C) and storage duration (2 h and 6 h). The maximum fertilization and hatching rate were observed for the control group in which the ova were fertilized immediately after spawning, with the values of 82.45% and 84.73%, respectively. Compared with the control group, the fertilization and hatching rate of all the treatment groups store...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Tan C, Liu X, Yang J, Liu J, Du H Tags: Zygote Source Type: research
Impact of captive conditions on female germinal epithelium of the butterflyfish Chaetodon striatus (Perciformes: Chaetodontidae).
Abstract Chaetodon striatus is a cosmopolitan seawater species present in aquaria all over the world and its extractivism is quite high. The lack of studies on the reproductive biology of C. striatus contributes to the difficulty in managing the species outside its natural habitat. Without knowledge of the mechanisms that control or affect gonadal changes, reproduction of C. striatus in captivity has become almost impossible, considering that the species is quite sensitive and the effect of captive conditions on its reproductive biology is unknown. Therefore, this study aimed to evaluate the effect on its reproduc...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Mazzoni TS, da Silva GC, Bayona Perez IL, Quagio-Grassiotto I Tags: Zygote Source Type: research
Optimization of ultraviolet irradiation of Clarias gariepinus (Burchell, 1822) eggs for androgen production.
Abstract The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C. gariepinus and cold shocked at 5°C for 5 min just moments before cell cleavage. Ploidy analysis was performed using karyotype chromosome counting. The results obtained suggested that the further the distance, the better ...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Okomoda VT, Nursyaza HJ, Samuel IO, Hassan A, Oladimeji AS, Abol-Munafi AB, Alabi KI, Ikhwanuddin M Tags: Zygote Source Type: research
Inseminating dose for the artificial fertilization of Brycon amazonicus (Teleostei: Characidae).
Abstract Knowledge of the sperm-oocyte ratio in fish fertilization serves as the basis for studies on artificial reproduction and gamete manipulation. The aim of this study was to determine the minimum insemination dose for Brycon amazonicus oocyte fertilization. Female and male gametes were used and tested with the following doses of spermatozoa oocyte-1 ml-1: 10,000, 20,000, 40,000, 60,000 and 80,000 (in triplicate). Fertilization rates were calculated and estimated from the regression equation by applying the segmented regression model 'Linear Response Plateau' to determine the appropriate proportion of gametes...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Oliveira RGS, Villacorta-Correa MA Tags: Zygote Source Type: research
Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation.
Abstract In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controll...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Rola LD, Zanetti EDS, Del Collado M, Peroni EFC, Duarte JMB Tags: Zygote Source Type: research
Sperm chromatin protamination influences embryo development in unsexed and sexed bull semen.
hi OM Abstract Sex selection through sperm sorting offers advantages in regards selection pressure in high-producing livestock. However, the sex-sorting process results in sperm membrane and DNA damage that ultimately decrease fertility. We hypothesized that given the role of protamines in DNA packaging, protamine deficiency could account, at least partially, for the DNA damage observed following sperm sex sorting. To test this, we compared protamine status between unsexed and sexed spermatozoa from two bulls using the fluorochrome chromomycin A3 (CMA3) and flow cytometry. Then, we assessed embryo development foll...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Silva TVG, Santana PDPB, Souza EB, Lima AJM, Santos CA, Almeida NNDC, Brito VC, Gonçalves AA, Rolim Filho ST, Cordeiro MDS, Santos SDSD, Miranda MDS, Ohashi OM Tags: Zygote Source Type: research
C57BL/6J mouse superovulation: schedule and age optimization to increase oocyte yield and reduce animal use.
In this study, we aimed to optimize a superovulation protocol by injecting 5 IU of pregnant mare serum gonadotropin followed by 5 IU of hCG 48 h later, using three different schedules related to the beginning of the dark cycle (3, 5 and 7 pm) in a light cycle of 7 am to 7 pm, with light on at 7 am. C57BL/6J mice at 3, 4 and 5 weeks of age were used and the estrous cycle phase for times of PMSG and hCG injections was also analyzed. Total oocyte number was counted in the morning after hCG injection. Hormones given at 3 weeks of age at 3 pm (59 ± 15 oocytes) and 7 pm (61 ± 10 oocytes) produced a significantly hi...
Source: Zygote - January 15, 2021 Category: Reproduction Medicine Authors: Lamas S, Carvalheira J, Gartner F, Amorim I Tags: Zygote Source Type: research
Effect of antioxidants on preimplantation embryo development in vitro: a review.
Abstract In vitro culture of the embryo is a useful method to treat infertility that shows embryo potential for selecting the best one to transfer and successfully implantation. However, embryo development in vitro is affected by oxidative stresses such as reactive oxygen species that may damage embryo development. Antioxidants are molecules found in fruits, vegetables, and fish that play an important role in reducing oxidative processes. In the natural environment, there is a physiological antioxidant system that protects embryos against oxidative damage. This antioxidant system does not exist in vitro. Antioxida...
Source: Zygote - January 14, 2021 Category: Reproduction Medicine Authors: Zarbakhsh S Tags: Zygote Source Type: research
Embryonic development of the fire-eye-tetra Moenkhausia oligolepis (Characiformes: Characidae).
This study describes the embryonic development of Moenkhausia oligolepis in laboratory conditions. After fertilization, the embryos were collected every 10 min up to 2 h, then every 20 min up to 4 h, and afterwards every 30 min until hatching. The fertilized eggs of M. oligolepis measured approximately 0.85 ± 0.5 mm and had an adhesive surface. Embryonic development lasted 14 h at 25ºC through the zygote, cleavage, blastula, gastrula, neurula, and segmentation phases. Hatching occurred in embryos around the 30-somites stage. The present results contribute only the second description of embryonic development to ...
Source: Zygote - January 12, 2021 Category: Reproduction Medicine Authors: Dos Santos RS, Rodrigues JR, Cordeiro JG, Tercya H, Leite M, Costa BPD, da Silva Costa R, Maximino C, Siqueira-Silva DH Tags: Zygote Source Type: research
DNA methyltransferase inhibitors modulate histone methylation: epigenetic crosstalk between H3K4me3 and DNA methylation during sperm differentiation.
Abstract The process of cytodifferentiation in spermatogenesis is governed by a unique genetic and molecular programme. In this context, accurate 'tuning' of the regulatory mechanisms involved in germ cells differentiation is required, as any error could have dramatic consequences on species survival and maintenance. To study the processes that govern the spatial-temporal expression of genes, as well as analyse transmission of epigenetic information to descendants, an integrated approach of genetics, biochemistry and cytology data is necessary. As information in the literature on interplay between DNA methylation ...
Source: Zygote - January 8, 2021 Category: Reproduction Medicine Authors: Burlibaşa L, Nicu AT, Domnariu C Tags: Zygote Source Type: research
Should fertile women quit drinking alcohol to produce better quality oocytes?
In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age
Source: Zygote - December 18, 2020 Category: Reproduction Medicine Authors: Ozbakir B, Tulay P Tags: Zygote Source Type: research
Effect of cyanocobalamin on oocyte maturation, in vitro fertilization, and embryo development in mice.
Abstract The aim of this study was to investigate the effect of cyanocobalamin supplementation on in vitro maturation (IVM), in vitro fertilization (IVF), and subsequent embryonic development competence to the blastocyst stage, and in vitro development of mouse 2-cell embryos. Cumulus cells were prepared from mouse cumulus-oocyte complexes (COCs) and incubated for 24 h in an in vitro culture (IVC) medium that contained different concentrations of cyanocobalamin (100, 200, 300 or 500 pM). We collected 2-cell embryos from superovulated NMRI mice and cultured them in the same concentrations of cyanocobalamin (100, 20...
Source: Zygote - December 17, 2020 Category: Reproduction Medicine Authors: Rostami T, Fathi F, Assadollahi V, Hosseini J, Khadem Erfan MB, Rashidi A, Amiri G, Banafshi O, Alasvand M Tags: Zygote Source Type: research
Transferrin receptor (TFRC) is essential for meiotic progression during mouse spermatogenesis.
In this study, we aimed to determine the function of the TFRC in neonatal testicular development by TFRC knockdown using the testis culture platform. Our results showed high TFRC expression in 2-week testes, corresponding to the first meiotic division. Targeting TFRC using morpholino oligonucleotides resulted in clear spermatocyte apoptosis. In addition, we used the chromosomal spread technique to show that a deficiency of TFRC caused the accumulation of leptotene and zygotene spermatocytes, and a decrease of pachytene spermatocytes, indicating early meiotic arrest. Moreover, the chromosomes of TFRC-deficient pachytene spe...
Source: Zygote - December 16, 2020 Category: Reproduction Medicine Authors: Gao T, Lin M, Wu Y, Li K, Liu C, Zhou Q, Shen C, Zheng B, Huang X Tags: Zygote Source Type: research
Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles.
Abstract The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. ...
Source: Zygote - November 25, 2020 Category: Reproduction Medicine Authors: Ang L, Haixia C, Hongxia L, Ruijiao L, Xingping G, Huaixiu W Tags: Zygote Source Type: research
Use of time-lapse monitoring in medically assisted reproduction treatments: a mini-review.
Abstract During human in vitro culture, a morphological microscope analysis is normally performed to select the best embryo to transfer, with the hope of obtaining a successful pregnancy. The morphological evaluation may combine number and size of blastomeres, fragmentation, multinucleation, blastocyst expansion, inner-cell mass and trophectoderm appearance. However, standard microscopy evaluation involves the removal of the embryos from the incubator, exposing them to changes in pH, temperature, and oxygen level. Additionally, morphological assessments might include high inter-observer variability. Recently, cont...
Source: Zygote - November 24, 2020 Category: Reproduction Medicine Authors: Sciorio R Tags: Zygote Source Type: research
Ovarian activity and antioxidant indices during estrous cycle of Barki ewes under effect of thyme, celery and salinomycin as feed additives.
Abstract This research aimed to examine the effects of thyme, celery and salinomycin on ovarian sex hormones, reproductive traits and antioxidant status during the estrous cycle. Seventy-five mature Barki ewes aged 2-3 years with an average weight of 40 ± 1.5 kg were assigned randomly into five groups (15 head/group). Group 1 was kept as the control; groups 2 and 3 received 20 g/head/day thyme (T) and celery (C) as dried herbs, respectively. Group 4 (T×C) received 10 g thyme + 10 g celery/head/day, and group 5 was treated with salinomycin 1 g/head/day. Blood samples were collected during follicular an...
Source: Zygote - November 24, 2020 Category: Reproduction Medicine Authors: El-Zaher HM, Eid SY, Shaaban MM, Ahmed-Farid OA, Abd El Tawab AM, Khattab MSA Tags: Zygote Source Type: research
lncRNA SNHG14 involved in trophoblast cell proliferation, migration, invasion and epithelial-mesenchymal transition by targeting miR-330-5p in preeclampsia.
This study aimed to determine if lncRNA SNHG14 has a protective effect on placental trophoblast and prevents PE. SNHG14 levels in the peripheral blood from patients with PE or from women with healthy pregnancies were detected using RT-qPCR. The relationship between SNHG14 and miR-330-5p was determined using a dual-luciferase reporter assay. In addition, cell proliferation and cell cycle were evaluated by performing CCK8 assays and flow-cytometric analysis, respectively. Wound-healing and transwell assays were performed to assess cell migration and invasion ability. lncRNA SNHG14 was downregulated in PE patients; it was inv...
Source: Zygote - November 9, 2020 Category: Reproduction Medicine Authors: Zhang Y, Zhang M Tags: Zygote Source Type: research
Evaluation of extracellular vesicles and gDNA from culture medium as a possible indicator of developmental competence in human embryos.
rez L Abstract Human embryos generated in vitro have a high incidence of chromosomal abnormalities that negatively affect pregnancy rate. Embryos generated in vitro secrete extracellular vesicles (EVs) into the culture medium that could be used potentially as indicators of embryo competence. This research aimed to evaluate the concentration and size of EVs and their gDNA content as an indicator of developmental competence in human embryos. Human embryos generated by intracytoplasmic sperm injection (ICSI) were classified morphologically as of either TOP, FAIR or POOR quality. Culture medium and developmentally arr...
Source: Zygote - October 29, 2020 Category: Reproduction Medicine Authors: Veraguas D, Aguilera C, Henriquez C, Velasquez AE, Melo-Baez B, Silva-Ibañez P, Castro FO, Rodriguez-Alvarez L Tags: Zygote Source Type: research
Embryo co-culture with bovine amniotic membrane stem cells can enhance the cryo-survival of IVF-derived bovine blastocysts comparable with co-culture with bovine oviduct epithelial cells.
Abstract Culture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then ...
Source: Zygote - October 20, 2020 Category: Reproduction Medicine Authors: Nejat-Dehkordi S, Ahmadi E, Shirazi A, Nazari H, Shams-Esfandabadi N Tags: Zygote Source Type: research
Advances in the study of zygote activation in higher plants.
Abstract In higher plants, fertilization induces many structural and physiological changes in the fertilized egg that reflect the transition from the haploid female gamete to the diploid zygote - the first cell of the sporophyte. After fusion of the egg nucleus with the sperm nucleus, many molecular changes occur in the zygote during the process of zygote activation during embryogenesis. The zygote originates from the egg, from which some pre-stored translation initiation factors transfer into the zygote and function during zygote activation. This indicates that the control of zygote activation is pre-set in the e...
Source: Zygote - October 15, 2020 Category: Reproduction Medicine Authors: Li DX, Chen SJ, Tian HQ Tags: Zygote Source Type: research
Oxidative stress caused by a dysregulated Wnt/ β-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome.
In this study, we found that CFS decreased the number of implantation sites for blastocysts, and increased the number of absorbed, stillborn and malformed fetuses. The morphology and structure of the placenta were abnormal in pregnant mice with CFS. Further study found that the oxidative stress in serum, uterus and placenta was increased in pregnant mice with CFS, while the levels of antioxidase were decreased. CFS also inhibited the Wnt/β-catenin signalling pathway in the placenta. These results suggested that inhibition of the Wnt/β-catenin signalling pathway and enhanced oxidative stress play an important role...
Source: Zygote - October 15, 2020 Category: Reproduction Medicine Authors: Zhao H, Zhang J, Qian N, Wu S, Wu Y, Yao G Tags: Zygote Source Type: research
5-Alfa reductase type 2 (SRD5A2) gene rs523349 polymorphism is not associated with non-obstructive azoospermia in Turkish patients.
Abstract Spermatogenesis is an androgen-dependent event, and testosterone is the major androgen source. The enzyme 5-alpha reductase converts testosterone to dihydrotestosterone (DHT) in testicular and peripheral tissues. Polymorphisms in genes encoding 5-alpha reductase may be associated with impaired male fertility. The present study aimed to investigate the relationship between 5-alpha reductase type 2 (SRD5A2) gene rs523349 polymorphism and non-obstructive azoospermia (NOA) in Turkish patients. The study included 75 NOA patients and 43 fertile men from Turkey. No significant relationship was found between SRD5...
Source: Zygote - October 14, 2020 Category: Reproduction Medicine Authors: Serdarogullari M, Coban O, Yarkiner Z, Gulec Yilmaz S, Akdeniz T, Isbir T Tags: Zygote Source Type: research
Does the number of embryos loaded on a single cryo-carrier affect post-vitrification survival rate?
Abstract BACKGROUND: We aimed to assess whether the survival rate of embryos is influenced by the number of embryos/oocytes loaded on a single cryo-carrier during vitrification. METHODS: This was a retrospective study that included 974 patients who underwent thawing of 1896 embryo-warming cycles between September 2016 and January 2020. A distinct analysis was made for cleavage stage embryos (2-10-cell stage) and blastocysts. For vitrification, embryos were placed in a Cryotop™ open device using a SAGE vitrification kit following the manufacturer's instructions. Warming was carried using a SAGE warming v...
Source: Zygote - October 13, 2020 Category: Reproduction Medicine Authors: Aizer A, Noach-Hirsh M, Dratviman-Storobinsky O, Haas J, Orvieto R Tags: Zygote Source Type: research
CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation.
Abstract Mutation in CFAP43 leads to severe asthenozoospermia and multiple morphological abnormalities of the sperm flagellum (MMAF) in both human and mouse. Previous studies have shown that disruption of intra-manchette transport (IMT) caused failure of flagellum assembly and sperm head shaping. In a previous study, therefore, we postulated that disruption of IMT may contribute to the failure of sperm flagellum formation and result in MMAF, however the mechanisms underlying these defects are still poorly understood. Cfap43-deficient mice were studied here to reveal the cellular mechanisms of abnormal sperm head m...
Source: Zygote - October 13, 2020 Category: Reproduction Medicine Authors: Yu Y, Wang J, Zhou L, Li H, Zheng B, Yang S Tags: Zygote Source Type: research
Ram semen quality can be assessed by flow cytometry several hours after post-fixation.
te;k R, Macháč M, Chrenek P Abstract Ram spermatozoa are very sensitive to any cold shock or oxidative damage, therefore making them unsuitable for prolonged storage or distant transport to specialized laboratories for flow-cytometric analysis. The aim of this study was to stain ram semen samples with several fluorescent markers and analyse their stability during formaldehyde fixation. Briefly, freshly collected semen samples were stained for apoptosis (annexin V-FITC, YO-PRO™-1 and FLICA), acrosomal damage (PNA-AF488 and FITC-conjugated antibody against GAPDHS), mitochondrial activity (Mitotracker p...
Source: Zygote - October 13, 2020 Category: Reproduction Medicine Authors: Vašíček J, Svoradová A, Baláži A, Jurčík R, Macháč M, Chrenek P Tags: Zygote Source Type: research
Oviductal high concentration of K+ suppresses hyperpolarization but does not prevent hyperactivation, acrosome reaction and in vitro fertilization in hamsters.
Abstract Mammalian sperm have to undergo capacitation to be fertilization competent. Capacitated sperm in vitro show hyperpolarization of the membrane potential. It has been reported that in mouse membrane hyperpolarization is necessary for the acrosome reaction. We recently found that the fluid of the hamster oviduct, where fertilization occurs, contained a high potassium (K+) concentration (~20 mEq/l). This high K+ concentration could depolarize the membrane potential and prevent the acrosome reaction/fertilization. Conversely, some beneficial effects on capacitation of high K+ concentration or a high K/Na ratio...
Source: Zygote - October 4, 2020 Category: Reproduction Medicine Authors: Takei GL, Kon H Tags: Zygote Source Type: research
Reproductive strategies and chromosomal aberrations affect survival in the Rivuliid fish Hypsolebias sertanejo.
Abstract Rivulidae comprises a family of fish largely distributed in Brazil that includes 201 species, of which 125 are considered endangered. This fact emphasizes the need for development of conservation strategies including studies on genetics and reproduction. In this paper, we describe aspects of biology and reproduction of the rivuliid species Hypsolebias sertanejo. We outline the reproductive behaviour of this species under laboratory conditions, analyze ploidy status by flow cytometry, describe reproductive behaviour and performance and test dry and wet incubation of eggs. Although H. sertanejo showed well ...
Source: Zygote - October 4, 2020 Category: Reproduction Medicine Authors: Evangelista MM, Romagosa E, Siqueira-Silva DH, Yasui GS, Fujimoto T, Senhorini JA Tags: Zygote Source Type: research
Parthenogenetic activation of buffalo (Bubalus bubalis) oocytes: comparison of different activation reagents and different media on their developmental competence and quantitative expression of developmentally regulated genes.
This study was carried out to compare the efficacy of different methods to activate buffalo A + B and C + D quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. The percentage of A + B oocytes (62.16 ± 5.06%, range 53.8-71.3%) was significantly higher (P
Source: Zygote - October 1, 2020 Category: Reproduction Medicine Authors: Singh KP, Mohapatra SK, Kaushik R, Singh MK, Palta P, Singla SK, Manik RS, Chauhan MS Tags: Zygote Source Type: research
Factors related to follicular oxidative stress in intracytoplasmic sperm injection cycles and its effects on granulosa cells.
Abstract The aim of the present study was to investigate several common conditions that may potentially be correlated with follicular oxidative status during an intracytoplasmic sperm injection (ICSI) cycle and that include the serum oestrogen level on the day of oocyte pick-up, maternal age and pregnancy outcome. Patients that were enrolled in the study were classified randomly into three groups using their numerical order. The first group were classified based on maternal age (90th percentile and ≤ 90th percentile) (n = 491) and the third group on pregnancy outcome (positive/negative) (n = 376). The groups we...
Source: Zygote - September 27, 2020 Category: Reproduction Medicine Authors: Karabulut S, Korkmaz O, Kutlu P, Keskin I Tags: Zygote Source Type: research
Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae.
Abstract The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock t...
Source: Zygote - September 23, 2020 Category: Reproduction Medicine Authors: Martins LF, Hilbig CC, Yasui GS, Monzani PS, Senhorini JA, Nakaghi LSO, do Nascimento NF Tags: Zygote Source Type: research
Porcine follicular fluid derived from > 8 mm sized follicles improves oocyte maturation and embryo development during in vitro maturation of pigs.
Porcine follicular fluid derived from> 8 mm sized follicles improves oocyte maturation and embryo development during in vitro maturation of pigs. Zygote. 2020 Sep 22;:1-6 Authors: Park JE, Lee SH, Hwangbo Y, Park CK Abstract The aim of the present study was to investigate the effects of porcine follicular fluid (pFF) from large-sized (LFF;>8 mm in diameter) and medium-sized (MFF; 3-6 mm in diameter) follicles on the maturation and developmental competence of porcine oocytes. Cumulus-oocyte complexes (COCs) were collected from follicles 3-6 mm in diameter. The collected COCs were incubated for 22...
Source: Zygote - September 21, 2020 Category: Reproduction Medicine Authors: Park JE, Lee SH, Hwangbo Y, Park CK Tags: Zygote Source Type: research
Spermatozoa morphology changes during reproduction and first observation of acrosomal contact in two dioecious species of Macrobiotidae (Tardigrada: Eutardigrada).
Abstract Mating behaviours for two species of dioecious eutardigrades: a strain of Paramacrobiotus sp. and Macrobiotus shonaicus (Stec et al., 2018) have been recorded previously, and observations have indicated that spermatozoa of both species are first released into the environment, then swim through the cloaca of the females and into the spermatheca. The fusion of gamete nuclei has not yet occurred in a laid egg. Therefore, it has been suggested that fertilization is completed externally as the egg is released into the environment before the nuclei of the gametes fuse. In the present study, the spermatozoa of b...
Source: Zygote - September 10, 2020 Category: Reproduction Medicine Authors: Sugiura K, Matsumoto M Tags: Zygote Source Type: research
Methodological approaches for vitrification of bovine oocytes.
á E, Strejček F Abstract Numerous factors affect vitrification success and post-thaw development of oocytes after in vitro fertilization. Therefore, elaboration of an optimal methodology ensuring higher cryotolerance of oocytes and subsequent blastocyst yield is still of great interest. This paper describes and evaluates critical factors affecting the success of oocyte vitrification. In particular, an appropriate oocyte stage such as maturation status (germinal vesicle stage, metaphase II stage), presence/absence of cumulus cells before vitrification, and the effect of follicle size, as well as different c...
Source: Zygote - September 3, 2020 Category: Reproduction Medicine Authors: Dujíčková L, Makarevich AV, Olexiková L, Kubovičová E, Strejček F Tags: Zygote Source Type: research
Frozen-thawed cleavage stage versus blastocyst stage embryo transfer in high responder patients.
Abstract Clinical outcomes following frozen-thawed cleavage embryo transfer versus frozen-thawed blastocyst transfer in high responder patients undergoing in vitro fertilisation/intracytoplasmic sperm injection cycles are still debated. In a retrospective study, 106 high responder patients who were candidate for 'freeze-all embryos' were recruited. Frozen-thawed embryos were transferred at the cleavage stage (n = 53) or the blastocyst stage (n = 53). Clinical pregnancy was considered as the primary outcome and chemical pregnancy, ongoing pregnancy, implantation rate, and fertilization rate, as well as miscarriage ...
Source: Zygote - August 26, 2020 Category: Reproduction Medicine Authors: Eftekhar M, Mohammadi B, Tabibnejad N, Mortazavi Lahijani M Tags: Zygote Source Type: research
Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report.
We report the case of a couple with primary unexplained infertility with a history of previous failed, in vitro fertilization intracytoplasmic sperm injection (IVF-ICSI) cycles characterized by the presence of ZP-free oocytes. Whole exome sequencing (WES) was carried out to analyse the possible genetic basis of oocyte abnormality. RESULTS: Diagnostic ICSI provided information about the embryo development potential from ZP-free oocytes and allowed better planning of the subsequent ICSI cycle. WES revealed that the absence of ZP was likely to be due to a new (ZP1) mutation. The subsequent ICSI cycle resulted in the deli...
Source: Zygote - August 26, 2020 Category: Reproduction Medicine Authors: Metwalley A, Brasha N, Esteves SC, Fawzy M, Brasha H, Hellani A, El Hamshary M, Khamiss O Tags: Zygote Source Type: research
Effects of dexamethasone on growth, viability and ultrastructure of bovine secondary follicles cultured in vitro.
This study aimed to evaluate the effects of dexamethasone on development, viability, antrum formation and ultrastructural integrity of bovine secondary follicles cultured in vitro for 18 days. Bovine ovaries were obtained from slaughterhouses and secondary follicles of ~150-200 µm diameter were isolated and cultured in the laboratory in TCM-199+ alone or supplemented with different concentrations of dexamethasone (1, 10, 100 and 1000 ng/ml). Follicle viability was evaluated after the culture period, using calcein-AM (viable) and ethidium homodimer (nonviable). Follicle diameters and antrum formation were evaluated at...
Source: Zygote - August 26, 2020 Category: Reproduction Medicine Authors: Barroso PAA, Paulino LRFM, Silva BR, Vasconcelos GL, Gomes DS, Lima Neto MF, Silva AWB, Souza ALP, Donato MAM, Peixoto CA, Silva JRV Tags: Zygote Source Type: research
Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (G ünther, 1864).
Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864). Zygote. 2020 Aug 19;:1-6 Authors: Rodrigues J, Santos RSD, Cordeiro JG, Leite M, Oliveira HSTOE, Tercya H, Costa BPD, Nascimento NFD, Maximino C, de Siqueira-Silva DH Abstract The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to ...
Source: Zygote - August 18, 2020 Category: Reproduction Medicine Authors: Rodrigues J, Santos RSD, Cordeiro JG, Leite M, Oliveira HSTOE, Tercya H, Costa BPD, Nascimento NFD, Maximino C, de Siqueira-Silva DH Tags: Zygote Source Type: research
Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus-oocyte complexes.
This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus-oocyte complexes (COCs) after in vitro maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of in vitro matured bovine COCs was studied. A positive correlation (P
Source: Zygote - August 18, 2020 Category: Reproduction Medicine Authors: de Vasconcelos GL, Maculan R, da Cunha EV, Silva AWB, Batista ALS, Donato MAM, Peixoto CA, Silva JRV, de Souza JC Tags: Zygote Source Type: research
Eremomastax speciosa potentializes the PMSG-inducing effect on some physiological and biochemical parameters in PMSG-primed immature rats.
Abstract The present study evaluated the effect of the aqueous extract from leaves of E. speciosa on some physiological and biochemical parameters of reproduction and the onset of puberty in pregnant mare serum gonadotropin (PMSG)-primed immature female rats. High pressure liquid chromatography (HPLC) was used to quantify the phenolic compounds in the methanol/methylene chloride (1:1) extract, the ethanolic and ethyl acetate fractions and the aqueous residue of E. speciosa. E. speciosa (0, 8, 32 or 64 mg/kg) were administered for 15 days to 24 non-PMSG-primed and 24 primed rats with 0.01 IU of PMSG. At the end of ...
Source: Zygote - August 11, 2020 Category: Reproduction Medicine Authors: Mbemya GT, Goka MSC, Lienou LL, Sylvain NN, Donfack JN, Guerreiro DD, Njimou JR, Rodrigues APR, Telefo PB Tags: Zygote Source Type: research
Assessment of binder of sperm protein 1 (BSP1) and heparin effects on in vitro capacitation and fertilization of bovine ejaculated and epididymal sperm.
ra AA Abstract The present study evaluated the effect of binder of sperm protein 1 (BSP1) and/or heparin on in vitro bovine capacitation and fertilization rates using epididymal and ejaculated bovine sperm. Frozen-thawed sperm were selected and used in the following treatments. Control group: Fert-TALP medium without heparin; heparin (HEP) group: Fert-TALP with heparin (10 UI/ml); BSP1 group: Fert-TALP medium with BSP1 (10 µg/ml for ejaculated sperm; 40 µg/ml for epididymal sperm); HEP + BSP1 group: Fert-TALP medium with heparin (5 UI/ml) and BSP1 (5 µg/ml for ejaculated sperm; 20 µg/ml for...
Source: Zygote - August 9, 2020 Category: Reproduction Medicine Authors: Rodríguez-Villamil P, Mentz D, Ongaratto FL, Aguiar LH, Rodrigues JL, Bertolini M, Moura AA Tags: Zygote Source Type: research
Development of prepubertal goat oocytes after their in vitro maturation and chemical activation.
Abstract Experiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus-oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their ...
Source: Zygote - August 9, 2020 Category: Reproduction Medicine Authors: Hong S, Vettical BS, Wani NA Tags: Zygote Source Type: research
Study on embryonic and larval developmental stages of Sucker head Garra gotyla (Gray 1830; Teleostei; Cyprinidae).
Abstract Garra gotyla is an indigenous coldwater fish of the cyprinid family and has wide geographical distribution in India as well as in other countries of Asia and Africa. Induced breeding in G. gotyla was carried out successfully for the first time and an attempt has been made to document developmental stages chronologically from the first minute of fertilization, through all stages of embryonic development until the fifth day post hatching. This experiment was carried out at 22-24°C water temperature at the Directorate of Coldwater Fisheries Research, Bhimtal, India. During the breeding trial, the fertili...
Source: Zygote - August 9, 2020 Category: Reproduction Medicine Authors: Patiyal RS, Mir MI, Pandey N, Rajesh M, Sarma D, Chandra S Tags: Zygote Source Type: research
Effects of oxygen concentrations on developmental competence and transcriptomic profile of yak oocytes.
In this study, we investigated the effects of different oxygen concentrations (5% versus 20%) on the developmental competence, reactive oxygen species (ROS) levels, glutathione (GSH) content, and transcriptomic profile of yak oocytes. The results showed that a low oxygen concentration significantly increased the maturation rate of yak oocytes (81.2 ± 2.2% vs 75.9 ± 1.3%) and the blastocyst quality of yak in vitro fertilized embryos. Analysis of ROS and GSH showed that a low oxygen concentration reduced ROS levels and increased the content of GSH (75.05 ± 7.1 ng/oocyte vs 50.63 ± 5.6 ng/oocyte). ...
Source: Zygote - August 9, 2020 Category: Reproduction Medicine Authors: Li R, Luo Y, Xu J, Sun Y, Ma Z, Chen S Tags: Zygote Source Type: research
Developmental potential of somatic and germ cells of hybrids between Carassius auratus females and Hemigrammocypris rasborella males.
In this study, we produced allo-diploid hybrids by cross-fertilization between female goldfish (Carassius auratus) and male golden venus chub (Hemigrammocypris rasborella). Inviability of these hybrids was due to breakage of the enveloping layer during epiboly or due to malformation with serious cardiac oedema around the hatching stage. Spontaneous allo-triploid hybrids with two sets of the goldfish genome and one set of the golden venus chub genome developed normally and survived beyond the feeding stage. This improved survival was confirmed by generating heat-shock-induced allo-triploid hybrids that possessed an extra go...
Source: Zygote - August 9, 2020 Category: Reproduction Medicine Authors: Naya Y, Matsunaga T, Shimizu Y, Takahashi E, Shima F, Endoh M, Fujimoto T, Arai K, Yamaha E Tags: Zygote Source Type: research
Modulation of phosphatidylinositol 3-kinase activity during in vitro oocyte maturation increases the production of bovine blastocysts.
This study aimed to evaluate the effect of regulating phosphatidylinositol 3-kinase (PI3K) activity on the kinetics of oocyte nuclear maturation and the blastocyst rate. To evaluate oocyte viability, nuclear maturation rate and in vitro embryo production, cumulus-oocyte complexes (COCs) were maintained for 0, 10 min, 6 h or 22 h in TCM 199 medium supplemented with 20 nM wortmannin, an inhibitor of PI3K. After each period, COCs were transferred to the same medium without wortmannin and kept under the same conditions until completion of 22 h of in vitro maturation (IVM). To evaluate the effect of time on progression of nucle...
Source: Zygote - August 2, 2020 Category: Reproduction Medicine Authors: Pereira JL, Curcio AG, Barroso LM, Mogollón-Waltero EM, Gomes HF, Maia RC, Viana KS, Caldas Bussiere MC, Marin DFD, Dias AJB Tags: Zygote Source Type: research
Housekeeping genes for RT-qPCR in ovine preimplantation embryos.
In conclusion, GAPDH, TBP and H3F3A are stable HKGs in ovine parthenogenetic embryos and allow accurate RT-qPCR-based gene expression analysis. PMID: 32727630 [PubMed - as supplied by publisher] (Source: Zygote)
Source: Zygote - July 29, 2020 Category: Reproduction Medicine Authors: Nascimento PS, Moura MT, Silva RLO, Ramos-Deus P, Ferreira-Silva JC, Veira JIT, Santos Filho AS, Guido SI, Bartolomeu CC, Benko-Iseppon AM, Oliveira MAL Tags: Zygote Source Type: research
Dynamic alterations in H4K12 acetylation during meiotic maturation and after parthenogenetic activation of mouse oocytes.
In conclusion, H4K12 is deacetylated in GVBD and MII, and re-hyperacetylated after PA. PMID: 32698925 [PubMed - as supplied by publisher] (Source: Zygote)
Source: Zygote - July 22, 2020 Category: Reproduction Medicine Authors: Zhang Z, Chen B, Cui H, Gao H, Gao M, Tao C Tags: Zygote Source Type: research
Maternal ageing causes changes in DNA methylation and gene expression profiles in mouse oocytes.
Abstract Although it is well known that maternal ageing causes reduced oocyte quality and fertility, little information is known about its effect on germ cell epigenetics. In the present study, we compared the gene expression and DNA methylation profiles in germinal vesicle oocytes from young (8-week-old) and aged (18-month-old) mice using single-cell RNA-sequencing and single-cell whole-genome DNA methylation sequencing. We found significant differences in the data from the two groups. Oocytes from aged mice showed significant changes in the expression of some metabolism-related genes, such as mitochondria-associ...
Source: Zygote - July 7, 2020 Category: Reproduction Medicine Authors: Hou G, Sun QY Tags: Zygote Source Type: research