Effects of antifreeze protein from < em > Lolium perenne < /em > L. ( < em > Lp < /em > AFP) in the vitrification of < em > in vitro < /em > -produced bovine embryos
In conclusion, the addition of 500 ng/ml of LpAFP during the vitrification of in vitro-produced bovine embryos improved the hatching rate and total cell number of blastocysts after warming and mitigated intracellular damage.PMID:37366027 | DOI:10.1017/S0967199423000333 (Source: Zygote)
Source: Zygote - June 27, 2023 Category: Reproduction Medicine Authors: R A Silva J únior R Desenzi M M S Ramires A F Souza M A M Donato C A Peixoto T Nascimento C C Bartolomeu A M Batista Source Type: research
The artificial uterus: on the way to ectogenesis
Zygote. 2023 Jun 26:1-11. doi: 10.1017/S0967199423000175. Online ahead of print.ABSTRACTThe inability to support the growth and development of a mature fetus up to delivery results in significant human suffering. Current available solutions include adoption, surrogacy, and uterus transplantation. However, these options are subject to several ethical, religious, economic, social, and medical concerns. Ectogenesis is the process in which an embryo develops in an artificial uterus from implantation through to the delivery of a live infant. This current narrative review summarizes the state of recent research focused on human ...
Source: Zygote - June 26, 2023 Category: Reproduction Medicine Authors: Francesco Maria Bulletti Romualdo Sciorio Antonio Palagiano Carlo Bulletti Source Type: research
Alterations in the expression pattern of some epigenetic-related genes and microRNAs subsequent to oocyte cryopreservation
Zygote. 2023 Jun 20:1-9. doi: 10.1017/S0967199423000321. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes an...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Ali Shadmanesh Hassan Nazari Source Type: research
Protective effect of onion peel extract on ageing mouse oocytes
In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in vitro in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of SOD1, CAT, and GPX3 genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Yong-Nan Xu Guo-Bo Han Ying-Hua Li Chun-Hao Piao Guan-Hao Li Nam-Hyung Kim Source Type: research
Alterations in the expression pattern of some epigenetic-related genes and microRNAs subsequent to oocyte cryopreservation
Zygote. 2023 Jun 20:1-9. doi: 10.1017/S0967199423000321. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes an...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Ali Shadmanesh Hassan Nazari Source Type: research
Protective effect of onion peel extract on ageing mouse oocytes
In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in vitro in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of SOD1, CAT, and GPX3 genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Yong-Nan Xu Guo-Bo Han Ying-Hua Li Chun-Hao Piao Guan-Hao Li Nam-Hyung Kim Source Type: research
Alterations in the expression pattern of some epigenetic-related genes and microRNAs subsequent to oocyte cryopreservation
Zygote. 2023 Jun 20:1-9. doi: 10.1017/S0967199423000321. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes an...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Ali Shadmanesh Hassan Nazari Source Type: research
Protective effect of onion peel extract on ageing mouse oocytes
In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in vitro in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of SOD1, CAT, and GPX3 genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Yong-Nan Xu Guo-Bo Han Ying-Hua Li Chun-Hao Piao Guan-Hao Li Nam-Hyung Kim Source Type: research
Alterations in the expression pattern of some epigenetic-related genes and microRNAs subsequent to oocyte cryopreservation
Zygote. 2023 Jun 20:1-9. doi: 10.1017/S0967199423000321. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes an...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Ali Shadmanesh Hassan Nazari Source Type: research
Protective effect of onion peel extract on ageing mouse oocytes
In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in vitro in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of SOD1, CAT, and GPX3 genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Yong-Nan Xu Guo-Bo Han Ying-Hua Li Chun-Hao Piao Guan-Hao Li Nam-Hyung Kim Source Type: research
Alterations in the expression pattern of some epigenetic-related genes and microRNAs subsequent to oocyte cryopreservation
Zygote. 2023 Jun 20:1-9. doi: 10.1017/S0967199423000321. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes an...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Ali Shadmanesh Hassan Nazari Source Type: research
Protective effect of onion peel extract on ageing mouse oocytes
In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in vitro in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of SOD1, CAT, and GPX3 genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly...
Source: Zygote - June 20, 2023 Category: Reproduction Medicine Authors: Yong-Nan Xu Guo-Bo Han Ying-Hua Li Chun-Hao Piao Guan-Hao Li Nam-Hyung Kim Source Type: research
Liquid storage of porcine < em > in vitro < /em > -produced blastocysts; a practical approach for short storage
In conclusion, in vitro-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO2-independent medium without compromising quality.PMID:37288532 | DOI:10.1017/S0967199423000308 (Source: Zygote)
Source: Zygote - June 8, 2023 Category: Reproduction Medicine Authors: Linda Marijke Haug Reina Jochems Ann Helen Gaustad Elisabeth Kommisrud Fr øydis Deinboll Myromslien Eli Grindflek Anne Hege Alm-Kristiansen Source Type: research
Liquid storage of porcine < em > in vitro < /em > -produced blastocysts; a practical approach for short storage
In conclusion, in vitro-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO2-independent medium without compromising quality.PMID:37288532 | DOI:10.1017/S0967199423000308 (Source: Zygote)
Source: Zygote - June 8, 2023 Category: Reproduction Medicine Authors: Linda Marijke Haug Reina Jochems Ann Helen Gaustad Elisabeth Kommisrud Fr øydis Deinboll Myromslien Eli Grindflek Anne Hege Alm-Kristiansen Source Type: research
Liquid storage of porcine < em > in vitro < /em > -produced blastocysts; a practical approach for short storage
In conclusion, in vitro-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO2-independent medium without compromising quality.PMID:37288532 | DOI:10.1017/S0967199423000308 (Source: Zygote)
Source: Zygote - June 8, 2023 Category: Reproduction Medicine Authors: Linda Marijke Haug Reina Jochems Ann Helen Gaustad Elisabeth Kommisrud Fr øydis Deinboll Myromslien Eli Grindflek Anne Hege Alm-Kristiansen Source Type: research
