CRMP5 participates in oocyte meiosis by regulating spastin to correct microtubule-kinetochore misconnection
In this study, we found that, after knocking down CRMP5 in oocytes, spastin distribution shifted from the spindle to the spindle poles and errors in microtubule-kinetochore attachment appeared in oocyte spindles. However, CRMP5 did not interact with the other two microtubule-severing proteins, katanin-like-1 (KATNAL1) and fidgetin-like-1 (FIGNL1), which aggregate at the spindle poles. We speculate that, in oocytes, due to the reduction of spastin distribution on chromosomes after knocking down CRMP5, microtubule-kinetochore errors cannot be corrected through severing, resulting in meiotic division abnormalities and maturat...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Zhen Jin Zhi-Cai Zhang Chen-Yu Xiao Mei-Qi Li Qian-Ru Li Lei-Lei Gao Source Type: research
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development
Zygote. 2023 Dec 4:1-10. doi: 10.1017/S0967199423000576. Online ahead of print.ABSTRACTOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus-oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspas...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Paola Maria da Silva Rosa Pedro Henrique Evagelista Guedes Joaquim Mansano Garcia Clara Slade Oliveira Source Type: research
The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during < em > in vitro < /em > culture
Zygote. 2023 Dec 4:1-7. doi: 10.1017/S0967199423000461. Online ahead of print.ABSTRACTThe aim of this research was to investigate the effect of Coenzyme Q10 (CoQ10) on the expression of the Transcription Factor A Mitochondrial (Tfam) gene and mtDNA copy number in preantral follicles (PFs) of mice during in vitro culture. To conduct this experimental study, PFs were isolated from 14-day-old National Medical Research Institute mice and cultured in the presence of 50 µm CoQ10 for 12 days. On the 12th day, human chorionic gonadotropin was added to stimulate ovulation. The fundamental parameters, including preantral follicle d...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Roya Harsini Saeed Zavareh Meysam Nasiri Sara Seyfi Source Type: research
CRMP5 participates in oocyte meiosis by regulating spastin to correct microtubule-kinetochore misconnection
In this study, we found that, after knocking down CRMP5 in oocytes, spastin distribution shifted from the spindle to the spindle poles and errors in microtubule-kinetochore attachment appeared in oocyte spindles. However, CRMP5 did not interact with the other two microtubule-severing proteins, katanin-like-1 (KATNAL1) and fidgetin-like-1 (FIGNL1), which aggregate at the spindle poles. We speculate that, in oocytes, due to the reduction of spastin distribution on chromosomes after knocking down CRMP5, microtubule-kinetochore errors cannot be corrected through severing, resulting in meiotic division abnormalities and maturat...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Zhen Jin Zhi-Cai Zhang Chen-Yu Xiao Mei-Qi Li Qian-Ru Li Lei-Lei Gao Source Type: research
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development
Zygote. 2023 Dec 4:1-10. doi: 10.1017/S0967199423000576. Online ahead of print.ABSTRACTOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus-oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspas...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Paola Maria da Silva Rosa Pedro Henrique Evagelista Guedes Joaquim Mansano Garcia Clara Slade Oliveira Source Type: research
The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during < em > in vitro < /em > culture
Zygote. 2023 Dec 4:1-7. doi: 10.1017/S0967199423000461. Online ahead of print.ABSTRACTThe aim of this research was to investigate the effect of Coenzyme Q10 (CoQ10) on the expression of the Transcription Factor A Mitochondrial (Tfam) gene and mtDNA copy number in preantral follicles (PFs) of mice during in vitro culture. To conduct this experimental study, PFs were isolated from 14-day-old National Medical Research Institute mice and cultured in the presence of 50 µm CoQ10 for 12 days. On the 12th day, human chorionic gonadotropin was added to stimulate ovulation. The fundamental parameters, including preantral follicle d...
Source: Zygote - December 4, 2023 Category: Reproduction Medicine Authors: Roya Harsini Saeed Zavareh Meysam Nasiri Sara Seyfi Source Type: research
Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos
In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) ma...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Bin Meng Jiahui He Wenbin Cao Yanru Zhang Jia Qi Shiwei Luo Chong Shen Juan Zhao Ying Xue Pengxiang Qu Enqi Liu Source Type: research
Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment
Zygote. 2023 Nov 29:1-7. doi: 10.1017/S0967199423000515. Online ahead of print.ABSTRACTAbnormalities in the zona pellucida (ZP) adversely affect oocyte maturation, embryo development and pregnancy outcomes. However, the assessment of severity is challenging. To evaluate the effects of different degrees of ZP abnormalities on embryo development and clinical outcomes, in total, 590 retrieval cycles were scored and divided into four categories (control, mild, moderate and severe) based on three parameters: perivitelline space, percentage of immature oocytes and percentage of oocytes with abnormal morphology. As the severity o...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Junshun Fang Hua Sun Linjun Chen Jie Wang Fei Lin Zhipeng Xu Lihua Zhu Shanshan Wang Source Type: research
Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos
In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) ma...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Bin Meng Jiahui He Wenbin Cao Yanru Zhang Jia Qi Shiwei Luo Chong Shen Juan Zhao Ying Xue Pengxiang Qu Enqi Liu Source Type: research
Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment
Zygote. 2023 Nov 29:1-7. doi: 10.1017/S0967199423000515. Online ahead of print.ABSTRACTAbnormalities in the zona pellucida (ZP) adversely affect oocyte maturation, embryo development and pregnancy outcomes. However, the assessment of severity is challenging. To evaluate the effects of different degrees of ZP abnormalities on embryo development and clinical outcomes, in total, 590 retrieval cycles were scored and divided into four categories (control, mild, moderate and severe) based on three parameters: perivitelline space, percentage of immature oocytes and percentage of oocytes with abnormal morphology. As the severity o...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Junshun Fang Hua Sun Linjun Chen Jie Wang Fei Lin Zhipeng Xu Lihua Zhu Shanshan Wang Source Type: research
Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos
In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) ma...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Bin Meng Jiahui He Wenbin Cao Yanru Zhang Jia Qi Shiwei Luo Chong Shen Juan Zhao Ying Xue Pengxiang Qu Enqi Liu Source Type: research
Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment
Zygote. 2023 Nov 29:1-7. doi: 10.1017/S0967199423000515. Online ahead of print.ABSTRACTAbnormalities in the zona pellucida (ZP) adversely affect oocyte maturation, embryo development and pregnancy outcomes. However, the assessment of severity is challenging. To evaluate the effects of different degrees of ZP abnormalities on embryo development and clinical outcomes, in total, 590 retrieval cycles were scored and divided into four categories (control, mild, moderate and severe) based on three parameters: perivitelline space, percentage of immature oocytes and percentage of oocytes with abnormal morphology. As the severity o...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Junshun Fang Hua Sun Linjun Chen Jie Wang Fei Lin Zhipeng Xu Lihua Zhu Shanshan Wang Source Type: research
Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos
In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) ma...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Bin Meng Jiahui He Wenbin Cao Yanru Zhang Jia Qi Shiwei Luo Chong Shen Juan Zhao Ying Xue Pengxiang Qu Enqi Liu Source Type: research
Embryological characteristics and clinical outcomes of oocytes with different degrees of abnormal zona pellucida during assisted reproductive treatment
Zygote. 2023 Nov 29:1-7. doi: 10.1017/S0967199423000515. Online ahead of print.ABSTRACTAbnormalities in the zona pellucida (ZP) adversely affect oocyte maturation, embryo development and pregnancy outcomes. However, the assessment of severity is challenging. To evaluate the effects of different degrees of ZP abnormalities on embryo development and clinical outcomes, in total, 590 retrieval cycles were scored and divided into four categories (control, mild, moderate and severe) based on three parameters: perivitelline space, percentage of immature oocytes and percentage of oocytes with abnormal morphology. As the severity o...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Junshun Fang Hua Sun Linjun Chen Jie Wang Fei Lin Zhipeng Xu Lihua Zhu Shanshan Wang Source Type: research
Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos
In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) ma...
Source: Zygote - November 29, 2023 Category: Reproduction Medicine Authors: Bin Meng Jiahui He Wenbin Cao Yanru Zhang Jia Qi Shiwei Luo Chong Shen Juan Zhao Ying Xue Pengxiang Qu Enqi Liu Source Type: research
