The Co-Twin Control Design: Implementation and Methodological Considerations
This article introduces the CTCD and offers an overview of analysis methods for binary and continuous outcome and exposure variables. Tools for data simulation are provided, along with practical guidance and accompanying scripts for implementing the CTCD in R, SPSS, and Stata. While the CTCD offers valuable insights into causal inference, it depends on several assumptions that are important when interpreting CTCD results. By presenting a broad overview of the CTCD, this article aims to equip researchers with actionable recommendations and a comprehensive understanding of the design's strengths and limitations.PMID:37655521...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Bodine M A Gonggrijp Steve G A van de Weijer Catrien C J H Bijleveld Jenny van Dongen Dorret I Boomsma Source Type: research
Mitochondrial DNA Copy Number and Heteroplasmy in Monozygotic Twins Discordant for Schizophrenia
This report specifically focuses on the assessment of changes in the mitochondrial genome in a unique set of monozygotic twins discordant (MZD) for SZ using blood. Genomic DNA from six pairs of MZD twins and two sets of parents (N = 16) was hybridized to the Affymetrix Human SNP Array 6.0 to assess mitochondrial DNA copy number (mtDNA-CN). Whole genome sequencing (WGS) and quantitative polymerase chain reaction (qPCR) was performed for a subset of MZD pairs and their parents and was also used to derive mtDNA-CN estimates. The WGS data were further analyzed to generate heteroplasmy (HP) estimates. Our results show that mtDN...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Phyo W Win Shiva M Singh Christina A Castellani Source Type: research
Early Spontaneous Twinning Recorded By Time-Lapse
We present the complete TL record of one case of MZT that split itself at the very moment of the division into two cells, with one of the cells coming out through a hole in the zona pellucida (ZP). Both resulting embryos developed normally, and were vitrified. It is suggested that the hole in the ZP may facilitate the extrusion of some cells of the <day 4 embryo and that this cell development is not constrained by being inside the ZP. Despite the lack of the inhibition of the ZP itself or the influence of the other embryo cells, the totipotent cell was then able to develop correctly from the start. Moreover, the embryo ...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Roberto Matorras Alberto Vendrell Marcos Ferrando Zaloa Larreategui Source Type: research
The Co-Twin Control Design: Implementation and Methodological Considerations
This article introduces the CTCD and offers an overview of analysis methods for binary and continuous outcome and exposure variables. Tools for data simulation are provided, along with practical guidance and accompanying scripts for implementing the CTCD in R, SPSS, and Stata. While the CTCD offers valuable insights into causal inference, it depends on several assumptions that are important when interpreting CTCD results. By presenting a broad overview of the CTCD, this article aims to equip researchers with actionable recommendations and a comprehensive understanding of the design's strengths and limitations.PMID:37655521...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Bodine M A Gonggrijp Steve G A van de Weijer Catrien C J H Bijleveld Jenny van Dongen Dorret I Boomsma Source Type: research
Mitochondrial DNA Copy Number and Heteroplasmy in Monozygotic Twins Discordant for Schizophrenia
This report specifically focuses on the assessment of changes in the mitochondrial genome in a unique set of monozygotic twins discordant (MZD) for SZ using blood. Genomic DNA from six pairs of MZD twins and two sets of parents (N = 16) was hybridized to the Affymetrix Human SNP Array 6.0 to assess mitochondrial DNA copy number (mtDNA-CN). Whole genome sequencing (WGS) and quantitative polymerase chain reaction (qPCR) was performed for a subset of MZD pairs and their parents and was also used to derive mtDNA-CN estimates. The WGS data were further analyzed to generate heteroplasmy (HP) estimates. Our results show that mtDN...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Phyo W Win Shiva M Singh Christina A Castellani Source Type: research
Early Spontaneous Twinning Recorded By Time-Lapse
We present the complete TL record of one case of MZT that split itself at the very moment of the division into two cells, with one of the cells coming out through a hole in the zona pellucida (ZP). Both resulting embryos developed normally, and were vitrified. It is suggested that the hole in the ZP may facilitate the extrusion of some cells of the <day 4 embryo and that this cell development is not constrained by being inside the ZP. Despite the lack of the inhibition of the ZP itself or the influence of the other embryo cells, the totipotent cell was then able to develop correctly from the start. Moreover, the embryo ...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Roberto Matorras Alberto Vendrell Marcos Ferrando Zaloa Larreategui Source Type: research
The Co-Twin Control Design: Implementation and Methodological Considerations
This article introduces the CTCD and offers an overview of analysis methods for binary and continuous outcome and exposure variables. Tools for data simulation are provided, along with practical guidance and accompanying scripts for implementing the CTCD in R, SPSS, and Stata. While the CTCD offers valuable insights into causal inference, it depends on several assumptions that are important when interpreting CTCD results. By presenting a broad overview of the CTCD, this article aims to equip researchers with actionable recommendations and a comprehensive understanding of the design's strengths and limitations.PMID:37655521...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Bodine M A Gonggrijp Steve G A van de Weijer Catrien C J H Bijleveld Jenny van Dongen Dorret I Boomsma Source Type: research
Mitochondrial DNA Copy Number and Heteroplasmy in Monozygotic Twins Discordant for Schizophrenia
This report specifically focuses on the assessment of changes in the mitochondrial genome in a unique set of monozygotic twins discordant (MZD) for SZ using blood. Genomic DNA from six pairs of MZD twins and two sets of parents (N = 16) was hybridized to the Affymetrix Human SNP Array 6.0 to assess mitochondrial DNA copy number (mtDNA-CN). Whole genome sequencing (WGS) and quantitative polymerase chain reaction (qPCR) was performed for a subset of MZD pairs and their parents and was also used to derive mtDNA-CN estimates. The WGS data were further analyzed to generate heteroplasmy (HP) estimates. Our results show that mtDN...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Phyo W Win Shiva M Singh Christina A Castellani Source Type: research
Early Spontaneous Twinning Recorded By Time-Lapse
We present the complete TL record of one case of MZT that split itself at the very moment of the division into two cells, with one of the cells coming out through a hole in the zona pellucida (ZP). Both resulting embryos developed normally, and were vitrified. It is suggested that the hole in the ZP may facilitate the extrusion of some cells of the <day 4 embryo and that this cell development is not constrained by being inside the ZP. Despite the lack of the inhibition of the ZP itself or the influence of the other embryo cells, the totipotent cell was then able to develop correctly from the start. Moreover, the embryo ...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Roberto Matorras Alberto Vendrell Marcos Ferrando Zaloa Larreategui Source Type: research
The Co-Twin Control Design: Implementation and Methodological Considerations
This article introduces the CTCD and offers an overview of analysis methods for binary and continuous outcome and exposure variables. Tools for data simulation are provided, along with practical guidance and accompanying scripts for implementing the CTCD in R, SPSS, and Stata. While the CTCD offers valuable insights into causal inference, it depends on several assumptions that are important when interpreting CTCD results. By presenting a broad overview of the CTCD, this article aims to equip researchers with actionable recommendations and a comprehensive understanding of the design's strengths and limitations.PMID:37655521...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Bodine M A Gonggrijp Steve G A van de Weijer Catrien C J H Bijleveld Jenny van Dongen Dorret I Boomsma Source Type: research
Mitochondrial DNA Copy Number and Heteroplasmy in Monozygotic Twins Discordant for Schizophrenia
This report specifically focuses on the assessment of changes in the mitochondrial genome in a unique set of monozygotic twins discordant (MZD) for SZ using blood. Genomic DNA from six pairs of MZD twins and two sets of parents (N = 16) was hybridized to the Affymetrix Human SNP Array 6.0 to assess mitochondrial DNA copy number (mtDNA-CN). Whole genome sequencing (WGS) and quantitative polymerase chain reaction (qPCR) was performed for a subset of MZD pairs and their parents and was also used to derive mtDNA-CN estimates. The WGS data were further analyzed to generate heteroplasmy (HP) estimates. Our results show that mtDN...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Phyo W Win Shiva M Singh Christina A Castellani Source Type: research
Early Spontaneous Twinning Recorded By Time-Lapse
We present the complete TL record of one case of MZT that split itself at the very moment of the division into two cells, with one of the cells coming out through a hole in the zona pellucida (ZP). Both resulting embryos developed normally, and were vitrified. It is suggested that the hole in the ZP may facilitate the extrusion of some cells of the <day 4 embryo and that this cell development is not constrained by being inside the ZP. Despite the lack of the inhibition of the ZP itself or the influence of the other embryo cells, the totipotent cell was then able to develop correctly from the start. Moreover, the embryo ...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Roberto Matorras Alberto Vendrell Marcos Ferrando Zaloa Larreategui Source Type: research
The Co-Twin Control Design: Implementation and Methodological Considerations
This article introduces the CTCD and offers an overview of analysis methods for binary and continuous outcome and exposure variables. Tools for data simulation are provided, along with practical guidance and accompanying scripts for implementing the CTCD in R, SPSS, and Stata. While the CTCD offers valuable insights into causal inference, it depends on several assumptions that are important when interpreting CTCD results. By presenting a broad overview of the CTCD, this article aims to equip researchers with actionable recommendations and a comprehensive understanding of the design's strengths and limitations.PMID:37655521...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Bodine M A Gonggrijp Steve G A van de Weijer Catrien C J H Bijleveld Jenny van Dongen Dorret I Boomsma Source Type: research
Mitochondrial DNA Copy Number and Heteroplasmy in Monozygotic Twins Discordant for Schizophrenia
This report specifically focuses on the assessment of changes in the mitochondrial genome in a unique set of monozygotic twins discordant (MZD) for SZ using blood. Genomic DNA from six pairs of MZD twins and two sets of parents (N = 16) was hybridized to the Affymetrix Human SNP Array 6.0 to assess mitochondrial DNA copy number (mtDNA-CN). Whole genome sequencing (WGS) and quantitative polymerase chain reaction (qPCR) was performed for a subset of MZD pairs and their parents and was also used to derive mtDNA-CN estimates. The WGS data were further analyzed to generate heteroplasmy (HP) estimates. Our results show that mtDN...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Phyo W Win Shiva M Singh Christina A Castellani Source Type: research
Early Spontaneous Twinning Recorded By Time-Lapse
We present the complete TL record of one case of MZT that split itself at the very moment of the division into two cells, with one of the cells coming out through a hole in the zona pellucida (ZP). Both resulting embryos developed normally, and were vitrified. It is suggested that the hole in the ZP may facilitate the extrusion of some cells of the <day 4 embryo and that this cell development is not constrained by being inside the ZP. Despite the lack of the inhibition of the ZP itself or the influence of the other embryo cells, the totipotent cell was then able to develop correctly from the start. Moreover, the embryo ...
Source: Twin Research and Human Genetics - September 1, 2023 Category: Genetics & Stem Cells Authors: Roberto Matorras Alberto Vendrell Marcos Ferrando Zaloa Larreategui Source Type: research
