Surface-mediated fluorescent sensor array for identification of gut microbiota and monitoring of colorectal cancer
Talanta. 2024 Apr 8;274:126081. doi: 10.1016/j.talanta.2024.126081. Online ahead of print.ABSTRACTThe development of efficient, accurate, and high-throughput technology for gut microbiota sensing holds great promise in the maintenance of health and the treatment of diseases. Herein, we developed a rapid fluorescent sensor array based on surface-engineered silver nanoparticles (AgNPs) and vancomycin-modified gold nanoclusters (AuNCs@Van) for gut microbiota sensing. By controlling the surface of AgNPs, the recognition ability of the sensor can be effectively improved. The sensor array was used to successfully discriminate si...
Source: Talanta - April 13, 2024 Category: Chemistry Authors: Zhihui Liu Meizi Zeng Yuquan Xiao Xiaohua Zhu Meiling Liu Ying Long Haitao Li Youyu Zhang Shouzhuo Yao Source Type: research
Design of a prodrug photocage for cancer cells detection and anticancer drug release
This article presents the design and synthesis of a novel Rhodamine B fluorescence probe. The design strategy involves utilizing an anticancer drug (Melphalan) to bind with a fluorescent group (HRhod-OH), forming HRhod-MeL, which is non-fluorescent. However, when exposed to the high levels of reactive oxygen species (ROS) of cancer cells, HRhod-MeL transforms into a red-emitting Photocage (Rhod-MeL), and selectively accumulates in the mitochondria of cancer cells, where, when activated with green light (556 nm), anti-cancer drugs released. The Photocage improve the efficacy of anti-cancer drugs and enables the precise diag...
Source: Talanta - April 13, 2024 Category: Chemistry Authors: Qianshan Shao Fei Zhang Chunxiao Li Yuyu Yang Shihan Liu Guang Chen Baolei Fan Source Type: research
Advances on fluorescence chemosensors for selective detection of water
Talanta. 2024 Apr 9;275:126089. doi: 10.1016/j.talanta.2024.126089. Online ahead of print.ABSTRACTWater, although an important part of everyday life, is acts as one of the most significant contaminants in various applications such as biomedical monitoring, chemical production, petroleum-based fuel and food processing. In fact, the presence of water in other solvents is a huge concern. For the quantification of trace water content, different methods such as Karl-Fischer, electrochemical, nuclear magnetic resonance, chromatography, and thermogravimetric analysis have been used. Although every technique has its own benefit, e...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Pragyan Parimita Dash Arup Kumar Ghosh Patitapaban Mohanty Rubi Behura Sunita Behera Bigyan R Jali Suban K Sahoo Source Type: research
A new derivatizing reagent for the determination of 5-nitro-2-furaldehyde in trout muscle by liquid chromatography-tandem mass spectrometry
This study aims at developping an analytical method for 5-NF determination in trout muscle samples based on chemical derivatization prior to analysis by liquid chromatography-tandem mass spectrometry. Four commercially available hydrazine reagents, namely: N,N-Dimethylhydrazine (DMH), 4-Hydrazinobenzoic acid (HBA), 2,4-Dichlorophenylhydrazine (2,4-DCPH) and 2,6-Dichlorophenylhydrazine (2,6-DCPH) were proposed for the first time as derivatizing reagents in the analysis of 5-NF. The derivatization reaction was simultaneously performed along with the extraction method in acidic condition using ultrasonic assistance and follow...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Khaled El Hawari Dominique Hurtaud-Pessel Eric Verdon Source Type: research
Rapid screening of infertility-associated gynecological conditions via ambient glow discharge mass spectrometry utilizing urine metabolic fingerprints
Talanta. 2024 Apr 8;274:125969. doi: 10.1016/j.talanta.2024.125969. Online ahead of print.ABSTRACTInfertility presents a widespread challenge for many families worldwide, often arising from various gynecological diseases (GDs) that hinder successful pregnancies. Current diagnostic methods for GDs have disadvantages such as low efficiency, high cost, misdiagnose, invasive injury and etc. This paper introduces a rapid, non-invasive, efficient, and straightforward analytical method that utilizes desorption, separation, and ionization mass spectrometry (DSI-MS) platform in conjunction with machine learning (ML) to detect urine...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Yijiao Qu Ming Chen Yiran Wang Liangliang Qu Ruiyue Wang Huihui Liu Liping Wang Zongxiu Nie Source Type: research
A strategy to enhance the water solubility of luminescent β-diketonate-Europium(III) complexes for time-gated luminescence bioassays
Talanta. 2024 Mar 27;274:126000. doi: 10.1016/j.talanta.2024.126000. Online ahead of print.ABSTRACTLuminescent β-diketonate-europium(III) complexes have been found a wide range of applications in time-gated luminescence (TGL) bioassays, but their poor water solubility is a main problem that limits their effective uses. In this work we propose a simple and general strategy to enhance the water solubility of luminescent β-diketonate-europium(III) complexes that permits facile synthesis and purification. By introducing the fluorinated carboxylic acid group into the structures of β-diketone ligands, two highly water-soluble...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Zhiyuan Dong Bo Song Hua Ma Xiaona Gao Wenzhu Zhang Jingli Yuan Source Type: research
Direct electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of matrix metalloproteinase 2
Talanta. 2024 Apr 9;274:126079. doi: 10.1016/j.talanta.2024.126079. Online ahead of print.ABSTRACTSimple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Stefan Jari ć Silvia Schobesberger Lazar Velicki Aleksandra Milovan čev Stanislava Nikoli ć Peter Ertl Ivan Bobrinetskiy Nikola Ž Knežević Source Type: research
Direct detection of acetylcholinesterase by Fe(HCOO) < sub > 2.6 < /sub > (OH) < sub > 0.3 < /sub > . H < sub > 2 < /sub > O nanosheets with oxidase-like activity on a smartphone platform
Talanta. 2024 Apr 8;274:126074. doi: 10.1016/j.talanta.2024.126074. Online ahead of print.ABSTRACTMonitoring acetylcholinesterase (AChE) is crucial in clinical diagnosis and drug screening. Traditional methods for detecting AChE usually require the addition of intermediates like acetylthiocholine, which complicates the detection process and introduces interference risks. Herein, we develop a direct colorimetric assay based on alkaline iron formate nanosheets (Fe(HCOO)2.6(OH)0.3·H2O NSs, Fef NSs) for the detection of AChE without any intermediates. The as-prepared Fef NSs exhibit oxidase-like activity, catalyzing the gener...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Yongbin Cao Yuanyuan Chen Yue Zhou Xiaohua Chen Jian Peng Source Type: research
Evaluation of miR-155 silencing using a molecular beacon in human lung adenocarcinoma cell line
Talanta. 2024 Apr 7;274:126052. doi: 10.1016/j.talanta.2024.126052. Online ahead of print.ABSTRACTLung cancer (LC) is a leading cause of global cancer-related deaths, highlighting the development of innovative methods for biomarker detection improving the early diagnostics. microRNAs (miRs) alterations are known to be involved in the initiation and progression of human cancers and can act as biomarkers for diagnostics and treatment. Herein, we develop the application of molecular beacon (MB) technology to monitor miR-155-3p expression in human lung adenocarcinoma A549 cells without complementary DNA synthesis, amplificatio...
Source: Talanta - April 12, 2024 Category: Chemistry Authors: Daniela Alexandre Alexandra R Fernandes Pedro V Baptista Carla Cruz Source Type: research
A highly sensitive, long-time stable Ag/AgCl ultra-micro sensor for in situ monitoring chloride ions inside the crevice using SECM
Talanta. 2024 Apr 4;274:126026. doi: 10.1016/j.talanta.2024.126026. Online ahead of print.ABSTRACTTracking the variation of Cl- timely within the crevice is of great significance for comprehending the dynamic mechanism of crevice corrosion. The reported chloride ion selective electrodes are difficult to realize the long-time Cl- detection inside the confined crevice, due to their millimeter size or a relative limited lifespan. For this purpose, an Ag/AgCl ultra-micro sensor (UMS) with a radius of 12.5 μm was fabricated and optimized using laser drawing and electrodeposition techniques. Results show the AgCl film's structu...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Hang Zhang Hao-Ran Yuan Ze-Jie Zhu Qin-Hao Zhang Yi-Han Bai Jiang-Ying Wang Fa-He Cao Source Type: research
Dual amplification for PEC ultrasensitive aptasensing of biomarker HER-2 based on Z-scheme UiO-66/CdIn < sub > 2 < /sub > S < sub > 4 < /sub > heterojunction and flower-like PtPdCu nanozyme
Talanta. 2024 Apr 3;274:126034. doi: 10.1016/j.talanta.2024.126034. Online ahead of print.ABSTRACTAs an important prognostic indicator in breast cancer, human epithelial growth factor receptor-2 (HER-2) is of importance for assessing prognosis of breast cancer patients, whose accurate and facile analysis are imperative in clinical diagnosis and treatment. Herein, photoactive Z-scheme UiO-66/CdIn2S4 heterojunction was constructed by a hydrothermal method, whose optical property and photoactivity were critically investigated by a range of techniques, combined by elucidating the interfacial charge transfer mechanism. Meanwhil...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Qing-Ying Ai Ben-Fang Xu Fan Xu Ai-Jun Wang Li-Ping Mei Liang Wu Pei Song Jiu-Ju Feng Source Type: research
Portable one-step effervescence tablet-based microextraction combined with smartphone digital image colorimetry: Toward field and rapid detection of trace nickel ion
In this study, the one-step switchable hydrophilic solvent (SHS)-based effervescence tablet microextraction (ETME) was coupled with smartphone digital image colorimetry (SDIC) for the field detection of nickel ion (Ni2+) for the first time. Both extractant and CO2 were generated in situ when the novel SHS-based effervescence tablet was placed in the sample solution. The complexant 1-(2-pyridinylazo)-2-naphthaleno (PAN) dissolved from the effervescence tablet to form a stable complex with Ni2+, and the extractant was uniformly dispersed in the sample solution under the action of CO2 and fully in contact with Ni-PAN, which e...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Yiping Sun Xiaofang Yang Jiayi Hu Fuchun Ji Huajian Chi Ya Liu Kan Hu Fangfang Hao Xiaodong Wen Source Type: research
RuZn NPs with electroactivity and oxidase-like property for dual-mode anti-cancer drug monitoring
Talanta. 2024 Apr 8;274:126075. doi: 10.1016/j.talanta.2024.126075. Online ahead of print.ABSTRACT6-mercaptopurine (6-MP) as the effective anti-cancer drug was used for the treatment of Crohn's disease and acute lymphoblastic leukaemia, but the response to maintenance therapy was variable with individual differences. In order to control the dosage and decrease the side effects of 6-MP, a sensitive and stable assay was urgently needed for 6-MP monitoring. Herein, RuZn NPs with electrochemical oxidation property and oxidase-like activity was proposed for dual-mode 6-MP monitoring. Burr-like RuZn NPs were prepared and explore...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Zhao Jin Fang Peng Qiaodan Du Dan Liang Yuan Zhao Source Type: research
Smartphone-based dual inverse signal MOFs fluorescence sensing for intelligent on-site visual detection of malachite green
Talanta. 2024 Apr 4;274:126039. doi: 10.1016/j.talanta.2024.126039. Online ahead of print.ABSTRACTThe development of intelligent, sensitive, and visual methods for the rapid detection of veterinary drug residues is essential to ensure food quality and safety. Here, a smartphone-based dual inverse signal MOFs fluorescence sensing system was proposed for intelligent in-site visual detection of malachite green (MG). A UiO-66-NH2@RhB-dual-emission fluorescent probe was successfully synthesized in one step using a simple one-pot method. The inner filter effect (IFE) quenches the red fluorescence, while hydrogen bonding interact...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Lianshuang Jiang Chenghui Li Xiandeng Hou Source Type: research
Design, synthesis, and evaluation of a carboxylesterase detection probe with therapeutic effects
In this study, a lysosomal targeting fluorescent probe recognition on CEs was designed and synthesized. The obtained probe BF2-cur-Mor demonstrated excellent selectivity, sensitivity, pH-independence, and enzyme affinity towards CEs within 5 min. BF2-cur-Mor could enable recognition of intracellular CEs and elucidate that the CEs content of different cancer cells follows the rule of HepG2 > HCT-116 > A549 > HeLa, and the CEs expression level of hepatoma cancer cells far exceeds that of normal hepatic cells, being in good agreement with the previous reports. The ability of BF2-cur-Mor to monitor CEs in vivo was con...
Source: Talanta - April 11, 2024 Category: Chemistry Authors: Xia Lin Min Liu Qingyuan Yi Ying Zhou Jinchan Su Binyang Qing Yaqi Lu Chunxiao Pu Weisen Lan Lianjia Zou Jianyi Wang Source Type: research
