Design of an effective small expression tag to enhance GPCR production in E. coli ‐based cell‐free and whole cell expression systems
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Source: Protein Science - November 16, 2023 Category: Biochemistry Authors: Seongyeon Cho, Haein Lee, Yong Hee Han, Tae Shin Park, Sang Woo Seo, Tai Hyun Park Tags: RESEARCH ARTICLE Source Type: research

Nanoscale Structural Characterization of Transthyretin Aggregates Formed at Different Time Points of Protein Aggregation Using Atomic Force Microscopy ‐Infrared Spectroscopy
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Source: Protein Science - November 15, 2023 Category: Biochemistry Authors: Axell Rodriguez, Abid Ali, Aidan P. Holman, Tianyi Dou, Kiryl Zhaliazka, Dmitry Kurouski Tags: RESEARCH ARTICLE Source Type: research

SSDraw: software for generating comparative protein secondary structure diagrams
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Source: Protein Science - November 13, 2023 Category: Biochemistry Authors: Ethan A. Chen, Lauren L. Porter Tags: TOOLS FOR PROTEIN SCIENCE Source Type: research

Engineered Antigen ‐Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes
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Source: Protein Science - November 10, 2023 Category: Biochemistry Authors: Heather A. Bruce, Alexander U. Singer, Ekaterina V. Filippova, Levi L. Blazer, Jarrett J. Adams, Leonie Enderle, Moshe Ben ‐David, Elizabeth H. Radley, Daniel Y. L. Mao, Victor Pau, Stephen Orlicky, Frank Sicheri, Igor Kourinov, Shane Atwel Tags: RESEARCH ARTICLE Source Type: research

Widespread amyloid aggregates formation by Zika virus proteins and peptides
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Source: Protein Science - November 9, 2023 Category: Biochemistry Authors: Rajanish Giri, Taniya Bhardwaj, Shivani K. Kapuganti, Kumar Udit Saumya, Nitin Sharma, Aparna Bhardwaj, Richa Joshi, Deepanshu Verma, Kundlik Gadhave Tags: RESEARCH ARTICLE Source Type: research

Only kosmotrope anions trigger fibrillization of the recombinant core spidroin eADF4(C16) from Araneus diadematus
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Source: Protein Science - November 8, 2023 Category: Biochemistry Authors: Veronika Hovanov á, Andrej Hovan, Martin Humenik, Erik Sedlák Tags: RESEARCH ARTICLE Source Type: research

Enhancing Thermal Stability in the CH2 Domain to Suppress Aggregation through the Introduction of Simultaneous Disulfide Bonds in Pichia pastoris
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Source: Protein Science - November 5, 2023 Category: Biochemistry Authors: Kosuke Oyama, Makoto Nakakido, Takatoshi Ohkuri, Hitomi Nakamura, Kouhei Tsumoto, Tadashi Ueda Tags: RESEARCH ARTICLE Source Type: research

From Haemadin to Haemanorm: Synthesis and Characterization of Full ‐length Haemadin from the leech Haemadipsa sylvestris and of a Novel Bivalent, Highly Potent Thrombin Inhibitor (Haemanorm)
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Source: Protein Science - November 4, 2023 Category: Biochemistry Authors: Laura Acquasaliente, Andrea Pierangelini, Anna Pagotto, Nicola Pozzi, Vincenzo De Filippis Tags: RESEARCH ARTICLE Source Type: research

Synthesis and degradation of the cyclic dinucleotide messenger c ‐di‐AMP in the hyperthermophilic archaeon Pyrococcus yayanosii
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Source: Protein Science - November 4, 2023 Category: Biochemistry Authors: Zheng Jin, Dong Song, Wei ‐Wei Wang, Lei Feng, Zheng‐Xin Li, Hai‐Feng Chen, Xiang Xiao, Xi‐Peng Liu Tags: RESEARCH ARTICLE Source Type: research

Integrative modeling of guanylate binding protein dimers
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Source: Protein Science - November 3, 2023 Category: Biochemistry Authors: Wibke Schumann, Jennifer Loschwitz, Jens Reiners, Daniel Degrandi, Larissa Legewie, Kai St ühler, Klaus Pfeffer, Gereon Poschmann, Sander H. J. Smits, Birgit Strodel Tags: RESEARCH ARTICLE Source Type: research

Lectin ‐anticancer peptide fusion demonstrates significant cancer‐cell‐selective cytotoxic effect and inspires the production of ‘clickable’ anticancer peptide in E. coli
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Source: Protein Science - November 2, 2023 Category: Biochemistry Authors: Rajeev Pasupuleti, Sabrina Riedl, Laia Saltor N úñez, Marianna Karava, Vajinder Kumar, Robert Kourist, W. Bruce Turnbull, Dagmar Zweytick, Birgit Wiltschi Tags: RESEARCH ARTICLE Source Type: research

Complexation and evolution of cis ‐prenyltransferase homologues in Cinnamomum kanehirae deduced from kinetic and functional characterizations
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Source: Protein Science - November 2, 2023 Category: Biochemistry Authors: Jia ‐Jin Liu, Po‐Huang Liang Tags: RESEARCH ARTICLE Source Type: research

Conformational features and interaction mechanisms of VHH antibodies with β‐hairpin CDR3: A case of Nb8‐HigB2 interaction
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Source: Protein Science - November 2, 2023 Category: Biochemistry Authors: Koichi Yamamoto, Satoru Nagatoishi, Ryo Matsunaga, Makoto Nakakido, Daisuke Kuroda, Kouhei Tsumoto Tags: RESEARCH ARTICLE Source Type: research

Site ‐specific photo‐crosslinking/cleavage for protein‐protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cells
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Source: Protein Science - November 1, 2023 Category: Biochemistry Authors: Kazue Terasawa, Tatsuro Seike, Kensaku Sakamoto, Kazumasa Ohtake, Tohru Terada, Takanori Iwata, Tetsuro Watabe, Shigeyuki Yokoyama, Miki Hara ‐Yokoyama Tags: RESEARCH ARTICLE Source Type: research

Nucleocapsid proteins from human coronaviruses possess phase separation capabilities and promote FUS pathological aggregation
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Source: Protein Science - October 31, 2023 Category: Biochemistry Authors: Hui Dong, Hong Zhang, Julie Jalin, Ziqi He, Runhan Wang, Leqi Huang, Zibo Liu, Shenqing Zhang, Bin Dai, Dan Li Tags: RESEARCH ARTICLE Source Type: research