Characterizing Lysine Acetylation of Glucokinase
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 24, 2023 Category: Biochemistry Authors: Nour Fatema,
Xinyu Li,
Qinglei Gan,
Chenguang Fan Tags: RESEARCH ARTICLE Source Type: research
Molecular Interactions between Perlecan LG3 and the SARS ‐CoV‐2 spike protein RBD
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 24, 2023 Category: Biochemistry Authors: Timothy E. Gressett,
Md Lokman Hossen,
Grant Talkington,
Milla Volic,
Hugo Perez,
Purushottam B. Tiwari,
Prem Chapagain,
Gregory Bix Tags: RESEARCH ARTICLE Source Type: research
Exploring novel HIV ‐1 reverse transcriptase inhibitors with drug‐resistant mutants: A double mutant surprise
AbstractHIV-1 reverse transcriptase (RT) remains a key target for HIV drug development. As successful management of the disease requires lifelong treatment, the emergence of resistance mutations is inevitable, making development of new RT inhibitors, which remain effective against resistant variants crucial. To this end, previous computationally guided drug design efforts have resulted in catechol diether compounds, which inhibit wildtype RT with picomolar affinities and appear to be promising preclinical candidates. To confirm that these compounds remain potent against Y181C, a widespread mutation conferring resistance to...
Source: Protein Science - November 21, 2023 Category: Biochemistry Authors: Klarissa Hollander,
Albert H. Chan,
Kathleen M. Frey,
Olivia Hunker,
Joseph A. Ippolito,
Krasimir A. Spasov,
Yang ‐Hui J. Yeh,
William L. Jorgensen,
Ya‐Chi Ho,
Karen S. Anderson Tags: RESEARCH ARTICLE Source Type: research
Site ‐specific photo‐crosslinking/cleavage for protein–protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cells
AbstractGenetic code expansion enables site-specific photo-crosslinking by introducing photo-reactive non-canonical amino acids into proteins at defined positions during translation. This technology is widely used for analyzing protein –protein interactions and is applicable in mammalian cells. However, the identification of the crosslinked region still remains challenging. Here, we developed a new method to identify the crosslinked region by pre-installing a site-specific cleavage site, an α-hydroxy acid (Nε-allyloxycarbonyl- α-hydroxyl-l-lysine acid, AllocLys-OH), into the target protein. Alkaline treatment cleaves ...
Source: Protein Science - November 21, 2023 Category: Biochemistry Authors: Kazue Terasawa,
Tatsuro Seike,
Kensaku Sakamoto,
Kazumasa Ohtake,
Tohru Terada,
Takanori Iwata,
Tetsuro Watabe,
Shigeyuki Yokoyama,
Miki Hara ‐Yokoyama Tags: RESEARCH ARTICLE Source Type: research
The PB1 and the ZZ domain of the autophagy receptor p62/SQSTM1 regulate the interaction of p62/SQSTM1 with the autophagosome protein LC3B
In conclusion, our study highlights the regulated nature of p62/SQSTM1 and its ability to modulate the interaction with LC3B through a LIR-sequence Accessibility Mechanism (LAM). Furthermore, our findings suggest the potential for pharmacological modulation of the exposure of LIR, paving the way for future therapeutic strategies.This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 21, 2023 Category: Biochemistry Authors: Lucia Alcober ‐Boquet,
Tabea Zang,
Larissa Pietsch,
Evelyn Suess,
Markus Hartmann,
Ewgenij Proschak,
Lissy Z. F. Gross,
Mariana Sacerdoti,
Stefan Zeuzem,
Vladimir V. Rogov,
Alejandro E. Leroux,
Albrecht Piiper,
Ricardo M. Biondi Tags: RESEARCH ARTICLE Source Type: research
Exploring Protein ‐Protein Interactions and Oligomerization State of Pulmonary Surfactant Protein C (SP‐C) through FRET and Fluorescence Self‐Quenching
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 21, 2023 Category: Biochemistry Authors: Mishelle Mor án‐Lalangui,
Ana Coutinho,
Manuel Prieto,
Alexander Fedorov,
Jesús Pérez‐Gil,
Luís M. S. Loura,
Begoña García‐Álvarez Tags: RESEARCH ARTICLE Source Type: research
Exploring the molecular mechanism of cold ‐adaption of an alkaline protease mutant by molecular dynamics simulations and residue interaction network
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 20, 2023 Category: Biochemistry Authors: Ailan Huang,
Fuping Lu,
Fufeng Liu Tags: RESEARCH ARTICLE Source Type: research
TEPCAM: prediction of T cell receptor ‐epitope binding specificity via interpretable deep learning
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 20, 2023 Category: Biochemistry Authors: Junwei Chen,
Bowen Zhao,
Shenggeng Lin,
Heqi Sun,
Xueying Mao,
Meng Wang,
Yanyi Chu,
Liang Hong,
Dong ‐Qing Wei,
Min Li,
Yi Xiong Tags: TOOLS FOR PROTEIN SCIENCE Source Type: research
Cover Image
The cover image depicts reformulation of reverse micelles with naturally derived lipids from soybean, porcine brain, or bovine heart to develop more biologically accurate membrane models for use in high-resolution protein NMR. This model was designed for peripheral membrane proteins such as glutathione peroxidase 4 (GP ×4), as shown in the image, as well as FABP4 and PEBP1. For details, see: Walters et al., Protein Science, 2023. (Source: Protein Science)
Source: Protein Science - November 20, 2023 Category: Biochemistry Tags: Cover Image Source Type: research
Issue information
(Source: Protein Science)
Source: Protein Science - November 20, 2023 Category: Biochemistry Tags: Issue Information Source Type: research
Design of an effective small expression tag to enhance GPCR production in E. coli ‐based cell‐free and whole cell expression systems
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 16, 2023 Category: Biochemistry Authors: Seongyeon Cho,
Haein Lee,
Yong Hee Han,
Tae Shin Park,
Sang Woo Seo,
Tai Hyun Park Tags: RESEARCH ARTICLE Source Type: research
Nanoscale Structural Characterization of Transthyretin Aggregates Formed at Different Time Points of Protein Aggregation Using Atomic Force Microscopy ‐Infrared Spectroscopy
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 15, 2023 Category: Biochemistry Authors: Axell Rodriguez,
Abid Ali,
Aidan P. Holman,
Tianyi Dou,
Kiryl Zhaliazka,
Dmitry Kurouski Tags: RESEARCH ARTICLE Source Type: research
SSDraw: software for generating comparative protein secondary structure diagrams
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 13, 2023 Category: Biochemistry Authors: Ethan A. Chen,
Lauren L. Porter Tags: TOOLS FOR PROTEIN SCIENCE Source Type: research
Engineered Antigen ‐Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 10, 2023 Category: Biochemistry Authors: Heather A. Bruce,
Alexander U. Singer,
Ekaterina V. Filippova,
Levi L. Blazer,
Jarrett J. Adams,
Leonie Enderle,
Moshe Ben ‐David,
Elizabeth H. Radley,
Daniel Y. L. Mao,
Victor Pau,
Stephen Orlicky,
Frank Sicheri,
Igor Kourinov,
Shane Atwel Tags: RESEARCH ARTICLE Source Type: research
Widespread amyloid aggregates formation by Zika virus proteins and peptides
This article is protected by copyright. All rights reserved. (Source: Protein Science)
Source: Protein Science - November 9, 2023 Category: Biochemistry Authors: Rajanish Giri,
Taniya Bhardwaj,
Shivani K. Kapuganti,
Kumar Udit Saumya,
Nitin Sharma,
Aparna Bhardwaj,
Richa Joshi,
Deepanshu Verma,
Kundlik Gadhave Tags: RESEARCH ARTICLE Source Type: research
