Quantitative proteomics I.: Concept, design, and planning of quantitative proteomics experiments By Simon Sug ár, Laszlo Drahos and Karoly Vekey
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 5, 2023 Category: Chemistry Authors: Simon Sug ár,
László Drahos,
Károly Vékey Tags: COVER STORY Source Type: research
Cover Picture
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 5, 2023 Category: Chemistry Tags: COVER PICTURE Source Type: research
On ‐Tissue Chemical Derivatization of Volatile Metabolites for Matrix Assisted Laser Desorption/Ionization
AbstractMass spectrometry imaging (MSI) of volatile metabolites is challenging, especially in matrix-assisted laser desorption/ionization (MALDI). Most MALDI ion sources operate in vacuum, which leads to the vaporization of volatile metabolites during analysis. In addition, tissue samples are often dried during sample preparation, leading to the loss of volatile metabolites even for other MSI techniques. On-tissue chemical derivatization can dramatically reduce the volatility of analytes. Herein a derivatization method is proposed utilizing N,N,N-trimethyl-2-(piperazin-1-yl)ethan-1-aminium iodide to chemically modify short...
Source: Journal of Mass Spectrometry - April 3, 2023 Category: Chemistry Authors: Trevor T. Forsman,
Andrew E. Paulson,
Evan A. Larson,
Torey Looft,
Young Jin Lee Tags: RESEARCH ARTICLE Source Type: research
“Characterization by LC‐MS/MS of oxidized products identified in synthetic peptide Somatostatin and Cetrorelix submitted to forced oxidative stress by Hydrogen Peroxide: Two case studies”
In this study we focused our attention on understanding how oxidative stress performed by H2O2 impacted small synthetic peptides that do not contain residues susceptible to oxidation such as Methionine.Among the amino acids susceptible to oxidation, Methionine is the most reactive and depending on the structure of the protein where it is exposed, it tends to oxidize by converting into Methionine Sulfone or Methionine Sulfoxide by oxidation of its sulfur atom.Scouting experiments obtained by forced oxidative stress conditions are presented on 2 small synthetic peptides which do not contain any Methionine residues spiked wit...
Source: Journal of Mass Spectrometry - April 3, 2023 Category: Chemistry Authors: Antonio Datola,
Alessandra Pistacchio,
Patrizia Simone,
Lucia Colarusso,
Maura Melchiorre,
Gianluca Rinaldi,
Maryam Amidi,
Jane Politi,
Gabriella Angiuoni Tags: RESEARCH ARTICLE Source Type: research
From the Laboratory to the Field: Chemical Analysis of Colored Smoke Pyrotechnic Formulations via Mass Spectrometry Techniques
AbstractSmoke dyes are complex molecular systems that have the potential to form many molecular derivatives and fragments when deployed. The chemical analysis of smoke samples is challenging due to the adiabatic temperature of the pyrotechnic combustion and the molecular complexity of the physically dispersed reaction products. Presented here is the characterization of the reaction byproducts of a simulant Mk124 smoke signal on a multigram scale, which contain the dye disperse red 9 (1-(methylamino)anthraquinone), by ambient ionization mass spectrometry. Our previous work has examined the thermal decomposition of a simplif...
Source: Journal of Mass Spectrometry - March 27, 2023 Category: Chemistry Authors: Patrick W. Fedick,
Kelly M. Thoreson,
Benjamin P. Wilkins,
Douglas M. Papenmeier,
Brian C. Bohrer,
Jonathan M. Dilger Tags: RESEARCH ARTICLE Source Type: research
The influence of matrix concentration and solvent composition on the results of MALDI MSI, with the aid of wet ‐interface matrix deposition
AbstractImaging mass spectrometry is a powerful technique for the molecular analysis of tissue sections. As in many analytical methods, sample preparation is one of the main and most important steps to obtain results of good quality. Usually, the matrix concentration and solvent composition in different studies are taken for granted without any further consideration. In our studies, we aimed to find how matrix concentration and a type of solvent influence the signal. Moreover, we also aimed to find the relationship between these parameters, how they influence the spectra, and how they influence obtained ion maps. In our ex...
Source: Journal of Mass Spectrometry - March 24, 2023 Category: Chemistry Authors: Przemyslaw Mielczarek,
Piotr Suder,
Igor Kotsan,
Anna Bodzon ‐Kulakowska Tags: RESEARCH ARTICLE Source Type: research
The influence of matrix concentration and solvent composition on the results of MALDI ‐MSI, with the aid of wet‐interface matrix deposition
AbstractImaging mass spectrometry is a powerful technique for the molecular analysis of tissue sections. As in many analytical methods, sample preparation is one of the main and most important steps to obtain results of good quality. Usually, the matrix concentration and solvent composition in different studies are taken for granted without any further consideration. In our studies, we aimed to find how matrix concentration and a type of solvent influence the signal. Moreover, what is the relationship between these parameters, and how do they influence the spectra, and obtained ion maps. In our experiments, we used SunColl...
Source: Journal of Mass Spectrometry - March 17, 2023 Category: Chemistry Authors: Przemys ław Mielczarek,
Piotr Suder,
Igor Kotsan,
Anna Bodzon‐Kulakowska Tags: RESEARCH ARTICLE Source Type: research
Impact of wavelength and spot size on laser depth of focus: Considerations for mass spectrometry imaging of non ‐flat samples
AbstractBiospecimens with nearly flat surfaces on a flat stage are typically required for laser-based mass spectrometry imaging (MSI) techniques. However, sampling stages are rarely perfectly level, and accounting for this and the need to accommodate non-flat samples requires a deeper understanding of the laser beam depth of focus. In ablation-based MSI methods, a laser is focused on top of the sample surface, ensuring that the sample is at the focal point or remains within depth of focus. In general, the depth of focus of a given laser is related to the beam quality (M2) and the wavelength (λ). However, because laser is ...
Source: Journal of Mass Spectrometry - March 14, 2023 Category: Chemistry Authors: Alena N. Joignant,
Ying Xi,
David C. Muddiman Tags: TUTORIAL Source Type: research
Localization of cyclopropyl groups and alkenes within glycerophospholipids using gas ‐phase ion/ion chemistry
AbstractShotgun lipid analysis using electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a common approach for the identification and characterization of glycerophohspholipids GPs. ESI-MS/MS, with the aid of collision-induced dissociation (CID), enables the characterization of GP species at the headgroup and fatty acyl sum compositional levels. However, important structural features that are often present, such as carbon –carbon double bond(s) and cyclopropane ring(s), can be difficult to determine. Here, we report the use of gas-phase charge inversion reactions that, in combination with CID, allow for more d...
Source: Journal of Mass Spectrometry - March 14, 2023 Category: Chemistry Authors: De'Shovon M. Shenault,
Scott A. McLuckey,
Elissia T. Franklin Tags: RESEARCH ARTICLE Source Type: research
Evaluation and clinical impact of MALDI Biotyper Mycobacteria Library v6.0 for identification of nontuberculous mycobacteria by MALDI ‐TOF mass spectrometry
AbstractMajor challenges in the identification of non-tuberculous mycobacteria (NTM) by MALDI-TOF MS include protein extraction protocol and updating of the NTM database. The aim of this study was to evaluate MALDI Biotyper Mycobacteria Library v6.0 (Bruker Daltonics GmbH, Bremen, Germany) for identification of clinical NTM isolates and its impact on clinical management. NTM isolates cultivated from clinical samples in 101 patients were identified simultaneously by PCR-reverse hybridization (Hain Lifescience GmbH, Nehren, Germany) as a routinely used reference molecular method and using MALDI Biotyper Microflex LT/SH after...
Source: Journal of Mass Spectrometry - March 10, 2023 Category: Chemistry Authors: Manda Markanovi ć,
Mateja Janković Makek,
Goran Glodić,
Tomislav Kuliš,
Ivana Mareković Tags: RESEARCH ARTICLE Source Type: research
Impact of Wavelength and Spot Size on Laser Depth of Focus: Considerations for Mass Spectrometry Imaging of Non ‐Flat Samples
AbstractBiospecimens with nearly flat surfaces on a flat stage are typically required for laser-based mass spectrometry imaging (MSI) techniques. However, sampling stages are rarely perfectly level, and accounting for this and the need to accommodate non-flat samples requires a deeper understanding of the laser beam depth of focus. In ablation-based MSI methods, a laser is focused on top of the sample surface, ensuring that the sample is at the focal point or remains within depth of focus. In general, the depth of focus of a given laser is related to the beam quality (M2) and the wavelength ( λ). However, since laser is a...
Source: Journal of Mass Spectrometry - February 24, 2023 Category: Chemistry Authors: Alena N. Joignant,
Ying Xi,
David C. Muddiman Tags: TUTORIAL Source Type: research
