Imaging with mass spectrometry: Which ionization technique is best?
AbstractThe use of mass spectrometry (MS) to acquire molecular images of biological tissues and other substrates has developed into an indispensable analytical tool over the past 25 years. Imaging mass spectrometry technologies are widely used today to study the in situ spatial distributions for a variety of analytes. Early MS images were acquired using secondary ion mass spectrometry and matrix-assisted laser desorption/ionization. Researchers have also designed and develop ed other ionization techniques in recent years to probe surfaces and generate MS images, including desorption electrospray ionization (DESI), nanoD...
Source: Journal of Mass Spectrometry - April 17, 2024 Category: Chemistry Authors: Boone M. Prentice Tags: PERSPECTIVE Source Type: research
Enhancing drug development and clinical studies with patient ‐centric sampling using microsampling techniques: Opportunities, challenges, and insights into liquid chromatography‐mass spectrometry strategies
AbstractMicrosampling has revolutionized pharmaceutical drug development and clinical research by reducing sample volume requirements, allowing sample collection at home or nontraditional sites, minimizing animal and patient burden, and enabling more flexible study designs. This perspective paper discusses the transformative impact of microsampling and patient-centric sampling (PCS) techniques, emphasizing their advantages in drug development and clinical trials. We highlight the integration of liquid chromatography-mass spectrometry (LC –MS) strategies for analyzing PCS samples, focusing on our research experience and a...
Source: Journal of Mass Spectrometry - April 16, 2024 Category: Chemistry Authors: Zhuo Chen,
Christopher C. Goudarzi,
Timothy W. Sikorski,
Naidong Weng Tags: PERSPECTIVE Source Type: research
Removal of isobaric interference using pseudo ‐multiple reaction monitoring and energy‐resolved mass spectrometry for the isotope dilution quantification of a tryptic peptide
AbstractEnergy-resolved mass spectrometry (ERMS) and an isotopically labelled internal standard were successfully combined to accurately quantify a tryptic peptide despite the presence of an isobaric interference. For this purpose, electrospray ionisation tandem mass spectrometry (ESI-MS/MS) experiments were conducted into an ion trap instrument using an unconventional 8 m/z broadband isolation window, which encompassed both the tryptic peptide and its internal standard. Interference removal was assessed by determining an excitation voltage that was high enough to maintain a constant value for the analyte/internal standa...
Source: Journal of Mass Spectrometry - April 13, 2024 Category: Chemistry Authors: Alicia Maroto,
Dany Jeanne dit Fouque,
R émy Lartia,
Antony Memboeuf Tags: RESEARCH ARTICLE Source Type: research
From the grapevine to the glass: A wine metabolomics tale by FT ‐ICR‐MS
AbstractWine is one of the most consumed beverages around the world. Its unique characteristics arise from numerous processes, from the selection of grapevine varieties and grapes, the effect of the terroir and geographical origin, through the biochemical process of fermentation by microorganisms, until its aging. All molecules found in wine define its chemical fingerprint and can be used to tell the story of its origin, production, authenticity and quality. Wine's chemical composition can be characterized using an untargeted metabolomics approach based on extreme resolution mass spectrometry. Fourier transform ion cyclotr...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Marta Sousa Silva,
M ónica Soeiro,
Carlos Cordeiro Tags: REVIEW ARTICLE Source Type: research
Simultaneous quantitation of 15 bioactive components in Yupingfeng granules by LC –MS/MS
This study aimed to establish a simple and rapid method by using liquid chromatography –tandem mass spectrometry (LC–MS/MS) to determine 15 bioactive compounds in YPFG. The experimental parameters including extraction methods, extraction solvents, extraction time, solid–liquid ratio, and LC–MS/MS condition were optimized. The linearity, precision, repeatability, stability, and recovery of the established method were evaluated. The contents of 15 bioactive compounds in seven batches of YPFG samples were analyzed by the established method and the results were compared with the values determined by HPLC. The optimal e...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Le ‐yi Huang,
Dao‐feng Chen,
Tong Wu,
Yong‐jian Gao Tags: RESEARCH ARTICLE Source Type: research
High ‐resolution ion mobility separations coupled to mass spectrometry: What's next?
AbstractHerein, I provide a personal perspective on high-resolution multipass ion mobility spectrometry-mass spectrometry (IMS-MS), with a specific emphasis on cyclic (cIMS) and structures for lossless ion manipulations (SLIM IMS)-based separations. My overarching goal for this perspective was to detail what I believe will be the key important areas in which IMS-MS will help shape the bioanalytical community and especially omics-based research. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Gabe Nagy Tags: PERSPECTIVE Source Type: research
Investigating direct current potentials that affect native protein conformation during trapped ion mobility spectrometry –mass spectrometry
AbstractTrapped ion mobility spectrometry –time-of-flight mass spectrometry (TIMS-TOFMS) has emerged as a tool to study protein conformational states. In TIMS, gas-phase ions are guided across the IM stages by applying direct current (DC) potentials (D1–6), which, however, might induce changes in protein structures through collisional a ctivation. To define conditions for native protein analysis, we evaluated the influence of these DC potentials using the metalloenzyme bovine carbonic anhydrase (BCA) as primary test compound. The variation of DC potentials did not change BCA-ion charge and heme content but affected (re...
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Robert L. C. Voeten,
Hany A. Majeed,
Tijmen S. Bos,
Govert W. Somsen,
Rob Haselberg Tags: RESEARCH ARTICLE Source Type: research
Perspective: The complex relationship between charge, mobility, and gas ‐phase protein structure
AbstractIon mobility spectrometry coupled to mass spectrometry (IMS/MS) is a widely used tool for biomolecular separations and structural elucidation. The application of IMS/MS has resulted in exciting developments in structural proteomics and genomics. This perspective gives a brief background of the field, addresses some of the important issues in making structural measurements, and introduces complementary techniques. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Ian K. Webb Tags: PERSPECTIVE Source Type: research
The future role of mass spectrometry in proteomics: Embracing new technologies and building bridges for higher impact
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 12, 2024 Category: Chemistry Authors: Lindsay K. Pino Tags: PERSPECTIVE Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 10, 2024 Category: Chemistry Tags: ISSUE INFORMATION Source Type: research
Quantitative mass spectrometry imaging (qMSI): A tutorial By Russell R. Kibbe and David C. Muddiman
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 9, 2024 Category: Chemistry Authors: Russell R. Kibbe,
David C. Muddiman Tags: COVER STORY Source Type: research
Characterization of PEGylation sites in Neulasta and a biosimilar candidate with a combined fragmentation strategy in mass spectrometry analysis
In this study, we applied a combination of techniques, including post-column addition of triethylamine in reversed-phase liquid chromatography –mass spectrometry (RPLC-MS) to determine the intact molecular mass, and in-source fragmentation (ISF) and higher-energy collision dissociation–tandem mass spectrometry (HCD-MS/MS) to identify the PEGylation site. Our results show that both the pegfilgrastim biosimilar candidate and Neulasta lot s are mono-PEGylated at the N-terminal end. Furthermore, we show that the combined ISF and HCD-MS/MS method can be used for identifying the PEGylation sites in the diPEGylated variant of...
Source: Journal of Mass Spectrometry - April 2, 2024 Category: Chemistry Authors: Navin Rauniyar,
Alyssa J. Togle,
Rachel A. Ronci,
Diego Reyes,
Xuemei Han Tags: RESEARCH ARTICLE Source Type: research
Simplified processing and rapid quantification of buprenorphine, norbuprenorphine, and their conjugated metabolites in human plasma using UPLC –MS/MS: Assessment of buprenorphine exposure during opioid use disorder treatment
AbstractOpioid use disorder (OUD) is a chronic neurobehavioral ailment and is prevalent in pregnancy. OUD is commonly treated with methadone or buprenorphine (BUP). Pregnancy is known to alter the pharmacokinetics of drugs and may lead to changes in drug exposure and response. A simple, specific, and sensitive analytical method for measuring the parent drug and its metabolites is valuable for assessing the impact of pregnancy on drug exposure. A new liquid chromatography –tandem mass spectrometric method that utilized a simple protein precipitation procedure for sample preparation and four deuterated internal standards f...
Source: Journal of Mass Spectrometry - March 20, 2024 Category: Chemistry Authors: Wenchen Zhao,
Osama Y. Alshogran,
Hongfei Zhang,
Anand Joshi,
Elizabeth E. Krans,
Steve Caritis,
Imam H. Shaik,
Raman Venkataramanan Tags: RESEARCH ARTICLE Source Type: research
