A multiparameter optimization in middle ‐down analysis of monoclonal antibodies by LC–MS/MS
AbstractIn antibody-based drug research, a complete characterization of antibody proteoforms covering both the amino acid sequence and all posttranslational modifications remains a major concern. The usual mass spectrometry-based approach to achieve this goal is bottom-up proteomics, which relies on the digestion of antibodies but does not allow the diversity of proteoforms to be assessed. Middle-down and top-down approaches have recently emerged as attractive alternatives but are not yet mastered and thus used in routine by many analytical chemistry laboratories. The work described here aims at providing guidelines to ach...
Source: Journal of Mass Spectrometry - February 23, 2023 Category: Chemistry Authors: Jonathan Dhenin,
Mathieu Dupr é,
Karen Druart,
Alain Krick,
Christine Mauriac,
Julia Chamot‐Rooke Tags: RESEARCH ARTICLE Source Type: research
Quantitative Analysis of Phthalates Using a Pyrolyzer Gas Chromatography/Mass Spectrometry Method
AbstractA pyrolyzer gas chromatography/mass spectrometry (GC/MS) method eliminates toxic solvents that burden our environment and can address the crucial problem of the solvent extraction GC/MS method. The purpose of this study is to establish an efficient quantitative analysis method for 10 phthalates that are regulated by the several governments. A change of concentrations over time for phthalates and internal standards was measured to verify the feasibility of using an auto sampler that facilitates analyzing multiple samples. Both standards maintained constant concentrations over the appropriate time for analysis. A cer...
Source: Journal of Mass Spectrometry - February 21, 2023 Category: Chemistry Authors: Donghyo Lee,
Jungmin Kwon,
Choongryul Choi,
Jinkyung Jeon,
Gyeonghweon Lee Tags: APPLICATION NOTE Source Type: research
Localization of Cyclopropyl Groups and Alkenes Within Glycerophospholipids Using Gas ‐Phase Ion/Ion Chemistry
AbstractShotgun lipid analysis using electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a common approach for the identification and characterization of glycerophohspholipids GPs. ESI-MS/MS, with the aid of collision-induced dissociation (CID), enables the characterization of GP species at the headgroup and fatty acyl sum compositional levels. However, important structural features that are often present, such as carbon-carbon double bond(s) and cyclopropane ring(s), etc., can be difficult to determine. Here, we report the use of gas-phase charge inversion reactions that, in combination with CID, allow for mor...
Source: Journal of Mass Spectrometry - February 21, 2023 Category: Chemistry Authors: De ’ Shovon M. Shenault,
Scott A. McLuckey,
Elissia T. Franklin Tags: RESEARCH ARTICLE Source Type: research
Discriminating Alkylbenzene Isomers with Tandem Mass Spectrometry Using a Dielectric Barrier Discharge Ionization (DBDI) source
AbstractSoft ambient ionization sources generate reactive species that interact with analyte molecules to form intact molecular ions, which allows rapid, sensitive, and direct identification of the molecular mass. We used a dielectric barrier discharge ionization (DBDI) source with nitrogen at atmospheric pressure to detect alkylated aromatic hydrocarbon isomers (C8H10 or C9H12). Intact molecular ions [M]•+ were detected at 2.4 kVpp, but at increased voltage (3.4 kVpp) [M+N]+ ions were formed, which could be used to differentiate regioisomers by CID. At 2.4 kVpp, alkylbenzene isomers with different alkyl-substituents cou...
Source: Journal of Mass Spectrometry - February 14, 2023 Category: Chemistry Authors: Begley Alina,
Zenobi,
Renato Tags: RESEARCH ARTICLE Source Type: research
Time of Acquisition and High Spatial Resolution Mass Spectrometry Imaging
AbstractThe field of mass spectrometry imaging (MSI) is constantly evolving to analyze a diverse array of biological systems. A common goal is the need to resolve cellular and subcellular heterogeneity with high spatial resolution. As the field continues to progress towards high spatial resolution, other parameters must be considered when developing a practical method. Here we discuss the impacts of high spatial resolution on the time of acquisition and the associated implications they have on an MSI analysis (e.g., area of the region of interest). This work presents a brief tutorial serving to evaluate high spatial resolu...
Source: Journal of Mass Spectrometry - February 13, 2023 Category: Chemistry Authors: Mary F. Wang,
Alena N. Joignant,
Alexandria L. Sohn,
Kenneth P. Garrard,
David C. Muddiman Tags: TUTORIAL Source Type: research
Comparison of AMS, TIMS, and SIMS techniques for determining uranium isotope ratios in individual particles
AbstractThe determination of isotope ratios in individual uranium particles is very important for nuclear safeguards. In this work, accelerator mass spectrometry (AMS), thermal ionization mass spectrometry (TIMS), and secondary ion mass spectrometry (SIMS) were applied to isotope ratio analysis of individual uranium particles and compared in terms of background, measurement accuracy, and efficiency. Several individual uranium particles (1 –7 μm) from certified reference materials were used as samples. The results show that the average values of blank counting rate of235U for AMS, FT-TIMS (FT: fission track), SEM-TIMS (...
Source: Journal of Mass Spectrometry - February 13, 2023 Category: Chemistry Authors: Jie Gao,
Ming He,
Yan Shen,
Chang ‐Kun Xu,
Yong‐Gang Zhao Tags: RESEARCH ARTICLE Source Type: research
A multi ‐parameter optimization in middle‐down analysis of monoclonal antibodies by LC‐MS/MS
AbstractIn antibody-based drug research, a complete characterization of antibody proteoforms covering both the amino acid sequence and all post-translational modifications remains a major concern. The usual mass spectrometry-based approach to achieve this goal is bottom-up proteomics, which relies on the digestion of antibodies, but does not allow the diversity of proteoforms to be assessed. Middle-down and top-down approaches have recently emerged as attractive alternatives but are not yet mastered and thus used in routine by many analytical chemistry laboratories. The work described here aims at providing guidelines to a...
Source: Journal of Mass Spectrometry - February 9, 2023 Category: Chemistry Authors: Jonathan Dhenin,
Mathieu Dupr é,
Karen Druart,
Alain Krick,
Christine Mauriac,
Julia Chamot‐Rooke Tags: RESEARCH ARTICLE Source Type: research
Quantitative proteomics I.: Concept, design, and planning of quantitative proteomics experiments
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - February 7, 2023 Category: Chemistry Authors: Sug ár Simon,
Drahos Laszlo,
Vekey Karoly Tags: TUTORIAL Source Type: research
Glycomics by ion mobility tandem mass spectrometry of chondroitin sulfate disaccharide domain in biglycan
In this study, we have implemented an advanced glycomics method based on ion mobility separation (IMS) mass spectrometry (MS) and tandem MS (MS/MS) to characterize the CS disaccharide domains in BGN. The high separation efficiency and sensitivity of this technique allowed the discrimination of five distinct CS disaccharide motifs, of which four irregulated in their sulfation pattern. For the first time, trisulfated unsaturated and bisulfated saturated disaccharides were found in BGN, the latter species documenting the non-reducing end of the chains. The structural investigation by IMS MS/MS disclosed that in one or both of...
Source: Journal of Mass Spectrometry - February 5, 2023 Category: Chemistry Authors: Mirela Sarbu,
Raluca Ica,
Edie Sharon,
David E. Clemmer,
Alina D. Zamfir Tags: RESEARCH ARTICLE Source Type: research
