Tracking the disulfide rearrangement of heated lactoglobulin by matrix ‐assisted laser desorption/ionization‐in‐source decay top‐down analysis
AbstractDisulfide bond rearrangement is a common occurrence during protein analysis or treatment. A convenient and rapid method has been developed to investigate heat-induced disulfide rearrangement of lactoglobulin using matrix-assisted laser desorption/ionization-in-source decay (MALDI-ISD) technology. By analyzing heated lactoglobulin in reflectron and linear mode, we demonstrated that cysteines C66 and C160 exist as free residues other than linked ones in some protein isomers. This method provides a straightforward and expeditious way to assess the cysteine status and structural changes of proteins under heat stress. (...
Source: Journal of Mass Spectrometry - May 2, 2023 Category: Chemistry Authors: Xiaoyun Wei,
Peiqi Luo,
Lingpeng Zhan Tags: APPLICATION NOTE Source Type: research
Ready Biodegradability study and insights with UHPLC ‐qToF of a Metformin‐based drug and of Metarecod, a natural substance‐based medical device
AbstractDrugs are indispensable products with incontrovertible benefits to human health and lifestyle. However, due to their overuse and improper disposal, unwanted residues of Active Pharmaceutical Ingredients (APIs) have been found in different compartments of the environment and now are considered as Contaminants of Emerging Concern (CEC). Therefore, they are very likely to have a boomerang effect on human health, because they can enter into the food cycle. In the current legislation framework one of the test first used to evaluate biodegradation of APIs as well as chemical compounds is the ready biodegradability test (...
Source: Journal of Mass Spectrometry - May 2, 2023 Category: Chemistry Authors: Giacomo Proietti,
Michela Burico,
Claudio Marzio Quintiero,
Emiliano Giovagnoni,
Valentino Mercati,
Mattia Gianni,
Luisa Mattoli Tags: RESEARCH ARTICLE Source Type: research
Systemic chemical characterization of Lemna minor by UHPLC ‐Q‐Exactive Orbitrap MS coupled with parallel reaction monitoring
In this study, the UHPLC-Q-Exactive Orbitrap mass spectrometry coupled with parallel reaction monitoring was established. Finally, a total of 112 constituents including 30 dipeptides, 28 nucleosides, 11 amino acids, 10 organic acids, 10 flavonoids, and 23 other compounds were identified by MS, diagnostic fragment ions and retention time. 101 of those chemicals were first found from LM, which was very benefit for the further development and utilization in nutriment and medicinal use of LM. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 28, 2023 Category: Chemistry Authors: Min Zhang,
Qing Li,
Kaiquan Yu,
Jiaxin Li,
Jili Wu,
Shani Li,
Yuqi Chen,
Wei Cai,
Jieyao Ma Tags: RESEARCH ARTICLE Source Type: research
Correction to “On the importance of color in mass spectrometry imaging”
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 27, 2023 Category: Chemistry Authors: David C. Muddiman Tags: CORRECTION Source Type: research
Correction to “Time of acquisition and high spatial resolution mass spectrometry imaging”
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 27, 2023 Category: Chemistry Authors: David C. Muddiman Tags: CORRECTION Source Type: research
On ‐tissue chemical derivatization of volatile metabolites for matrix‐assisted laser desorption/ionization mass spectrometry imaging
AbstractMass spectrometry imaging (MSI) of volatile metabolites is challenging, especially in matrix-assisted laser desorption/ionization (MALDI). Most MALDI ion sources operate in vacuum, which leads to the vaporization of volatile metabolites during analysis. In addition, tissue samples are often dried during sample preparation, leading to the loss of volatile metabolites even for other MSI techniques. On-tissue chemical derivatization can dramatically reduce the volatility of analytes. Herein, a derivatization method is proposed utilizing N,N,N-trimethyl-2-(piperazin-1-yl)ethan-1-aminium iodide to chemically modify shor...
Source: Journal of Mass Spectrometry - April 13, 2023 Category: Chemistry Authors: Trevor T. Forsman,
Andrew E. Paulson,
Evan A. Larson,
Torey Looft,
Young Jin Lee Tags: RESEARCH ARTICLE Source Type: research
Tracking the disulfide rearrangement of heated lactoglobulin by MALDI ‐ISD top‐down analysis
AbstractDisulfide bond rearrangement is a common occurrence during protein analysis or treatment. A convenient and rapid method has been developed to investigate heat-induced disulfide rearrangement of lactoglobulin, using matrix-assisted laser desorption/ionization-in source decay (MALDI-ISD) technology. By analyzing heated lactoglobulin in reflectron and linear mode, we demonstrated that cysteines C66 and C160 exist as free residues other than linked ones in some protein isomers. This method provides a straightforward and expeditious way to assess the cysteine status and structural changes of proteins under heat stress. ...
Source: Journal of Mass Spectrometry - April 10, 2023 Category: Chemistry Authors: Xiaoyun Wei,
Peiqi Luo,
Lingpeng Zhan Tags: APPLICATION NOTE Source Type: research
Quantitative proteomics I.: Concept, design, and planning of quantitative proteomics experiments By Simon Sug ár, Laszlo Drahos and Karoly Vekey
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 5, 2023 Category: Chemistry Authors: Simon Sug ár,
László Drahos,
Károly Vékey Tags: COVER STORY Source Type: research
Cover Picture
(Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - April 5, 2023 Category: Chemistry Tags: COVER PICTURE Source Type: research
On ‐Tissue Chemical Derivatization of Volatile Metabolites for Matrix Assisted Laser Desorption/Ionization
AbstractMass spectrometry imaging (MSI) of volatile metabolites is challenging, especially in matrix-assisted laser desorption/ionization (MALDI). Most MALDI ion sources operate in vacuum, which leads to the vaporization of volatile metabolites during analysis. In addition, tissue samples are often dried during sample preparation, leading to the loss of volatile metabolites even for other MSI techniques. On-tissue chemical derivatization can dramatically reduce the volatility of analytes. Herein a derivatization method is proposed utilizing N,N,N-trimethyl-2-(piperazin-1-yl)ethan-1-aminium iodide to chemically modify short...
Source: Journal of Mass Spectrometry - April 3, 2023 Category: Chemistry Authors: Trevor T. Forsman,
Andrew E. Paulson,
Evan A. Larson,
Torey Looft,
Young Jin Lee Tags: RESEARCH ARTICLE Source Type: research
“Characterization by LC‐MS/MS of oxidized products identified in synthetic peptide Somatostatin and Cetrorelix submitted to forced oxidative stress by Hydrogen Peroxide: Two case studies”
In this study we focused our attention on understanding how oxidative stress performed by H2O2 impacted small synthetic peptides that do not contain residues susceptible to oxidation such as Methionine.Among the amino acids susceptible to oxidation, Methionine is the most reactive and depending on the structure of the protein where it is exposed, it tends to oxidize by converting into Methionine Sulfone or Methionine Sulfoxide by oxidation of its sulfur atom.Scouting experiments obtained by forced oxidative stress conditions are presented on 2 small synthetic peptides which do not contain any Methionine residues spiked wit...
Source: Journal of Mass Spectrometry - April 3, 2023 Category: Chemistry Authors: Antonio Datola,
Alessandra Pistacchio,
Patrizia Simone,
Lucia Colarusso,
Maura Melchiorre,
Gianluca Rinaldi,
Maryam Amidi,
Jane Politi,
Gabriella Angiuoni Tags: RESEARCH ARTICLE Source Type: research
