A pharmacokinetic study to correlate the hypoglycemic effect of phlorizin in rats: Identification of metabolites as inhibitors of sodium/glucose cotransporters
AbstractPhlorizin (PRZ) is a natural product that belongs to a class of dihydrochalcones. The unique pharmacological property of PRZ is to block glucose absorption or reabsorption through specific and competitive inhibitors of the sodium/glucose cotransporters (SGLTs) in the intestine (SGLT1) and kidney (SGLT2). This results in glycosuria by inhibiting renal reabsorption of glucose and can be used as an adjuvant treatment for type 2 diabetes. The pharmacokinetic profile, metabolites of the PRZ, and efficacy of metabolites towards SGLTs are unknown. Therefore, the present study on the characterization of hitherto unknown in...
Source: Journal of Mass Spectrometry - July 19, 2023 Category: Chemistry Authors: Roshan M. Borkar,
Abhinav Kanwal,
Bandu Raju,
Sai Sharanya Pulimamidi,
Agneesh Pratim Das,
Subhash Mohan Agarwal,
Sanjay K. Banerjee,
Ragampeta Srinivas Tags: RESEARCH ARTICLE Source Type: research
A practical method for rapid discrimination of constituents in Psoraleae Fructus by UPLC ‐Q‐Orbitrap MS
This study established a UPLC-Q-Orbitrap MS method for the separation and discrimination of the chemical constituents of PF, which can lay a foundation for the further study of its medicinal substances and quality control. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - July 19, 2023 Category: Chemistry Authors: Yanyan Chen,
Luhuan Tang,
Mengru Wu,
Lexin Shu,
Yanyan Xu,
Yaqi Yao,
Yubo Li Tags: RESEARCH ARTICLE Source Type: research
Optimization of reversed ‐phase solid‐phase extraction for shotgun proteomics analysis
AbstractReversed-phase solid-phase extraction (SPE) is the method of choice for the purification of proteomics samples. Even though the efficacy of SPE methods is sample type-dependent, the manufacturers' protocols are used in most studies. Using an optimized SPE method can lead to a substantial gain in identification and recovery. In this tutorial, we give a brief introduction to the most important parameters influencing SPE performance, and we present a short workflow (16 measurements) for optimizing the SPE procedure. This is complemented by method performance assessment instructions and a short troubleshooting guide to...
Source: Journal of Mass Spectrometry - July 19, 2023 Category: Chemistry Authors: Fanni Bugyi,
Lilla Turi ák,
László Drahos,
Gábor Tóth Tags: TUTORIAL Source Type: research
The pivotal role of high ‐resolution mass spectrometry in the study of grape glycosidic volatile precursors for the selection of grapevines resistant to mildews
AbstractA breeding program to produce new grape varieties tolerant to main vine fungal pathogens (Plasmopara viticola andErysiphe necator) is carrying out by crossingVitis vinifera cv. “Glera” with resistant genotypes such as “Solaris,” “Bronner,” and “Kunleany.” Firstly, resistance gene-based markers analyses allowed the identification of five genotypes, which have inherited the resistance loci against mildews. To select those that also inherited the phenotype as close as possible to ‘Glera’ suitable to be introduced in the Prosecco wine production protocols, the grape glycosidic derivatives were studi...
Source: Journal of Mass Spectrometry - July 18, 2023 Category: Chemistry Authors: Mirko De Rosso,
Annarita Panighel,
Daniele Migliaro,
Tyrone Possamai,
Fabiola De Marchi,
Riccardo Velasco,
Riccardo Flamini Tags: SPECIAL ISSUE ‐ RESEARCH ARTICLE Source Type: research
Authenticity study of commercial samples of St. John's wort by paper spray ionization mass spectrometry and chemometric tools
AbstractHypericum perforatum L. (St. John's wort) is one of the world's most consumed medicinal plants for treating depression and psychiatric disorders. Counterfeiting can occur in the medicinal plant trade, either due to the lack of active ingredients or the addition of substances not mentioned on the labels, often without therapeutic value or even harmful to health. Hence, 43 samples of St. John's wort commercially acquired in different Brazilian regions and other countries were analyzed by paper spray ionization mass spectrometry (PS-MS) and modeled by principal component analysis. Hence, samples (plants, capsules, and...
Source: Journal of Mass Spectrometry - July 13, 2023 Category: Chemistry Authors: Ana Gabriella Carvalho Miguita,
Rodinei Augusti,
Marcelo Martins Sena,
Cl ésia Cristina Nascentes Tags: RESEARCH ARTICLE Source Type: research
Characteristic response of formulation ingredients revealed by ultra high performance liquid chromatography ‐electrospray ionization‐high resolution mass spectrometry‐based untargeted screening of pesticides in soil
AbstractCommercial solutions of pesticides consist of two main components: The active substance and the formulation ingredients. These ingredients, mainly composed of polymeric surfactants, are considered inert vis- à-vis the targeted organisms and nature. Nonetheless, a relatively low attention is given to their analysis and fate tracking in the environment. In this context, the current paper, embedded in a large study of fate and impact of formulated pesticides in soil, focuses on the analysis of these formu lation ingredients. It mainly highlights and discusses the characteristic response of these ingredients in liquid...
Source: Journal of Mass Spectrometry - July 11, 2023 Category: Chemistry Authors: Hikmat Ghosson,
Delphine Raviglione,
Marie ‐Virginie Salvia,
Cédric Bertrand Tags: RESEARCH ARTICLE Source Type: research
Quantification of pharmaceutical compounds in tissue and plasma samples using selective ion accumulation with multiple mass isolation windows
AbstractQuantification of pharmaceutical compounds using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is an alternative to traditional liquid chromatography (LC)-MS techniques. Benefits of MALDI-based approaches include rapid analysis times for liquid samples and imaging mass spectrometry capabilities for tissue samples. As in most quantification experiments, the use of internal standards can compensate for spot-to-spot and shot-to-shot variability associated with MALDI sampling. However, the lack of chromatographic separation in traditional MALDI analyses results in diminished peak capacity d...
Source: Journal of Mass Spectrometry - July 11, 2023 Category: Chemistry Authors: Zhongling Liang,
Boone M. Prentice Tags: RESEARCH ARTICLE Source Type: research
Official controls for the determination of lipophilic marine biotoxins in mussels farmed along the Adriatic coast of Central Italy
AbstractIn the present study, 334 samples of mussels (Mytilus galloprovincialis) harvested along the coasts of the Central Adriatic Sea during the years 2020 –2021 were analyzed for the presence of lipophilic marine biotoxins according to the European Harmonized Standard Operating Procedure. The results showed that 74 (22%) and 84 (25%) samples were positive to okadaic acid and yessotoxin groups, respectively. Among them, only 11 (3.3%) samples resulte d as non-compliant, as they exceeded the maximum limits (160 μg okadaic acid equivalent/kg) established by the Regulation (EC) 853/2004. The method applied in this s...
Source: Journal of Mass Spectrometry - July 11, 2023 Category: Chemistry Authors: Loredana Annunziata,
Rosaria Aloia,
Giampiero Scortichini,
Pierina Visciano Tags: SPECIAL ISSUE ‐ RESEARCH ARTICLE Source Type: research
Optimum collision energies for proteomics: The impact of ion mobility separation
AbstractIon mobility spectrometry (IMS) is a widespread separation technique used in various research fields. It can be coupled to liquid chromatography –mass spectrometry (LC–MS/MS) methods providing an additional separation dimension. During IMS, ions are subjected to multiple collisions with buffer gas, which may cause significant ion heating. The present project addresses this phenomenon from the bottom-up proteomics point of view. We perfor med LC–MS/MS measurements on a cyclic ion mobility mass spectrometer with varied collision energy (CE) settings both with and without IMS. We investigated the CE dependence o...
Source: Journal of Mass Spectrometry - July 7, 2023 Category: Chemistry Authors: Kinga Nagy,
Gabriella Gell én,
Dávid Papp,
Gitta Schlosser,
Ágnes Révész Tags: RESEARCH ARTICLE Source Type: research
