Assessing the performance of a targeted absolute quantification isotope dilution liquid chromatograhy tandem mass spectrometry assay versus a commercial nontargeted relative quantification assay for detection of three major perfluoroalkyls in human blood
AbstractIsotope dilution ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC –MS/MS) is commonly used for trace analysis of polyfluoroalkyl and perfluoroalkyl substances (PFAS) in difficult matrices. Commercial nontargeted analysis of major PFAS where relative concentrations are obtained cost effectively is rapidly emerging and is claimed to provide comparable results to t hat of absolute quantification using matrix matched calibration and isotope dilution UHPLC–MS/MS. However, this remains to be demonstrated on a large scale. We aimed to assess the performance of a targeted absolute ...
Source: Journal of Mass Spectrometry - January 23, 2024 Category: Chemistry Authors: Samira Salihovic,
Linda Dunder,
Monica Lind,
Lars Lind Tags: RAPID COMMUNICATION Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - January 22, 2024 Category: Chemistry Tags: ISSUE INFORMATION Source Type: research
One ‐step functionalization of paper and simplified antibody immobilization for on‐the‐spot immunocapture from dried serum in liquid chromatography‐tandem mass spectrometry based targeted protein determination
AbstractThis work aimed to simplify and improve the process of binding monoclonal antibodies (mAbs) covalently to filter paper for use in dried blood spot sampling, enabling instant capture of protein biomarkers for targeted protein determination. Incorporating the necessary immunocapture sample preparation step in the initial sampling stage saves time and reduces the workload. The biomarker human chorionic gonadotropin (hCG) was used as the model analyte. The antibody-based paper samplers were prepared by functionalizing paper discs (6 mm) through a simple reaction using divinyl sulfone (DVS). After DVS activation, the ...
Source: Journal of Mass Spectrometry - January 11, 2024 Category: Chemistry Authors: Ago Mrsa,
Greta Nardini,
Trine Gr ønhaug Halvorsen,
Bernd Thiede,
Léon Reubsaet Tags: RESEARCH ARTICLE Source Type: research
Chromatographic –mass spectrometric analysis of peptidic analytes (2–10 kDa) in doping control urine samples
AbstractPeptides with a molecular mass between 2 and 10 kDa that are prohibited in elite sports usually require dedicated sample preparation and mass spectrometric detection that commonly cannot be combined with other (lower molecular mass) substances. In most instances, the physicochemical differences are too significant to allow for a generic analyti cal procedure. A simplification of established and comparably complex analytical approaches is therefore desirable and has been accomplished in the context of this study. With urine samples representing still the most frequently collected doping control specimens, efficien...
Source: Journal of Mass Spectrometry - January 10, 2024 Category: Chemistry Authors: Andreas Thomas,
Katja Walpurgis,
Mario Thevis Tags: RESEARCH ARTICLE Source Type: research
Combining surface ‐enhanced Raman spectroscopy and paper spray mass spectrometry for the identification and confirmation of psychotropic substances in alcoholic beverages
AbstractCriminal practices in which an individual becomes vulnerable and prone to sexual assault after ingesting drinks spiked with doping substances have become a social concern globally. As forensic protocols require a multi-tiered strategy for chemical evidentiary analysis, the backlog of evidence has become a significant problem in the community. Herein, a fast, sensible, and complementary dual analytical methodology was developed using a single commercial paper substrate for surface-enhanced Raman spectroscopy (SERS) and paper spray mass spectrometry (PS-MS) analysis to identify psychotropic substances added to alcoho...
Source: Journal of Mass Spectrometry - December 26, 2023 Category: Chemistry Authors: Marina Jurisch,
Cristiano Fantini,
Rodinei Augusti,
Mariana Ramos Almeida Tags: RESEARCH ARTICLE Source Type: research
Does deprotonated benzoic acid lose carbon monoxide in collision ‐induced dissociation?
AbstractDecarboxylation is known to be the major fragmentation pathway for the deprotonated carboxylic acids in collision-induced dissociation (CID). However, in the CID mass spectrum of deprotonated benzoic acid (m/z 121) recorded on a Q-orbitrap mass spectrometer, the dominant peak was found to bem/z 93 instead of the anticipatedm/z 77. Based on theoretical calculations,18O-isotope labeling and MS3 experiments, we demonstrated that the fragmentation of benzoate anion begins with decarboxylation, but the initial phenide anion (m/z 77) can react with trace O2 in the mass analyzer to produce phenolate anion (m/z 93) and oth...
Source: Journal of Mass Spectrometry - December 26, 2023 Category: Chemistry Authors: Yingying Liu,
Xue Wang,
Danyang Zhang,
Chen Wang,
Haijiao Xie,
Hongping Chen,
Yunfeng Chai Tags: APPLICATION NOTE Source Type: research
Prototyping an ionization source for non ‐engineers
AbstractNovel mass spectrometry (MS) based analytical platforms have enabled scientists to detect and quantify molecules within biological and environmental samples more accurately. Novel MS instrumentation starts as a prototype and, after years of development, can become a commercial product to be used by the larger MS community. Without the initial prototype, many MS-based instruments today would not be produced. Additionally, biotechnology companies are the main drivers for research, development, and production of novel instruments, but the tools for prototyping instrumentation have never been more accessible. Here, we ...
Source: Journal of Mass Spectrometry - December 22, 2023 Category: Chemistry Authors: Kevan T. Knizner,
Seth M. Eisenberg,
David C. Muddiman Tags: TUTORIAL Source Type: research
Advancing the analytical toolkit in the investigation of vector mosquito host biting site selection
AbstractHigh-resolution mass spectrometry and ion mobility spectrometry provide additional confidence in biological marker discovery and elucidation by adding additional peak capacity through physiochemical separation orthogonal to chromatography. Sophisticated analytical techniques have proved valuable in the identification of human skin surface chemicals used by vector mosquitoes to find their human host. Polydimethylsiloxane (PDMS) was used as a non-invasive passive wearable sampler to concentrate skin surface non-volatile and semi-volatile compounds prior to solvent desorption directly in an LC vial, thereby simplifyin...
Source: Journal of Mass Spectrometry - December 19, 2023 Category: Chemistry Authors: Madelien Wooding,
Tyren Dodgen,
Egmont R. Rohwer,
Yvette Naud é Tags: RESEARCH ARTICLE Source Type: research
A new absolute quantitative method for peptide and metabolite detection
AbstractMass spectrometry is widely employed in various analytical fields for both compound identification and quantification. While in the case of compound identification, the high-resolution instrument has increased selectivity and characterization efficiency; in the case of quantitative analysis, some critical tasks actually remain. In particular, different compounds exhibit different ionization efficiency, and this introduces the need to have a calibration standard for each analyte. In this paper, we present a new elaborative data technology, which makes it possible to standardize calibration between different instrume...
Source: Journal of Mass Spectrometry - December 18, 2023 Category: Chemistry Authors: Carlo Brogna,
Simone Cristoni Tags: RESEARCH ARTICLE Source Type: research
Performance of collagen ‐based matrices from Nile tilapia skin: A pilot proteomic study in a murine model of wound healing
AbstractFull-thickness cutaneous trauma, due to the lack of dermis, leads to difficulty in epithelialization by keratinocytes, developing a fibrotic scar, with less elasticity than the original skin, which may have disorders in predisposed individuals, resulting in hypertrophic scar and keloids. Biomedical materials have excellent characteristics, such as good biocompatibility and low immunogenicity, which can temporarily replace traditional materials used as primary dressings. In this work, we developed two dermal matrices based on Nile tilapia collagen, with (M_GAG) and without (M) glycosaminoglycans, using a sugarcane p...
Source: Journal of Mass Spectrometry - December 18, 2023 Category: Chemistry Authors: Cl áudia B. A. Medeiros,
Iasmim Lopes de Lima,
Thiago Barbosa Cahú,
Bruna R. Muniz,
Maria Helena M. L. Ribeiro,
Érico Higino de Carvalho,
Marcos Nogueira Eberlin,
Marcelo J. B. Miranda,
Ranilson de Souza Bezerra,
Roberto Afonso da Silva,
Jos Tags: RESEARCH ARTICLE Source Type: research
An analytical approach for on ‐site analysis of breath samples for Δ9‐tetrahydrocannabinol (THC)
AbstractIncreased acceptance of cannabis containing the psychoactive component, Δ9-tetrahydrocannabinol (THC), raises concerns about the potential for impaired drivers and increased highway accidents. In contrast to the “breathalyzer” test, which is generally accepted for determining the alcohol level in a driver, there is no currently accepted roadside test for THC in a m otorist. There is a need for an easily collectible biological sample from a potentially impaired driver coupled with an accurate on-site test to measure the presence and quantity of THC in a driver. A novel breath collection device is described, whi...
Source: Journal of Mass Spectrometry - December 18, 2023 Category: Chemistry Authors: Jack Henion,
Changtong Hao,
Daniel Eikel,
Olof Beck,
Peter Stambeck Tags: RESEARCH ARTICLE Source Type: research
LC –MS/MS analysis of coccidiostats in meat supply chain safety
AbstractThe presence of coccidiostats in meat products represents an important topic because of the animal administration of these substances, authorized as feed additives for targeted species, in order to prevent and inhibit coccidiosis. Coccidiostats include both ionophores and synthetic molecules characterized by different chemical –physical properties such as polarity. Meat is a matrix characterized by many interfering compound groups, such as proteins, phospholipids, and fats. High-performance liquid chromatography (HPLC) coupled to mass spectrometry (MS) analysis allows the required selectivity and sensitivity for ...
Source: Journal of Mass Spectrometry - December 18, 2023 Category: Chemistry Authors: Claudia Ancillotti,
Lisa Bonciani,
Davide Passerini,
Giulia Scanavini,
Roberto Riccio Tags: RESEARCH ARTICLE Source Type: research
Software ‐assisted automated detection and identification of “unknown” fentanyl analogues
AbstractFentanyl and its non-pharmaceutical analogues (NPFs) are potent synthetic opioids, traditionally used for pain management, with ever-increasing illicit uses. Tightening the regulation for known fentanyls leads to new synthetic analogues in the opioid market. Furthermore, the Organization for the Prohibition of Chemical Weapons (OPCW) has recently issued a decision regarding aerosolized use of central nervous system (CNS)-acting agents, such as fentanyl and its analogues, under the concern that these materials could be misused for terror or war purposes. The ever-increasing development of new fentanyl analogues make...
Source: Journal of Mass Spectrometry - December 18, 2023 Category: Chemistry Authors: Eyal Drug,
Dana Marder,
Iris Binyamin,
Dina Yeffet,
Eytan Gershonov,
Shai Dagan Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Mass Spectrometry)
Source: Journal of Mass Spectrometry - December 12, 2023 Category: Chemistry Tags: ISSUE INFORMATION Source Type: research
